##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547835_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2540484
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.047029621127315	33.0	31.0	34.0	31.0	34.0
2	32.47781210194593	34.0	31.0	34.0	31.0	34.0
3	32.60331968239123	34.0	31.0	34.0	31.0	34.0
4	36.12177836979095	37.0	35.0	37.0	35.0	37.0
5	36.08231226805601	37.0	35.0	37.0	35.0	37.0
6	36.13980682421145	37.0	36.0	37.0	35.0	37.0
7	36.352113612996575	37.0	37.0	37.0	35.0	37.0
8	36.355105562562095	37.0	37.0	37.0	35.0	37.0
9	38.14390368134576	39.0	39.0	39.0	37.0	39.0
10-11	37.735227814857325	39.0	37.5	39.0	35.0	39.0
12-13	37.12969280656756	39.0	35.0	39.0	34.0	39.0
14-15	38.29796999311942	40.0	37.0	41.0	33.5	41.0
16-17	38.45502254688476	40.0	37.0	41.0	34.0	41.0
18-19	38.36770316207463	40.0	36.0	41.0	34.0	41.0
20-21	38.221101569622164	40.0	35.5	41.0	34.0	41.0
22-23	38.021373092686275	40.0	35.0	41.0	34.0	41.0
24-25	38.01167257892591	40.0	35.0	41.0	34.0	41.0
26-27	38.01755452897952	40.0	35.0	41.0	34.0	41.0
28-29	37.94455328197304	40.0	36.0	41.0	34.0	41.0
30-31	37.818901437678804	40.0	35.5	41.0	34.0	41.0
32-33	37.55965949795393	40.0	35.0	41.0	33.0	41.0
34-35	37.324320483813324	40.0	35.0	41.0	33.0	41.0
36-37	37.158248585702566	40.0	35.0	41.0	32.5	41.0
38-39	37.06896599230698	40.0	35.0	41.0	32.0	41.0
40-41	36.96208379977988	40.0	35.0	41.0	32.0	41.0
42-43	36.91669894398075	40.0	35.0	41.0	32.0	41.0
44-45	36.79317051396505	39.0	35.0	41.0	31.5	41.0
46-47	36.68282835081819	39.0	35.0	41.0	31.0	41.0
48-49	36.590827180962364	39.0	35.0	41.0	31.0	41.0
50-51	36.37813404847265	39.0	35.0	40.5	31.0	41.0
52-53	36.20283930148743	38.5	35.0	40.0	31.0	41.0
54-55	36.04571746958454	38.0	35.0	40.0	30.5	41.0
56-57	35.75413818784138	38.0	35.0	40.0	29.5	41.0
58-59	35.508401351868386	37.0	35.0	40.0	29.0	41.0
60-61	35.139212252468425	36.5	34.5	40.0	28.5	41.0
62-63	34.73214001741401	36.0	34.0	39.5	27.5	41.0
64-65	34.2882257868973	35.5	34.0	39.0	26.5	40.5
66-67	33.9266899535679	35.0	34.0	38.5	26.0	40.0
68-69	33.42224709937162	35.0	33.5	38.0	24.5	40.0
70-71	33.078155973428686	35.0	33.0	37.0	24.0	39.5
72-73	32.76542560394003	35.0	33.0	37.0	23.0	39.0
74-75	32.42428745861025	35.0	33.0	36.0	22.5	39.0
76-77	30.45619673259111	33.0	30.0	34.5	19.5	36.0
78-79	31.43711473876631	34.5	31.5	35.0	20.0	37.0
80-81	31.5310745905111	35.0	32.0	35.0	19.5	37.0
82-83	31.28813663065778	35.0	32.0	35.0	18.5	36.0
84-85	31.026377847685715	34.0	32.0	35.0	17.5	36.0
86	30.608883582813355	34.0	31.0	35.0	15.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	16.0
10	21.0
11	16.0
12	38.0
13	84.0
14	228.0
15	751.0
16	1825.0
17	4096.0
18	8769.0
19	14374.0
20	17511.0
21	17536.0
22	15654.0
23	14283.0
24	14920.0
25	17080.0
26	20597.0
27	25510.0
28	32071.0
29	40982.0
30	53032.0
31	66699.0
32	81498.0
33	103573.0
34	242410.0
35	232088.0
36	211644.0
37	388829.0
38	603564.0
39	310018.0
40	760.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.255074229949884	24.16067174601375	30.483010324016995	13.101243700019365
2	30.416212028888985	25.6348790230523	30.82011144333127	13.128797504727446
3	29.223250372763616	25.121748454231557	32.12179254031909	13.533208632685739
4	26.920893813934665	26.85283591630571	31.553869262707423	14.6724010070522
5	23.969487703917835	31.530999604799714	29.794519469518406	14.704993221764044
6	22.064299558666775	41.22521535266508	26.149584094999224	10.560900993668923
7	88.73568973471197	3.4876818747923624	6.0703393526587845	1.7062890378368847
8	89.30798225849877	2.6138720023428608	5.924501000596736	2.1536447385616286
9	84.23875135604082	5.9956685418998905	7.0562932102701685	2.709286891789124
10-11	35.84226470231657	30.672915082322895	20.977518457112897	12.50730175824764
12-13	28.02780887421452	25.814470785881742	29.193708757858737	16.964011582044996
14-15	16.91902015521452	32.37721630996299	34.58598833922984	16.11777519559265
16-17	21.71743651997021	27.468525682507742	31.37843025187326	19.435607545648782
18-19	23.417624358193162	28.87003027769512	29.419827088066686	18.292518276045037
20-21	25.10511776496132	28.01192607392922	29.716246982858387	17.166709178251075
22-23	21.408046655676635	24.75437751231655	31.925530725641256	21.912045106365557
24-25	19.29028090710274	28.45853388566903	33.357206736983976	18.893978470244253
26-27	18.34611042620225	33.10790384824309	29.52882206697621	19.017163658578447
28-29	17.06235898356376	28.235387430111743	34.5817962246564	20.12045736166809
30-31	21.476694991977908	28.354262416138027	30.51351632208666	19.655526269797406
32-33	23.142066629823294	28.19309233988484	29.257672947359637	19.407168082932227
34-35	18.757350961470333	28.38455192002784	30.571792619044245	22.286304499457586
36-37	21.768430739969233	28.43761267537997	30.220639059328853	19.573317525321947
38-39	18.87423813729982	30.50271129438327	28.700672785185816	21.92237778313109
40-41	20.385347831358118	27.022626397174708	29.770409890398835	22.821615881068332
42-43	20.690880162992563	26.498060999400114	29.560036591452654	23.25102224615467
44-45	19.368179449270293	30.13443501317072	28.697386009909927	21.79999952764906
46-47	20.41788100220273	29.147930079465173	28.793017393536037	21.641171524796064
48-49	20.306642356338397	27.100544620631347	30.909562902187144	21.683250120843116
50-51	18.66455368347134	29.530770514594856	29.740120386509027	22.064555415424778
52-53	20.774781498328664	26.319532026180838	30.81269159734917	22.09299487814133
54-55	20.657795916053793	26.59627063189534	30.988465190097635	21.757468261953232
56-57	20.91022812975795	25.718701633232094	30.534968927180806	22.83610130982915
58-59	21.994765564357028	27.369666567472972	29.054365231192165	21.581202636977835
60-61	21.995080464982266	29.416343499900023	27.808382182292824	20.780193852824894
62-63	21.942964411505837	27.057757498177516	29.821404110397857	21.177873979918786
64-65	19.415768806258964	30.537704626362533	29.288651296367146	20.75787527101135
66-67	18.612693486752917	29.359779475092147	26.643033374742764	25.384493663412165
68-69	18.26193355281907	31.28311376887239	27.187240699016407	23.267711979292134
70-71	18.787286202156757	29.603473196446036	29.44472391874934	22.16451668264787
72-73	21.164667834947988	29.474206489786987	28.111414990214463	21.249710685050566
74-75	19.903057842521346	31.12442353504293	26.089123174954064	22.88339544748166
76-77	22.655820701882003	29.22216790186437	27.193853612146345	20.928157784107277
78-79	18.534204505913046	29.770744472313147	29.494871843318045	22.20017917845576
80-81	17.004141730473407	29.21260279537285	29.75877824855421	24.02447722559953
82-83	18.600491087525054	28.432515221508975	30.235951102230914	22.731042588735058
84-85	16.94606224640659	28.00310885642264	29.701466334761406	25.349362562409368
86	18.10076347656588	28.124876991943264	30.35205102649731	23.422308504993534
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	878.0
1	1135.0
2	3731.0
3	4051.5
4	2233.0
5	2620.5
6	3087.5
7	3367.0
8	3489.5
9	3729.5
10	3696.5
11	3739.0
12	4043.0
13	4136.5
14	4119.0
15	4215.0
16	4299.5
17	4394.0
18	4635.0
19	5122.0
20	6381.5
21	7289.0
22	7166.5
23	8604.0
24	11401.5
25	15298.5
26	18604.5
27	20921.0
28	26792.0
29	30993.0
30	38776.5
31	44996.0
32	48249.0
33	54759.5
34	61527.5
35	71006.0
36	75410.0
37	77022.5
38	83603.0
39	92659.0
40	106489.0
41	120536.5
42	129767.0
43	134692.0
44	139464.0
45	144210.5
46	141941.0
47	140555.5
48	140372.0
49	128772.5
50	118215.0
51	104931.5
52	87146.0
53	77136.5
54	66037.0
55	58407.0
56	51945.0
57	47521.0
58	42269.0
59	31381.5
60	22321.5
61	16724.5
62	12024.5
63	8017.5
64	6538.0
65	5104.0
66	3544.5
67	3269.0
68	2877.0
69	1994.0
70	1120.0
71	887.0
72	618.0
73	244.5
74	140.5
75	128.5
76	118.5
77	131.0
78	92.5
79	44.0
80	32.5
81	17.5
82	14.0
83	9.5
84	5.0
85	3.5
86	2.0
87	2.0
88	1.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2540484.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.809359402706956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.79930228367353	13.855557356497819
2	9.51466745655159	3.3890026466193395
3	3.2341850249122435	1.7279629045054472
4	1.6190114069740473	1.1533422409553227
5	0.9207806094646634	0.8199256402499874
6	0.6316301148745123	0.6749356635223963
7	0.42772626232448774	0.5332271512199322
8	0.36809283770929674	0.524439811226172
9	0.2665315102698555	0.42720799126879233
>10	2.667484719727473	10.718379961216339
>50	0.9504572760451879	12.415709134093285
>100	1.5918778033180023	52.033112604544804
>500	0.005576144699395014	0.6688563120104309
>1k	0.0024535036677338063	0.6014860598920267
>5k	0.0	0.0
>10k+	2.2304578797580055E-4	0.4568545221779289
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11501	0.452709011353742	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.872515630879786E-5	0.0	0.0	0.06813662278526454	0.0
2	7.872515630879786E-5	0.0	0.0	0.23302646267404165	0.0
3	7.872515630879786E-5	0.0	0.0	0.33899052306568356	0.0
4	7.872515630879786E-5	0.0	0.0	0.5705605703480124	0.0
5	7.872515630879786E-5	0.0	0.0	1.0782197408052954	0.0
6	7.872515630879786E-5	0.0	0.0	1.465390059531963	0.0
7	7.872515630879786E-5	0.0	0.0	1.7086507925261485	0.0
8	7.872515630879786E-5	0.0	0.0	2.1925349657781745	0.0
9	7.872515630879786E-5	0.0	0.0	2.328532673301623	0.0
10	7.872515630879786E-5	0.0	0.0	2.6202093774257187	0.0
11	7.872515630879786E-5	0.0	0.0	3.0872070046495077	0.0
12	7.872515630879786E-5	0.0	0.0	3.4789433824420857	0.0
13	7.872515630879786E-5	0.0	0.0	3.6046280944890814	0.0
14	7.872515630879786E-5	0.0	0.0	3.6580824756227552	0.0
15	7.872515630879786E-5	0.0	0.0	3.746372738423072	0.0
16	7.872515630879786E-5	0.0	0.0	3.9373206050500613	0.0
17	7.872515630879786E-5	0.0	0.0	4.191563497349324	0.0
18	7.872515630879786E-5	0.0	0.0	4.505204520083574	0.0
19	7.872515630879786E-5	0.0	0.0	4.699183305228453	0.0
20	7.872515630879786E-5	0.0	0.0	4.9044591503036425	0.0
21	7.872515630879786E-5	0.0	0.0	5.171770418550166	0.0
22	7.872515630879786E-5	0.0	0.0	5.449158506804215	0.0
23	7.872515630879786E-5	0.0	0.0	5.738040467879349	0.0
24	7.872515630879786E-5	0.0	0.0	5.94851217327092	0.0
25	7.872515630879786E-5	0.0	0.0	6.139263227007137	0.0
26	7.872515630879786E-5	0.0	0.0	6.321630051596467	0.0
27	7.872515630879786E-5	0.0	0.0	6.542217939573719	0.0
28	7.872515630879786E-5	0.0	0.0	6.749028925196931	0.0
29	7.872515630879786E-5	0.0	0.0	6.995911015381321	0.0
30	7.872515630879786E-5	0.0	0.0	7.253735902292634	0.0
31	7.872515630879786E-5	0.0	0.0	7.495697670207724	0.0
32	7.872515630879786E-5	0.0	0.0	7.70451614731681	0.0
33	7.872515630879786E-5	0.0	0.0	7.9113271329400225	0.0
34	7.872515630879786E-5	0.0	0.0	8.129513903649856	0.0
35	7.872515630879786E-5	0.0	0.0	8.381276953525392	0.0
36	7.872515630879786E-5	0.0	0.0	8.6063915379904	0.0
37	7.872515630879786E-5	0.0	0.0	8.838985012304741	0.0
38	7.872515630879786E-5	0.0	0.0	9.103462174924148	0.0
39	7.872515630879786E-5	0.0	0.0	9.521689567814636	0.0
40	7.872515630879786E-5	0.0	0.0	9.79482649762801	0.0
41	1.1808773446319677E-4	0.0	0.0	10.040212809842535	0.0
42	1.1808773446319677E-4	0.0	0.0	10.305595311759491	0.0
43	1.1808773446319677E-4	0.0	0.0	10.540550540763098	0.0
44	1.1808773446319677E-4	0.0	0.0	10.7768047348458	0.0
45	1.1808773446319677E-4	0.0	0.0	11.01463343205468	0.0
46	1.1808773446319677E-4	0.0	0.0	11.26305066278709	0.0
47	1.1808773446319677E-4	0.0	0.0	11.544965447528895	0.0
48	1.1808773446319677E-4	0.0	0.0	11.802908422174673	0.0
49	1.1808773446319677E-4	0.0	0.0	12.073644234720629	0.0
50	1.1808773446319677E-4	0.0	0.0	12.34201829257732	0.0
51	1.1808773446319677E-4	0.0	0.0	12.601653858083735	0.0
52	1.1808773446319677E-4	0.0	0.0	12.915294880817985	0.0
53	1.1808773446319677E-4	0.0	0.0	13.179968856328165	0.0
54	1.9681289077199462E-4	0.0	0.0	13.457002681378825	0.0
55	1.9681289077199462E-4	0.0	0.0	13.724628850250582	0.0
56	1.9681289077199462E-4	0.0	0.0	14.008826664525342	0.0
57	1.9681289077199462E-4	0.0	0.0	14.289993560282214	0.0
58	2.3617546892639355E-4	0.0	0.0	14.560847460562632	0.0
59	2.3617546892639355E-4	0.0	0.0	14.836385507643426	0.0
60	2.3617546892639355E-4	0.0	0.0	15.141366763183708	0.0
61	2.755380470807925E-4	0.0	0.0	15.442451123486705	0.0
62	2.755380470807925E-4	0.0	0.0	15.79147910398176	0.0
63	2.755380470807925E-4	0.0	0.0	16.11763742656911	0.0
64	2.755380470807925E-4	0.0	0.0	16.435135981962492	0.0
65	2.755380470807925E-4	0.0	0.0	16.729922329760786	0.0
66	2.755380470807925E-4	0.0	0.0	17.024393776933845	0.0
67	2.755380470807925E-4	0.0	0.0	17.327918617082414	0.0
68	2.755380470807925E-4	0.0	0.0	17.65120347146449	0.0
69	2.755380470807925E-4	0.0	0.0	18.076988479360626	0.0
70	3.1490062523519143E-4	0.0	0.0	18.39059013951672	3.936257815439893E-5
71	3.1490062523519143E-4	0.0	0.0	18.693052190055123	3.936257815439893E-5
72	3.1490062523519143E-4	0.0	0.0	18.992916310435334	3.936257815439893E-5
73	3.1490062523519143E-4	0.0	0.0	19.30356577722985	3.936257815439893E-5
74	3.5426320338959033E-4	0.0	0.0	19.638029603807777	3.936257815439893E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTCG	90	0.0	75.55556	1
TATAGCG	330	0.0	75.15151	1
CGAACGG	75	0.0	74.666664	2
CGTTTTT	6700	0.0	70.50746	1
TAACGCG	110	0.0	69.090904	1
ATAGGGA	3840	0.0	68.333336	4
ACGGGAT	1485	0.0	68.14815	5
CGTAAGG	485	0.0	67.62887	2
AGGGATT	5360	0.0	66.19403	6
TACGGGA	1345	0.0	66.0223	4
TAGGGAC	3575	0.0	65.79021	5
TAGCGGG	1870	0.0	65.66845	3
CCGTAAG	80	0.0	65.00001	1
TAGGGAT	4460	0.0	63.8565	5
AACGGGA	1050	0.0	63.61905	4
TTAGCGG	750	0.0	62.933334	2
AGTACGG	325	0.0	62.76923	2
TAGGGTA	2175	0.0	62.16092	5
CGGGATT	1770	0.0	61.920902	6
CGGGATA	795	0.0	61.88679	6
>>END_MODULE