FastQCFastQC Report
Thu 26 May 2016
SRR1547831_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547831_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1966441
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97390.49526021884206034No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA20400.10374071736706059No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54400.071.98531
CGTAAGG4650.067.096772
GACGGGT3050.065.573774
TACGGGA11350.065.198244
CCGATCC14200.064.78873480
GCGGGAT15850.064.353325
TAAGGGA29450.064.2444844
CGGGATT12250.063.346946
GCGTAAG2150.063.255811
CTACGGG5750.062.6086963
TAGGGTA17700.062.5988665
CGGGTAT2600.061.538466
TAGGGAC24700.061.2145735
TAGGGCG3350.060.8955235
GGACGTA1250.060.88
TGCGGGA19000.060.421054
AGGGATT45900.060.392166
CGGGATG11200.060.357146
CGTAGGG12050.060.0829853
TAGGGAT42050.060.023785
Produced by FastQC (version 0.11.5)