##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547830_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1312856
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.009553980025224	33.0	31.0	34.0	31.0	34.0
2	32.43064128891516	34.0	31.0	34.0	31.0	34.0
3	32.542734313588085	34.0	31.0	34.0	31.0	34.0
4	36.0738062666431	37.0	35.0	37.0	35.0	37.0
5	36.025506224597365	37.0	35.0	37.0	35.0	37.0
6	36.08745361258204	37.0	35.0	37.0	35.0	37.0
7	36.341208022814385	37.0	37.0	37.0	35.0	37.0
8	36.35760205231952	37.0	37.0	37.0	35.0	37.0
9	38.16204899851926	39.0	39.0	39.0	37.0	39.0
10-11	37.67340172875015	39.0	37.5	39.0	35.0	39.0
12-13	36.91701298543023	39.0	35.0	39.0	33.5	39.0
14-15	38.04811685363818	40.0	36.0	41.0	33.0	41.0
16-17	38.2373104894977	40.0	36.0	41.0	34.0	41.0
18-19	38.121160279573694	40.0	36.0	41.0	34.0	41.0
20-21	37.94152328968295	40.0	35.0	41.0	34.0	41.0
22-23	37.75086566995923	40.0	35.0	41.0	34.0	41.0
24-25	37.73851244919473	40.0	35.0	41.0	34.0	41.0
26-27	37.73965956662421	40.0	35.0	41.0	34.0	41.0
28-29	37.682369201191904	40.0	35.0	41.0	34.0	41.0
30-31	37.54574797235949	40.0	35.0	41.0	33.5	41.0
32-33	37.25373841457098	40.0	35.0	41.0	33.0	41.0
34-35	36.987283068363936	40.0	35.0	41.0	32.0	41.0
36-37	36.8330708013674	39.5	35.0	41.0	31.5	41.0
38-39	36.750385419269136	39.0	35.0	41.0	31.0	41.0
40-41	36.652948990597594	39.0	35.0	41.0	31.0	41.0
42-43	36.6195850877781	39.0	35.0	41.0	31.0	41.0
44-45	36.49082648820587	39.0	35.0	41.0	31.0	41.0
46-47	36.3712273090118	39.0	35.0	41.0	31.0	41.0
48-49	36.2834983425448	38.0	35.0	41.0	31.0	41.0
50-51	36.05644411877616	38.0	35.0	40.0	30.5	41.0
52-53	35.88760991304454	38.0	35.0	40.0	30.0	41.0
54-55	35.71573615080405	37.5	35.0	40.0	29.5	41.0
56-57	35.42355749602393	37.0	35.0	40.0	29.0	41.0
58-59	35.170396448658494	36.5	35.0	40.0	28.5	41.0
60-61	34.81483308146514	36.0	34.0	39.5	28.0	41.0
62-63	34.37744924043459	35.5	34.0	39.0	26.5	40.5
64-65	34.020470257210235	35.0	34.0	39.0	26.0	40.0
66-67	33.70737994113597	35.0	34.0	38.0	26.0	40.0
68-69	33.25066915183386	35.0	33.5	37.0	24.5	40.0
70-71	32.94845321954578	35.0	33.0	37.0	24.5	39.0
72-73	32.67555314520405	35.0	33.0	36.0	24.0	39.0
74-75	32.36952034343447	35.0	33.0	36.0	23.5	38.0
76-77	30.4004536674243	33.0	30.0	34.5	19.5	36.0
78-79	31.39810497114687	34.0	31.5	35.0	20.0	37.0
80-81	31.59194420408636	35.0	32.0	35.0	20.0	36.5
82-83	31.403622712620425	34.5	32.0	35.0	19.5	36.0
84-85	31.189775573254035	34.0	32.0	35.0	19.0	36.0
86	30.76984604556783	34.0	31.0	35.0	18.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	1.0
10	7.0
11	13.0
12	9.0
13	39.0
14	118.0
15	388.0
16	957.0
17	2167.0
18	4564.0
19	7806.0
20	9907.0
21	10090.0
22	9000.0
23	8285.0
24	8597.0
25	9596.0
26	11282.0
27	14178.0
28	17450.0
29	21792.0
30	27504.0
31	33788.0
32	42196.0
33	55642.0
34	142871.0
35	128077.0
36	108642.0
37	207264.0
38	299012.0
39	131349.0
40	261.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.37861273437452	24.648095449919868	30.62095157427776	13.352340241427848
2	30.230505097284087	24.870587482557113	31.657394261061384	13.241513159097417
3	28.852059936504844	25.039760643970094	32.955099416843886	13.153080002681177
4	26.9343324782002	27.06283095785067	32.49130140700884	13.511535156940289
5	23.950456104858418	31.715664170327894	30.061103426423003	14.272776298390685
6	21.863783994588896	40.7874130902399	26.525300566094074	10.823502349077128
7	89.80802159566625	2.9765640710024557	5.749450053928229	1.4659642794030723
8	91.14792482953196	2.0073031619614032	5.334324556539332	1.510447451967314
9	86.71484153631472	4.96474860913916	6.274031576958936	2.0463782775871837
10-11	39.89607390300231	28.64902929186445	19.848635341575925	11.606261463557313
12-13	28.55526424832579	26.558548690793206	28.04538349978977	16.84080356109124
14-15	16.106259940160992	31.986371696515082	35.18340930002986	16.723959063294068
16-17	21.694801257716	26.363401622112402	31.96691030851822	19.974886811653374
18-19	24.455309645536143	27.870345262542124	29.853426423004503	17.820918668917233
20-21	26.624130902399045	27.304213104864512	28.88926889163777	17.182387101098673
22-23	21.874219259385644	23.91016227217608	31.917019079015517	22.298599389422755
24-25	19.889119598798345	27.406090233810865	33.8478096607701	18.85698050662068
26-27	18.35700183416917	33.15062733460486	29.463398879999026	19.028971951226943
28-29	17.13436203208882	27.69903934628017	35.97892685869585	19.187671762935157
30-31	22.87265320796797	27.245143412529632	30.77237716855466	19.109826210947737
32-33	25.71793860103469	27.35924579694955	28.18492660276527	18.737888999250487
34-35	19.706692889395335	27.736971914665432	31.003057456415632	21.553277739523605
36-37	22.587892350722395	28.60058528886641	30.009193696795382	18.80232866361581
38-39	20.428173386875635	30.514199577105185	28.712859597701502	20.34476743831768
40-41	20.706459809758268	26.689103755476612	29.291255095760693	23.31318133900443
42-43	21.294757383901967	26.568184172521587	28.36266886848215	23.774389575094297
44-45	20.11846691487871	30.037186104188123	28.82254413279141	21.02180284814176
46-47	21.30568775250294	28.72005764531678	28.734339485823273	21.23991511635701
48-49	21.329871669093944	26.28982157982292	31.701763178901572	20.678543572181564
50-51	19.111006843096273	30.09214262645713	29.542691658491105	21.254158871955493
52-53	22.344263194135532	26.084201161437353	30.084716069393746	21.486819575033362
54-55	21.61965973419781	26.872939606476265	30.85902033429409	20.64838032503184
56-57	22.73006331235109	25.26034843120647	29.652985552109296	22.35660270433315
58-59	23.688850871687375	26.546970878755932	28.80925249989336	20.95492574966333
60-61	23.777284028103615	29.02188054135412	27.24110641227979	19.959729018262475
62-63	23.33469931203422	25.659325927596022	30.405162485451566	20.600812274918194
64-65	20.35676418434314	29.294987416746395	30.174482197590596	20.17376620131987
66-67	19.486981055043355	28.918061082098873	26.94731943183411	24.647638431023662
68-69	18.55732845034033	31.104058632477592	27.195214098118907	23.143398819063172
70-71	20.103537630935914	29.096412706344033	29.599666680885033	21.200382981835023
72-73	22.628224268312746	29.1022777821787	27.854616195530962	20.41488175397759
74-75	21.768228960373413	30.627959197352943	25.82792781538874	21.7758840268849
76-77	24.41387326561329	27.577586574612905	27.439681122682153	20.568859037091652
78-79	19.19890681080027	28.83842553943464	30.034101226638715	21.928566423126377
80-81	17.75503177804725	28.1157644098058	30.29067925195147	23.83852456019548
82-83	19.08796547374579	27.44859299115821	31.36912959227821	22.094311942817797
84-85	17.91875879761375	26.72406570103652	29.737153198827592	25.620022302522134
86	19.321616384432108	26.482873978562765	31.08551128227315	23.10999835473197
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	524.0
1	637.0
2	2280.0
3	2454.0
4	1178.5
5	1459.5
6	1892.5
7	2125.0
8	2279.5
9	2444.0
10	2487.0
11	2456.5
12	2377.0
13	2335.5
14	2310.0
15	2412.5
16	2544.5
17	2832.5
18	2893.5
19	2703.5
20	2814.0
21	2917.0
22	3262.5
23	3934.5
24	4477.5
25	5819.5
26	8216.5
27	11537.0
28	13677.0
29	13768.0
30	15701.5
31	20318.5
32	23472.0
33	24240.0
34	27693.0
35	32468.5
36	34089.0
37	35693.5
38	39020.0
39	43454.5
40	50232.5
41	57072.0
42	63980.5
43	68115.0
44	69996.0
45	72456.0
46	74382.5
47	77639.0
48	78746.5
49	72167.0
50	66389.0
51	59058.5
52	49079.5
53	43550.0
54	37364.0
55	33786.5
56	31517.5
57	29521.0
58	25785.0
59	19206.5
60	13444.5
61	9447.5
62	7099.5
63	4839.5
64	3850.0
65	3076.5
66	1840.5
67	1186.5
68	782.0
69	462.0
70	352.0
71	349.0
72	261.0
73	121.5
74	69.5
75	44.5
76	20.0
77	16.0
78	13.5
79	15.0
80	11.0
81	5.5
82	3.5
83	3.5
84	3.0
85	1.5
86	1.0
87	1.0
88	0.5
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1312856.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.737075136955685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.01219108428234	14.804573610816984
2	8.353639494105071	3.1304554173617447
3	2.690546338511063	1.5123890671242841
4	1.3482310308829	1.0104762451051246
5	0.7711018165419196	0.7224096337394483
6	0.5345664531644477	0.6009727079182896
7	0.3849411377713199	0.5048869715208503
8	0.3220916341841172	0.4828044120554118
9	0.2575575701593432	0.43432879897506144
>10	3.4295593058233953	15.806838138341911
>50	1.474207286996131	20.0944603528153
>100	1.415222610484661	39.62611279057709
>500	0.004915389674621109	0.5925869151058117
>1k	8.192316124368515E-4	0.181206800487608
>5k	4.0961580621842573E-4	0.4954981380550603
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6456	0.491752332319767	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.616981603466032E-5	0.0	0.0	0.06055500374755495	0.0
2	7.616981603466032E-5	0.0	0.0	0.20230703138805778	0.0
3	7.616981603466032E-5	0.0	0.0	0.2875410555308427	0.0
4	7.616981603466032E-5	0.0	0.0	0.4602941982974523	0.0
5	7.616981603466032E-5	0.0	0.0	0.7969647851706508	0.0
6	7.616981603466032E-5	0.0	0.0	1.0497724045896883	0.0
7	7.616981603466032E-5	0.0	0.0	1.2275527552145855	0.0
8	7.616981603466032E-5	0.0	0.0	1.593396381629059	0.0
9	7.616981603466032E-5	0.0	0.0	1.6958447841956772	0.0
10	7.616981603466032E-5	0.0	0.0	1.9318950440870895	0.0
11	7.616981603466032E-5	0.0	0.0	2.308707124010554	0.0
12	7.616981603466032E-5	0.0	0.0	2.625345049266637	0.0
13	7.616981603466032E-5	0.0	0.0	2.728935999073775	0.0
14	7.616981603466032E-5	0.0	0.0	2.766411548562828	0.0
15	7.616981603466032E-5	0.0	0.0	2.825747835253828	0.0
16	7.616981603466032E-5	0.0	0.0	2.97107984424796	0.0
17	7.616981603466032E-5	0.0	0.0	3.1634086297354775	0.0
18	7.616981603466032E-5	0.0	0.0	3.413321796145198	0.0
19	7.616981603466032E-5	0.0	0.0	3.572364372025569	0.0
20	7.616981603466032E-5	0.0	0.0	3.7422992315988957	0.0
21	7.616981603466032E-5	0.0	0.0	3.9494049613971374	0.0
22	7.616981603466032E-5	0.0	0.0	4.189111372458213	0.0
23	7.616981603466032E-5	0.0	0.0	4.430188840207913	0.0
24	7.616981603466032E-5	0.0	0.0	4.6072074926724635	0.0
25	1.5233963206932064E-4	0.0	0.0	4.752082482770388	0.0
26	1.5233963206932064E-4	0.0	0.0	4.902594039254876	0.0
27	1.5233963206932064E-4	0.0	0.0	5.06331235108801	0.0
28	1.5233963206932064E-4	0.0	0.0	5.23134296526047	0.0
29	1.5233963206932064E-4	0.0	0.0	5.427937260445929	0.0
30	1.5233963206932064E-4	0.0	0.0	5.66429219960148	0.0
31	1.5233963206932064E-4	0.0	0.0	5.8613435136831455	0.0
32	1.5233963206932064E-4	0.0	0.0	6.030516675096126	0.0
33	1.5233963206932064E-4	0.0	0.0	6.212029346706722	0.0
34	1.5233963206932064E-4	0.0	0.0	6.402834735873546	0.0
35	1.5233963206932064E-4	0.0	0.0	6.620985088996813	0.0
36	2.2850944810398093E-4	0.0	0.0	6.824967856337634	0.0
37	2.2850944810398093E-4	0.0	0.0	7.034663359881053	0.0
38	2.2850944810398093E-4	0.0	0.0	7.269342563083842	0.0
39	2.2850944810398093E-4	0.0	0.0	7.617667131810343	0.0
40	2.2850944810398093E-4	0.0	0.0	7.841606390952244	0.0
41	2.2850944810398093E-4	0.0	0.0	8.073467310961751	0.0
42	2.2850944810398093E-4	0.0	0.0	8.315154137239729	0.0
43	2.2850944810398093E-4	0.0	0.0	8.529419829745228	0.0
44	2.2850944810398093E-4	0.0	0.0	8.744294880779004	0.0
45	2.2850944810398093E-4	0.0	0.0	8.958027384572262	0.0
46	2.2850944810398093E-4	0.0	0.0	9.196286569128679	0.0
47	2.2850944810398093E-4	0.0	0.0	9.449627377259958	0.0
48	2.2850944810398093E-4	0.0	0.0	9.683925731382574	0.0
49	2.2850944810398093E-4	0.0	0.0	9.924012991523822	0.0
50	2.2850944810398093E-4	0.0	0.0	10.174459346645786	0.0
51	2.2850944810398093E-4	0.0	0.0	10.42574356974413	0.0
52	2.2850944810398093E-4	0.0	0.0	10.721206286142579	0.0
53	2.2850944810398093E-4	0.0	0.0	10.975004113170066	0.0
54	2.2850944810398093E-4	0.0	0.0	11.232381921551182	0.0
55	2.2850944810398093E-4	0.0	0.0	11.495472466134899	0.0
56	2.2850944810398093E-4	0.0	0.0	11.762980860048627	0.0
57	2.2850944810398093E-4	0.0	0.0	12.027290121688898	0.0
58	3.0467926413864127E-4	0.0	0.0	12.28131645816449	0.0
59	3.0467926413864127E-4	0.0	0.0	12.542578927163374	0.0
60	3.0467926413864127E-4	0.0	0.0	12.827530208949039	0.0
61	3.0467926413864127E-4	0.0	0.0	13.119641453441961	0.0
62	3.808490801733016E-4	0.0	0.0	13.439097661891328	0.0
63	3.808490801733016E-4	0.0	0.0	13.771655078698654	0.0
64	3.808490801733016E-4	0.0	0.0	14.077096040997642	0.0
65	3.808490801733016E-4	0.0	0.0	14.360066907566404	0.0
66	4.5701889620796185E-4	0.0	0.0	14.633821226394973	0.0
67	4.5701889620796185E-4	0.0	0.0	14.923495036774787	0.0
68	4.5701889620796185E-4	0.0	0.0	15.253615019469004	0.0
69	4.5701889620796185E-4	0.0	0.0	15.698979933823663	0.0
70	4.5701889620796185E-4	0.0	0.0	15.99246223500521	0.0
71	4.5701889620796185E-4	0.0	0.0	16.27276715801276	0.0
72	4.5701889620796185E-4	0.0	0.0	16.557794609614458	0.0
73	4.5701889620796185E-4	0.0	0.0	16.857446665894813	0.0
74	4.5701889620796185E-4	0.0	0.0	17.18086370477798	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTGCG	75	0.0	74.666664	1
CTAGCGG	125	0.0	73.6	2
CGTTTTT	4205	0.0	70.58264	1
CGAGGGA	920	0.0	70.0	4
TATAGCG	155	0.0	69.677414	1
CGTTAGG	290	0.0	68.965515	2
TACGCCA	35	1.1816155E-7	68.57143	1
ATGCGCG	65	0.0	67.69231	1
GATTCGC	30	3.7958853E-6	66.66667	9
TAGCGGG	1115	0.0	66.367714	3
ACGGGAT	715	0.0	65.45455	5
GGGCGAT	910	0.0	65.05495	7
GCGAGGG	1175	0.0	65.02128	3
TTAGCGG	445	0.0	64.71909	2
CGGGATT	870	0.0	64.36782	6
CGAACTA	25	1.235592E-4	64.0	80
TCCGCGG	75	0.0	64.0	2
TCTACCG	25	1.235592E-4	64.0	1
GCTAGCG	50	3.0013325E-10	64.0	1
TACGGGA	845	0.0	62.95858	4
>>END_MODULE