##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547829_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2761380
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0088339888027	33.0	31.0	34.0	31.0	34.0
2	32.43238525664704	34.0	31.0	34.0	31.0	34.0
3	32.46755861199835	34.0	31.0	34.0	31.0	34.0
4	36.078786693609715	37.0	35.0	37.0	35.0	37.0
5	36.05756397163737	37.0	35.0	37.0	35.0	37.0
6	36.12469526106512	37.0	36.0	37.0	35.0	37.0
7	36.29199603097002	37.0	36.0	37.0	35.0	37.0
8	36.27240944745019	37.0	37.0	37.0	35.0	37.0
9	38.01721784035519	39.0	39.0	39.0	35.0	39.0
10-11	37.736446450687694	39.0	37.5	39.0	35.0	39.0
12-13	37.32772327604314	39.0	37.0	39.0	34.5	39.0
14-15	38.606998674575756	40.0	38.0	41.0	34.0	41.0
16-17	38.68054958028232	40.0	38.0	41.0	34.5	41.0
18-19	38.35613986485018	40.0	37.5	41.0	34.0	41.0
20-21	38.00141813151396	40.0	35.0	41.0	34.0	41.0
22-23	37.80896001274725	40.0	35.0	41.0	33.5	41.0
24-25	37.77251138923292	40.0	35.0	41.0	34.0	41.0
26-27	37.724100992981775	40.0	35.0	41.0	33.5	41.0
28-29	37.568920793226575	40.0	35.0	41.0	33.0	41.0
30-31	37.27858443966423	40.0	35.0	41.0	33.0	41.0
32-33	36.65407875772259	40.0	35.0	41.0	30.5	41.0
34-35	36.023164323635285	40.0	35.0	41.0	27.0	41.0
36-37	35.755991750501565	40.0	35.0	41.0	25.0	41.0
38-39	35.604381867037496	39.0	35.0	41.0	24.0	41.0
40-41	35.44925055588148	39.0	35.0	41.0	23.0	41.0
42-43	35.35621482736892	39.0	35.0	41.0	23.0	41.0
44-45	35.2365999971029	39.0	35.0	41.0	23.0	41.0
46-47	35.11750157529931	39.0	35.0	41.0	23.0	41.0
48-49	35.01396312713209	38.0	35.0	40.5	23.0	41.0
50-51	34.78705502321303	38.0	34.5	40.0	22.5	41.0
52-53	34.58180149055907	38.0	34.0	40.0	22.0	41.0
54-55	34.379946077685794	37.0	34.0	40.0	21.0	41.0
56-57	34.09665638195395	37.0	34.0	40.0	20.0	41.0
58-59	33.83719571373733	36.5	34.0	40.0	20.0	41.0
60-61	33.491587177425785	36.0	33.0	39.5	19.0	41.0
62-63	33.12631564652456	35.0	33.0	39.0	18.0	40.5
64-65	32.767804322476444	35.0	33.0	39.0	17.5	40.0
66-67	32.43639339750415	35.0	32.5	38.0	16.0	40.0
68-69	31.942495237888302	35.0	32.0	37.0	13.5	40.0
70-71	31.646044550188677	35.0	32.0	37.0	11.5	39.0
72-73	31.397862300733692	35.0	31.5	36.0	11.0	39.0
74-75	31.109821719574995	35.0	31.0	36.0	10.0	38.5
76-77	29.23683846482556	33.0	28.0	34.5	10.0	36.0
78-79	30.330944129384584	34.0	30.5	35.0	10.0	37.0
80-81	30.420950213299147	34.0	31.0	35.0	10.0	36.5
82-83	30.243471742389673	34.0	31.0	35.0	10.0	36.0
84-85	30.0260900346928	34.0	30.0	35.0	10.0	36.0
86	29.601394592558794	34.0	29.0	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	14.0
10	21.0
11	30.0
12	58.0
13	178.0
14	554.0
15	1662.0
16	4302.0
17	10278.0
18	23780.0
19	40874.0
20	51600.0
21	51589.0
22	43068.0
23	33004.0
24	26814.0
25	24896.0
26	26117.0
27	29343.0
28	34678.0
29	41962.0
30	51653.0
31	64091.0
32	78117.0
33	98985.0
34	208886.0
35	215826.0
36	245271.0
37	440356.0
38	636099.0
39	276711.0
40	555.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.741346717945376	21.52438273616815	26.427836806234563	19.30643373965191
2	37.15374196959491	23.861257776908648	26.759084226002937	12.2259160274935
3	27.84546132730736	23.78897507767855	35.82335643772317	12.54220715729092
4	25.363622536557806	25.031035207034165	36.026588155197764	13.578754101210263
5	22.6005475523108	29.100268706226593	34.12004142856108	14.179142312901522
6	20.622695898427597	38.58118042428062	31.02908690582245	9.767036771469337
7	79.99398851298987	3.7668484598280574	13.988657844990549	2.250505182191513
8	80.44039574415692	4.16320825094699	13.290890786490813	2.1055052184052903
9	75.62305803619928	6.241372067589394	14.463963670338744	3.671606225872571
10-11	31.664294664262073	29.461066568165194	26.379962192816635	12.4946765747561
12-13	25.784299879045985	22.17116079641339	34.44873577703902	17.595803547501614
14-15	17.46407593304797	27.860634899941335	36.57528119997972	18.100007967030976
16-17	22.735280910269502	27.12958738022293	33.70582824529764	16.429303464209923
18-19	22.264556127733233	27.753713722848723	32.84502676198133	17.136703387436718
20-21	24.972767239568622	26.327886781247056	33.26153952009502	15.437806459089295
22-23	22.007474523607762	23.287939363651507	36.203963235773415	18.500622876967313
24-25	20.467411221925268	27.0314480440939	34.6772990316436	17.823841702337237
26-27	19.327854188847603	29.272139292672506	33.294439736653416	18.10556678182648
28-29	18.35531509607515	27.582494984391865	35.68717452867769	18.3750153908553
30-31	21.26114478992388	26.07529568549059	33.796887787990066	18.86667173659547
32-33	22.715562508600772	26.564724883934844	32.39822117926544	18.321491428198943
34-35	20.504874374407002	27.397297727947624	32.071772084972004	20.026055812673373
36-37	21.193117209511186	29.202282916512758	30.768365817091453	18.836234056884603
38-39	20.90824153140821	29.030883109170052	29.98211039407832	20.078764965343414
40-41	20.680330124792675	27.422122272197235	31.125325018650095	20.772222584359994
42-43	20.48416009386611	27.809627794798253	30.33740376188717	21.368808349448464
44-45	20.564464144739226	28.13821350194468	30.384155748212848	20.913166605103246
46-47	20.671385321831835	28.610930042225263	30.528159833126917	20.189524802815985
48-49	20.559158826383907	27.392626150692767	31.64709312010661	20.40112190281671
50-51	19.42371205701497	27.757027283459717	31.994636739601212	20.824623919924097
52-53	20.47010914832439	26.393488038589403	31.507670078004473	21.628732735081734
54-55	19.845186102600874	26.540389225677018	32.652224612331516	20.962200059390597
56-57	20.652246340597816	27.460074310670752	30.652246340597816	21.235433008133615
58-59	21.01974375131275	28.29806111437035	30.28789952849662	20.39429560582028
60-61	21.74237156783927	29.265312995676073	29.17191766435623	19.82039777212843
62-63	21.435930585431922	27.790887165113094	30.58516031839153	20.188021931063453
64-65	21.089672555026834	29.656530430436955	29.065756976584172	20.18804003795204
66-67	20.265428879763018	30.021076418312582	28.177088991736017	21.536405710188383
68-69	19.823240553636225	30.737185754948616	28.73139517197923	20.708178519435936
70-71	20.120320274645287	29.7188181271683	29.611969377630025	20.54889222055639
72-73	20.781022532212155	30.58525085283445	28.556247238699488	20.0774793762539
74-75	19.585859244290898	30.7470177954501	28.061548935677088	21.605574024581912
76-77	20.54492681195634	29.13260760923886	28.991717909161363	21.330747669643436
78-79	19.5952023988006	29.18535297568607	28.92278498431943	22.296659641193894
80-81	18.829842325214205	29.229298394281123	29.212839232557634	22.72802004794704
82-83	20.139730859208075	29.715631314777397	28.708055392593558	21.43658243342097
84-85	18.811590581520836	29.40996892857919	28.65677668412171	23.12166380577827
86	19.487068060172813	29.225314878792485	28.94433218173522	22.34328487929948
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1393.0
1	1539.0
2	14854.5
3	18135.5
4	8583.5
5	9813.5
6	11235.5
7	11764.0
8	12179.0
9	12931.0
10	13308.5
11	13414.0
12	13635.0
13	13913.0
14	14035.0
15	14101.5
16	14135.5
17	14080.0
18	14281.0
19	14266.0
20	14618.5
21	15210.0
22	15216.5
23	16088.5
24	17428.5
25	19874.0
26	23246.0
27	26786.0
28	32814.0
29	36703.0
30	40937.5
31	46420.0
32	49313.0
33	54267.0
34	61008.0
35	68478.5
36	72517.0
37	74419.5
38	81325.5
39	87933.0
40	93764.5
41	101806.5
42	109386.5
43	113152.0
44	113406.0
45	114859.5
46	118894.5
47	120072.0
48	121120.0
49	123263.0
50	122700.0
51	112093.0
52	96886.0
53	88144.5
54	81978.5
55	77190.0
56	69768.0
57	65110.0
58	59645.5
59	48234.0
60	37912.5
61	31565.5
62	27081.5
63	21502.0
64	18434.0
65	15052.0
66	9950.0
67	6983.0
68	5259.5
69	3903.5
70	2378.0
71	1732.0
72	1474.0
73	1021.5
74	574.5
75	267.0
76	188.0
77	140.0
78	110.5
79	105.0
80	75.0
81	50.0
82	40.0
83	22.5
84	14.0
85	8.0
86	8.0
87	7.0
88	6.0
89	3.5
90	0.5
91	0.5
92	0.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2761380.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.45776885953842
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.12312504331776	16.97805728623868
2	6.3164751855851895	2.7107492907859405
3	2.3785483191089893	1.5311502015805298
4	1.3657577481053598	1.1722445630787404
5	0.9110230861265313	0.9774261403903233
6	0.6967664401748533	0.8970631933413242
7	0.5347199603116839	0.8031728119064768
8	0.4529138698980426	0.7774816906841003
9	0.3820105421712472	0.7377384524235818
>10	5.507265022047	28.965139084376318
>50	1.8566083961315618	28.04657962323323
>100	0.46792800888644037	13.0718124019324
>500	0.004800864694709441	0.7205590440170361
>1k	0.0018860539872072807	0.6611409763076915
>5k	0.0	0.0
>10k+	1.7145945338248005E-4	1.9496852397035893
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52993	1.9190766935372892	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.242755433877264E-5	0.0	0.0	0.0153546415198198	0.0
2	7.242755433877264E-5	0.0	0.0	0.05229269423259385	0.0
3	7.242755433877264E-5	0.0	0.0	0.0969080677052778	0.0
4	7.242755433877264E-5	0.0	0.0	0.17469526106511962	0.0
5	7.242755433877264E-5	0.0	0.0	0.39353511649972117	0.0
6	7.242755433877264E-5	0.0	0.0	0.6582216138307658	0.0
7	7.242755433877264E-5	0.0	0.0	0.8341843570968139	0.0
8	7.242755433877264E-5	0.0	0.0	1.066206027421072	0.0
9	7.242755433877264E-5	0.0	0.0	1.2255466469663718	0.0
10	7.242755433877264E-5	0.0	0.0	1.470206925522746	3.621377716938632E-5
11	7.242755433877264E-5	0.0	0.0	1.6627917925095423	3.621377716938632E-5
12	7.242755433877264E-5	0.0	0.0	1.83937016998747	3.621377716938632E-5
13	7.242755433877264E-5	0.0	0.0	1.908176346609304	3.621377716938632E-5
14	7.242755433877264E-5	0.0	0.0	1.9518139480984145	3.621377716938632E-5
15	7.242755433877264E-5	0.0	0.0	2.0011008988259493	3.621377716938632E-5
16	7.242755433877264E-5	0.0	0.0	2.066430552839522	3.621377716938632E-5
17	7.242755433877264E-5	0.0	0.0	2.135997218781913	3.621377716938632E-5
18	7.242755433877264E-5	0.0	0.0	2.2222584359993918	3.621377716938632E-5
19	7.242755433877264E-5	0.0	0.0	2.299719705364709	3.621377716938632E-5
20	7.242755433877264E-5	0.0	0.0	2.3746460103281692	3.621377716938632E-5
21	7.242755433877264E-5	0.0	0.0	2.4625730612954393	3.621377716938632E-5
22	7.242755433877264E-5	0.0	0.0	2.5669049533204413	3.621377716938632E-5
23	7.242755433877264E-5	0.0	0.0	2.6695710115956515	3.621377716938632E-5
24	7.242755433877264E-5	0.0	0.0	2.7525367750907157	3.621377716938632E-5
25	7.242755433877264E-5	0.0	0.0	2.8268836596194657	3.621377716938632E-5
26	7.242755433877264E-5	0.0	0.0	2.8977540215399546	3.621377716938632E-5
27	7.242755433877264E-5	0.0	0.0	2.982095908567455	3.621377716938632E-5
28	7.242755433877264E-5	0.0	0.0	3.0677777053502235	3.621377716938632E-5
29	7.242755433877264E-5	0.0	0.0	3.1685606472126255	3.621377716938632E-5
30	7.242755433877264E-5	0.0	0.0	3.2791575226879313	3.621377716938632E-5
31	1.0864133150815897E-4	0.0	0.0	3.3805923125393824	3.621377716938632E-5
32	1.0864133150815897E-4	0.0	0.0	3.482606522825544	3.621377716938632E-5
33	1.0864133150815897E-4	0.0	0.0	3.5790438114276197	3.621377716938632E-5
34	1.0864133150815897E-4	0.0	0.0	3.689785542011603	3.621377716938632E-5
35	1.0864133150815897E-4	0.0	0.0	3.8071906075947535	3.621377716938632E-5
36	1.4485510867754528E-4	0.0	0.0	3.919091179048157	3.621377716938632E-5
37	1.810688858469316E-4	0.0	0.0	4.037111878843187	3.621377716938632E-5
38	1.810688858469316E-4	0.0	0.0	4.183777676379202	3.621377716938632E-5
39	1.810688858469316E-4	0.0	0.0	4.430067574908198	3.621377716938632E-5
40	1.810688858469316E-4	0.0	0.0	4.57926833684607	3.621377716938632E-5
41	1.810688858469316E-4	0.0	0.0	4.728432885006772	3.621377716938632E-5
42	1.810688858469316E-4	0.0	0.0	4.87136866349434	3.621377716938632E-5
43	1.810688858469316E-4	0.0	0.0	5.007532465651233	3.621377716938632E-5
44	1.810688858469316E-4	0.0	0.0	5.138155559901209	3.621377716938632E-5
45	1.810688858469316E-4	0.0	0.0	5.260087347630533	3.621377716938632E-5
46	1.810688858469316E-4	0.0	0.0	5.412040356633278	3.621377716938632E-5
47	1.810688858469316E-4	0.0	0.0	5.577283821857187	3.621377716938632E-5
48	1.810688858469316E-4	0.0	0.0	5.732496070805177	3.621377716938632E-5
49	1.810688858469316E-4	0.0	0.0	5.891438338801613	3.621377716938632E-5
50	1.810688858469316E-4	0.0	0.0	6.042377362043616	3.621377716938632E-5
51	2.1728266301631794E-4	0.0	0.0	6.2080191788163885	3.621377716938632E-5
52	2.1728266301631794E-4	0.0	0.0	6.394483917461559	3.621377716938632E-5
53	2.1728266301631794E-4	0.0	0.0	6.5494788837465325	3.621377716938632E-5
54	2.1728266301631794E-4	0.0	0.0	6.708783289514663	3.621377716938632E-5
55	2.5349644018570427E-4	0.0	0.0	6.857259775909147	3.621377716938632E-5
56	2.5349644018570427E-4	0.0	0.0	7.008379868036996	3.621377716938632E-5
57	2.8971021735509057E-4	0.0	0.0	7.166199508941181	3.621377716938632E-5
58	2.8971021735509057E-4	0.0	0.0	7.321158261448986	3.621377716938632E-5
59	2.8971021735509057E-4	0.0	0.0	7.484156472488394	3.621377716938632E-5
60	2.8971021735509057E-4	0.0	0.0	7.65428879763017	3.621377716938632E-5
61	2.8971021735509057E-4	0.0	0.0	7.836118172797659	3.621377716938632E-5
62	3.2592399452447687E-4	0.0	0.0	8.057492992634117	3.621377716938632E-5
63	3.2592399452447687E-4	0.0	0.0	8.265722211358089	3.621377716938632E-5
64	3.2592399452447687E-4	0.0	0.0	8.459900484540338	3.621377716938632E-5
65	3.2592399452447687E-4	0.0	0.0	8.638434405985413	3.621377716938632E-5
66	3.2592399452447687E-4	0.0	0.0	8.831634907184089	3.621377716938632E-5
67	3.621377716938632E-4	0.0	0.0	9.014405840557982	3.621377716938632E-5
68	3.621377716938632E-4	0.0	0.0	9.220679515314806	3.621377716938632E-5
69	3.621377716938632E-4	0.0	0.0	9.495035091150077	3.621377716938632E-5
70	3.621377716938632E-4	0.0	0.0	9.700113711260311	3.621377716938632E-5
71	3.621377716938632E-4	0.0	0.0	9.903345428734909	3.621377716938632E-5
72	3.621377716938632E-4	0.0	0.0	10.088216761184626	3.621377716938632E-5
73	3.621377716938632E-4	0.0	0.0	10.278266663769564	3.621377716938632E-5
74	3.983515488632495E-4	0.0	0.0	10.49391970681326	3.621377716938632E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	27535	0.0	76.10677	1
TACGGGA	1410	0.0	64.680855	4
TAGGGAT	4880	0.0	64.42623	5
TAGCGGG	1610	0.0	64.347824	3
ACGGGAC	1125	0.0	64.0	5
ACGGGAT	1515	0.0	63.366333	5
CGAGGGA	2085	0.0	62.541965	4
GTAGGGA	4680	0.0	62.22222	4
TAGGGAC	3470	0.0	61.902016	5
ATAGGGA	3845	0.0	61.274387	4
TAAGGGA	3940	0.0	61.218273	4
CCGATCC	2300	0.0	61.217392	80
AGGGATC	3085	0.0	60.940037	6
CGGGATA	880	0.0	60.0	6
GGGCGAT	1615	0.0	59.690395	7
AGGGATT	5115	0.0	59.58944	6
GCGGGAT	1890	0.0	59.259262	5
TTAGCGG	710	0.0	59.15493	2
AGTAGGG	4515	0.0	59.00332	3
ATAGCGG	450	0.0	58.666668	2
>>END_MODULE