FastQCFastQC Report
Thu 26 May 2016
SRR1547825_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547825_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2103828
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108420.5153463115806045No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCG650.073.846151
CGTTTTT65750.073.794681
GGGCGAT18200.072.307697
CGTAAGG2200.070.909092
ACGTGCG700.068.571431
ACGGGAT10700.068.0373845
ACGTAGG4500.067.555562
TACGGGA10550.065.213274
ATTAGCG2150.065.116281
CGGGATC7400.064.3243266
TATAGCG2000.064.01
GGCGATT7250.064.08
AGGGATT50800.063.543316
TAGGGAT43300.063.46425
ATAGGGA36050.063.023584
TACGCGG1850.062.70272
TTAGGGA37150.062.341864
CGAGGGA15000.062.1333354
ACGGGAC9550.061.570685
GCGGGAT16900.061.5384645
Produced by FastQC (version 0.11.5)