FastQCFastQC Report
Thu 26 May 2016
SRR1547823_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547823_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3137358
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349951.115428969215499No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT188550.075.926811
TAACGCG800.075.000011
CGAGGGA26700.068.1647954
CCGATCC16750.068.059780
TAGGGAT72150.065.918235
TACGGGA19850.065.088164
GCGGGAT25050.064.6706545
ACGGGAT23050.064.381785
AGGGATT72150.063.756066
CGGGACT14150.063.604246
CGGGATC14550.063.5051576
ACGGGAC14250.063.1578945
TAGGGAC43300.062.8175545
CGTAAGG5900.062.372882
ATAGGGA55950.062.12694
TAAGGGA56900.062.073814
GTACGGG12650.061.976283
GCGTAAG2150.061.3953481
GCGGGGT20200.061.1881185
CGGGATT20100.061.0945246
Produced by FastQC (version 0.11.5)