FastQCFastQC Report
Thu 26 May 2016
SRR1547835_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547835_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2540484
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115010.452709011353742No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTCG900.075.555561
TATAGCG3300.075.151511
CGAACGG750.074.6666642
CGTTTTT67000.070.507461
TAACGCG1100.069.0909041
ATAGGGA38400.068.3333364
ACGGGAT14850.068.148155
CGTAAGG4850.067.628872
AGGGATT53600.066.194036
TACGGGA13450.066.02234
TAGGGAC35750.065.790215
TAGCGGG18700.065.668453
CCGTAAG800.065.000011
TAGGGAT44600.063.85655
AACGGGA10500.063.619054
TTAGCGG7500.062.9333342
AGTACGG3250.062.769232
TAGGGTA21750.062.160925
CGGGATT17700.061.9209026
CGGGATA7950.061.886796
Produced by FastQC (version 0.11.5)