##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547834_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1603263
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03098930119388	33.0	31.0	34.0	31.0	34.0
2	32.45315646902598	34.0	31.0	34.0	31.0	34.0
3	32.563547590133375	34.0	31.0	34.0	31.0	34.0
4	36.0783396111555	37.0	35.0	37.0	35.0	37.0
5	36.046480833150895	37.0	35.0	37.0	35.0	37.0
6	36.103434059165586	37.0	35.0	37.0	35.0	37.0
7	36.33729338230845	37.0	37.0	37.0	35.0	37.0
8	36.3340337798602	37.0	37.0	37.0	35.0	37.0
9	38.12728791221403	39.0	39.0	39.0	37.0	39.0
10-11	37.66790227180444	39.0	37.5	39.0	35.0	39.0
12-13	36.964284711865744	39.0	35.0	39.0	33.5	39.0
14-15	38.159681225101565	40.0	36.0	41.0	33.5	41.0
16-17	38.32022288295806	40.0	36.0	41.0	34.0	41.0
18-19	38.20359572945924	40.0	36.0	41.0	34.0	41.0
20-21	37.992084580009646	40.0	35.0	41.0	34.0	41.0
22-23	37.80968655797583	40.0	35.0	41.0	34.0	41.0
24-25	37.80317296663118	40.0	35.0	41.0	34.0	41.0
26-27	37.7721509197181	40.0	35.0	41.0	34.0	41.0
28-29	37.70642059350213	40.0	35.0	41.0	34.0	41.0
30-31	37.56964609050418	40.0	35.0	41.0	33.5	41.0
32-33	37.21150460030575	40.0	35.0	41.0	33.0	41.0
34-35	36.937694252284246	40.0	35.0	41.0	32.0	41.0
36-37	36.74124675739414	39.5	35.0	41.0	31.5	41.0
38-39	36.66729133024339	39.0	35.0	41.0	31.0	41.0
40-41	36.57762513074898	39.0	35.0	41.0	31.0	41.0
42-43	36.53569907120666	39.0	35.0	41.0	31.0	41.0
44-45	36.383559341168606	39.0	35.0	41.0	31.0	41.0
46-47	36.22233158252888	39.0	35.0	41.0	30.0	41.0
48-49	36.131453791424114	38.0	35.0	41.0	30.0	41.0
50-51	35.91883801971355	38.0	35.0	40.0	30.0	41.0
52-53	35.73581221546309	38.0	35.0	40.0	29.0	41.0
54-55	35.57099646159114	37.5	35.0	40.0	29.0	41.0
56-57	35.29475326256515	37.0	34.5	40.0	28.5	41.0
58-59	34.992257976389396	36.5	34.0	40.0	28.0	41.0
60-61	34.60549236151523	36.0	34.0	39.0	26.5	41.0
62-63	34.10555286312976	35.0	33.5	39.0	26.0	40.5
64-65	33.68242016437728	35.0	33.0	38.5	25.0	40.0
66-67	33.313798484715235	35.0	33.0	38.0	24.0	40.0
68-69	32.842474378813705	35.0	33.0	37.0	22.0	39.5
70-71	32.47719213878197	35.0	33.0	36.5	20.5	39.0
72-73	32.16218486923231	35.0	33.0	36.0	20.0	39.0
74-75	31.843134906749547	35.0	32.0	36.0	19.0	38.0
76-77	29.906908286413397	33.0	29.5	34.5	16.0	36.0
78-79	30.83118989211377	34.0	30.5	35.0	17.0	36.5
80-81	30.997065671695786	34.5	31.5	35.0	16.0	36.0
82-83	30.78643397870468	34.5	31.0	35.0	14.0	36.0
84-85	30.557485265985683	34.0	31.0	35.0	11.0	36.0
86	30.16275183797044	34.0	30.0	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	5.0
10	7.0
11	4.0
12	14.0
13	47.0
14	188.0
15	500.0
16	1350.0
17	3222.0
18	7121.0
19	11288.0
20	13267.0
21	13562.0
22	12066.0
23	11078.0
24	11042.0
25	12808.0
26	14902.0
27	18473.0
28	22579.0
29	28956.0
30	36400.0
31	45792.0
32	53481.0
33	67222.0
34	169102.0
35	160748.0
36	127431.0
37	242415.0
38	357694.0
39	160193.0
40	303.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.70683786752392	24.069163948771973	30.538782470499225	13.685215713204881
2	29.249973335628653	26.75113191036031	30.720723923648208	13.278170830362829
3	29.064850869757485	25.010681341738696	33.028080857601026	12.89638693090279
4	25.63653000162793	27.584058261183596	33.17072744771132	13.608684289477146
5	24.551305680976856	31.936744002699495	29.956657142340337	13.555293173983307
6	22.47017488708964	41.48265131796842	25.50136814733453	10.545805647607411
7	87.77624132784203	3.6073307997502595	7.062160107231315	1.5542677651763934
8	88.4808044594056	3.769001093395157	6.062012283698932	1.6881821635003114
9	84.31461338532729	5.977060532177191	7.023551344975839	2.6847747375196707
10-11	39.21873703815282	29.675480566819047	20.311733009493764	10.794049385534375
12-13	28.814236965488504	27.3528734836393	27.474344508667635	16.358545042204554
14-15	15.402744278387265	34.053240173321534	33.94645794233385	16.59755760595735
16-17	19.107220711760952	27.973825878848324	32.74144666221325	20.177506747177475
18-19	22.638020087783474	29.874512166749934	29.658982961622645	17.828484783843948
20-21	25.663475050568746	29.41516769238734	28.41467681846335	16.50668043858057
22-23	20.52311442352253	26.950756051876706	30.39523147481106	22.13089804978971
24-25	18.903199287952134	29.584790517837682	33.33445604370587	18.177554150504317
26-27	18.348424431924144	35.853880492470665	27.956143190480915	17.841551885124275
28-29	16.81071664474263	29.051004108496237	35.65905905643678	18.47922019032436
30-31	22.257202966699786	30.837735293585645	28.755325857329705	18.149735882384864
32-33	24.31347820039507	29.75790622000258	27.773796314141848	18.1548192654605
34-35	20.28594185732472	28.727070979620933	29.77964314027081	21.207344022783538
36-37	21.91203189994405	30.59392002435034	29.356880312213278	18.13716776349233
38-39	19.514702204192325	31.726984281431058	28.61773146389582	20.140582050480802
40-41	20.079113657584564	28.486249604712388	28.881100605452755	22.553536132250294
42-43	21.349242139312143	28.14469616026815	27.117728033391902	23.388333667027805
44-45	19.43732874768519	32.35227158613403	27.684603212323864	20.525796453856916
46-47	21.216855874550838	30.52047605414707	27.728919085639724	20.533748985662363
48-49	20.263456463474803	28.00064618219219	30.969747321556103	20.766150032776906
50-51	18.543869595942773	31.02466657061256	29.21959778277176	21.21186605067291
52-53	21.84254860244389	27.11648057742242	30.04313702742469	20.997833792708995
54-55	20.828117408060937	28.290180712709017	31.07646717974531	19.80523469948474
56-57	22.010923971924758	26.39379814790212	29.20665542708838	22.388622453084743
58-59	23.07503510029234	27.89807411510151	28.437567635503346	20.589323149102796
60-61	23.29720700845713	30.49792828749868	26.69643096609851	19.508433737945676
62-63	22.651617357850835	27.752589562660653	30.292784153317324	19.303008926171188
64-65	19.705407035526925	32.69295181139963	29.035473281676182	18.56616787139727
66-67	18.52674826276163	30.722906971594803	25.905325576652118	24.84501918899145
68-69	17.529594333556002	34.394294635377975	25.289363005321025	22.786748025744995
70-71	19.07035838786275	31.28404385306715	28.842523029596517	20.80307472947358
72-73	22.093973353093034	32.00968899051497	26.214445165889817	19.681892490502182
74-75	20.478798550206673	33.04096084048594	25.08253480558087	21.397705803726524
76-77	24.107679151829736	30.461564945988272	26.614909718492846	18.81584618368914
78-79	18.861565444970662	30.79061264433845	28.89120499880556	21.456616911885323
80-81	16.723862522867428	30.325841736508607	29.656051440094355	23.294244300529606
82-83	18.959958534563576	28.8096837511999	30.210264940936078	22.02009277330045
84-85	17.167083628824468	29.235222168789527	28.261114988620083	25.336579213765926
86	18.421431792538094	29.187662909953016	30.336569857846158	22.054335439662736
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	785.0
1	965.0
2	3962.5
3	4394.0
4	2119.0
5	2549.0
6	3220.5
7	3573.0
8	3768.0
9	3785.0
10	3535.5
11	3434.0
12	3243.0
13	3149.0
14	3216.0
15	3218.5
16	3249.0
17	3442.0
18	3724.0
19	3839.0
20	4328.5
21	4820.0
22	5004.0
23	5275.5
24	6912.0
25	8216.0
26	9638.0
27	13531.0
28	18538.0
29	21319.0
30	25917.0
31	31545.0
32	30732.0
33	30133.5
34	34813.5
35	42291.0
36	46333.0
37	49473.5
38	56912.5
39	62522.5
40	67945.5
41	78163.5
42	88551.0
43	92832.0
44	92219.0
45	90996.0
46	92860.0
47	95309.0
48	93740.5
49	81319.0
50	70441.0
51	59736.5
52	46311.5
53	40892.5
54	35247.5
55	31299.5
56	29264.0
57	28230.0
58	24538.0
59	17669.0
60	12264.5
61	9514.0
62	8685.0
63	6623.5
64	4868.0
65	3567.5
66	1786.0
67	1042.0
68	709.0
69	498.5
70	307.0
71	256.0
72	198.0
73	102.0
74	47.5
75	25.0
76	15.0
77	9.5
78	8.5
79	9.0
80	7.0
81	5.5
82	3.5
83	1.0
84	2.0
85	3.0
86	3.0
87	1.5
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1603263.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.78559809027814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.99299841805303	14.65145122137243
2	9.409413405832051	3.535229170144722
3	3.203531540962313	1.805407679941422
4	1.5139205703300727	1.1375961349930162
5	0.8914106233090038	0.8372840851443634
6	0.5804851033870456	0.6542855909773556
7	0.45124224314608524	0.5933798794768547
8	0.32631163495295684	0.4903967381126242
9	0.25755829833620225	0.4354548009623863
>10	2.8830950874068395	12.860871754177818
>50	1.0216208231616257	13.893308942287206
>100	1.4573513964577796	46.51079483763452
>500	0.008044257938129289	1.076199179836412
>1k	0.002681419312709763	0.8237721664989338
>5k	0.0	0.0
>10k+	3.351774140887204E-4	0.6945678184399402
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11031	0.6880343399679278	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCC	3074	0.19173398250941986	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	2189	0.13653405585983086	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTT	1773	0.11058697169460033	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06455584642070578	0.0
2	0.0	0.0	0.0	0.2583481312797713	0.0
3	0.0	0.0	0.0	0.3728645892782407	0.0
4	0.0	0.0	0.0	0.6067002107576861	0.0
5	0.0	0.0	0.0	1.228931248335426	0.0
6	0.0	0.0	0.0	1.6065361702976992	0.0
7	0.0	0.0	0.0	1.9051771293917468	0.0
8	0.0	0.0	0.0	2.4544319927547757	0.0
9	0.0	0.0	0.0	2.604126709092644	0.0
10	0.0	0.0	0.0	3.1156460293788357	0.0
11	0.0	0.0	0.0	3.6481226099523285	0.0
12	0.0	0.0	0.0	4.107747761908059	0.0
13	0.0	0.0	0.0	4.2498953696305595	0.0
14	0.0	0.0	0.0	4.30359834911677	0.0
15	0.0	0.0	0.0	4.40021381395317	0.0
16	0.0	0.0	0.0	4.604297610560463	0.0
17	0.0	0.0	0.0	4.8693820040754385	0.0
18	0.0	0.0	0.0	5.168896182348123	0.0
19	0.0	0.0	0.0	5.392377919280866	0.0
20	0.0	0.0	0.0	5.592282738390395	0.0
21	0.0	0.0	0.0	5.838281055572292	0.0
22	0.0	0.0	0.0	6.141974211342743	0.0
23	0.0	0.0	0.0	6.433691789806164	0.0
24	0.0	0.0	0.0	6.646882014990679	0.0
25	0.0	0.0	0.0	6.83743091432909	0.0
26	0.0	0.0	0.0	7.012386614048974	0.0
27	0.0	0.0	0.0	7.194140948802536	0.0
28	0.0	0.0	0.0	7.387247132878386	0.0
29	0.0	0.0	0.0	7.603244133994235	0.0
30	0.0	0.0	0.0	7.858535998148775	0.0
31	0.0	0.0	0.0	8.08145637989525	0.0
32	0.0	0.0	0.0	8.27786832229023	0.0
33	0.0	0.0	0.0	8.482575846882265	0.0
34	0.0	0.0	0.0	8.709425714932609	0.0
35	0.0	0.0	0.0	8.965653171064261	0.0
36	0.0	0.0	0.0	9.208470475523978	0.0
37	0.0	0.0	0.0	9.44498812733781	0.0
38	0.0	0.0	0.0	9.666286816323959	0.0
39	0.0	0.0	0.0	9.917274957383786	0.0
40	0.0	0.0	0.0	10.169198690420723	0.0
41	0.0	0.0	0.0	10.433596983152484	0.0
42	6.237279847411185E-5	0.0	0.0	10.695500363945278	0.0
43	6.237279847411185E-5	0.0	0.0	10.940625461948539	0.0
44	6.237279847411185E-5	0.0	0.0	11.175708539397466	0.0
45	6.237279847411185E-5	0.0	0.0	11.423016685347319	0.0
46	6.237279847411185E-5	0.0	0.0	11.702758686503712	0.0
47	1.247455969482237E-4	0.0	0.0	11.974953579044735	0.0
48	1.247455969482237E-4	0.0	0.0	12.232366118347395	0.0
49	1.247455969482237E-4	0.0	0.0	12.499883051002861	0.0
50	1.247455969482237E-4	0.0	0.0	12.79216198465255	0.0
51	1.247455969482237E-4	0.0	0.0	13.068348736295917	0.0
52	1.247455969482237E-4	0.0	0.0	13.356760556440209	0.0
53	1.247455969482237E-4	0.0	0.0	13.62889307618276	0.0
54	1.247455969482237E-4	0.0	0.0	13.918178115505691	0.0
55	1.247455969482237E-4	0.0	0.0	14.207213663634725	0.0
56	1.247455969482237E-4	0.0	0.0	14.518142064028172	0.0
57	1.247455969482237E-4	0.0	0.0	14.807427103351102	0.0
58	1.247455969482237E-4	0.0	0.0	15.091971809990001	0.0
59	1.247455969482237E-4	0.0	0.0	15.37832532778465	0.0
60	1.8711839542233556E-4	0.0	0.0	15.673161546171777	0.0
61	1.8711839542233556E-4	0.0	0.0	15.99693874305089	0.0
62	1.8711839542233556E-4	0.0	0.0	16.33356473641567	0.0
63	1.8711839542233556E-4	0.0	0.0	16.705992716104593	0.0
64	1.8711839542233556E-4	0.0	0.0	17.037753631188394	0.0
65	1.8711839542233556E-4	0.0	0.0	17.332652222373994	0.0
66	1.8711839542233556E-4	0.0	0.0	17.62717657676875	0.0
67	1.8711839542233556E-4	0.0	0.0	17.923759233513152	0.0
68	1.8711839542233556E-4	0.0	0.0	18.262194038033684	0.0
69	1.8711839542233556E-4	0.0	0.0	18.610795608705498	0.0
70	1.8711839542233556E-4	0.0	0.0	18.912180970932404	0.0
71	2.494911938964474E-4	0.0	0.0	19.214938534725743	0.0
72	2.494911938964474E-4	0.0	0.0	19.520814738442787	0.0
73	2.494911938964474E-4	0.0	0.0	19.834612287566046	0.0
74	2.494911938964474E-4	0.0	0.0	20.17716369678587	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	125	0.0	80.0	2
GCGAACG	70	0.0	80.0	1
ATACGGT	70	0.0	74.28571	3
CGAACGG	130	0.0	73.84615	2
CGTTTTT	5610	0.0	73.440285	1
CTGCGCG	110	0.0	72.72727	1
AGTACGG	205	0.0	72.19512	2
ACGGGAT	1070	0.0	69.532715	5
GTTAGCG	75	0.0	69.333336	1
TATAGCG	110	0.0	69.090904	1
CGGGGTA	215	0.0	68.837204	6
TGTTCGG	35	1.1817792E-7	68.57143	2
CGTAACG	35	1.1817792E-7	68.57143	80
CGTAAGG	345	0.0	68.4058	2
AATACGG	270	0.0	68.14815	2
TAGCGGG	1145	0.0	67.074234	3
TAGGGAC	1655	0.0	66.94865	5
CACTACG	60	0.0	66.666664	1
CCGATCC	1065	0.0	66.478874	80
CTAAGCG	85	0.0	65.882355	1
>>END_MODULE