##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547828_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2844177
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0264927253121	33.0	31.0	34.0	31.0	34.0
2	32.44768029556529	34.0	31.0	34.0	31.0	34.0
3	32.55195474824527	34.0	31.0	34.0	31.0	34.0
4	36.10044135790424	37.0	35.0	37.0	35.0	37.0
5	36.058945346931644	37.0	35.0	37.0	35.0	37.0
6	36.11720121497361	37.0	36.0	37.0	35.0	37.0
7	36.33941980404173	37.0	37.0	37.0	35.0	37.0
8	36.33774902194906	37.0	37.0	37.0	35.0	37.0
9	38.13745100955391	39.0	39.0	39.0	37.0	39.0
10-11	37.69230624535675	39.0	37.5	39.0	35.0	39.0
12-13	37.18331102459516	39.0	37.0	39.0	34.0	39.0
14-15	38.43412804477359	40.0	37.0	41.0	34.0	41.0
16-17	38.52855694283443	40.0	37.0	41.0	34.0	41.0
18-19	38.3600157444491	40.0	37.0	41.0	34.0	41.0
20-21	38.147817101396996	40.0	35.5	41.0	34.0	41.0
22-23	37.95404312038245	40.0	35.0	41.0	34.0	41.0
24-25	37.931832828969505	40.0	35.0	41.0	34.0	41.0
26-27	37.9205924947709	40.0	35.0	41.0	34.0	41.0
28-29	37.82644434576329	40.0	35.0	41.0	34.0	41.0
30-31	37.65333240512106	40.0	35.5	41.0	33.0	41.0
32-33	37.27116420672834	40.0	35.0	41.0	32.5	41.0
34-35	36.910689102682426	40.0	35.0	41.0	31.5	41.0
36-37	36.70567689704262	40.0	35.0	41.0	31.0	41.0
38-39	36.593768601602505	40.0	35.0	41.0	31.0	41.0
40-41	36.50398533565246	40.0	35.0	41.0	30.0	41.0
42-43	36.448716799270926	39.5	35.0	41.0	30.0	41.0
44-45	36.32536986973736	39.0	35.0	41.0	30.0	41.0
46-47	36.18754089495837	39.0	35.0	41.0	30.0	41.0
48-49	36.073093200598976	39.0	35.0	41.0	29.5	41.0
50-51	35.84925217382744	38.5	35.0	40.5	29.0	41.0
52-53	35.65914480709182	38.0	35.0	40.0	28.0	41.0
54-55	35.47582991494552	38.0	35.0	40.0	28.0	41.0
56-57	35.20396796683188	37.5	35.0	40.0	27.5	41.0
58-59	34.94385669386962	37.0	34.5	40.0	27.0	41.0
60-61	34.59542848423287	36.5	34.0	40.0	26.0	41.0
62-63	34.22762560136026	36.0	34.0	39.0	26.0	41.0
64-65	33.863704860843754	35.0	34.0	39.0	25.0	40.0
66-67	33.528385539999796	35.0	34.0	38.5	24.5	40.0
68-69	33.04357042476611	35.0	33.0	37.5	22.5	40.0
70-71	32.72757444420653	35.0	33.0	37.0	22.0	39.0
72-73	32.45053824006031	35.0	33.0	36.5	21.0	39.0
74-75	32.133719350096705	35.0	33.0	36.0	20.0	38.5
76-77	30.18505036782169	33.0	30.0	34.5	18.5	36.0
78-79	31.219637877670763	34.0	31.5	35.0	19.0	37.0
80-81	31.318495649180765	35.0	32.0	35.0	18.5	36.5
82-83	31.090303451578436	34.5	32.0	35.0	17.5	36.0
84-85	30.850653106329176	34.0	31.5	35.0	16.0	36.0
86	30.424999920891	34.0	31.0	35.0	15.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	7.0
11	13.0
12	37.0
13	122.0
14	340.0
15	1133.0
16	3061.0
17	7608.0
18	17383.0
19	27836.0
20	30954.0
21	28000.0
22	23457.0
23	19819.0
24	19047.0
25	20276.0
26	23309.0
27	28374.0
28	34954.0
29	43743.0
30	55120.0
31	68506.0
32	83580.0
33	108738.0
34	246584.0
35	245639.0
36	245935.0
37	463380.0
38	690642.0
39	305921.0
40	654.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.821120837416238	23.767297182981228	29.957734697946016	14.45384728165652
2	31.406554514715506	25.02347779340034	30.421243122351388	13.148724569532769
3	28.580710694165656	25.140981028958464	33.5645777319766	12.71373054489928
4	26.17516420391558	26.481052339569583	33.03036344081258	14.313420015702258
5	23.871369468215235	30.490085532651452	31.696023137800495	13.942521861332821
6	21.347897827737164	40.76898167730067	27.230935346147582	10.652185148814578
7	86.94008846847436	3.496617826527674	8.010788358108515	1.5525053468894519
8	88.03762916302325	2.8867050116782464	7.534692812718759	1.5409730125797374
9	83.89375907336287	5.359652370439673	8.271848060089088	2.4747404961083648
10-11	35.76027441330128	28.825895856692462	22.08656493600785	13.327264793998406
12-13	26.533809253080943	24.640783608052523	30.456894911955196	18.368512226911335
14-15	18.075580387577848	30.63181370217114	33.29052657411968	18.00207933613133
16-17	22.22249177881686	27.68424749936449	31.979567375729427	18.11369334608922
18-19	23.548270729986214	28.829904046056203	29.767134745833328	17.85469047812425
20-21	25.828121808171574	27.708384534436497	29.75586610819228	16.707627549199646
22-23	22.128510286103854	25.083055660741227	32.0678178608434	20.72061619231152
24-25	20.120618372203978	27.98452768586484	33.301847951094466	18.593005990836716
26-27	18.92394531001411	31.834059553958845	30.248486644818517	18.99350849120853
28-29	18.195843648268024	28.06987750762347	34.671031373926446	19.06324747018206
30-31	21.59223916092423	28.075503036555038	31.11778908274696	19.21446871977377
32-33	23.24987157972236	27.977372716255	29.578503728846695	19.194251975175945
34-35	19.9032268385547	28.569653013859547	30.43826034736938	21.088859800216373
36-37	21.960975002610596	29.257725521301943	29.620185382274027	19.16111409381343
38-39	20.667226406795358	30.200423532009435	28.78224526813908	20.35010479305613
40-41	20.784729642353483	27.774502079160335	29.116788441788255	22.323979836697927
42-43	20.862010346050898	27.51899758699968	29.042127124999606	22.576864941949815
44-45	20.392507217377823	29.781743541277493	28.807208552772913	21.018540688571775
46-47	20.935652035720704	29.260801982436398	29.081523407298494	20.72202257454441
48-49	21.07905028414195	27.58820917263588	30.639179629115908	20.69356091410626
50-51	19.32884275486371	29.556423527790287	30.1382438575377	20.976489859808304
52-53	21.496148094861887	26.374677103429217	30.538377182573377	21.590797619135518
54-55	20.91481999889599	27.35840631578133	30.624219238113522	21.102554447209158
56-57	21.42672555189076	27.02185904745028	29.41745538340265	22.13396001725631
58-59	21.959902636158017	28.024767797503458	29.170283705971883	20.845045860366636
60-61	22.563662528738544	29.477630963192514	27.515393732527897	20.443312775541045
62-63	21.80186043273678	27.490430447894067	29.68489654476497	21.02281257460418
64-65	20.744102775600815	29.738057793168288	29.37735590998732	20.14048352124358
66-67	19.634871528741	29.765851422045813	27.570980990282955	23.028296058930227
68-69	19.31706430366324	30.776407375490344	27.70372589329004	22.202802427556374
70-71	19.67319544458731	29.657542410335218	29.20616403268854	21.463098112388927
72-73	21.32555393001209	29.791078403348315	28.164069957671412	20.71929770896818
74-75	20.448112054910787	30.421981473023656	27.31969564482098	21.81021082724458
76-77	22.286148154633135	28.978330110960044	27.90519014815182	20.830331586255006
78-79	19.393589076910473	29.31642439974727	29.37740864932105	21.912577874021203
80-81	18.40678340342391	28.955458819897633	29.495650235551444	23.14210754112701
82-83	19.501124578392975	28.83032596072607	29.47615426184798	22.192395199032973
84-85	18.063503080152888	28.539925609411792	28.892154039639586	24.504417270795734
86	19.057112127691067	28.007258338703956	29.893709146793608	23.04192038681137
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	920.0
1	1131.5
2	6265.0
3	7313.0
4	3736.0
5	4602.5
6	5538.0
7	5904.0
8	6182.0
9	6593.5
10	6661.5
11	6686.5
12	6864.5
13	7036.5
14	7121.0
15	7209.0
16	7545.5
17	7873.5
18	8246.5
19	9007.0
20	9569.5
21	9665.0
22	10335.5
23	12386.0
24	14003.5
25	16400.0
26	21340.0
27	25242.5
28	28471.0
29	30578.0
30	36069.5
31	44089.0
32	49634.0
33	56718.0
34	63986.0
35	73752.5
36	80318.0
37	81618.5
38	90383.5
39	99013.0
40	107080.0
41	118748.0
42	129709.0
43	135904.0
44	140400.0
45	146918.0
46	153314.0
47	160873.5
48	161420.5
49	147714.5
50	136647.0
51	123706.5
52	106978.5
53	97667.5
54	85339.0
55	76071.5
56	65697.0
57	57785.0
58	51523.5
59	37761.5
60	26373.5
61	19910.0
62	15555.0
63	10693.5
64	7611.0
65	6347.5
66	4278.5
67	2957.0
68	2000.0
69	1183.5
70	738.5
71	669.0
72	456.5
73	223.5
74	213.5
75	153.0
76	71.0
77	37.5
78	17.5
79	20.0
80	23.0
81	15.5
82	11.5
83	10.5
84	5.5
85	6.5
86	5.0
87	3.5
88	1.5
89	1.0
90	0.5
91	0.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2844177.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.332078665796143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.920564591569	15.25698563033258
2	8.279706240206606	3.201278647307146
3	2.8191075085325723	1.634976243668648
4	1.438752994865057	1.1125634430952431
5	0.8908320880303278	0.8610818001908864
6	0.6076981039715382	0.7048840530619764
7	0.46105700880577777	0.6239233254554973
8	0.35302748968572084	0.5459804161434313
9	0.3011626872919226	0.523989068573701
>10	3.3579963586591335	15.389458679812154
>50	1.2401706398772505	17.379677531474506
>100	1.3240200803577975	40.94818955523273
>500	0.00442815611043344	0.595921024236262
>1k	0.0012915455322097533	0.3945698431855828
>5k	0.0	0.0
>10k+	1.8450650460139336E-4	0.8265207382296441
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23172	0.8147172275143213	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.515955582229939E-5	0.0	0.0	0.02633450731090224	0.0
2	3.515955582229939E-5	0.0	0.0	0.09036005846330943	0.0
3	3.515955582229939E-5	0.0	0.0	0.1370871081511453	0.0
4	3.515955582229939E-5	0.0	0.0	0.24921093166845804	0.0
5	3.515955582229939E-5	0.0	0.0	0.5240883390871947	0.0
6	3.515955582229939E-5	0.0	0.0	0.7600441182106458	0.0
7	3.515955582229939E-5	0.0	0.0	0.9227273830004251	0.0
8	3.515955582229939E-5	0.0	0.0	1.2063595198189143	0.0
9	3.515955582229939E-5	0.0	0.0	1.2971766525079136	0.0
10	3.515955582229939E-5	0.0	0.0	1.5260653609110826	0.0
11	3.515955582229939E-5	0.0	0.0	1.7956336754006519	0.0
12	3.515955582229939E-5	0.0	0.0	2.022412810454483	0.0
13	3.515955582229939E-5	0.0	0.0	2.106655106204712	0.0
14	3.515955582229939E-5	0.0	0.0	2.1392128548961615	0.0
15	3.515955582229939E-5	0.0	0.0	2.190088732171029	0.0
16	7.031911164459877E-5	0.0	0.0	2.3040760121469233	0.0
17	7.031911164459877E-5	0.0	0.0	2.4563872079691245	0.0
18	7.031911164459877E-5	0.0	0.0	2.6460026925187847	0.0
19	7.031911164459877E-5	0.0	0.0	2.783511715339798	1.0547866746689815E-4
20	7.031911164459877E-5	0.0	0.0	2.921055897716633	1.0547866746689815E-4
21	7.031911164459877E-5	0.0	0.0	3.1185119632146665	1.0547866746689815E-4
22	1.0547866746689815E-4	0.0	0.0	3.3274300439107694	1.0547866746689815E-4
23	1.0547866746689815E-4	0.0	0.0	3.554842050969402	1.0547866746689815E-4
24	1.0547866746689815E-4	0.0	0.0	3.7259987687123552	1.0547866746689815E-4
25	1.0547866746689815E-4	0.0	0.0	3.879575708544159	1.0547866746689815E-4
26	1.0547866746689815E-4	0.0	0.0	4.018420794486419	1.0547866746689815E-4
27	1.0547866746689815E-4	0.0	0.0	4.167251194282213	1.0547866746689815E-4
28	1.0547866746689815E-4	0.0	0.0	4.3342942439939565	1.0547866746689815E-4
29	1.0547866746689815E-4	0.0	0.0	4.521096964077834	1.0547866746689815E-4
30	1.0547866746689815E-4	0.0	0.0	4.725655259851972	1.0547866746689815E-4
31	1.0547866746689815E-4	0.0	0.0	4.932569245866203	1.0547866746689815E-4
32	1.0547866746689815E-4	0.0	0.0	5.113465160571933	1.0547866746689815E-4
33	1.0547866746689815E-4	0.0	0.0	5.304311229575375	1.0547866746689815E-4
34	1.0547866746689815E-4	0.0	0.0	5.500009317282293	1.0547866746689815E-4
35	1.0547866746689815E-4	0.0	0.0	5.715924149587034	1.0547866746689815E-4
36	1.0547866746689815E-4	0.0	0.0	5.913520853308356	1.0547866746689815E-4
37	1.0547866746689815E-4	0.0	0.0	6.1227553700068595	1.0547866746689815E-4
38	1.0547866746689815E-4	0.0	0.0	6.355546789106304	1.0547866746689815E-4
39	1.0547866746689815E-4	0.0	0.0	6.680632042239284	1.0547866746689815E-4
40	1.0547866746689815E-4	0.0	0.0	6.92629185876969	1.0547866746689815E-4
41	1.0547866746689815E-4	0.0	0.0	7.162106999669851	1.0547866746689815E-4
42	1.0547866746689815E-4	0.0	0.0	7.395918045888143	1.0547866746689815E-4
43	1.0547866746689815E-4	0.0	0.0	7.6254044667402905	1.0547866746689815E-4
44	1.0547866746689815E-4	0.0	0.0	7.852992271578035	1.0547866746689815E-4
45	1.0547866746689815E-4	0.0	0.0	8.082373213762716	1.0547866746689815E-4
46	1.0547866746689815E-4	0.0	0.0	8.331127071205485	1.0547866746689815E-4
47	1.0547866746689815E-4	0.0	0.0	8.582588214446568	1.0547866746689815E-4
48	1.0547866746689815E-4	0.0	0.0	8.834365793690056	1.0547866746689815E-4
49	1.0547866746689815E-4	0.0	0.0	9.090538317411328	1.0547866746689815E-4
50	1.0547866746689815E-4	0.0	0.0	9.360423067903298	1.0547866746689815E-4
51	1.4063822328919755E-4	3.515955582229939E-5	0.0	9.622010163221207	1.0547866746689815E-4
52	1.4063822328919755E-4	3.515955582229939E-5	0.0	9.91963580325697	1.0547866746689815E-4
53	1.4063822328919755E-4	3.515955582229939E-5	0.0	10.190680819091076	1.0547866746689815E-4
54	1.4063822328919755E-4	3.515955582229939E-5	0.0	10.469601575429378	1.0547866746689815E-4
55	1.4063822328919755E-4	3.515955582229939E-5	0.0	10.739310528142235	1.0547866746689815E-4
56	1.4063822328919755E-4	3.515955582229939E-5	0.0	11.02712665210358	1.0547866746689815E-4
57	1.4063822328919755E-4	3.515955582229939E-5	0.0	11.296941083483905	1.0547866746689815E-4
58	1.4063822328919755E-4	3.515955582229939E-5	0.0	11.570447268225571	1.0547866746689815E-4
59	1.4063822328919755E-4	3.515955582229939E-5	0.0	11.855802223279353	1.0547866746689815E-4
60	1.4063822328919755E-4	3.515955582229939E-5	0.0	12.15666254245077	1.0547866746689815E-4
61	1.7579777911149694E-4	3.515955582229939E-5	0.0	12.464836049233222	1.0547866746689815E-4
62	2.109573349337963E-4	3.515955582229939E-5	0.0	12.782924550757565	1.0547866746689815E-4
63	2.109573349337963E-4	3.515955582229939E-5	0.0	13.110752249244685	1.0547866746689815E-4
64	2.109573349337963E-4	3.515955582229939E-5	0.0	13.426168624526532	1.0547866746689815E-4
65	2.109573349337963E-4	3.515955582229939E-5	0.0	13.734306971753165	1.0547866746689815E-4
66	2.109573349337963E-4	3.515955582229939E-5	0.0	14.03822617228112	1.0547866746689815E-4
67	2.109573349337963E-4	3.515955582229939E-5	0.0	14.33026144294114	1.0547866746689815E-4
68	2.109573349337963E-4	3.515955582229939E-5	0.0	14.666773551716366	1.0547866746689815E-4
69	2.109573349337963E-4	3.515955582229939E-5	0.0	15.057431376457934	1.0547866746689815E-4
70	2.812764465783951E-4	3.515955582229939E-5	0.0	15.384169128714563	1.0547866746689815E-4
71	3.1643600240069446E-4	3.515955582229939E-5	0.0	15.688510243912386	1.0547866746689815E-4
72	3.1643600240069446E-4	3.515955582229939E-5	0.0	15.996086038245862	1.0547866746689815E-4
73	3.1643600240069446E-4	3.515955582229939E-5	0.0	16.308970925508504	1.0547866746689815E-4
74	3.1643600240069446E-4	3.515955582229939E-5	0.0	16.64361957782515	1.0547866746689815E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	13840	0.0	74.24855	1
CGAACGG	115	0.0	66.08696	2
CCGATCC	1875	0.0	65.28	80
GGGCGAT	2130	0.0	64.22536	7
GCTAGCG	95	0.0	63.157894	1
GCGAACG	90	0.0	62.222225	1
TAGGGAT	4990	0.0	61.80361	5
ATACGCG	175	0.0	61.714287	1
AGGGATT	5960	0.0	61.677853	6
TAGGGCG	500	0.0	61.6	5
TAGGGTA	2015	0.0	61.538464	5
TTTCGCG	85	0.0	61.176468	1
TAGGGAC	3665	0.0	61.118694	5
TACGGGA	1200	0.0	61.0	4
TAACGGG	765	0.0	60.6536	3
TTAGGGA	4420	0.0	60.542984	4
GAGGGAT	4830	0.0	60.041405	5
GTAGGGA	4820	0.0	60.0	4
AGCGGGA	2355	0.0	58.93843	4
TTACGGG	905	0.0	58.78453	3
>>END_MODULE