FastQCFastQC Report
Thu 26 May 2016
SRR1547827_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547827_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1912100
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145310.7599497934208461No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAC150.001309924980.09
CGTTTTT71550.074.353591
CAGCGCG1200.070.01
ACGGGAT13400.069.253735
TATCGGG1500.066.6666643
CGTAAGG3400.065.8823552
TACGGGA13200.065.7575764
CGGGTAG1400.065.714296
AGCGGGA17300.065.4335254
CGGGACG1050.064.761916
TAGGGGT28750.064.139135
TCGGTCG251.2357357E-464.02
ATAGGGA35450.063.9774324
TATCGCG700.062.8571431
TCGATAG1850.062.70271
TCGAGCG1150.062.6086961
TAGGGAC26550.062.523545
CCGATCC14400.061.94444780
AGGGATC27700.061.8050546
GTACGGG7450.061.7449653
Produced by FastQC (version 0.11.5)