FastQCFastQC Report
Thu 26 May 2016
SRR1547824_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547824_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4367780
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT731151.6739625164271095No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT425600.076.85151
TACGGGA19200.070.000014
TATCGCG1750.066.285711
CCGATCC29100.064.46735480
GCGGGAT30700.062.93165
TAGGGTA25700.062.8793755
CGAGGGA33100.062.4773454
ACGGGAT25150.061.23265
GCGAGGG34150.060.9077643
TAAGGGA60950.059.721084
GTACGGG17200.059.534883
TAGGGAT75400.059.4164435
TAGGGAC47250.059.343925
ATTAGCG3850.059.2207761
TAGCGGG29600.059.1891943
GTAGGGA69950.059.1851354
CGATAGG6900.059.1304322
AGGGATT82200.058.9294436
CGGGATA12750.058.6666686
GGGCGAT25150.058.3697787
Produced by FastQC (version 0.11.5)