##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547831_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1966441
Sequences flagged as poor quality	0
Sequence length	86
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03776721498382	33.0	31.0	34.0	31.0	34.0
2	32.475462523411586	34.0	31.0	34.0	31.0	34.0
3	32.61328969442765	34.0	31.0	34.0	31.0	34.0
4	36.133197995770026	37.0	35.0	37.0	35.0	37.0
5	36.088639832062086	37.0	35.0	37.0	35.0	37.0
6	36.144297743995374	37.0	36.0	37.0	35.0	37.0
7	36.34318192104416	37.0	37.0	37.0	35.0	37.0
8	36.34262812868528	37.0	37.0	37.0	35.0	37.0
9	38.13859810693532	39.0	39.0	39.0	37.0	39.0
10-11	37.72984417025479	39.0	37.5	39.0	35.0	39.0
12-13	37.13788895776685	39.0	37.0	39.0	34.0	39.0
14-15	38.34281120053945	40.0	37.0	41.0	33.5	41.0
16-17	38.48154839123066	40.0	37.0	41.0	34.0	41.0
18-19	38.367146281022414	40.0	37.0	41.0	34.0	41.0
20-21	38.18683932037625	40.0	35.5	41.0	34.0	41.0
22-23	38.0026982757174	40.0	35.0	41.0	34.0	41.0
24-25	37.994990696390076	40.0	35.0	41.0	34.0	41.0
26-27	37.991820502115246	40.0	35.0	41.0	34.0	41.0
28-29	37.923333830000495	40.0	36.0	41.0	34.0	41.0
30-31	37.7868489316486	40.0	35.5	41.0	33.5	41.0
32-33	37.51063367779659	40.0	35.0	41.0	33.0	41.0
34-35	37.25501655020415	40.0	35.0	41.0	33.0	41.0
36-37	37.08805501919458	40.0	35.0	41.0	32.0	41.0
38-39	36.99089192098822	40.0	35.0	41.0	32.0	41.0
40-41	36.892185425344564	40.0	35.0	41.0	31.5	41.0
42-43	36.8466526582796	40.0	35.0	41.0	31.5	41.0
44-45	36.73739690130545	39.0	35.0	41.0	31.0	41.0
46-47	36.611564242202036	39.0	35.0	41.0	31.0	41.0
48-49	36.50970509666956	39.0	35.0	41.0	31.0	41.0
50-51	36.28107632011334	39.0	35.0	40.5	31.0	41.0
52-53	36.09069684775694	38.0	35.0	40.0	30.0	41.0
54-55	35.91359415309181	38.0	35.0	40.0	30.0	41.0
56-57	35.628142415663625	37.5	35.0	40.0	29.5	41.0
58-59	35.37420522659973	37.0	35.0	40.0	29.0	41.0
60-61	35.03399135799142	36.5	34.5	40.0	28.5	41.0
62-63	34.660570289167076	36.0	34.0	39.0	27.5	41.0
64-65	34.30710430671452	35.0	34.0	39.0	27.0	40.0
66-67	34.024196759526475	35.0	34.0	38.5	26.5	40.0
68-69	33.56173640602489	35.0	33.5	37.5	25.5	40.0
70-71	33.27354647304445	35.0	33.0	37.0	26.0	39.0
72-73	32.98388891403302	35.0	33.0	36.5	25.0	39.0
74-75	32.665841741501524	35.0	33.0	36.0	25.0	38.5
76-77	30.70240627610999	33.0	30.0	34.5	21.0	36.0
78-79	31.742922111571104	34.5	32.0	35.0	23.0	37.0
80-81	31.857084194237203	35.0	32.5	35.0	23.0	36.5
82-83	31.65483556333498	35.0	32.0	35.0	21.0	36.0
84-85	31.422231584878467	34.5	32.0	35.0	20.0	36.0
86	30.996594354979376	34.0	31.0	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	6.0
11	10.0
12	21.0
13	50.0
14	185.0
15	607.0
16	1519.0
17	3489.0
18	7489.0
19	11841.0
20	14560.0
21	14786.0
22	13383.0
23	11829.0
24	11872.0
25	12946.0
26	15338.0
27	18483.0
28	22628.0
29	28693.0
30	35932.0
31	44767.0
32	56577.0
33	76324.0
34	184867.0
35	180606.0
36	170570.0
37	326726.0
38	479379.0
39	220545.0
40	407.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.05684787898544	24.732498966406823	30.424965712167314	12.78568744244043
2	29.860545015080543	26.13543960891784	31.150794760686946	12.853220615314672
3	29.327805919424993	24.79642155549035	32.01977582851456	13.855996696570097
4	26.56779430453291	26.951431545619727	32.43168750041318	14.04908664943418
5	24.510015810288742	31.666040323610012	29.600125302513526	14.22381856358772
6	21.996286692557774	41.49913473122255	25.912397066578656	10.592181509641021
7	88.74479325848068	3.5033850494370284	6.324318909135846	1.42750278294645
8	89.61082483532432	3.5899882071213933	5.257874505261027	1.5413124522932546
9	85.0517762800918	5.879454303485332	6.47530233553918	2.5934670808836877
10-11	37.04446255951742	29.98213523822988	20.60244878946279	12.370953412789907
12-13	28.94589769029429	25.18402535341767	28.256860999134986	17.61321595715305
14-15	18.097669851269373	31.325399541608416	32.86528810170252	17.71164250541969
16-17	23.152054905283197	27.018430758919287	30.88536599877647	18.944148337021044
18-19	24.576048811024588	28.363576634132425	28.506169267219306	18.55420528762368
20-21	26.950719599520152	27.32355051588123	28.56747291172224	17.15825697287638
22-23	22.613467680952542	24.274793904317495	31.534965961348448	21.576772453381516
24-25	20.51594733836408	28.471741587975437	32.021098014128064	18.991213059532424
26-27	19.714194323653746	32.45645305401993	28.686240777119682	19.143111845206644
28-29	18.253865740187475	28.488192628204967	33.64883563758079	19.60910599402677
30-31	22.717157545026776	28.34382012986914	29.82761750797507	19.111404817129017
32-33	25.045450130464125	28.029826473308887	28.08563796218651	18.83908543404048
34-35	20.62622270385941	28.002619961646445	30.34555829541797	21.02559903907618
36-37	22.57029323534243	29.15930353364276	29.322949429960016	18.9474538010548
38-39	20.687017815434075	30.20896635088467	28.221492533973812	20.882523299707444
40-41	21.39667551683473	26.932081867699054	29.059987052751644	22.611255562714568
42-43	21.17131914967192	27.294716698848326	28.46042673032143	23.073537421158328
44-45	20.882523299707444	29.87671636219953	28.19209933071981	21.04866100737322
46-47	21.29804555539678	29.682990743175107	28.122150626436287	20.896813074991822
48-49	21.64008480295112	27.224437448161424	30.21377198705682	20.921705761830637
50-51	19.929863138533015	29.251983659819945	29.112493077595513	21.705660124051523
52-53	21.853592352885236	26.60062010505273	29.628857412960773	21.916930129101257
54-55	21.712220198826206	27.04856133491928	30.077841135330274	21.161377330924243
56-57	21.895876865870882	26.840647647196125	29.278961331664664	21.984514155268325
58-59	22.458797390819253	27.45137026740187	28.839436321760985	21.250396020017888
60-61	23.75133553460287	28.787921936127248	27.26616257492597	20.194579954343915
62-63	22.935216464668912	26.826179885386846	29.554433618908476	20.684170031035766
64-65	21.2344026594238	29.553416553051935	29.093499372724636	20.11868141479963
66-67	20.63809694773451	29.300828247580274	26.56138678963671	23.499688015048505
68-69	19.6905475424892	31.01087192547348	26.794422004016393	22.504158528020927
70-71	20.592430690775874	29.38542270019797	28.428007756144225	21.594138852881933
72-73	22.4622808413779	29.499817182412286	27.51480975020354	20.523092226006273
74-75	21.275898946370624	30.901028812967184	26.101545889248644	21.721526351413544
76-77	23.540141809492376	28.985410698820864	26.730296001761555	20.7441514899252
78-79	20.098187537790356	29.323839362584486	28.684308351992254	21.893664747632908
80-81	18.83359327841517	29.137970577301836	28.338836507172093	23.689599637110902
82-83	20.230965485361626	28.702666390702795	29.224904281389573	21.841463842546
84-85	19.012825708983897	27.834397268974765	28.740323254041183	24.41245376800016
86	19.749689922046986	28.70271724399562	29.330094317602207	22.21749851635518
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	588.0
1	821.5
2	3261.0
3	3647.0
4	1967.0
5	2310.5
6	2652.5
7	2791.0
8	2887.5
9	3022.0
10	3100.0
11	3130.5
12	3153.0
13	3300.5
14	3416.0
15	3535.5
16	3652.5
17	3796.5
18	3934.5
19	4256.5
20	4634.0
21	4681.0
22	4827.5
23	5880.0
24	7726.0
25	10183.0
26	12277.0
27	15216.0
28	20755.5
29	23933.0
30	25615.0
31	29643.5
32	34632.5
33	37893.0
34	43236.0
35	50544.0
36	53127.0
37	54890.0
38	60352.5
39	64912.0
40	71904.5
41	83049.5
42	91051.0
43	94040.0
44	98406.0
45	104009.5
46	107526.5
47	111450.5
48	111839.0
49	105221.5
50	99860.0
51	92800.0
52	76762.5
53	65158.5
54	60120.0
55	54970.5
56	48403.5
57	44574.0
58	39555.0
59	29535.5
60	21754.5
61	17246.0
62	13509.0
63	9278.5
64	7057.0
65	5915.0
66	4012.0
67	2602.5
68	1578.5
69	1071.5
70	766.5
71	593.0
72	441.5
73	206.5
74	93.5
75	62.0
76	55.5
77	51.0
78	40.0
79	29.0
80	19.5
81	9.0
82	8.0
83	5.5
84	3.0
85	3.0
86	3.0
87	2.0
88	1.0
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1966441.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.255189068024915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.21693526148483	14.461201305878953
2	7.9117298751175085	2.888602494508225
3	2.5552552024271042	1.3994000051208317
4	1.2221731528818789	0.8924400791889128
5	0.7716294976913798	0.7043121185410616
6	0.5520957727685638	0.6047167629328472
7	0.4138053175939715	0.5287866017022433
8	0.32880432711543167	0.48019081263015334
9	0.297087987640017	0.48810576457868027
>10	3.7656623483301654	16.80257455576844
>50	1.515280367145619	20.020021407047984
>100	1.4439225142619394	39.364104235681076
>500	0.00421378165623025	0.5633355589147029
>1k	0.0011236751083280665	0.30277042693057854
>5k	2.8091877708201664E-4	0.4994378705753565
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9739	0.49526021884206034	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2040	0.10374071736706059	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.085329282699049E-5	0.0	0.0	0.03371573314429469	0.0
2	5.085329282699049E-5	0.0	0.0	0.1101482322632614	0.0
3	5.085329282699049E-5	0.0	0.0	0.15978104606240412	0.0
4	5.085329282699049E-5	0.0	0.0	0.24450263191217025	0.0
5	5.085329282699049E-5	0.0	0.0	0.4633243509467103	0.0
6	5.085329282699049E-5	0.0	0.0	0.6096801277027889	0.0
7	5.085329282699049E-5	0.0	0.0	0.7119969528706938	0.0
8	1.0170658565398098E-4	0.0	0.0	0.9075787170832992	0.0
9	1.0170658565398098E-4	0.0	0.0	0.96789072237611	0.0
10	1.0170658565398098E-4	0.0	0.0	1.1793895672435635	0.0
11	1.0170658565398098E-4	0.0	0.0	1.4494205521548829	0.0
12	1.0170658565398098E-4	0.0	0.0	1.7059245611742229	0.0
13	1.0170658565398098E-4	0.0	0.0	1.7935956380079545	0.0
14	1.0170658565398098E-4	0.0	0.0	1.8300574489649066	0.0
15	1.0170658565398098E-4	0.0	0.0	1.897234648789361	0.0
16	1.0170658565398098E-4	0.0	0.0	2.0279276113547264	0.0
17	1.0170658565398098E-4	0.0	0.0	2.2038800045361135	0.0
18	1.0170658565398098E-4	0.0	0.0	2.395698625079522	0.0
19	1.0170658565398098E-4	0.0	0.0	2.5436817072060642	0.0
20	1.5255987848097145E-4	0.0	0.0	2.688257618713198	0.0
21	1.5255987848097145E-4	0.0	0.0	2.8630912394523915	0.0
22	1.5255987848097145E-4	0.0	0.0	3.0537402342607787	0.0
23	1.5255987848097145E-4	0.0	0.0	3.259238390574647	0.0
24	1.5255987848097145E-4	0.0	0.0	3.410221816977982	0.0
25	2.0341317130796196E-4	0.0	0.0	3.550627758473303	0.0
26	2.0341317130796196E-4	0.0	0.0	3.686609463492675	0.0
27	2.0341317130796196E-4	0.0	0.0	3.8400338479517058	0.0
28	2.0341317130796196E-4	0.0	0.0	3.997323082665587	0.0
29	2.0341317130796196E-4	0.0	0.0	4.174241688410687	0.0
30	2.0341317130796196E-4	0.0	0.0	4.361432659306839	0.0
31	2.0341317130796196E-4	0.0	0.0	4.542877208113541	0.0
32	2.0341317130796196E-4	0.0	0.0	4.707845290044299	0.0
33	2.0341317130796196E-4	0.0	0.0	4.877390168329485	0.0
34	2.0341317130796196E-4	0.0	0.0	5.06198762129146	0.0
35	2.0341317130796196E-4	0.0	0.0	5.260722289659339	0.0
36	2.0341317130796196E-4	0.0	0.0	5.450405071904013	0.0
37	2.0341317130796196E-4	0.0	0.0	5.646546222337716	0.0
38	2.0341317130796196E-4	0.0	0.0	5.856621175006014	0.0
39	2.0341317130796196E-4	0.0	0.0	6.165961755272597	0.0
40	2.0341317130796196E-4	0.0	0.0	6.3984121567847705	0.0
41	2.0341317130796196E-4	0.0	0.0	6.635744474408335	0.0
42	2.0341317130796196E-4	0.0	0.0	6.874805803988017	0.0
43	2.0341317130796196E-4	0.0	0.0	7.084423077020872	0.0
44	2.0341317130796196E-4	0.0	0.0	7.294498029689169	0.0
45	2.0341317130796196E-4	0.0	0.0	7.499029973439325	0.0
46	2.0341317130796196E-4	0.0	0.0	7.74582100352871	0.0
47	2.0341317130796196E-4	0.0	0.0	7.999019548514296	0.0
48	2.5426646413495243E-4	0.0	0.0	8.223485983052631	0.0
49	2.5426646413495243E-4	0.0	0.0	8.473633330468598	0.0
50	2.5426646413495243E-4	0.0	0.0	8.71101650138499	0.0
51	2.5426646413495243E-4	0.0	0.0	8.967469657111502	0.0
52	2.5426646413495243E-4	0.0	0.0	9.242992797648137	0.0
53	2.5426646413495243E-4	0.0	0.0	9.50148008508773	0.0
54	2.5426646413495243E-4	0.0	0.0	9.754831189951796	0.0
55	3.051197569619429E-4	0.0	0.0	9.994909585388019	0.0
56	3.051197569619429E-4	0.0	0.0	10.249430315987105	0.0
57	3.051197569619429E-4	0.0	0.0	10.516104983571843	0.0
58	3.559730497889334E-4	0.0	0.0	10.774795684182743	0.0
59	3.559730497889334E-4	0.0	0.0	11.050776504354822	0.0
60	3.559730497889334E-4	0.0	0.0	11.33214777356656	0.0
61	3.559730497889334E-4	0.0	0.0	11.623079461829773	0.0
62	3.559730497889334E-4	0.0	0.0	11.966593454876094	0.0
63	4.068263426159239E-4	0.0	0.0	12.264441190963776	0.0
64	4.576796354429144E-4	0.0	0.0	12.565797804256523	0.0
65	4.576796354429144E-4	0.0	0.0	12.840914118450542	0.0
66	4.576796354429144E-4	0.0	0.0	13.123404160104473	0.0
67	4.576796354429144E-4	0.0	0.0	13.425981252425066	0.0
68	5.085329282699049E-4	0.0	0.0	13.747831742727088	0.0
69	5.085329282699049E-4	0.0	0.0	14.14067342981559	0.0
70	5.085329282699049E-4	0.0	0.0	14.443759055064454	0.0
71	5.085329282699049E-4	0.0	0.0	14.733215997835684	0.0
72	5.085329282699049E-4	0.0	0.0	15.037725515283704	0.0
73	5.085329282699049E-4	0.0	0.0	15.325555152684469	0.0
74	5.085329282699049E-4	0.0	0.0	15.649185508235437	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	5440	0.0	71.9853	1
CGTAAGG	465	0.0	67.09677	2
GACGGGT	305	0.0	65.57377	4
TACGGGA	1135	0.0	65.19824	4
CCGATCC	1420	0.0	64.788734	80
GCGGGAT	1585	0.0	64.35332	5
TAAGGGA	2945	0.0	64.244484	4
CGGGATT	1225	0.0	63.34694	6
GCGTAAG	215	0.0	63.25581	1
CTACGGG	575	0.0	62.608696	3
TAGGGTA	1770	0.0	62.598866	5
CGGGTAT	260	0.0	61.53846	6
TAGGGAC	2470	0.0	61.214573	5
TAGGGCG	335	0.0	60.895523	5
GGACGTA	125	0.0	60.8	8
TGCGGGA	1900	0.0	60.42105	4
AGGGATT	4590	0.0	60.39216	6
CGGGATG	1120	0.0	60.35714	6
CGTAGGG	1205	0.0	60.082985	3
TAGGGAT	4205	0.0	60.02378	5
>>END_MODULE