##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547824_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4367780
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06011818360815	33.0	31.0	34.0	31.0	34.0
2	32.49626812705768	34.0	31.0	34.0	31.0	34.0
3	32.52966541355105	34.0	31.0	34.0	31.0	34.0
4	36.11159673793094	37.0	35.0	37.0	35.0	37.0
5	36.08898937217534	37.0	35.0	37.0	35.0	37.0
6	36.14785589017762	37.0	36.0	37.0	35.0	37.0
7	36.36180462385926	37.0	37.0	37.0	35.0	37.0
8	36.36663797169271	37.0	37.0	37.0	35.0	37.0
9	38.153721570225606	39.0	39.0	39.0	37.0	39.0
10-11	37.75867225455495	39.0	37.5	39.0	35.0	39.0
12-13	37.35889227479406	39.0	37.0	39.0	34.5	39.0
14-15	38.638223994798274	40.0	38.0	41.0	34.5	41.0
16-17	38.7354906840546	40.0	38.0	41.0	35.0	41.0
18-19	38.429257425969254	40.0	37.5	41.0	34.5	41.0
20-21	38.11016969719171	40.0	35.0	41.0	34.0	41.0
22-23	37.93198890511884	40.0	35.0	41.0	34.0	41.0
24-25	37.880478412374245	40.0	35.0	41.0	34.0	41.0
26-27	37.867148757492366	40.0	35.0	41.0	34.0	41.0
28-29	37.72845461538814	40.0	35.0	41.0	33.5	41.0
30-31	37.438126118989516	40.0	35.0	41.0	33.0	41.0
32-33	36.82105302922766	40.0	35.0	41.0	31.0	41.0
34-35	36.218661081831044	40.0	35.0	41.0	29.0	41.0
36-37	35.93755088397309	40.0	35.0	41.0	26.5	41.0
38-39	35.79538793162659	40.0	35.0	41.0	25.0	41.0
40-41	35.664079234759996	39.0	35.0	41.0	24.0	41.0
42-43	35.5822242649584	39.0	35.0	41.0	23.5	41.0
44-45	35.438576004285935	39.0	35.0	41.0	23.0	41.0
46-47	35.32365332045112	39.0	35.0	41.0	23.0	41.0
48-49	35.20574158954892	39.0	35.0	41.0	23.0	41.0
50-51	34.98555353062655	38.5	35.0	40.5	23.0	41.0
52-53	34.77699288883598	38.0	34.5	40.0	22.5	41.0
54-55	34.560475802352684	38.0	34.0	40.0	22.0	41.0
56-57	34.281047122336744	37.0	34.0	40.0	20.5	41.0
58-59	34.02700502314677	37.0	34.0	40.0	20.0	41.0
60-61	33.67427995457646	36.0	34.0	39.5	19.5	41.0
62-63	33.328544020074276	36.0	33.0	39.0	18.0	41.0
64-65	32.97416307597911	35.0	33.0	39.0	18.0	40.0
66-67	32.6316984829822	35.0	33.0	38.0	17.5	40.0
68-69	32.14821190627733	35.0	32.5	37.5	14.0	40.0
70-71	31.85124193526231	35.0	32.0	37.0	12.5	39.0
72-73	31.588736612192005	35.0	32.0	36.5	11.0	39.0
74-75	31.29017372669869	35.0	32.0	36.0	10.5	38.5
76-77	29.397601642024092	33.0	29.0	34.5	10.0	36.0
78-79	30.474811918182695	34.0	30.5	35.0	10.0	37.0
80-81	30.55921864196457	34.5	31.0	35.0	10.0	36.5
82-83	30.369122071166586	34.0	31.0	35.0	10.0	36.0
84-85	30.15826083273425	34.0	31.0	35.0	10.0	36.0
86	29.74861920701134	34.0	30.0	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	17.0
10	17.0
11	33.0
12	76.0
13	215.0
14	770.0
15	2598.0
16	7024.0
17	17952.0
18	42928.0
19	70050.0
20	80586.0
21	74512.0
22	59550.0
23	45357.0
24	37261.0
25	35173.0
26	37239.0
27	42148.0
28	50148.0
29	62251.0
30	78441.0
31	97566.0
32	118415.0
33	150170.0
34	327802.0
35	334933.0
36	368289.0
37	699359.0
38	1063389.0
39	462460.0
40	1041.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.720585743787463	23.141779118911668	28.96666040872022	19.170974728580653
2	36.48439252892774	23.22005687099625	28.633516340108706	11.662034259967307
3	27.32346867287272	23.670560330419573	36.89439486421019	12.111576132497516
4	25.430905402744642	25.639203439733688	35.95664158909103	12.973249568430646
5	22.79670679384035	30.104492442384917	34.12346775707567	12.975333006699055
6	21.216727948752	38.27159334948188	30.621597241619313	9.890081460146801
7	83.03163162979821	3.1337658948023943	12.245740399012771	1.5888620763866312
8	84.00757364152956	2.5861879490267365	12.049942991634193	1.356295417809505
9	79.16181675817006	5.222492891125468	13.3250072119017	2.2906831388027786
10-11	31.05481503189263	27.798858916886836	27.42187106493459	13.724454986285938
12-13	26.26507058505694	22.572989024172465	33.567132502094886	17.59480788867571
14-15	17.931512118284346	28.282319622325303	36.56301370490272	17.223154554487635
16-17	23.02083667217671	26.139228624152317	34.152624445370414	16.687310258300556
18-19	22.614142653705084	27.425522805635815	33.14200806817193	16.818326472487165
20-21	24.420483632417383	26.328329265668142	34.02300482167142	15.228182280243052
22-23	21.833013567533165	22.79931681540737	36.600412566566995	18.767257050492468
24-25	19.715954100252304	27.413022176025347	35.77835880012272	17.09266492359963
26-27	19.281591563677658	29.636840683367755	33.22763509151102	17.85393266144357
28-29	18.130915476512097	27.258824849236913	36.37055895672401	18.239700717526983
30-31	20.766190146939635	26.492199698702773	34.45467720443795	18.28693294991964
32-33	22.489949127474368	26.987508528359943	32.509169417873615	18.013372926292075
34-35	20.578520438300462	27.92926841553375	32.19973304516253	19.292478101003255
36-37	21.546758307423907	28.833675230895327	31.088459125688566	18.531107335992196
38-39	20.473570097395015	29.267900397913817	30.308589260448098	19.94994024424307
40-41	20.750049224090958	27.68109886486957	30.965650742482453	20.603201168557025
42-43	20.88293137474873	28.04852808520576	30.1684265233139	20.90011401673161
44-45	20.503963111695185	28.79296805242022	30.51395674690575	20.189112088978842
46-47	20.697516816323166	28.68225963761911	30.566901263342018	20.053322282715705
48-49	20.76021457124672	28.044315418816883	31.172082843000332	20.023387166936065
50-51	19.75761141815751	28.692905778221427	31.465756059142176	20.083726744478888
52-53	20.993697026864908	27.415952726556743	31.00939149865607	20.580958747922285
54-55	20.846471205051536	27.651552962832376	31.317076409526123	20.184899422589968
56-57	20.89745820531254	27.32143102445636	31.165489104304704	20.615621665926398
58-59	21.497992114987476	28.284380165667685	29.990601632866127	20.227026086478713
60-61	21.827060886766276	29.297068533671567	29.053020069692153	19.82285050987
62-63	21.619712989207333	28.36739716743975	30.0101081098407	20.00278173351222
64-65	21.24762465142475	29.65986153148739	29.483147044951895	19.609366772135957
66-67	20.421358218591596	29.695245639661337	28.543573623213625	21.339822518533445
68-69	19.832386246560038	30.75700928160301	28.535812243290643	20.87479222854631
70-71	20.300644721116907	29.85982581540279	29.109284350402266	20.730245113078038
72-73	21.526942748947977	29.860650032739745	28.589661109304952	20.022746109007322
74-75	20.853442710026606	30.26634812192922	27.713781371772388	21.16642779627179
76-77	21.752583692402087	29.48760010806405	28.49858509357156	20.261231105962295
78-79	20.10435507282876	29.589677135753174	29.0655893840807	21.240378407337364
80-81	19.37778459537797	29.424089583266554	28.989841521322045	22.208284300033426
82-83	20.54969572643311	29.315682108531107	29.0133088204992	21.12131334453658
84-85	19.708101140625214	28.988238876500187	28.774411714875747	22.529248267998845
86	19.81336056303202	29.255067791875966	29.10602182344349	21.825549821648526
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1716.0
1	1862.5
2	18015.0
3	21986.5
4	10568.0
5	12359.5
6	14403.5
7	15272.0
8	15756.0
9	16636.5
10	17413.0
11	18042.5
12	18674.5
13	19349.0
14	19641.0
15	19793.0
16	20343.0
17	20890.5
18	20871.0
19	21454.5
20	22941.5
21	23676.0
22	23940.0
23	25027.0
24	28471.0
25	32886.0
26	36962.0
27	43400.5
28	51462.0
29	55367.0
30	62303.0
31	73088.0
32	79807.0
33	88116.0
34	100129.0
35	113580.0
36	120457.0
37	122686.0
38	132843.5
39	144662.0
40	156821.0
41	174223.5
42	188406.0
43	193455.0
44	197819.5
45	202188.5
46	205672.0
47	211700.5
48	212486.0
49	205837.0
50	200952.0
51	184723.0
52	159601.0
53	141443.0
54	125784.0
55	111093.0
56	96885.0
57	90974.0
58	80353.5
59	61362.0
60	46624.0
61	36063.5
62	29619.0
63	21963.0
64	16558.0
65	14535.0
66	10471.0
67	6951.5
68	4626.5
69	3126.0
70	2316.5
71	2161.0
72	1595.0
73	843.5
74	458.5
75	207.0
76	128.0
77	79.0
78	52.5
79	48.0
80	35.0
81	28.5
82	23.5
83	13.0
84	11.5
85	7.0
86	5.0
87	5.0
88	5.0
89	5.5
90	3.0
91	0.5
92	2.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	4367780.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.317098797924643
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.00515239635814	15.454674331834681
2	7.270076162249777	2.8087355898923154
3	2.4017003377948507	1.3918164812457636
4	1.2550847482404934	0.9697838432611997
5	0.7988586831829224	0.7715816054312248
6	0.6197641980064739	0.7183227746584553
7	0.4644959402350833	0.6280899778129203
8	0.37070498292927406	0.5728755824102204
9	0.2974591310798651	0.5171442681073115
>10	3.88263607511979	17.906399579984303
>50	1.4256578905976107	19.81197505586561
>100	1.2020301894943657	35.40928829838224
>500	0.0037312680387212205	0.5046106656186222
>1k	0.0025276331875207537	0.834724577541067
>5k	0.0	0.0
>10k+	1.203634851200359E-4	1.6999773679539598
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	73115	1.6739625164271095	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.289492602649401E-5	0.0	0.0	0.01918594801020198	0.0
2	2.289492602649401E-5	0.0	0.0	0.05796995269908283	0.0
3	2.289492602649401E-5	0.0	0.0	0.10923169207240291	0.0
4	2.289492602649401E-5	0.0	0.0	0.1753293435108911	0.0
5	2.289492602649401E-5	0.0	0.0	0.35200948765734535	0.0
6	2.289492602649401E-5	0.0	0.0	0.6075626519650715	0.0
7	2.289492602649401E-5	0.0	0.0	0.787539665459341	0.0
8	2.289492602649401E-5	0.0	0.0	1.024227410721236	0.0
9	2.289492602649401E-5	0.0	0.0	1.1558732353735766	0.0
10	2.289492602649401E-5	0.0	0.0	1.3342476040459914	0.0
11	2.289492602649401E-5	0.0	0.0	1.5638608171656998	0.0
12	2.289492602649401E-5	0.0	0.0	1.7350461790657954	0.0
13	2.289492602649401E-5	0.0	0.0	1.813163666668193	0.0
14	2.289492602649401E-5	0.0	0.0	1.8551758559268094	0.0
15	2.289492602649401E-5	0.0	0.0	1.9015838709825128	0.0
16	2.289492602649401E-5	0.0	0.0	1.9870277349133885	0.0
17	2.289492602649401E-5	0.0	0.0	2.0940386191612217	0.0
18	2.289492602649401E-5	0.0	0.0	2.2201438717151505	0.0
19	2.289492602649401E-5	0.0	0.0	2.307144590615828	0.0
20	2.289492602649401E-5	0.0	0.0	2.401700635105248	0.0
21	2.289492602649401E-5	0.0	0.0	2.516175265237718	0.0
22	2.289492602649401E-5	0.0	0.0	2.646859502996946	0.0
23	2.289492602649401E-5	0.0	0.0	2.781641932514916	0.0
24	4.578985205298802E-5	0.0	0.0	2.886157269825861	0.0
25	4.578985205298802E-5	0.0	0.0	2.983323335882302	0.0
26	4.578985205298802E-5	0.0	0.0	3.0754983080649665	0.0
27	4.578985205298802E-5	0.0	0.0	3.1780904715896865	0.0
28	4.578985205298802E-5	0.0	0.0	3.2815755372294393	0.0
29	4.578985205298802E-5	0.0	0.0	3.4025981162054864	0.0
30	4.578985205298802E-5	0.0	0.0	3.5371973863152446	0.0
31	4.578985205298802E-5	0.0	0.0	3.6544194075708942	0.0
32	4.578985205298802E-5	0.0	0.0	3.7678408711061455	0.0
33	6.868477807948202E-5	0.0	0.0	3.883299983057755	0.0
34	6.868477807948202E-5	0.0	0.0	4.007298902417246	0.0
35	6.868477807948202E-5	0.0	0.0	4.147736378663761	0.0
36	6.868477807948202E-5	0.0	0.0	4.277596399086034	0.0
37	6.868477807948202E-5	0.0	0.0	4.412768042346455	0.0
38	6.868477807948202E-5	0.0	0.0	4.565500093869197	0.0
39	6.868477807948202E-5	0.0	0.0	4.780597923888108	0.0
40	6.868477807948202E-5	0.0	0.0	4.944777438424096	0.0
41	9.157970410597604E-5	0.0	0.0	5.105980612576641	0.0
42	9.157970410597604E-5	0.0	0.0	5.270915659671504	0.0
43	9.157970410597604E-5	0.0	0.0	5.4283869608817295	0.0
44	9.157970410597604E-5	0.0	0.0	5.5753952808978475	0.0
45	9.157970410597604E-5	0.0	0.0	5.724372564552244	0.0
46	9.157970410597604E-5	0.0	0.0	5.8904294630224046	0.0
47	1.1447463013247004E-4	0.0	0.0	6.054929506522765	0.0
48	1.3736955615896405E-4	0.0	0.0	6.220391136916237	0.0
49	1.3736955615896405E-4	0.0	0.0	6.386081716569974	0.0
50	1.3736955615896405E-4	0.0	0.0	6.557518922656361	0.0
51	1.3736955615896405E-4	0.0	0.0	6.731131146715265	0.0
52	1.3736955615896405E-4	0.0	0.0	6.924089583266556	0.0
53	1.6026448218545806E-4	0.0	0.0	7.101799999084203	0.0
54	1.6026448218545806E-4	0.0	0.0	7.280311737312776	0.0
55	1.6026448218545806E-4	0.0	0.0	7.457632939387973	0.0
56	1.6026448218545806E-4	0.0	0.0	7.637838902142507	0.0
57	1.6026448218545806E-4	0.0	0.0	7.8288970598336	0.0
58	1.8315940821195207E-4	0.0	0.0	8.008896968253895	0.0
59	2.060543342384461E-4	0.0	0.0	8.197276419599888	0.0
60	2.060543342384461E-4	0.0	0.0	8.389983011964889	0.0
61	2.060543342384461E-4	0.0	0.0	8.5924657377432	0.0
62	2.060543342384461E-4	0.0	0.0	8.830916392309136	0.0
63	2.2894926026494007E-4	0.0	0.0	9.056019305001625	0.0
64	2.2894926026494007E-4	0.0	0.0	9.267362367152192	0.0
65	2.2894926026494007E-4	0.0	0.0	9.475912248327525	0.0
66	2.747391123179281E-4	0.0	0.0	9.692910357206635	0.0
67	2.976340383444221E-4	0.0	0.0	9.903864205614752	0.0
68	3.205289643709161E-4	0.0	0.0	10.136774288082275	0.0
69	3.4342389039741014E-4	0.0	0.0	10.428478540585836	0.0
70	3.4342389039741014E-4	0.0	0.0	10.651246170823622	0.0
71	3.6631881642390415E-4	0.0	0.0	10.872594315647767	0.0
72	3.6631881642390415E-4	0.0	0.0	11.103627014181118	0.0
73	3.6631881642390415E-4	0.0	0.0	11.326188590084666	0.0
74	4.121086684768922E-4	0.0	0.0	11.566493733658746	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	42560	0.0	76.8515	1
TACGGGA	1920	0.0	70.00001	4
TATCGCG	175	0.0	66.28571	1
CCGATCC	2910	0.0	64.467354	80
GCGGGAT	3070	0.0	62.9316	5
TAGGGTA	2570	0.0	62.879375	5
CGAGGGA	3310	0.0	62.477345	4
ACGGGAT	2515	0.0	61.2326	5
GCGAGGG	3415	0.0	60.907764	3
TAAGGGA	6095	0.0	59.72108	4
GTACGGG	1720	0.0	59.53488	3
TAGGGAT	7540	0.0	59.416443	5
TAGGGAC	4725	0.0	59.34392	5
ATTAGCG	385	0.0	59.220776	1
TAGCGGG	2960	0.0	59.189194	3
GTAGGGA	6995	0.0	59.185135	4
CGATAGG	690	0.0	59.130432	2
AGGGATT	8220	0.0	58.929443	6
CGGGATA	1275	0.0	58.666668	6
GGGCGAT	2515	0.0	58.369778	7
>>END_MODULE