FastQCFastQC Report
Thu 26 May 2016
SRR1547830_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547830_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1312856
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64560.491752332319767No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGCG750.074.6666641
CTAGCGG1250.073.62
CGTTTTT42050.070.582641
CGAGGGA9200.070.04
TATAGCG1550.069.6774141
CGTTAGG2900.068.9655152
TACGCCA351.1816155E-768.571431
ATGCGCG650.067.692311
GATTCGC303.7958853E-666.666679
TAGCGGG11150.066.3677143
ACGGGAT7150.065.454555
GGGCGAT9100.065.054957
GCGAGGG11750.065.021283
TTAGCGG4450.064.719092
CGGGATT8700.064.367826
CGAACTA251.235592E-464.080
TCCGCGG750.064.02
TCTACCG251.235592E-464.01
GCTAGCG503.0013325E-1064.01
TACGGGA8450.062.958584
Produced by FastQC (version 0.11.5)