FastQCFastQC Report
Thu 26 May 2016
SRR1547833_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547833_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences926804
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59850.6457676056641966No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA204.0904808E-580.000019
GATCGCG251.2846085E-680.01
TAATACG351.2896635E-979.999991
CGTAAGG1450.074.482762
CGTTTTT33500.073.074621
TGCGTAG550.072.727271
CGTGCGG1050.072.380962
TCACGGG1500.072.03
TACGGGT1400.071.4285664
CTAACGG850.070.5882342
CGAAACG1000.068.080
TTTACGG1250.067.22
AGGGTAA4550.066.813196
CCTAACG303.795005E-666.6666641
TCGTGCG600.066.6666641
ACTACGG303.795005E-666.6666642
CGCGAGG1100.065.4545442
TAGTGCG559.094947E-1265.4545441
TAGGGCG1300.064.615395
TTCCGCG251.2354009E-464.01
Produced by FastQC (version 0.11.5)