FastQCFastQC Report
Thu 26 May 2016
SRR1547828_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547828_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2844177
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231720.8147172275143213No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT138400.074.248551
CGAACGG1150.066.086962
CCGATCC18750.065.2880
GGGCGAT21300.064.225367
GCTAGCG950.063.1578941
GCGAACG900.062.2222251
TAGGGAT49900.061.803615
ATACGCG1750.061.7142871
AGGGATT59600.061.6778536
TAGGGCG5000.061.65
TAGGGTA20150.061.5384645
TTTCGCG850.061.1764681
TAGGGAC36650.061.1186945
TACGGGA12000.061.04
TAACGGG7650.060.65363
TTAGGGA44200.060.5429844
GAGGGAT48300.060.0414055
GTAGGGA48200.060.04
AGCGGGA23550.058.938434
TTACGGG9050.058.784533
Produced by FastQC (version 0.11.5)