FastQCFastQC Report
Thu 26 May 2016
SRR1547834_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547834_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1603263
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110310.6880343399679278No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCC30740.19173398250941986No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA21890.13653405585983086No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTT17730.11058697169460033No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG1250.080.02
GCGAACG700.080.01
ATACGGT700.074.285713
CGAACGG1300.073.846152
CGTTTTT56100.073.4402851
CTGCGCG1100.072.727271
AGTACGG2050.072.195122
ACGGGAT10700.069.5327155
GTTAGCG750.069.3333361
TATAGCG1100.069.0909041
CGGGGTA2150.068.8372046
TGTTCGG351.1817792E-768.571432
CGTAACG351.1817792E-768.5714380
CGTAAGG3450.068.40582
AATACGG2700.068.148152
TAGCGGG11450.067.0742343
TAGGGAC16550.066.948655
CACTACG600.066.6666641
CCGATCC10650.066.47887480
CTAAGCG850.065.8823551
Produced by FastQC (version 0.11.5)