##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547823_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3137358
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18748290759295	33.0	31.0	34.0	31.0	34.0
2	32.63231068943997	34.0	31.0	34.0	31.0	34.0
3	32.71891062479959	34.0	31.0	34.0	31.0	34.0
4	36.235647318539996	37.0	37.0	37.0	35.0	37.0
5	36.2206911037886	37.0	37.0	37.0	35.0	37.0
6	36.282210382111316	37.0	37.0	37.0	35.0	37.0
7	36.45522315272915	37.0	37.0	37.0	35.0	37.0
8	36.46276357368206	37.0	37.0	37.0	35.0	37.0
9	38.291889545279815	39.0	39.0	39.0	37.0	39.0
10-11	37.91855503898503	39.0	38.0	39.0	35.0	39.0
12-13	37.42324178496684	39.0	37.0	39.0	35.0	39.0
14-15	38.69695536817922	40.0	38.0	41.0	35.0	41.0
16-17	38.81081183594604	40.5	38.0	41.0	35.0	41.0
18-19	38.59445798024963	40.0	37.5	41.0	35.0	41.0
20-21	38.384212608188164	40.0	36.0	41.0	35.0	41.0
22-23	38.22481129026397	40.0	35.0	41.0	34.0	41.0
24-25	38.20842696306892	40.0	35.0	41.0	34.0	41.0
26-27	38.20228039006068	40.0	35.0	41.0	34.5	41.0
28-29	38.096649473856665	40.0	36.0	41.0	34.0	41.0
30-31	37.907684108730976	40.0	35.5	41.0	34.0	41.0
32-33	37.505811099657734	40.0	35.0	41.0	33.0	41.0
34-35	37.09858820702004	40.0	35.0	41.0	32.5	41.0
36-37	36.88010214326832	40.0	35.0	41.0	31.5	41.0
38-39	36.780496519683126	40.0	35.0	41.0	31.0	41.0
40-41	36.662346471139095	40.0	35.0	41.0	31.0	41.0
42-43	36.615002176990956	40.0	35.0	41.0	31.0	41.0
44-45	36.49010983126567	40.0	35.0	41.0	31.0	41.0
46-47	36.38924933016889	39.0	35.0	41.0	30.0	41.0
48-49	36.297856349195726	39.0	35.0	41.0	30.0	41.0
50-51	36.07579259364089	39.0	35.0	41.0	29.5	41.0
52-53	35.89471555366012	39.0	35.0	40.0	29.0	41.0
54-55	35.71832860642617	38.0	35.0	40.0	29.0	41.0
56-57	35.45435522500142	38.0	35.0	40.0	28.5	41.0
58-59	35.21051518506973	37.0	35.0	40.0	28.0	41.0
60-61	34.87804914198507	37.0	34.5	40.0	27.0	41.0
62-63	34.52236786493604	36.0	34.0	39.5	26.0	41.0
64-65	34.196677108573525	36.0	34.0	39.0	26.0	40.5
66-67	33.880723844712655	35.0	34.0	39.0	25.5	40.0
68-69	33.39681445343503	35.0	34.0	38.0	24.0	40.0
70-71	33.107619213363606	35.0	33.5	37.0	24.0	39.5
72-73	32.849122892573945	35.0	33.0	37.0	23.5	39.0
74-75	32.538939292232506	35.0	33.0	36.0	23.0	39.0
76-77	30.590862120293572	33.5	30.0	34.5	19.5	36.0
78-79	31.660305741327576	34.5	32.0	35.0	20.0	37.0
80-81	31.761056277288088	35.0	33.0	35.0	20.0	37.0
82-83	31.578097558518984	35.0	33.0	35.0	19.5	36.5
84-85	31.34171793591933	35.0	32.0	35.0	19.0	36.0
86	30.909479249738155	34.0	31.0	35.0	18.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	19.0
11	23.0
12	34.0
13	74.0
14	295.0
15	891.0
16	2635.0
17	7595.0
18	18559.0
19	31639.0
20	36518.0
21	34471.0
22	27486.0
23	21179.0
24	17928.0
25	17971.0
26	19639.0
27	23965.0
28	29272.0
29	36522.0
30	47731.0
31	61423.0
32	79224.0
33	108280.0
34	266325.0
35	260221.0
36	257933.0
37	511070.0
38	817182.0
39	400350.0
40	897.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.17382778758433	23.500473965674303	30.54487246912848	15.780825777612883
2	31.97091310586806	24.84667035129558	30.58917726316219	12.59323927967417
3	27.830167931106363	24.44228551539225	35.21979321454548	12.507753338955899
4	26.104002157229107	26.57646975576265	34.0220338259134	13.297494261094844
5	22.707290656660795	31.767748532363854	32.05684528192192	13.468115529053426
6	21.330718394266768	39.78908368123753	28.92800247851855	9.952195445977157
7	87.77187684669713	2.940913979214358	8.199446795679677	1.0877623784088397
8	89.04514562890176	2.0850983534553595	7.770295898651031	1.0994601189918396
9	84.75554909576783	5.261752085672085	8.466040534742927	1.516658283817148
10-11	34.47701856147752	31.68471688599133	22.61291188318324	11.225352669347902
12-13	26.79230422540239	23.997165768139944	31.64960772726606	17.5609222791916
14-15	17.17668178129496	30.409471918729068	35.25603389858601	17.15781240138996
16-17	23.458862520630415	28.056967040420634	31.873362874112548	16.6108075648364
18-19	23.228636961417855	28.69666133096701	31.101041067037933	16.973660640577197
20-21	25.73917289643069	27.927861595648313	31.53291718700894	14.800048320912055
22-23	21.597583061926628	24.178863234606954	34.886806032336764	19.336747671129658
24-25	20.389129962216618	27.828462674645355	34.05805139228612	17.724355970851907
26-27	18.724018744434012	31.961016243603694	31.09222791916001	18.222737092802287
28-29	18.004145526267642	28.637423590167266	35.27005843770459	18.088372445860497
30-31	21.46323435196111	27.372585468410044	33.11623984256818	18.047940337060673
32-33	23.49970899081329	28.173514147891314	30.524951248789588	17.80182561250581
34-35	20.137580728753303	28.588481136038666	31.914145596390338	19.359792538817693
36-37	21.613440353316392	30.419336907040893	30.110621739693077	17.85660099994964
38-39	21.017636495420668	30.531453535108206	29.09830500695171	19.35260496251942
40-41	21.053494692030682	28.22706238816227	29.824776133294318	20.89466678651273
42-43	20.839971083950253	28.032599403702097	29.50528756998723	21.62214194236042
44-45	20.301763458298353	29.543759430705705	29.588574207980088	20.56590290301585
46-47	20.619562702120703	29.811213766487597	29.29345328139154	20.27577025000016
48-49	20.570429004276846	27.952452350034644	31.533076556771654	19.944042088916852
50-51	19.447732773881718	29.686506927166107	30.602691819040096	20.26306847991208
52-53	20.930461235217656	27.495379870579	30.93308127411663	20.64107762008671
54-55	20.567273482975164	27.807601172706462	31.436944715904275	20.1881806284141
56-57	21.02821864766469	27.639322640259735	30.279760868858446	21.05269784321713
58-59	21.69320491955333	29.371273536523407	29.578151425498778	19.357370118424484
60-61	22.77682368413168	29.43460708022483	28.353825097422735	19.434744138220758
62-63	21.25245190379931	27.709668453520447	30.944014039838613	20.093865602841625
64-65	20.16537481537013	30.09976865885245	29.858243783463667	19.876612742313757
66-67	20.51460177639912	29.76781738010135	28.31216584145004	21.405415002049494
68-69	19.72119534971782	30.760722875744495	28.402655992717442	21.115425781820246
70-71	19.808004059466597	29.836712928521386	29.911329851422757	20.44395316058926
72-73	20.982654832505567	30.952396889357225	28.135153846006734	19.929794432130475
74-75	20.375137297050575	31.47125702581599	27.589009606171818	20.56459607096162
76-77	21.82667709582394	28.97308818438954	28.632371568689326	20.567863151097196
78-79	19.60721409542679	29.609132907369833	29.30084803838134	21.48280495882204
80-81	18.89514999563327	29.38140945343184	29.513017003478726	22.21042354745617
82-83	19.667248685040086	29.539695501756576	29.74678057142347	21.046275241779867
84-85	18.747796713030517	29.07133964310098	29.121397685568557	23.059465958299945
86	19.56885379354221	29.29397920160849	29.478433764970397	21.658733239878906
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1105.0
1	1256.5
2	9759.0
3	11505.0
4	5349.0
5	6646.0
6	8051.5
7	8609.0
8	9186.0
9	9822.0
10	9923.0
11	10095.0
12	10141.0
13	10212.0
14	10367.0
15	10329.0
16	10107.0
17	9849.5
18	9961.5
19	10727.0
20	12046.5
21	12786.0
22	13379.5
23	15626.0
24	18717.5
25	22200.5
26	26728.0
27	32831.5
28	40127.0
29	43802.0
30	49842.5
31	56877.5
32	64042.5
33	73633.5
34	81162.5
35	90584.0
36	95897.0
37	97654.5
38	105296.0
39	114356.0
40	123903.5
41	135261.0
42	140737.0
43	141227.0
44	145285.0
45	147842.5
46	151320.0
47	158661.0
48	157052.0
49	144629.0
50	136178.0
51	126304.0
52	109415.0
53	95858.0
54	85444.0
55	79729.0
56	71295.5
57	64705.0
58	57284.0
59	42428.0
60	29409.5
61	20995.5
62	16605.0
63	11917.0
64	8789.0
65	6883.5
66	3820.5
67	2345.5
68	1584.0
69	984.0
70	666.5
71	505.0
72	412.5
73	263.5
74	137.5
75	75.5
76	73.0
77	44.5
78	30.5
79	35.0
80	29.5
81	18.0
82	11.5
83	9.0
84	6.5
85	4.0
86	2.0
87	1.0
88	0.5
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	3137358.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.25470897853756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.94803319261443	13.622253371664856
2	7.720361935584124	2.6642519681496606
3	2.5715833173738285	1.331157652660428
4	1.330125024540376	0.9180368081405728
5	0.8734960310078232	0.7535959904473805
6	0.6034219598173773	0.624712218474458
7	0.44747164674316464	0.5404695128490189
8	0.36843165621391477	0.508574480516136
9	0.30083886257616493	0.46717983208673824
>10	3.832828655179864	16.205075889950805
>50	1.4848217685368317	18.530529495148397
>100	1.5142787083653313	42.11891724400489
>500	0.0031836132434006157	0.36277950400557607
>1k	9.363568362942988E-4	0.22166806782562143
>5k	0.0	0.0
>10k+	1.8727136725885975E-4	1.130797964075455
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34995	1.115428969215499	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.187395254223458E-5	0.0	0.0	0.018518766427038293	0.0
2	3.187395254223458E-5	0.0	0.0	0.05683125738280426	0.0
3	3.187395254223458E-5	0.0	0.0	0.08841834435215873	0.0
4	3.187395254223458E-5	0.0	0.0	0.1474807784129194	0.0
5	6.374790508446916E-5	0.0	0.0	0.2958859014495636	0.0
6	6.374790508446916E-5	0.0	0.0	0.4560525129742924	0.0
7	6.374790508446916E-5	0.0	0.0	0.5669738678212687	0.0
8	6.374790508446916E-5	0.0	0.0	0.7457548676306625	0.0
9	6.374790508446916E-5	0.0	0.0	0.8339819682675678	0.0
10	6.374790508446916E-5	0.0	0.0	0.9653345266941166	0.0
11	6.374790508446916E-5	0.0	0.0	1.1600843767271698	0.0
12	6.374790508446916E-5	0.0	0.0	1.3096688360078768	0.0
13	6.374790508446916E-5	0.0	0.0	1.36720132034661	0.0
14	6.374790508446916E-5	0.0	0.0	1.3944854237227629	0.0
15	6.374790508446916E-5	0.0	0.0	1.4291642840887142	0.0
16	6.374790508446916E-5	0.0	0.0	1.4951433658511397	0.0
17	6.374790508446916E-5	0.0	0.0	1.5880559375117536	0.0
18	6.374790508446916E-5	0.0	0.0	1.7005391160332994	0.0
19	6.374790508446916E-5	0.0	0.0	1.7834751405481937	0.0
20	6.374790508446916E-5	0.0	0.0	1.8773439307850746	0.0
21	6.374790508446916E-5	0.0	0.0	1.9852691340930808	0.0
22	6.374790508446916E-5	0.0	0.0	2.116812936234883	0.0
23	6.374790508446916E-5	0.0	0.0	2.249026091380072	0.0
24	6.374790508446916E-5	0.0	0.0	2.349174050267773	0.0
25	6.374790508446916E-5	0.0	0.0	2.43599869699282	0.0
26	6.374790508446916E-5	0.0	0.0	2.5202734275144882	0.0
27	6.374790508446916E-5	0.0	0.0	2.6157996632835654	0.0
28	6.374790508446916E-5	0.0	0.0	2.7178600593238005	0.0
29	6.374790508446916E-5	0.0	0.0	2.8463439620215483	0.0
30	6.374790508446916E-5	0.0	0.0	2.9878643113090697	0.0
31	6.374790508446916E-5	0.0	0.0	3.105574818047542	0.0
32	6.374790508446916E-5	0.0	0.0	3.217834878901292	0.0
33	6.374790508446916E-5	0.0	0.0	3.337808436270263	0.0
34	6.374790508446916E-5	0.0	0.0	3.4675991710222425	0.0
35	6.374790508446916E-5	0.0	0.0	3.613358755997881	0.0
36	6.374790508446916E-5	0.0	0.0	3.7505442477396587	0.0
37	6.374790508446916E-5	0.0	0.0	3.899937463305112	0.0
38	6.374790508446916E-5	0.0	0.0	4.066606361148457	0.0
39	6.374790508446916E-5	0.0	0.0	4.327494662706647	0.0
40	6.374790508446916E-5	0.0	0.0	4.508506839193997	0.0
41	6.374790508446916E-5	0.0	0.0	4.685184158135603	0.0
42	6.374790508446916E-5	0.0	0.0	4.8591203171585775	0.0
43	6.374790508446916E-5	0.0	0.0	5.0191594328731375	0.0
44	6.374790508446916E-5	0.0	0.0	5.182640935462258	0.0
45	6.374790508446916E-5	0.0	0.0	5.347748009631034	0.0
46	6.374790508446916E-5	0.0	0.0	5.519452991976051	0.0
47	6.374790508446916E-5	0.0	0.0	5.707541185927777	0.0
48	6.374790508446916E-5	0.0	0.0	5.88817087498462	0.0
49	6.374790508446916E-5	0.0	0.0	6.069661160760105	0.0
50	6.374790508446916E-5	0.0	0.0	6.254816951077945	0.0
51	6.374790508446916E-5	0.0	0.0	6.451829851741497	0.0
52	6.374790508446916E-5	0.0	0.0	6.676349973448998	0.0
53	6.374790508446916E-5	0.0	0.0	6.87180105043798	0.0
54	6.374790508446916E-5	0.0	0.0	7.065945295372731	0.0
55	6.374790508446916E-5	0.0	0.0	7.2664643308159285	0.0
56	6.374790508446916E-5	0.0	0.0	7.472975669337067	0.0
57	6.374790508446916E-5	0.0	0.0	7.67400468802094	0.0
58	6.374790508446916E-5	0.0	0.0	7.8743962276539685	0.0
59	6.374790508446916E-5	0.0	0.0	8.087919835734398	0.0
60	6.374790508446916E-5	0.0	0.0	8.320376571624916	0.0
61	6.374790508446916E-5	0.0	0.0	8.552865181467974	0.0
62	9.562185762670374E-5	0.0	0.0	8.816717760612592	0.0
63	9.562185762670374E-5	0.0	0.0	9.079932860706364	0.0
64	9.562185762670374E-5	0.0	0.0	9.328549690535795	0.0
65	9.562185762670374E-5	0.0	0.0	9.562886989626303	0.0
66	9.562185762670374E-5	0.0	0.0	9.79862674262867	0.0
67	9.562185762670374E-5	0.0	0.0	10.030573495278512	0.0
68	9.562185762670374E-5	0.0	0.0	10.296912242721424	0.0
69	9.562185762670374E-5	0.0	0.0	10.66400455415034	0.0
70	9.562185762670374E-5	0.0	0.0	10.9344550414712	0.0
71	9.562185762670374E-5	0.0	0.0	11.1897654013345	3.187395254223458E-5
72	9.562185762670374E-5	0.0	0.0	11.437967869780879	3.187395254223458E-5
73	9.562185762670374E-5	0.0	0.0	11.689007120003518	3.187395254223458E-5
74	9.562185762670374E-5	0.0	0.0	11.972972163202288	3.187395254223458E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	18855	0.0	75.92681	1
TAACGCG	80	0.0	75.00001	1
CGAGGGA	2670	0.0	68.164795	4
CCGATCC	1675	0.0	68.0597	80
TAGGGAT	7215	0.0	65.91823	5
TACGGGA	1985	0.0	65.08816	4
GCGGGAT	2505	0.0	64.670654	5
ACGGGAT	2305	0.0	64.38178	5
AGGGATT	7215	0.0	63.75606	6
CGGGACT	1415	0.0	63.60424	6
CGGGATC	1455	0.0	63.505157	6
ACGGGAC	1425	0.0	63.157894	5
TAGGGAC	4330	0.0	62.817554	5
CGTAAGG	590	0.0	62.37288	2
ATAGGGA	5595	0.0	62.1269	4
TAAGGGA	5690	0.0	62.07381	4
GTACGGG	1265	0.0	61.97628	3
GCGTAAG	215	0.0	61.395348	1
GCGGGGT	2020	0.0	61.188118	5
CGGGATT	2010	0.0	61.094524	6
>>END_MODULE