##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547825_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2103828
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1205431242478	33.0	31.0	34.0	31.0	34.0
2	32.56017459602211	34.0	31.0	34.0	31.0	34.0
3	32.67986641493506	34.0	31.0	34.0	31.0	34.0
4	36.19758601938942	37.0	35.0	37.0	35.0	37.0
5	36.16519363750268	37.0	35.0	37.0	35.0	37.0
6	36.22413952091141	37.0	37.0	37.0	35.0	37.0
7	36.40793924218139	37.0	37.0	37.0	35.0	37.0
8	36.41674937304761	37.0	37.0	37.0	35.0	37.0
9	38.23196430506676	39.0	39.0	39.0	37.0	39.0
10-11	37.8186679234234	39.0	37.5	39.0	35.0	39.0
12-13	37.27099363636191	39.0	37.0	39.0	34.5	39.0
14-15	38.55350461159372	40.0	38.0	41.0	34.5	41.0
16-17	38.65660714659183	40.0	37.5	41.0	35.0	41.0
18-19	38.522054321931265	40.0	37.0	41.0	35.0	41.0
20-21	38.353671735522106	40.0	36.0	41.0	34.0	41.0
22-23	38.159382563593596	40.0	35.5	41.0	34.0	41.0
24-25	38.147548896582805	40.0	35.0	41.0	34.0	41.0
26-27	38.142779495281935	40.0	35.0	41.0	34.0	41.0
28-29	38.06193899881549	40.0	36.0	41.0	34.0	41.0
30-31	37.92810462642383	40.0	36.0	41.0	34.0	41.0
32-33	37.64993288424719	40.0	35.0	41.0	33.5	41.0
34-35	37.374029625996045	40.0	35.0	41.0	33.0	41.0
36-37	37.201042575723875	40.0	35.0	41.0	33.0	41.0
38-39	37.116375720828884	40.0	35.0	41.0	33.0	41.0
40-41	37.01862462140441	40.0	35.0	41.0	32.0	41.0
42-43	36.9618336194784	40.0	35.0	41.0	32.0	41.0
44-45	36.837582967809155	40.0	35.0	41.0	32.0	41.0
46-47	36.71946613506427	39.0	35.0	41.0	31.5	41.0
48-49	36.63890893171875	39.0	35.0	41.0	31.0	41.0
50-51	36.41878661183328	39.0	35.0	41.0	31.0	41.0
52-53	36.242614652908884	38.5	35.0	40.0	31.0	41.0
54-55	36.06986645296098	38.0	35.0	40.0	31.0	41.0
56-57	35.80323415222157	38.0	35.0	40.0	30.0	41.0
58-59	35.57597816931802	37.0	35.0	40.0	29.5	41.0
60-61	35.24203499525626	37.0	35.0	40.0	29.0	41.0
62-63	34.880370210872755	36.0	34.0	39.5	28.0	41.0
64-65	34.532671159429384	36.0	34.0	39.0	28.0	40.5
66-67	34.22282334867679	35.0	34.0	39.0	27.5	40.0
68-69	33.767961782046825	35.0	34.0	38.0	26.0	40.0
70-71	33.45367563317914	35.0	34.0	37.0	26.0	39.5
72-73	33.15865959574642	35.0	33.5	37.0	25.5	39.0
74-75	32.839549858638634	35.0	33.0	36.0	25.0	39.0
76-77	30.84726008970315	33.5	30.0	34.5	21.5	36.0
78-79	31.87251286702145	34.5	32.0	35.0	23.0	37.0
80-81	31.98816181741093	35.0	33.0	35.0	23.0	37.0
82-83	31.76905740393226	35.0	33.0	35.0	22.0	36.0
84-85	31.543360008517805	35.0	32.0	35.0	20.0	36.0
86	31.121004663879365	34.0	32.0	35.0	19.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	13.0
11	13.0
12	29.0
13	49.0
14	213.0
15	530.0
16	1517.0
17	3570.0
18	8474.0
19	14339.0
20	17102.0
21	16448.0
22	13813.0
23	11625.0
24	10873.0
25	11648.0
26	13709.0
27	16858.0
28	20907.0
29	27098.0
30	35012.0
31	45018.0
32	57081.0
33	77801.0
34	193699.0
35	187795.0
36	177162.0
37	341325.0
38	537009.0
39	262503.0
40	588.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.301893500799494	24.155254136745018	31.316628545679592	13.226223816775896
2	30.342499481896805	25.94717819137306	31.0640888893959	12.646233437334232
3	28.51811079612972	25.253680433951825	33.104845072886185	13.123363697032268
4	26.735503092458128	27.39525284386366	31.821565261038447	14.04767880263976
5	23.434757974511225	32.43402027161916	30.24776740303865	13.883454350830963
6	21.96206153735001	41.23203037510671	25.949935070737723	10.855973016805557
7	89.09654211275827	3.579094868972178	5.920826227239108	1.4035367910304455
8	90.35748169527167	2.4075162037961277	5.936131660953272	1.2988704399789335
9	86.05784313166286	5.629832857058656	6.338018126957147	1.974305884321342
10-11	36.52354184847811	30.255847911521283	20.715856999716706	12.504753240283902
12-13	26.885230161401026	25.264731717611895	30.170384651216736	17.679653469770344
14-15	17.226622138311686	31.769493513728307	33.77526584872908	17.228618499230926
16-17	21.73438132775113	27.174559897482116	32.03451042575724	19.05654834900952
18-19	23.042259158068052	29.000231958125855	30.025529653564835	17.931979230241254
20-21	26.31721794747479	27.646746787284894	29.488223371872607	16.547811893367708
22-23	21.666409991691335	25.19654648573933	31.881527387219865	21.255516135349467
24-25	19.655955715010922	28.307708614962817	33.0895158729706	18.946819797055653
26-27	18.878610798981665	33.26291407852733	29.37497742210865	18.48349770038235
28-29	17.747458442420196	28.88270809210639	34.529319887367215	18.840513578106194
30-31	20.917418153955552	28.797339896607514	30.86908720674884	19.41615474268809
32-33	23.198117906977185	28.473549168468143	29.478978319520415	18.84935460503425
34-35	20.082202537469794	28.408976399211344	30.588265770775934	20.92055529254293
36-37	21.302882174778546	29.828626674804216	29.791979192215333	19.076511958201905
38-39	19.873701652416454	30.824929604511397	29.003464161518906	20.297904581553244
40-41	20.382203297988237	28.02294198955428	29.269027696180487	22.325827016276996
42-43	20.41927857220267	27.630657068923885	28.80708403918952	23.14298031968393
44-45	19.786455927005438	30.529895980089627	28.718150913477718	20.965497179427214
46-47	20.86634458710503	29.597001275769692	28.908161693826685	20.628492443298597
48-49	20.470138243240417	27.686911667683862	30.890761982443433	20.952188106632292
50-51	19.299629057128247	29.83297588966398	29.970605962084353	20.89678909112342
52-53	21.17338014324365	26.87641290067439	30.60830067857258	21.341906277509377
54-55	21.05811406635904	27.890469182841944	30.430767153968862	20.62064959683016
56-57	21.44849769087587	26.95904798301002	29.813962928528376	21.778491397585732
58-59	22.055629072338615	27.906131109577398	29.32380879045245	20.714431027631537
60-61	22.91843724867242	29.35884017134481	28.01666771237953	19.706054867603246
62-63	21.90045954327065	27.592464783242736	29.975882058799485	20.531193614687133
64-65	20.072553459693474	30.172333479733133	29.918724344385566	19.836388716187827
66-67	18.566679405350627	29.67074779877443	27.810947472892273	23.95162532298268
68-69	19.29979542053818	31.19501689301597	26.986878204872262	22.51830948157359
70-71	19.297133605979198	30.148781174126405	29.111719208984766	21.442366010909637
72-73	21.50477605583726	29.814176824341153	27.92336160560654	20.75768551421504
74-75	20.482496667978562	31.36865276058689	26.72956154210325	21.419289029331296
76-77	22.56170656536561	29.407798546268992	27.46001574273182	20.570479145633577
78-79	19.141702648695617	29.73767342197176	29.685150116834645	21.43547381249798
80-81	18.032938053871327	29.24611707801208	29.43886097152429	23.282083896592308
82-83	19.063987170053824	29.165240694581495	29.891131784537517	21.87964035082716
84-85	17.92601391368496	28.617691180077458	28.878549006857973	24.577745899379607
86	18.733708268926925	28.68390381723221	29.906817477474394	22.67557043636647
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	603.0
1	844.5
2	3595.5
3	4011.0
4	2028.5
5	2573.0
6	3215.0
7	3424.0
8	3640.0
9	3888.5
10	3918.5
11	3894.0
12	3854.0
13	4017.5
14	4199.0
15	4138.0
16	4290.0
17	4540.5
18	4628.5
19	5223.5
20	5917.0
21	6066.0
22	6862.5
23	8315.5
24	10360.0
25	13126.0
26	16512.5
27	20478.0
28	25996.5
29	29558.0
30	30451.5
31	34900.0
32	40698.5
33	46755.0
34	52910.5
35	60090.5
36	64928.0
37	64588.5
38	69869.5
39	80396.0
40	89321.0
41	96089.0
42	104275.5
43	109713.0
44	111469.0
45	112590.5
46	112819.0
47	113962.0
48	111970.5
49	103732.5
50	97766.0
51	87591.0
52	71736.0
53	62972.0
54	55619.5
55	48799.0
56	43094.5
57	39942.0
58	37000.0
59	28658.0
60	18905.5
61	12923.5
62	9800.5
63	7195.5
64	6084.0
65	4842.0
66	2807.0
67	1645.5
68	1179.5
69	799.5
70	468.0
71	419.0
72	289.5
73	176.5
74	124.5
75	46.0
76	32.0
77	19.0
78	8.5
79	7.0
80	6.5
81	6.5
82	4.5
83	4.0
84	3.5
85	1.5
86	2.0
87	2.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	2.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2103828.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.127958191306075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.46609729034657	14.405580893056635
2	8.047961369752684	2.91786214472246
3	2.5675003865655186	1.396306189914657
4	1.2859547665057451	0.9324693697250764
5	0.7323057203249643	0.6637603740652616
6	0.5356129888004825	0.5825741920617381
7	0.390925434576823	0.4960675953748763
8	0.31180231662137015	0.4521871487731662
9	0.2525101798851608	0.41197445854536335
>10	3.497164347207381	15.38089435451027
>50	1.3889485818492997	18.36002763125784
>100	1.5184681071535042	42.87670211823317
>500	0.003693285874894983	0.43174302560838546
>1k	7.914184017632106E-4	0.17335716302950788
>5k	0.0	0.0
>10k+	2.638061339210702E-4	0.5184933411216344
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10842	0.5153463115806045	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.029137362940316415	0.0
2	0.0	0.0	0.0	0.08622377874997386	0.0
3	0.0	0.0	0.0	0.1333759223662771	0.0
4	0.0	0.0	0.0	0.22121580281277747	0.0
5	0.0	0.0	0.0	0.45046458170534853	0.0
6	0.0	0.0	0.0	0.6381700405166202	0.0
7	0.0	0.0	0.0	0.7655568801251813	0.0
8	0.0	0.0	0.0	1.0109191435801785	0.0
9	0.0	0.0	0.0	1.0874938445538322	0.0
10	0.0	0.0	0.0	1.2604167260821701	0.0
11	0.0	0.0	0.0	1.5973739298079501	0.0
12	0.0	0.0	0.0	1.8235806349188242	0.0
13	0.0	0.0	0.0	1.9101846728915102	0.0
14	0.0	0.0	0.0	1.9386090497892414	0.0
15	0.0	0.0	0.0	1.9850482073629594	0.0
16	0.0	0.0	0.0	2.0962264976034164	0.0
17	0.0	0.0	0.0	2.2497086263705968	0.0
18	0.0	0.0	0.0	2.426956956557285	0.0
19	0.0	0.0	0.0	2.5586217124213575	0.0
20	0.0	0.0	0.0	2.69223529680183	0.0
21	0.0	0.0	0.0	2.8610228592831732	0.0
22	0.0	0.0	0.0	3.0441652074218997	0.0
23	0.0	0.0	0.0	3.2453698686394516	0.0
24	0.0	0.0	0.0	3.3855904570145468	0.0
25	0.0	0.0	0.0	3.5090321071874695	0.0
26	0.0	0.0	0.0	3.630049604815603	0.0
27	0.0	0.0	0.0	3.7528733337516185	0.0
28	0.0	0.0	0.0	3.8926185980983234	0.0
29	0.0	0.0	0.0	4.044056833543427	0.0
30	0.0	0.0	0.0	4.223919445886261	0.0
31	0.0	0.0	0.0	4.399123882750871	0.0
32	0.0	0.0	0.0	4.553128867949281	0.0
33	0.0	0.0	0.0	4.712267352654305	0.0
34	0.0	0.0	0.0	4.878440632979502	0.0
35	0.0	0.0	0.0	5.064767652108443	0.0
36	0.0	0.0	0.0	5.24396481081153	0.0
37	4.753240283901536E-5	0.0	0.0	5.429246117078012	0.0
38	4.753240283901536E-5	0.0	0.0	5.618234950765937	0.0
39	4.753240283901536E-5	0.0	0.0	5.851856710719698	0.0
40	4.753240283901536E-5	0.0	0.0	6.054297214411064	0.0
41	4.753240283901536E-5	0.0	0.0	6.253077723083826	0.0
42	4.753240283901536E-5	0.0	0.0	6.466926003456557	0.0
43	4.753240283901536E-5	0.0	0.0	6.656247563964355	0.0
44	4.753240283901536E-5	0.0	0.0	6.850655091575927	0.0
45	4.753240283901536E-5	0.0	0.0	7.0470114477039	0.0
46	9.506480567803072E-5	0.0	0.0	7.260574533659596	0.0
47	9.506480567803072E-5	0.0	0.0	7.481980466083729	0.0
48	9.506480567803072E-5	0.0	0.0	7.687795770376666	0.0
49	9.506480567803072E-5	0.0	0.0	7.909867156440545	0.0
50	9.506480567803072E-5	0.0	0.0	8.131082959253323	0.0
51	9.506480567803072E-5	0.0	0.0	8.362755890690684	0.0
52	9.506480567803072E-5	0.0	0.0	8.605361274781018	0.0
53	9.506480567803072E-5	0.0	0.0	8.833659405616809	0.0
54	9.506480567803072E-5	0.0	0.0	9.075979595290109	0.0
55	9.506480567803072E-5	0.0	0.0	9.305038244571325	0.0
56	1.4259720851704608E-4	0.0	0.0	9.545076878908352	0.0
57	1.9012961135606143E-4	0.0	0.0	9.784925383634024	0.0
58	1.9012961135606143E-4	0.0	0.0	10.033900109704787	0.0
59	1.9012961135606143E-4	0.0	0.0	10.292666510760386	0.0
60	1.9012961135606143E-4	0.0	0.0	10.547250060366151	0.0
61	1.9012961135606143E-4	0.0	0.0	10.811054896122688	0.0
62	1.9012961135606143E-4	0.0	0.0	11.086267508560585	4.753240283901536E-5
63	1.9012961135606143E-4	0.0	0.0	11.370986601566289	4.753240283901536E-5
64	1.9012961135606143E-4	0.0	0.0	11.644440515099143	4.753240283901536E-5
65	1.9012961135606143E-4	0.0	0.0	11.909718855343687	4.753240283901536E-5
66	1.9012961135606143E-4	0.0	0.0	12.171764992195179	4.753240283901536E-5
67	1.9012961135606143E-4	0.0	0.0	12.426871398232175	4.753240283901536E-5
68	1.9012961135606143E-4	0.0	0.0	12.706219329717069	4.753240283901536E-5
69	1.9012961135606143E-4	0.0	0.0	13.002678926224007	4.753240283901536E-5
70	1.9012961135606143E-4	0.0	0.0	13.286304773964412	4.753240283901536E-5
71	1.9012961135606143E-4	0.0	0.0	13.560424141137013	4.753240283901536E-5
72	1.9012961135606143E-4	0.0	0.0	13.842719081597926	4.753240283901536E-5
73	1.9012961135606143E-4	0.0	0.0	14.128626484674603	4.753240283901536E-5
74	1.9012961135606143E-4	0.0	0.0	14.42803309015756	4.753240283901536E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGCG	65	0.0	73.84615	1
CGTTTTT	6575	0.0	73.79468	1
GGGCGAT	1820	0.0	72.30769	7
CGTAAGG	220	0.0	70.90909	2
ACGTGCG	70	0.0	68.57143	1
ACGGGAT	1070	0.0	68.037384	5
ACGTAGG	450	0.0	67.55556	2
TACGGGA	1055	0.0	65.21327	4
ATTAGCG	215	0.0	65.11628	1
CGGGATC	740	0.0	64.324326	6
TATAGCG	200	0.0	64.0	1
GGCGATT	725	0.0	64.0	8
AGGGATT	5080	0.0	63.54331	6
TAGGGAT	4330	0.0	63.4642	5
ATAGGGA	3605	0.0	63.02358	4
TACGCGG	185	0.0	62.7027	2
TTAGGGA	3715	0.0	62.34186	4
CGAGGGA	1500	0.0	62.133335	4
ACGGGAC	955	0.0	61.57068	5
GCGGGAT	1690	0.0	61.538464	5
>>END_MODULE