FastQCFastQC Report
Thu 26 May 2016
SRR1547829_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547829_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2761380
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT529931.9190766935372892No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT275350.076.106771
TACGGGA14100.064.6808554
TAGGGAT48800.064.426235
TAGCGGG16100.064.3478243
ACGGGAC11250.064.05
ACGGGAT15150.063.3663335
CGAGGGA20850.062.5419654
GTAGGGA46800.062.222224
TAGGGAC34700.061.9020165
ATAGGGA38450.061.2743874
TAAGGGA39400.061.2182734
CCGATCC23000.061.21739280
AGGGATC30850.060.9400376
CGGGATA8800.060.06
GGGCGAT16150.059.6903957
AGGGATT51150.059.589446
GCGGGAT18900.059.2592625
TTAGCGG7100.059.154932
AGTAGGG45150.059.003323
ATAGCGG4500.058.6666682
Produced by FastQC (version 0.11.5)