##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547836_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2595271
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07856173786861	33.0	31.0	34.0	31.0	34.0
2	32.528025011646186	34.0	31.0	34.0	31.0	34.0
3	32.6605961381297	34.0	31.0	34.0	31.0	34.0
4	36.170258905524705	37.0	35.0	37.0	35.0	37.0
5	36.142291113336526	37.0	35.0	37.0	35.0	37.0
6	36.20142328103693	37.0	37.0	37.0	35.0	37.0
7	36.39331923332862	37.0	37.0	37.0	35.0	37.0
8	36.394727949412605	37.0	37.0	37.0	35.0	37.0
9	38.19825020200203	39.0	39.0	39.0	37.0	39.0
10-11	37.77743210631954	39.0	37.5	39.0	35.0	39.0
12-13	37.341277461968325	39.0	37.0	39.0	34.5	39.0
14-15	38.59959441615153	40.0	38.0	41.0	34.5	41.0
16-17	38.70199470498457	40.0	38.0	41.0	35.0	41.0
18-19	38.576356765825224	40.0	37.5	41.0	35.0	41.0
20-21	38.45012177918992	40.0	37.0	41.0	34.0	41.0
22-23	38.27322734311754	40.0	36.5	41.0	34.0	41.0
24-25	38.25483119103939	40.0	36.0	41.0	34.0	41.0
26-27	38.263321055874314	40.0	36.0	41.0	34.0	41.0
28-29	38.18139936060627	40.0	36.0	41.0	34.0	41.0
30-31	38.05290950347767	40.0	36.0	41.0	34.0	41.0
32-33	37.79363812102859	40.0	36.0	41.0	33.5	41.0
34-35	37.53380417690484	40.0	36.0	41.0	33.0	41.0
36-37	37.37099247053583	40.0	36.0	41.0	33.0	41.0
38-39	37.27857919269317	40.0	35.0	41.0	33.0	41.0
40-41	37.18297607456023	40.0	35.0	41.0	32.0	41.0
42-43	37.13172689865529	40.0	35.0	41.0	32.0	41.0
44-45	37.00712411150897	40.0	35.0	41.0	32.0	41.0
46-47	36.887489398987626	40.0	35.0	41.0	32.0	41.0
48-49	36.77352211002243	39.0	35.0	41.0	31.0	41.0
50-51	36.56494119496577	39.0	35.0	41.0	31.0	41.0
52-53	36.37909509257415	39.0	35.0	40.5	31.0	41.0
54-55	36.19490951041337	38.5	35.0	40.0	31.0	41.0
56-57	35.91595232251275	38.0	35.0	40.0	30.0	41.0
58-59	35.68924805925855	37.5	35.0	40.0	30.0	41.0
60-61	35.342312228665136	37.0	35.0	40.0	29.0	41.0
62-63	35.005859889005805	36.5	34.0	39.5	28.5	41.0
64-65	34.67242804316004	36.0	34.0	39.0	28.0	40.5
66-67	34.37129629237178	35.5	34.0	39.0	28.0	40.0
68-69	33.90148832241412	35.0	34.0	38.0	26.5	40.0
70-71	33.602358096707434	35.0	34.0	37.0	26.0	39.5
72-73	33.32482523019754	35.0	34.0	37.0	26.0	39.0
74-75	33.010429161347695	35.0	33.0	36.0	26.0	39.0
76-77	31.03042668761759	33.5	30.0	34.5	23.0	36.0
78-79	32.1303553655861	34.5	32.5	35.0	25.0	37.0
80-81	32.22242166617667	35.0	33.0	35.0	25.0	37.0
82-83	32.0284669693454	35.0	33.0	35.0	24.5	36.0
84-85	31.802850646425746	35.0	33.0	35.0	24.0	36.0
86	31.364324958742266	34.0	32.0	35.0	22.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	5.0
10	7.0
11	26.0
12	33.0
13	95.0
14	284.0
15	804.0
16	2015.0
17	4543.0
18	10438.0
19	16373.0
20	19432.0
21	18883.0
22	15828.0
23	13662.0
24	12982.0
25	14439.0
26	17108.0
27	20705.0
28	25932.0
29	32908.0
30	42078.0
31	53320.0
32	67948.0
33	91605.0
34	211075.0
35	206796.0
36	226692.0
37	445335.0
38	697514.0
39	325579.0
40	820.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.616852344128993	24.428893938243828	30.56008409141088	13.394169626216298
2	30.562627178433388	25.737697527541442	31.23889566831364	12.460779625711535
3	29.652124961131225	24.14907730252448	33.4815131059531	12.717284630391202
4	27.033670086861832	27.20320922169592	31.673532359433754	14.089588332008487
5	24.21469665402958	32.2281950516921	30.251445802769727	13.305662491508594
6	22.69604985375323	41.10537974646964	25.955786505532565	10.24278389424457
7	89.50163585999304	3.258349513403417	5.789337606747041	1.4506770198565004
8	90.29434691020707	2.9080585418632583	5.528247339102545	1.2693472088271323
9	85.45446699015248	5.922233169484035	6.670517260047217	1.9527825803162753
10-11	33.926977182729665	30.895501857031498	22.65620815706722	12.521312803171615
12-13	27.705834959046665	24.836963076303014	29.139461736365874	18.317740228284446
14-15	19.52158368047113	30.394648574272203	32.26851839364752	17.81524935160914
16-17	24.185797937864677	28.574780822503698	29.744388928940367	17.495032310691254
18-19	24.270837226632594	29.690271266468898	28.701164541198203	17.337726965700305
20-21	26.579093281587934	27.99963857338983	29.232554134038413	16.188714010983823
22-23	23.061946132022438	24.35418112405217	32.3954993524761	20.188373391449293
24-25	20.884293008321674	28.94591740130414	31.481278833694056	18.68851075668013
26-27	20.262007320237462	32.34999350742177	28.903937199621925	18.48406197271884
28-29	18.886987139300672	29.2207056604108	32.845356034109734	19.04695116617879
30-31	22.236406140245084	27.8894188699369	30.712457388843017	19.161717600975003
32-33	23.304213702538192	28.456931857983232	29.151522133912028	19.087332305566548
34-35	20.854353938374835	28.552894861461482	30.21064081554489	20.382110384618795
36-37	22.315415230239925	28.89553730612333	29.63260869481453	19.156438768822216
38-39	20.99241273839996	29.95411654505445	28.45741350325265	20.596057213292948
40-41	21.45161333826024	27.910746122466595	28.86282395942466	21.7748165798485
42-43	21.36106402761022	27.977733346536837	28.552104963219644	22.109097662633307
44-45	20.911939446786096	29.47087221334497	28.579423882900862	21.037764456968077
46-47	21.57450994520418	29.091181614559712	28.717579011979865	20.61672942825624
48-49	21.3400835596745	28.233718174325535	29.97752450514802	20.44867376085195
50-51	19.903104531280164	29.694355618353534	29.605328306754863	20.79721154361144
52-53	21.722355777103814	27.347336752115677	29.72090390560369	21.209403565176814
54-55	21.569500834402263	27.70489093431861	29.97586764542123	20.749740585857893
56-57	21.611057188247393	27.697743318520494	29.001402936340753	21.68979655689136
58-59	22.45345476445427	28.109954605896647	29.077734078637647	20.358856551011435
60-61	23.086317382654837	28.98776274231092	27.961858318456915	19.964061556577327
62-63	22.495878079784344	27.33375435551817	29.656401971123632	20.51396559357385
64-65	21.625776267680717	29.31713104334769	28.625315044170723	20.43177764480087
66-67	20.902576262748667	29.196488536264614	27.522829022479733	22.378106178506986
68-69	20.178220309170026	30.367425983644868	27.76946993204178	21.68488377514333
70-71	20.984205503009125	29.20153617868808	28.886328248572113	20.927930069730674
72-73	22.037428846544348	29.421859990729292	28.011987958097635	20.528723204628726
74-75	21.021966492131263	30.288609551757794	27.17716184552596	21.512262110584984
76-77	21.991075305815848	28.818531860449255	28.05179112316209	21.138601710572807
78-79	20.20413282466455	28.95755395101321	28.978592216381255	21.859721007940983
80-81	19.557244696218625	28.576630340338255	29.25811986493896	22.608005098504165
82-83	20.281253865203286	28.700548035253355	29.368551492310434	21.649646607232924
84-85	19.24804769906495	28.147542202721798	29.101546620757524	23.502863477455726
86	20.06607402463943	28.161452118102503	29.2654986704664	22.50697518679167
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	661.0
1	843.0
2	2950.5
3	3436.0
4	2204.0
5	2730.0
6	3234.5
7	3421.0
8	3540.0
9	3802.5
10	3981.0
11	4040.0
12	4223.5
13	4482.0
14	4581.0
15	4534.5
16	4849.5
17	5276.0
18	5658.5
19	6208.5
20	7077.5
21	7714.0
22	7960.0
23	10134.5
24	12944.0
25	14696.5
26	18355.5
27	23865.0
28	29660.5
29	32734.0
30	36822.0
31	44974.5
32	52025.0
33	57480.0
34	61697.5
35	69622.5
36	75799.0
37	78081.5
38	84605.0
39	92229.5
40	101761.5
41	110536.5
42	117827.0
43	122491.0
44	127241.0
45	132233.0
46	135917.0
47	141661.5
48	142996.0
49	132634.0
50	123240.0
51	113312.0
52	98840.5
53	88799.5
54	78001.5
55	68655.0
56	59448.5
57	54288.0
58	47107.5
59	34377.0
60	25411.5
61	20358.0
62	16153.0
63	11461.0
64	9336.0
65	7647.0
66	4855.0
67	3151.5
68	2020.5
69	1386.0
70	1092.0
71	902.0
72	766.0
73	460.5
74	232.0
75	137.0
76	83.5
77	59.0
78	34.5
79	17.0
80	18.0
81	13.5
82	7.5
83	5.0
84	2.5
85	4.0
86	6.0
87	4.5
88	2.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2595271.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.8086458716617
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.69906235105051	13.396333153622935
2	7.1404095419184435	2.400412307374825
3	2.258705614231865	1.1389734839387262
4	1.1220882677108737	0.7544313731479362
5	0.6939356468207026	0.5832059272565848
6	0.5103675855058536	0.5147152805485748
7	0.4114916668191603	0.4841632394692149
8	0.3268214843289551	0.43947412746689923
9	0.27281170456220344	0.41270357984674255
>10	4.0876582528877385	17.14686103822994
>50	1.8863840270846495	23.115390517125196
>100	1.585404203385361	38.56964530501626
>500	0.003471181209739502	0.3765252555208631
>1k	0.0011570604032465008	0.2674185053415221
>5k	0.0	0.0
>10k+	2.3141208064930013E-4	0.3997469060937924
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10277	0.39598947470225654	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.706324310640392E-5	0.0	0.0	0.022540998608623145	0.0
2	7.706324310640392E-5	0.0	0.0	0.08018430445221328	0.0
3	7.706324310640392E-5	0.0	0.0	0.12152873437879898	0.0
4	7.706324310640392E-5	0.0	0.0	0.18129127940781523	0.0
5	7.706324310640392E-5	0.0	0.0	0.3248601013150457	0.0
6	7.706324310640392E-5	0.0	0.0	0.44950989703965405	0.0
7	7.706324310640392E-5	0.0	0.0	0.5280758733866329	0.0
8	7.706324310640392E-5	0.0	0.0	0.6840518774339943	0.0
9	7.706324310640392E-5	0.0	0.0	0.7378420211222643	0.0
10	7.706324310640392E-5	0.0	0.0	0.8595248819872761	0.0
11	7.706324310640392E-5	0.0	0.0	1.0484839540841784	0.0
12	7.706324310640392E-5	0.0	0.0	1.1946343946354736	0.0
13	7.706324310640392E-5	0.0	0.0	1.2469603367047217	0.0
14	7.706324310640392E-5	0.0	0.0	1.2716976377418774	0.0
15	7.706324310640392E-5	0.0	0.0	1.3093045003778025	0.0
16	7.706324310640392E-5	0.0	0.0	1.3919162969878676	0.0
17	7.706324310640392E-5	0.0	0.0	1.4998819005799395	0.0
18	1.1559486465960587E-4	0.0	0.0	1.621680356309611	0.0
19	1.1559486465960587E-4	0.0	0.0	1.7085691629120812	0.0
20	1.1559486465960587E-4	0.0	0.0	1.807749556790023	0.0
21	1.1559486465960587E-4	0.0	0.0	1.9318213781913334	0.0
22	1.1559486465960587E-4	0.0	0.0	2.071267316592371	0.0
23	1.1559486465960587E-4	0.0	0.0	2.2148361384996016	0.0
24	1.1559486465960587E-4	0.0	0.0	2.321337540472652	0.0
25	1.1559486465960587E-4	0.0	0.0	2.4157014816564435	0.0
26	1.1559486465960587E-4	0.0	0.0	2.5068672982513194	3.853162155320196E-5
27	1.1559486465960587E-4	0.0	0.0	2.612521004550199	3.853162155320196E-5
28	1.1559486465960587E-4	0.0	0.0	2.7228755686785697	3.853162155320196E-5
29	1.1559486465960587E-4	0.0	0.0	2.8463694157565818	3.853162155320196E-5
30	1.1559486465960587E-4	0.0	0.0	2.984158494430832	3.853162155320196E-5
31	1.5412648621280784E-4	0.0	0.0	3.1067275825915677	3.853162155320196E-5
32	1.5412648621280784E-4	0.0	0.0	3.2281021904841536	3.853162155320196E-5
33	1.5412648621280784E-4	0.0	0.0	3.348282318108591	3.853162155320196E-5
34	1.5412648621280784E-4	0.0	0.0	3.4765540862592	3.853162155320196E-5
35	1.5412648621280784E-4	0.0	0.0	3.6175027579008128	3.853162155320196E-5
36	1.5412648621280784E-4	0.0	0.0	3.75706429116651	3.853162155320196E-5
37	1.5412648621280784E-4	0.0	0.0	3.906605514414487	3.853162155320196E-5
38	1.5412648621280784E-4	0.0	0.0	4.062620050083401	3.853162155320196E-5
39	1.5412648621280784E-4	0.0	0.0	4.2822117613151	3.853162155320196E-5
40	1.5412648621280784E-4	0.0	0.0	4.445123457242038	3.853162155320196E-5
41	1.5412648621280784E-4	0.0	0.0	4.614855250183892	3.853162155320196E-5
42	1.5412648621280784E-4	0.0	0.0	4.784317321774874	3.853162155320196E-5
43	1.5412648621280784E-4	0.0	0.0	4.9437611717620245	3.853162155320196E-5
44	1.5412648621280784E-4	0.0	0.0	5.1001224920249175	3.853162155320196E-5
45	1.5412648621280784E-4	0.0	0.0	5.258525988230131	3.853162155320196E-5
46	1.5412648621280784E-4	0.0	0.0	5.436310890076605	3.853162155320196E-5
47	1.5412648621280784E-4	0.0	0.0	5.6162920943516115	3.853162155320196E-5
48	1.926581077660098E-4	0.0	0.0	5.798777854027575	3.853162155320196E-5
49	1.926581077660098E-4	0.0	0.0	5.981533335054412	3.853162155320196E-5
50	1.926581077660098E-4	0.0	0.0	6.169529116612485	3.853162155320196E-5
51	1.926581077660098E-4	0.0	0.0	6.364730311401006	3.853162155320196E-5
52	1.926581077660098E-4	0.0	0.0	6.579312911830788	3.853162155320196E-5
53	1.926581077660098E-4	0.0	0.0	6.77019856500535	3.853162155320196E-5
54	1.926581077660098E-4	0.0	0.0	6.968829074112106	3.853162155320196E-5
55	1.926581077660098E-4	0.0	0.0	7.166766014030904	3.853162155320196E-5
56	1.926581077660098E-4	0.0	0.0	7.366822193135129	3.853162155320196E-5
57	1.926581077660098E-4	0.0	0.0	7.573428747903398	3.853162155320196E-5
58	1.926581077660098E-4	0.0	0.0	7.776028014030134	3.853162155320196E-5
59	1.926581077660098E-4	0.0	0.0	7.9911885887832135	3.853162155320196E-5
60	1.926581077660098E-4	0.0	0.0	8.199028155441185	3.853162155320196E-5
61	1.926581077660098E-4	0.0	0.0	8.430526137732823	3.853162155320196E-5
62	1.926581077660098E-4	0.0	0.0	8.68922744484102	3.853162155320196E-5
63	1.926581077660098E-4	0.0	0.0	8.931051901708916	3.853162155320196E-5
64	1.926581077660098E-4	0.0	0.0	9.174957066140685	3.853162155320196E-5
65	1.926581077660098E-4	0.0	0.0	9.400020267632938	3.853162155320196E-5
66	2.3118972931921174E-4	0.0	0.0	9.629707263711573	3.853162155320196E-5
67	2.3118972931921174E-4	0.0	0.0	9.860742866544573	3.853162155320196E-5
68	2.3118972931921174E-4	0.0	0.0	10.128961484176411	3.853162155320196E-5
69	2.3118972931921174E-4	0.0	0.0	10.45590229305533	3.853162155320196E-5
70	2.697213508724137E-4	0.0	0.0	10.702389076131164	3.853162155320196E-5
71	2.697213508724137E-4	0.0	0.0	10.946679556778463	3.853162155320196E-5
72	2.697213508724137E-4	3.853162155320196E-5	0.0	11.202336865783959	3.853162155320196E-5
73	2.697213508724137E-4	3.853162155320196E-5	0.0	11.459766629380901	3.853162155320196E-5
74	2.697213508724137E-4	3.853162155320196E-5	0.0	11.731645751060293	3.853162155320196E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	8000	0.0	70.8	1
CGAGGGA	1920	0.0	66.041664	4
TACGGGA	1310	0.0	65.64885	4
TAACGCG	45	9.429641E-9	62.222225	1
CGTAAGG	450	0.0	62.22222	2
AGGGATT	5345	0.0	61.739944	6
ACGGGAT	1335	0.0	61.72285	5
GCGGGAT	2110	0.0	61.23223	5
CCGATCC	1775	0.0	61.070423	80
GCGGGTC	450	0.0	60.444443	5
CGGGATA	795	0.0	60.377357	6
TAGGGAC	3650	0.0	60.054794	5
CGAACGG	60	2.1827873E-11	59.999996	2
GCGATAC	120	0.0	59.999996	9
GGGCGAT	1750	0.0	59.885715	7
TAGGGTA	2285	0.0	59.5186	5
TAGGGAT	5535	0.0	59.331528	5
TAAGGGA	4045	0.0	59.23362	4
AAGGGAT	5335	0.0	59.231487	5
ATAGGGA	4195	0.0	58.92729	4
>>END_MODULE