##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547827_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1912100
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.054122169342605	33.0	31.0	34.0	31.0	34.0
2	32.47569530882276	34.0	31.0	34.0	31.0	34.0
3	32.578364625281104	34.0	31.0	34.0	31.0	34.0
4	36.107545107473456	37.0	35.0	37.0	35.0	37.0
5	36.06969876052508	37.0	35.0	37.0	35.0	37.0
6	36.127303488311284	37.0	36.0	37.0	35.0	37.0
7	36.36615030594634	37.0	37.0	37.0	35.0	37.0
8	36.37593588201454	37.0	37.0	37.0	35.0	37.0
9	38.177970817425866	39.0	39.0	39.0	37.0	39.0
10-11	37.71670728518383	39.0	37.5	39.0	35.0	39.0
12-13	37.08899586841692	39.0	35.0	39.0	33.5	39.0
14-15	38.29805371057999	40.0	37.0	41.0	33.5	41.0
16-17	38.444144134720986	40.0	36.5	41.0	34.0	41.0
18-19	38.319754196956225	40.0	36.0	41.0	34.0	41.0
20-21	38.127057946760104	40.0	35.0	41.0	34.0	41.0
22-23	37.93341221693426	40.0	35.0	41.0	34.0	41.0
24-25	37.92962528110455	40.0	35.0	41.0	34.0	41.0
26-27	37.88941007269494	40.0	35.0	41.0	34.0	41.0
28-29	37.83322551121803	40.0	35.0	41.0	34.0	41.0
30-31	37.69253935463627	40.0	35.5	41.0	33.5	41.0
32-33	37.375764081376495	40.0	35.0	41.0	33.0	41.0
34-35	37.073689922075204	40.0	35.0	41.0	32.5	41.0
36-37	36.87899691438732	40.0	35.0	41.0	31.5	41.0
38-39	36.785034778515765	40.0	35.0	41.0	31.0	41.0
40-41	36.69070550703415	40.0	35.0	41.0	31.0	41.0
42-43	36.651158150724335	39.5	35.0	41.0	31.0	41.0
44-45	36.52057476073427	39.0	35.0	41.0	31.0	41.0
46-47	36.379495580775064	39.0	35.0	41.0	31.0	41.0
48-49	36.29044636786779	39.0	35.0	41.0	31.0	41.0
50-51	36.0689184666074	38.5	35.0	40.5	30.0	41.0
52-53	35.87747947283092	38.0	35.0	40.0	29.5	41.0
54-55	35.70156764813556	38.0	35.0	40.0	29.0	41.0
56-57	35.407459599393334	37.0	35.0	40.0	28.5	41.0
58-59	35.16251085194289	37.0	35.0	40.0	28.0	41.0
60-61	34.80428167982846	36.0	34.0	39.5	27.5	41.0
62-63	34.40829663720517	36.0	34.0	39.0	26.5	41.0
64-65	34.052767637675856	35.0	34.0	39.0	26.0	40.0
66-67	33.74995868416924	35.0	34.0	38.5	25.5	40.0
68-69	33.28850661576277	35.0	33.5	37.5	24.0	40.0
70-71	32.97752471105068	35.0	33.0	37.0	24.0	39.5
72-73	32.682780973798444	35.0	33.0	36.5	23.0	39.0
74-75	32.37201506197374	35.0	33.0	36.0	22.5	38.5
76-77	30.405027718215575	33.0	30.0	34.5	19.5	36.0
78-79	31.421702316824433	34.0	31.5	35.0	20.0	37.0
80-81	31.56263950630197	35.0	32.0	35.0	20.0	37.0
82-83	31.368221327336435	35.0	32.0	35.0	19.0	36.0
84-85	31.147766591705455	34.0	32.0	35.0	18.5	36.0
86	30.741812143716334	34.0	31.0	35.0	18.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	7.0
11	14.0
12	23.0
13	70.0
14	221.0
15	615.0
16	1800.0
17	4391.0
18	9550.0
19	14525.0
20	16474.0
21	15925.0
22	13723.0
23	12136.0
24	12118.0
25	13486.0
26	16022.0
27	19340.0
28	23800.0
29	29680.0
30	37562.0
31	47095.0
32	57429.0
33	73365.0
34	182733.0
35	176130.0
36	156250.0
37	295369.0
38	461334.0
39	220366.0
40	542.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.62303226818681	24.664400397468754	29.8682600282412	13.844307306103238
2	29.39882851315308	26.345693216881962	31.619319073270226	12.636159196694733
3	28.50091522409916	25.5104335547304	33.38408033052664	12.604570890643796
4	26.191307985983997	27.976413367501703	32.21322106584384	13.619057580670468
5	23.73170859264683	32.51650018304482	30.42607604204801	13.325715182260343
6	21.155274305737148	41.49563307358402	26.845457873542177	10.503634747136656
7	89.21322106584384	2.885100151665708	6.597405993410386	1.3042727890800692
8	90.2854453218974	2.5292610219130798	5.884420270906333	1.3008733852831964
9	86.00052298519952	5.179227027875111	6.800167355263846	2.020082631661524
10-11	37.715417603681814	30.022357617279432	20.619449819570107	11.642774959468646
12-13	27.40868678416401	26.053841326290467	29.689843627425343	16.847628262120182
14-15	16.210579990586265	32.361199728047694	34.490507818628735	16.937712462737302
16-17	21.15255478269965	27.482296950996286	32.353930233774385	19.01121803252968
18-19	22.791721144291614	29.128262120181997	30.29177344281157	17.788243292714817
20-21	25.937973955337064	27.81567386642958	30.016081794885203	16.23027038334815
22-23	20.906751738925788	25.315961508289313	32.8459808587417	20.9313058940432
24-25	19.504759165315622	28.637335913393652	33.660765650332095	18.197139270958633
26-27	18.323309450342556	34.20124470477485	29.907013231525546	17.56843261335704
28-29	16.83740390146959	29.115971967993303	36.05407666963025	17.992547460906856
30-31	21.75830239004236	29.0436954134198	30.894696930076883	18.30330526646096
32-33	24.02225302023953	28.923173474190676	29.1616285759113	17.89294492965849
34-35	19.56618377699911	28.85968307096909	30.78113069400136	20.793002458030436
36-37	21.848648083259246	29.99550232728414	30.2120704984049	17.943779091051724
38-39	19.524841796977146	31.472987814444853	28.4299984310444	20.5721719575336
40-41	20.37908582187124	28.27203075152973	29.574917629831077	21.773965796767953
42-43	20.80696616285759	28.058809685685894	28.503582448616704	22.63064170283981
44-45	19.74381570001569	31.16738141310601	28.67930547565504	20.409497411223263
46-47	20.786072904136812	30.38251137492809	28.457742795878875	20.373672925056223
48-49	20.9806495476178	28.06704670257832	30.81583076198944	20.136472987814443
50-51	18.995057789864546	31.445871031849798	29.079519899586842	20.479551278698814
52-53	21.709743214267036	27.40233251398985	30.305344908739084	20.58257936300403
54-55	21.158255321374405	28.285916008576955	30.531640604570892	20.024188065477748
56-57	21.624418178965534	26.731943935986614	30.311646880393283	21.33199100465457
58-59	22.235317190523507	28.198917420637	29.07674807802939	20.489017310810105
60-61	22.90790230636473	30.249411641650543	27.38742743580357	19.455258616181162
62-63	22.45855342293813	27.829062287537266	30.51320537628785	19.199178913236757
64-65	20.066262224779038	31.040296009622924	29.726217248051878	19.167224517546153
66-67	18.847157575440615	30.74690131269285	27.146462005125255	23.259479106741278
68-69	18.570969091574707	32.483081428795565	26.806626222477902	22.139323257151823
70-71	19.80330526646096	30.628758956121544	28.67376183254014	20.89417394487736
72-73	22.030045499712358	30.667407562365984	27.29329009989017	20.009256838031483
74-75	20.931593535902934	31.91896344333455	26.27365200564824	20.875791015114274
76-77	23.677998012656243	29.785471471157365	27.10551226400293	19.431018252183463
78-79	19.065007060300193	30.594895664452697	29.4854348621934	20.85466241305371
80-81	17.333063124313583	30.067125150358244	29.979368233878983	22.62044349144919
82-83	19.3710841483186	28.975053605982954	30.429004759165316	21.22485748653313
84-85	17.976622561581507	29.008472360232208	29.13443334553632	23.880471732649966
86	18.503530150096754	28.62664086606349	30.880602478949847	21.98922650488991
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	815.0
1	1021.5
2	4793.5
3	5211.0
4	2224.0
5	2799.0
6	3696.5
7	4180.0
8	4498.0
9	4896.0
10	4933.0
11	4601.5
12	4403.5
13	4531.0
14	4568.0
15	4492.5
16	4603.5
17	5091.0
18	5452.0
19	5459.0
20	5868.0
21	6330.0
22	6967.0
23	8538.0
24	9777.0
25	10878.5
26	13066.0
27	17709.0
28	22025.0
29	23089.0
30	27940.5
31	33805.5
32	36766.5
33	42555.5
34	49689.0
35	55906.0
36	58831.0
37	60027.5
38	64482.0
39	70813.5
40	77710.5
41	85368.5
42	93517.0
43	97831.0
44	100494.5
45	104151.0
46	106543.5
47	110708.0
48	110668.5
49	98502.0
50	89140.0
51	79544.0
52	65338.5
53	55347.5
54	47352.5
55	40891.0
56	33648.0
57	30253.0
58	26189.5
59	18986.0
60	14464.5
61	11102.5
62	7877.5
63	5505.0
64	4377.0
65	3404.5
66	1897.5
67	1060.5
68	660.5
69	457.5
70	289.0
71	226.0
72	184.5
73	138.0
74	87.5
75	28.5
76	16.5
77	12.5
78	7.5
79	8.0
80	5.0
81	2.0
82	2.5
83	2.5
84	1.0
85	1.5
86	3.0
87	1.5
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1912100.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.564295920257425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.03233880021402	14.671797247572158
2	8.142079962602104	3.023039636642879
3	2.7504641475287737	1.531812910583482
4	1.4503039887162883	1.0769548968343545
5	0.8517455944955797	0.7906028632495765
6	0.5548021847941285	0.6179707161434128
7	0.44534041101642713	0.5787201822750616
8	0.3386804440738781	0.5029891188953336
9	0.2970704926644782	0.49634140815000327
>10	3.394783263988457	15.18132855028044
>50	1.3030769119847414	17.59729354500302
>100	1.4322179894124871	42.273317028220795
>500	0.0062443114876135075	0.767504033287157
>1k	5.67664680692137E-4	0.12466803122047326
>5k	0.0	0.0
>10k+	2.838323403460685E-4	0.7656598316418837
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14531	0.7599497934208461	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0459703990377072E-4	0.0	0.0	0.028921081533392606	0.0
2	1.0459703990377072E-4	0.0	0.0	0.11212802677684222	0.0
3	1.0459703990377072E-4	0.0	0.0	0.1718529365618953	0.0
4	1.0459703990377072E-4	0.0	0.0	0.3064170283980963	0.0
5	1.0459703990377072E-4	0.0	0.0	0.6075519062810523	0.0
6	1.0459703990377072E-4	0.0	0.0	0.824381570001569	0.0
7	1.0459703990377072E-4	0.0	0.0	1.01914125830239	0.0
8	1.0459703990377072E-4	0.0	0.0	1.3468437843209038	0.0
9	1.0459703990377072E-4	0.0	0.0	1.459024109617698	0.0
10	1.0459703990377072E-4	0.0	0.0	1.708069661628576	0.0
11	1.0459703990377072E-4	0.0	0.0	2.044139950839391	0.0
12	1.0459703990377072E-4	0.0	0.0	2.372208566497568	0.0
13	1.0459703990377072E-4	0.0	0.0	2.460226975576591	0.0
14	1.0459703990377072E-4	0.0	0.0	2.5038439412164637	0.0
15	1.0459703990377072E-4	0.0	0.0	2.555514878928926	0.0
16	1.0459703990377072E-4	0.0	0.0	2.667538308665865	0.0
17	1.0459703990377072E-4	0.0	0.0	2.8228649129229644	0.0
18	1.0459703990377072E-4	0.0	0.0	3.012865435908164	0.0
19	1.0459703990377072E-4	0.0	0.0	3.158726008053972	0.0
20	1.0459703990377072E-4	0.0	0.0	3.2963757125673343	0.0
21	1.0459703990377072E-4	0.0	0.0	3.4707389780869202	0.0
22	1.0459703990377072E-4	0.0	0.0	3.6713038021024005	0.0
23	1.0459703990377072E-4	0.0	0.0	3.8720778201976884	0.0
24	1.0459703990377072E-4	0.0	0.0	4.015741854505517	0.0
25	1.0459703990377072E-4	0.0	0.0	4.140996809790283	0.0
26	1.0459703990377072E-4	0.0	0.0	4.262486271638513	0.0
27	1.0459703990377072E-4	0.0	0.0	4.3938601537576485	0.0
28	1.0459703990377072E-4	0.0	0.0	4.533183410909471	0.0
29	1.0459703990377072E-4	0.0	0.0	4.6950996286805085	0.0
30	1.0459703990377072E-4	0.0	0.0	4.884629464986141	0.0
31	1.0459703990377072E-4	0.0	0.0	5.05768526750693	0.0
32	1.0459703990377072E-4	0.0	0.0	5.212279692484703	0.0
33	1.0459703990377072E-4	0.0	0.0	5.368129281941321	0.0
34	1.0459703990377072E-4	0.0	0.0	5.5295225145128395	0.0
35	1.0459703990377072E-4	0.0	0.0	5.718110977459338	0.0
36	1.0459703990377072E-4	0.0	0.0	5.8897024214214735	0.0
37	1.0459703990377072E-4	0.0	0.0	6.076042048010041	0.0
38	1.0459703990377072E-4	0.0	0.0	6.272056900789708	0.0
39	1.0459703990377072E-4	0.0	0.0	6.552063176612102	0.0
40	1.0459703990377072E-4	0.0	0.0	6.757177971863396	0.0
41	1.5689555985565608E-4	0.0	0.0	6.971078918466607	0.0
42	1.5689555985565608E-4	0.0	0.0	7.180116102714293	0.0
43	1.5689555985565608E-4	0.0	0.0	7.378955075571361	0.0
44	1.5689555985565608E-4	0.0	0.0	7.574656137231316	0.0
45	1.5689555985565608E-4	0.0	0.0	7.774122692327807	0.0
46	1.5689555985565608E-4	0.0	0.0	7.993358087966111	0.0
47	1.5689555985565608E-4	0.0	0.0	8.214110140683019	0.0
48	2.0919407980754144E-4	0.0	0.0	8.429004759165316	0.0
49	2.0919407980754144E-4	0.0	0.0	8.658072276554574	0.0
50	2.0919407980754144E-4	0.0	0.0	8.882328330108258	0.0
51	2.0919407980754144E-4	0.0	0.0	9.115893520213378	0.0
52	2.0919407980754144E-4	0.0	0.0	9.370064327179541	0.0
53	2.0919407980754144E-4	0.0	0.0	9.611840384917107	0.0
54	2.0919407980754144E-4	0.0	0.0	9.849641755138329	0.0
55	2.0919407980754144E-4	0.0	0.0	10.089273573557868	0.0
56	2.614925997594268E-4	0.0	0.0	10.334187542492547	0.0
57	2.614925997594268E-4	0.0	0.0	10.580722765545735	0.0
58	2.614925997594268E-4	0.0	0.0	10.819308613566236	0.0
59	2.614925997594268E-4	0.0	0.0	11.067726583337691	0.0
60	2.614925997594268E-4	0.0	0.0	11.321845091783903	0.0
61	2.614925997594268E-4	0.0	0.0	11.58616181162073	0.0
62	2.614925997594268E-4	0.0	0.0	11.877882955912348	0.0
63	2.614925997594268E-4	0.0	0.0	12.18670571622823	0.0
64	2.614925997594268E-4	0.0	0.0	12.465561424611684	0.0
65	2.614925997594268E-4	0.0	0.0	12.739291878039852	0.0
66	2.614925997594268E-4	0.0	0.0	13.004916060875477	0.0
67	2.614925997594268E-4	0.0	0.0	13.277077558705088	0.0
68	2.614925997594268E-4	0.0	0.0	13.577375660268814	0.0
69	2.614925997594268E-4	0.0	0.0	13.954761780241618	0.0
70	2.614925997594268E-4	0.0	0.0	14.232623816745987	0.0
71	2.614925997594268E-4	0.0	0.0	14.513100779247948	0.0
72	2.614925997594268E-4	0.0	0.0	14.807593745097014	0.0
73	2.614925997594268E-4	0.0	0.0	15.099262590868678	0.0
74	2.614925997594268E-4	0.0	0.0	15.409288217143455	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAC	15	0.0013099249	80.0	9
CGTTTTT	7155	0.0	74.35359	1
CAGCGCG	120	0.0	70.0	1
ACGGGAT	1340	0.0	69.25373	5
TATCGGG	150	0.0	66.666664	3
CGTAAGG	340	0.0	65.882355	2
TACGGGA	1320	0.0	65.757576	4
CGGGTAG	140	0.0	65.71429	6
AGCGGGA	1730	0.0	65.433525	4
CGGGACG	105	0.0	64.76191	6
TAGGGGT	2875	0.0	64.13913	5
TCGGTCG	25	1.2357357E-4	64.0	2
ATAGGGA	3545	0.0	63.977432	4
TATCGCG	70	0.0	62.857143	1
TCGATAG	185	0.0	62.7027	1
TCGAGCG	115	0.0	62.608696	1
TAGGGAC	2655	0.0	62.52354	5
CCGATCC	1440	0.0	61.944447	80
AGGGATC	2770	0.0	61.805054	6
GTACGGG	745	0.0	61.744965	3
>>END_MODULE