##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547833_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	926804
Sequences flagged as poor quality	0
Sequence length	86
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11917298587404	33.0	31.0	34.0	31.0	34.0
2	32.53969555591042	34.0	31.0	34.0	31.0	34.0
3	32.65441668357064	34.0	31.0	34.0	31.0	34.0
4	36.16141924290357	37.0	35.0	37.0	35.0	37.0
5	36.13453437835832	37.0	35.0	37.0	35.0	37.0
6	36.18510170435173	37.0	37.0	37.0	35.0	37.0
7	36.36027898023746	37.0	37.0	37.0	35.0	37.0
8	36.35875222808706	37.0	37.0	37.0	35.0	37.0
9	38.16895697472173	39.0	39.0	39.0	37.0	39.0
10-11	37.78248583303481	39.0	37.5	39.0	35.0	39.0
12-13	36.87354985520132	39.0	35.0	39.0	33.5	39.0
14-15	37.985706794532604	40.0	35.0	41.0	33.0	41.0
16-17	38.16285805844601	40.0	35.0	41.0	34.0	41.0
18-19	38.002919711179494	40.0	36.0	41.0	34.0	41.0
20-21	37.80909070310443	40.0	35.0	41.0	34.0	41.0
22-23	37.61036583786863	39.5	35.0	41.0	34.0	41.0
24-25	37.59868213775512	39.0	35.0	41.0	34.0	41.0
26-27	37.592447809892924	39.0	35.0	41.0	34.0	41.0
28-29	37.549068627239414	39.0	35.0	41.0	34.0	41.0
30-31	37.39660057574201	39.0	35.0	41.0	33.5	41.0
32-33	37.04440744752936	39.0	35.0	41.0	33.0	41.0
34-35	36.713863988502425	39.0	35.0	41.0	32.0	41.0
36-37	36.52954346334285	39.0	35.0	41.0	31.5	41.0
38-39	36.46182148544892	39.0	35.0	41.0	31.0	41.0
40-41	36.34663531879448	39.0	35.0	41.0	31.0	41.0
42-43	36.30704766056253	38.5	35.0	41.0	31.0	41.0
44-45	36.151665832257954	38.0	35.0	41.0	31.0	41.0
46-47	36.03031601072071	38.0	35.0	41.0	31.0	41.0
48-49	35.96598957276835	38.0	35.0	40.0	31.0	41.0
50-51	35.74273093340123	37.5	35.0	40.0	30.0	41.0
52-53	35.574941951048984	37.0	35.0	40.0	30.0	41.0
54-55	35.41301181263784	37.0	35.0	40.0	29.5	41.0
56-57	35.1056890129952	36.0	34.5	40.0	28.5	41.0
58-59	34.86673665629411	36.0	34.0	39.5	28.0	41.0
60-61	34.55448778814075	35.0	34.0	39.0	27.5	41.0
62-63	34.13288678080802	35.0	33.5	39.0	26.5	40.0
64-65	33.868217012442756	35.0	34.0	38.0	26.0	40.0
66-67	33.621292635767645	35.0	34.0	37.5	26.0	40.0
68-69	33.27709148859953	35.0	33.5	37.0	25.5	39.5
70-71	33.03997555038606	35.0	33.0	36.5	25.0	39.0
72-73	32.81677085985818	35.0	33.0	36.0	25.0	39.0
74-75	32.53847793060884	35.0	33.0	35.5	25.0	38.0
76-77	30.597932788378124	33.0	30.0	34.5	20.5	36.0
78-79	31.593325557507306	34.0	31.5	35.0	22.5	36.5
80-81	31.87115614520438	35.0	33.0	35.0	23.0	36.0
82-83	31.71379007859267	35.0	32.5	35.0	22.5	36.0
84-85	31.520610075053625	35.0	32.0	35.0	20.5	36.0
86	31.145997427719344	34.0	32.0	35.0	20.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	7.0
10	10.0
11	12.0
12	17.0
13	54.0
14	93.0
15	320.0
16	853.0
17	1850.0
18	4111.0
19	6590.0
20	8068.0
21	8592.0
22	7285.0
23	6114.0
24	5515.0
25	6014.0
26	6898.0
27	8417.0
28	10492.0
29	12926.0
30	16709.0
31	21208.0
32	27050.0
33	38661.0
34	116943.0
35	106827.0
36	77898.0
37	139854.0
38	197151.0
39	90021.0
40	240.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.71511560157271	24.249247953181037	29.079395427727977	13.956241017518266
2	30.607658145627337	25.373649660553905	30.392725970108025	13.625966223710732
3	29.57928537209593	24.423934294629717	33.1223214401319	12.874458893142455
4	26.407417318008985	26.113827734882456	32.503851947121504	14.974902999987053
5	23.15764714006413	30.65934113361617	31.252238876828326	14.930772849491369
6	21.698115243352422	39.60945356299714	27.803073789064353	10.889357404586084
7	86.57903936538901	3.9411785016033596	7.411167841312727	2.0686142916949
8	87.8269839146141	3.1758602681904695	7.146710631374055	1.8504451858213817
9	83.57969969918128	5.289252096451893	8.68446834497909	2.4465798593877452
10-11	43.01114367223275	24.78636259662237	19.129503109611093	13.072990621533787
12-13	30.07966085601702	27.75090526152239	26.3626937302817	15.806740152178886
14-15	16.022265764929802	31.505690523562695	35.865781761839614	16.606261949667893
16-17	20.78082312980954	24.08448819815193	33.906144125402996	21.228544546635533
18-19	25.477015636531565	27.414318453524157	29.496959443420618	17.611706466523668
20-21	27.866841317042223	26.707318915326216	28.360311349541	17.065528418090555
22-23	22.223199295643955	24.941789202463518	30.453850004963297	22.381161496929234
24-25	20.86126084911157	26.341006296908514	34.208311573968174	18.589421280011738
26-27	18.08856025653752	33.57241660588431	29.902600765641928	18.436422371936246
28-29	16.906271444663595	28.656112835076243	36.412930889378984	18.02468483088118
30-31	24.1916845417154	28.0629453476679	30.48082442458168	17.264545686035017
32-33	26.939622617079774	27.491357395954267	27.809439752094296	17.759580234871667
34-35	20.59928528577779	28.34078186973729	30.12362915999499	20.936303684489925
36-37	23.63946422328777	29.452829292924932	29.00127750851313	17.906428975274167
38-39	20.69196939158657	31.610944708913642	27.520166076106705	20.17691982339308
40-41	21.369404965882755	27.094347888010837	28.589755762815006	22.9464913832914
42-43	22.105428979590076	25.955164198687104	27.380924122036593	24.558482699686234
44-45	19.804295190784675	32.10829905783747	27.651693346165963	20.435712405211888
46-47	22.36664925917454	30.012656397684946	27.223771153339865	20.39692318980065
48-49	21.959767113650784	26.4650886271531	31.002671546519007	20.572472712677115
50-51	19.497326295527426	31.30003754839211	28.742862568568974	20.45977358751149
52-53	22.864057556937606	26.013374996223583	29.528735309731076	21.59383213710774
54-55	22.297648693790702	26.813436282104952	30.582464037703765	20.306450986400577
56-57	23.69638024868257	24.88811010742293	29.05652111989158	22.358988524002918
58-59	24.7617619259304	26.988770009624474	28.00489639664913	20.244571667795995
60-61	25.824230365859446	29.268216365056688	25.80712858382139	19.10042468526247
62-63	24.353962650139618	25.4509583471802	30.239133624800928	19.95594537787925
64-65	20.80590934005464	29.945436143995924	29.874601318078042	19.374053197871394
66-67	19.07970833099555	29.268324262735163	25.937091337542782	25.714876068726504
68-69	18.647470231030507	32.56788921929556	25.188443295454054	23.596197254219877
70-71	20.18625297258104	29.616564020008546	28.973979395859317	21.223203611551096
72-73	23.67409937807778	30.15081937497033	25.85298509717265	20.32209614977924
74-75	21.95248402035382	32.14746591512337	25.12899167461513	20.771058389907683
76-77	26.960716613221347	27.39565215514823	26.081404482501153	19.562226749129266
78-79	19.342708922274827	29.7783026400404	29.331228609285244	21.54775982839953
80-81	17.79297456635923	29.08689431638189	29.81412466929362	23.306006447965267
82-83	20.17109334875551	26.768928489734616	31.090608154474946	21.96937000703493
84-85	17.85582496407007	26.790400127750853	29.55187936176365	25.80189554641542
86	19.474883578404928	26.821960198704364	30.96641792655189	22.736738296338814
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	597.0
1	694.0
2	2193.5
3	2368.5
4	1182.0
5	1378.5
6	1645.0
7	1756.0
8	1819.0
9	1873.0
10	1876.0
11	1883.0
12	1966.0
13	2067.5
14	2081.0
15	2198.0
16	2318.0
17	2325.0
18	2576.5
19	2527.0
20	2586.0
21	2942.0
22	2732.0
23	3115.0
24	3999.0
25	5069.5
26	6153.5
27	7224.0
28	8748.5
29	9507.0
30	11391.0
31	14265.5
32	15891.0
33	16371.0
34	16745.5
35	18626.0
36	19977.0
37	21008.5
38	24546.5
39	28380.0
40	31874.5
41	38869.5
42	44095.5
43	44494.0
44	46040.0
45	49551.0
46	54652.5
47	58045.0
48	54908.0
49	49129.5
50	46744.0
51	41760.0
52	33592.5
53	27645.0
54	24192.5
55	24618.0
56	23503.5
57	21275.0
58	20139.5
59	16683.5
60	13239.5
61	10447.5
62	7546.5
63	5495.0
64	4676.0
65	3778.5
66	2367.5
67	1487.5
68	1017.5
69	752.0
70	542.0
71	494.0
72	361.5
73	141.0
74	60.0
75	70.0
76	64.0
77	38.5
78	17.5
79	13.0
80	11.0
81	7.5
82	4.5
83	4.5
84	4.0
85	1.5
86	1.0
87	1.0
88	0.5
89	0.5
90	0.5
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	926804.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.667758123577965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.01165431674316	14.312943136591288
2	6.97501129875296	2.464656250711814
3	2.150074822569163	1.1396100573824035
4	1.0751489270591803	0.7598188476042387
5	0.6379596348201128	0.5635658260303938
6	0.49641337916175987	0.5262306907402782
7	0.3648201537139311	0.4511887964096987
8	0.2702502794082801	0.3819773255531884
9	0.2607410816889403	0.41460393277442525
>10	3.4929747736063215	15.372744166377656
>50	1.6703542753584981	21.576087370764117
>100	1.590285670713732	40.89931769387808
>500	0.003695474060527651	0.4859807551951691
>1k	0.0	0.0
>5k	6.159123434212751E-4	0.6512751499872564
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5985	0.6457676056641966	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03323248496985339	0.0
2	0.0	0.0	0.0	0.12257176274595276	0.0
3	0.0	0.0	0.0	0.1742547507347832	0.0
4	0.0	0.0	0.0	0.26424141458172384	0.0
5	0.0	0.0	0.0	0.4427041747769755	0.0
6	0.0	0.0	0.0	0.5923582548197893	0.0
7	0.0	0.0	0.0	0.6772737277784732	0.0
8	0.0	0.0	0.0	0.8651235860009237	0.0
9	0.0	0.0	0.0	0.9268410580877942	0.0
10	0.0	0.0	0.0	1.0591236118963665	0.0
11	0.0	0.0	0.0	1.2676898243857384	0.0
12	0.0	0.0	0.0	1.4081726017583005	0.0
13	0.0	0.0	0.0	1.463416213136758	0.0
14	0.0	0.0	0.0	1.4851036465099416	0.0
15	0.0	0.0	0.0	1.5214651641555281	0.0
16	0.0	0.0	0.0	1.6088622837191036	0.0
17	0.0	0.0	0.0	1.7100703061272933	0.0
18	0.0	0.0	0.0	1.8267076965572009	0.0
19	0.0	0.0	0.0	1.9048256157720511	0.0
20	0.0	0.0	0.0	1.9840225117716368	0.0
21	0.0	0.0	0.0	2.098825641667494	0.0
22	0.0	0.0	0.0	2.22150530209192	0.0
23	0.0	0.0	0.0	2.352169390723389	0.0
24	0.0	0.0	0.0	2.4473351431370602	0.0
25	0.0	0.0	0.0	2.5405587373382073	0.0
26	0.0	0.0	0.0	2.6296822197573597	0.0
27	0.0	0.0	0.0	2.7242005861001894	0.0
28	0.0	0.0	0.0	2.828861334219533	0.0
29	0.0	0.0	0.0	2.943232873401496	0.0
30	0.0	0.0	0.0	3.0696889525724966	0.0
31	1.0789767847354997E-4	0.0	0.0	3.1970082131712854	0.0
32	1.0789767847354997E-4	0.0	0.0	3.3173141246692937	0.0
33	1.0789767847354997E-4	0.0	0.0	3.4379437292027224	0.0
34	1.0789767847354997E-4	0.0	0.0	3.5611628780195166	0.0
35	1.0789767847354997E-4	0.0	0.0	3.702724632176814	0.0
36	1.0789767847354997E-4	0.0	0.0	3.8327413347374417	0.0
37	1.0789767847354997E-4	0.0	0.0	3.9682608189002204	0.0
38	1.0789767847354997E-4	0.0	0.0	4.118778080370823	0.0
39	1.0789767847354997E-4	0.0	0.0	4.327236395181721	0.0
40	1.0789767847354997E-4	0.0	0.0	4.494909387529618	0.0
41	1.0789767847354997E-4	0.0	0.0	4.666898287016457	0.0
42	1.0789767847354997E-4	0.0	0.0	4.832305428116409	0.0
43	1.0789767847354997E-4	0.0	0.0	4.968364400671555	0.0
44	1.0789767847354997E-4	0.0	0.0	5.120392229640787	0.0
45	1.0789767847354997E-4	0.0	0.0	5.272204263253072	0.0
46	1.0789767847354997E-4	0.0	0.0	5.440740437028757	0.0
47	1.0789767847354997E-4	0.0	0.0	5.605068601343974	0.0
48	1.0789767847354997E-4	0.0	0.0	5.76087284905978	0.0
49	1.0789767847354997E-4	0.0	0.0	5.938256632470296	0.0
50	1.0789767847354997E-4	0.0	0.0	6.12103529980449	0.0
51	1.0789767847354997E-4	0.0	0.0	6.292808403934381	0.0
52	1.0789767847354997E-4	0.0	0.0	6.494361267322972	0.0
53	1.0789767847354997E-4	0.0	0.0	6.686203339648944	0.0
54	1.0789767847354997E-4	0.0	0.0	6.880419160901334	0.0
55	1.0789767847354997E-4	0.0	0.0	7.066111065554313	0.0
56	1.0789767847354997E-4	0.0	0.0	7.260866375199071	0.0
57	1.0789767847354997E-4	0.0	0.0	7.453463731274358	0.0
58	1.0789767847354997E-4	0.0	0.0	7.63225018450503	0.0
59	1.0789767847354997E-4	0.0	0.0	7.826358108078947	0.0
60	1.0789767847354997E-4	0.0	0.0	8.029421538966167	0.0
61	1.0789767847354997E-4	0.0	0.0	8.240253602703485	0.0
62	1.0789767847354997E-4	0.0	0.0	8.490144626048226	0.0
63	1.0789767847354997E-4	0.0	0.0	8.737554002788077	0.0
64	1.0789767847354997E-4	0.0	0.0	8.954104643484492	0.0
65	1.0789767847354997E-4	0.0	0.0	9.160836595439813	0.0
66	1.0789767847354997E-4	0.0	0.0	9.352894463122732	0.0
67	1.0789767847354997E-4	0.0	0.0	9.566316071143413	0.0
68	1.0789767847354997E-4	0.0	0.0	9.805956815033168	0.0
69	1.0789767847354997E-4	0.0	0.0	10.069658741222524	0.0
70	1.0789767847354997E-4	0.0	0.0	10.297754433515609	0.0
71	1.0789767847354997E-4	0.0	0.0	10.525310637416325	0.0
72	1.0789767847354997E-4	0.0	0.0	10.765814562733869	0.0
73	1.0789767847354997E-4	0.0	0.0	11.017431948934187	0.0
74	1.0789767847354997E-4	0.0	0.0	11.283076033336068	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	20	4.0904808E-5	80.00001	9
GATCGCG	25	1.2846085E-6	80.0	1
TAATACG	35	1.2896635E-9	79.99999	1
CGTAAGG	145	0.0	74.48276	2
CGTTTTT	3350	0.0	73.07462	1
TGCGTAG	55	0.0	72.72727	1
CGTGCGG	105	0.0	72.38096	2
TCACGGG	150	0.0	72.0	3
TACGGGT	140	0.0	71.428566	4
CTAACGG	85	0.0	70.588234	2
CGAAACG	100	0.0	68.0	80
TTTACGG	125	0.0	67.2	2
AGGGTAA	455	0.0	66.81319	6
CCTAACG	30	3.795005E-6	66.666664	1
TCGTGCG	60	0.0	66.666664	1
ACTACGG	30	3.795005E-6	66.666664	2
CGCGAGG	110	0.0	65.454544	2
TAGTGCG	55	9.094947E-12	65.454544	1
TAGGGCG	130	0.0	64.61539	5
TTCCGCG	25	1.2354009E-4	64.0	1
>>END_MODULE