FastQCFastQC Report
Thu 26 May 2016
SRR1547826_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547826_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3140674
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT516641.6449972203418757No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT261550.076.0391
TACGCGG2250.067.555562
CCGATCC21700.065.25345680
TAGCGGG17900.063.6871533
TAGGGAC36450.063.6488345
GGGCGAT18950.063.1134537
CGGGATC10950.062.831056
TAGGGAT55050.061.980025
TGCGGGA26050.061.8814
AAGGGAT57650.061.54385
AGGGATT67050.061.5063366
ACGGGAT19500.061.3333325
ATAGGGA47600.061.0084044
TTAGGGA52050.060.94144
GTAGGGA45850.060.3707774
TACGGGA15250.060.327874
TAAGGGA48500.059.711344
TTAGCGG7450.059.0604062
AGGGACT75200.059.0425576
TAACGGG9150.059.0163963
Produced by FastQC (version 0.11.5)