FastQCFastQC Report
Thu 26 May 2016
SRR1547832_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547832_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2198022
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85650.38966852925038964No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGGGT5050.070.495054
CGAGGGA14400.066.944444
CGTTTTT56750.066.678421
CCGCGAC303.7967366E-666.6666645
TAGGGAC21800.066.422025
CGGGATT7900.066.329126
TACGGGA8400.066.1904754
CGTTAGG2850.064.561412
GCGGGAT14600.064.383565
CCGATCC17300.064.0462580
TCGCACG251.2357763E-464.01
TAGGGAT38100.062.3622065
CGAAACG2850.061.75438780
GGGCGAT16550.061.6314167
GTACGCG1050.060.9523851
ATAGGGA28300.060.3533554
CGGGATC5700.060.350886
TAGGGTA16650.060.30035
CGAAGGG15200.060.2631573
TTAGGGA27750.060.108114
Produced by FastQC (version 0.11.5)