FastQCFastQC Report
Thu 26 May 2016
SRR1547822_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547822_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2797015
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135050.48283616641312255No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84900.069.870441
CGAAACG3300.069.0909180
GTACGGG10250.068.292693
CGGGATC9000.066.222226
ACGGGAT19000.065.684215
TACGGGA16400.065.3658454
TAAGGGA48200.065.1452264
TAGGGAC37600.064.787245
CGGGATA8900.063.820236
TAGGGAT61800.063.430425
AAGGGAT59950.063.252715
GCGGGAT22450.063.2516675
AGGGATT72850.062.704196
ATAGGGA46700.062.0985074
CGAGGGA23850.062.054514
CGTTAGG7650.061.699352
GCGGGGT15900.061.6352235
CCGATCC19250.061.09090880
CGGGATT16750.060.8955236
GTAGGGA55050.060.8900954
Produced by FastQC (version 0.11.5)