##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547822_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2797015
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02299701646219	33.0	31.0	34.0	31.0	34.0
2	32.45780305075232	34.0	31.0	34.0	31.0	34.0
3	32.58698362361303	34.0	31.0	34.0	31.0	34.0
4	36.10436340169788	37.0	35.0	37.0	35.0	37.0
5	36.06318271442949	37.0	35.0	37.0	35.0	37.0
6	36.126951410700336	37.0	36.0	37.0	35.0	37.0
7	36.34578506014448	37.0	37.0	37.0	35.0	37.0
8	36.35258445163862	37.0	37.0	37.0	35.0	37.0
9	38.14881579112018	39.0	39.0	39.0	37.0	39.0
10-11	37.71493717409453	39.0	37.5	39.0	35.0	39.0
12-13	37.163275313146336	39.0	37.0	39.0	33.5	39.0
14-15	38.388884936262414	40.0	37.0	41.0	33.5	41.0
16-17	38.51572319061571	40.0	37.0	41.0	34.0	41.0
18-19	38.377810093975185	40.0	37.0	41.0	34.0	41.0
20-21	38.23190472700361	40.0	36.0	41.0	34.0	41.0
22-23	38.046600751157925	40.0	35.5	41.0	34.0	41.0
24-25	38.03811170122434	40.0	35.0	41.0	34.0	41.0
26-27	38.02434970852856	40.0	35.0	41.0	34.0	41.0
28-29	37.94394220266963	40.0	36.0	41.0	34.0	41.0
30-31	37.81468637100623	40.0	35.5	41.0	33.5	41.0
32-33	37.54900635141392	40.0	35.0	41.0	33.0	41.0
34-35	37.30858701151048	40.0	35.0	41.0	33.0	41.0
36-37	37.12815626659135	40.0	35.0	41.0	32.0	41.0
38-39	37.03541132242766	40.0	35.0	41.0	32.0	41.0
40-41	36.932423136808346	40.0	35.0	41.0	31.0	41.0
42-43	36.881924122680786	40.0	35.0	41.0	31.0	41.0
44-45	36.74297367014478	39.5	35.0	41.0	31.0	41.0
46-47	36.62619042085938	39.0	35.0	41.0	31.0	41.0
48-49	36.52585595715432	39.0	35.0	41.0	31.0	41.0
50-51	36.3107553945903	39.0	35.0	40.5	30.5	41.0
52-53	36.13176386254632	38.5	35.0	40.0	30.0	41.0
54-55	35.95032132469794	38.0	35.0	40.0	30.0	41.0
56-57	35.65600327491987	37.5	35.0	40.0	29.5	41.0
58-59	35.405179271473344	37.0	35.0	40.0	29.0	41.0
60-61	35.05234294417441	36.5	34.0	40.0	28.0	41.0
62-63	34.70588520261779	36.0	34.0	39.0	27.5	41.0
64-65	34.38262719363321	35.5	34.0	39.0	27.0	40.0
66-67	34.082016363873635	35.0	34.0	38.5	26.5	40.0
68-69	33.642647071967794	35.0	33.5	37.5	26.0	40.0
70-71	33.36801876285969	35.0	33.0	37.0	26.0	39.5
72-73	33.102598126931746	35.0	33.0	37.0	26.0	39.0
74-75	32.818032795676814	35.0	33.0	36.0	25.0	39.0
76-77	30.842654043685858	33.5	30.0	34.5	21.5	36.0
78-79	31.927727416549427	34.5	32.0	35.0	24.0	37.0
80-81	32.061656801983546	35.0	33.0	35.0	24.0	37.0
82-83	31.867004109738417	35.0	32.5	35.0	24.0	36.0
84-85	31.653611439338007	34.5	32.0	35.0	23.0	36.0
86	31.228724908518547	34.0	31.0	35.0	20.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	10.0
10	13.0
11	20.0
12	36.0
13	87.0
14	328.0
15	937.0
16	2377.0
17	5268.0
18	10616.0
19	16693.0
20	20392.0
21	20952.0
22	18823.0
23	16704.0
24	16846.0
25	18741.0
26	22013.0
27	26086.0
28	32057.0
29	39475.0
30	49841.0
31	63285.0
32	79217.0
33	106095.0
34	248582.0
35	238428.0
36	247474.0
37	468845.0
38	700947.0
39	325188.0
40	635.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.20958772119563	23.56183288255515	30.598834829273354	13.629744566975866
2	29.831159289456792	25.47948437888249	31.350207274540892	13.339149057119823
3	29.01568279040334	24.87791449098414	32.93464639982267	13.171756318789853
4	27.05519991848453	26.928135887723165	32.27254769817108	13.744116495621226
5	23.640345153672754	32.59417629151077	29.59240476007458	14.173073794741894
6	22.114968993730816	41.08923262835559	26.137221287694203	10.658577090219394
7	89.61696665909908	3.1310522110178174	5.703187147727131	1.548793982155977
8	90.56790900299069	2.5742443283285934	5.418240517122718	1.4396061515580003
9	85.9413338863038	5.584524931042558	6.396640704465296	2.0775004781883544
10-11	35.38595609962764	31.950668838029113	20.707986907471003	11.955388154872248
12-13	28.01000352161143	24.51354390305379	29.378337263118006	18.098115312216773
14-15	17.826897603337844	30.639789203847673	33.73993346478299	17.793379728031493
16-17	23.531193075475105	27.370232193963922	30.51299331608876	18.585581414472212
18-19	24.192844872122603	28.222515789153796	29.567020555842564	18.017618782881037
20-21	26.963870411849776	27.2000328922083	29.291923711528185	16.54417298441374
22-23	22.574637604732185	24.15827230100661	32.38983344744308	20.877256646818125
24-25	20.68414005645304	28.11186211014242	32.12040335858048	19.083594474824054
26-27	19.597338591319673	32.12072513018342	29.4252622885469	18.85667398995
28-29	18.876498695931197	28.289462158765684	33.84229258691855	18.991746558384566
30-31	22.564644808840853	27.14946112194607	30.792130181640072	19.493763887573003
32-33	23.654324342200525	28.182526729388297	28.9944637408094	19.168685187601785
34-35	20.978900720947152	28.052745516202094	30.375078431828218	20.593275331022536
36-37	22.16337774377327	28.486153989163448	29.638954385300043	19.71151388176324
38-39	20.616156867231673	29.925331111917526	28.289354901564707	21.169157119286098
40-41	20.906376976884285	27.64323752285919	29.460961060273185	21.989424439983342
42-43	21.518440194278543	27.415888009181216	28.462503776347287	22.603168020192957
44-45	20.85782521724052	29.200790843095227	28.88579432001616	21.05558961964809
46-47	21.220175794552407	28.616811136157654	28.69001417582673	21.47299889346321
48-49	21.047044795969992	26.987377615064634	30.847653659347557	21.117923929617824
50-51	19.758778554995235	29.157476810099336	30.206881264490892	20.87686337041453
52-53	21.826911904297972	26.880674576289366	29.66659099075264	21.62582252866002
54-55	21.561646970073454	26.81401422588009	30.43378387316478	21.19055493088167
56-57	21.723927115156695	26.78879090744955	29.869879139010695	21.61740283838306
58-59	22.70484784672231	27.319142013896958	29.18856352218347	20.78744661719726
60-61	23.23684356358475	28.697808199097967	27.532458710446672	20.53288952687061
62-63	22.71709304383423	26.39142085401759	30.024758537226294	20.866727564921888
64-65	21.147955945892317	28.923924254964668	29.672168365203618	20.255951433939394
66-67	20.6888951256965	28.7098031294076	27.324272483343847	23.277029261552048
68-69	19.638382346894815	30.538752920524203	27.223200447620048	22.599664284960934
70-71	20.3535554868315	29.257583531014316	29.01729164841805	21.371569333736144
72-73	22.168776356222615	28.830163585107695	28.24983062300345	20.75122943566624
74-75	20.729062947463635	29.876421828270495	27.31708625087817	22.0774289733877
76-77	22.889473242009785	27.917601442966877	27.765242588974314	21.427682726049017
78-79	20.088862591012205	28.659088349544064	29.175406638863215	22.076642420580512
80-81	18.97279778621137	28.148722834879326	29.645693712761638	23.232785666147663
82-83	20.254074432922238	27.87528847718014	29.984108058054748	21.886529031842876
84-85	19.03731299260104	27.456913888556194	29.048360484302016	24.457412634540752
86	19.55406031072411	27.86660064390073	30.088433562208284	22.490905483166877
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	938.0
1	1125.5
2	4503.5
3	5095.5
4	2789.0
5	3338.5
6	3903.0
7	4210.0
8	4344.0
9	4618.5
10	4781.0
11	4728.0
12	4789.0
13	4987.5
14	5050.0
15	5126.5
16	5191.5
17	5447.0
18	5780.5
19	5920.0
20	6278.5
21	6564.0
22	7640.5
23	10107.5
24	12726.0
25	16348.5
26	21443.0
27	25085.0
28	29524.5
29	33022.0
30	36991.5
31	44725.5
32	53129.5
33	58730.5
34	65154.5
35	73587.0
36	76557.0
37	80668.0
38	89440.5
39	98167.5
40	107117.5
41	118595.5
42	128942.5
43	132696.0
44	136584.5
45	141301.0
46	147314.5
47	154153.0
48	148524.5
49	133593.0
50	125943.0
51	117722.5
52	105066.0
53	94395.0
54	83931.0
55	76672.5
56	68311.5
57	62980.0
58	58786.0
59	46199.0
60	32728.0
61	24736.5
62	19555.5
63	14592.0
64	11896.0
65	9907.0
66	6753.0
67	4940.5
68	3561.5
69	2321.0
70	1527.0
71	1242.0
72	933.0
73	582.5
74	358.5
75	204.0
76	160.0
77	78.0
78	49.0
79	30.0
80	31.5
81	25.0
82	19.0
83	18.0
84	11.5
85	9.0
86	10.0
87	8.0
88	4.0
89	2.0
90	2.0
91	1.0
92	1.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2797015.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.802733982585714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.83657180485983	13.414726781186626
2	7.692542461776103	2.5851148926993774
3	2.349073301169288	1.18412561355426
4	1.1387526699794501	0.7653663274249569
5	0.717979125661908	0.603200612677326
6	0.48103454150789604	0.4849617262435358
7	0.3920038496620068	0.4610715484214155
8	0.30685602620430974	0.41248161434114944
9	0.2547944994607935	0.38531197751992124
>10	3.4808493802294995	14.68397373829952
>50	1.672211036074224	20.48800348304956
>100	1.6726070317427035	43.40856938873483
>500	0.0034358339432937397	0.3845993954070166
>1k	0.0010736981072792938	0.2512037705998358
>5k	0.0	0.0
>10k+	2.1473962145585873E-4	0.4872891298406754
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13505	0.48283616641312255	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.5752400326776936E-5	0.0	0.0	0.01973532498038087	0.0
2	3.5752400326776936E-5	0.0	0.0	0.06835858942479751	0.0
3	3.5752400326776936E-5	0.0	0.0	0.10300266534144437	0.0
4	3.5752400326776936E-5	0.0	0.0	0.1722550647744113	0.0
5	3.5752400326776936E-5	0.0	0.0	0.3337129046501359	0.0
6	3.5752400326776936E-5	0.0	0.0	0.47489913354057806	0.0
7	3.5752400326776936E-5	0.0	0.0	0.5716451288248364	0.0
8	3.5752400326776936E-5	0.0	0.0	0.7656019005976014	0.0
9	3.5752400326776936E-5	0.0	0.0	0.8293841827805715	0.0
10	3.5752400326776936E-5	0.0	0.0	0.9541600599210229	0.0
11	3.5752400326776936E-5	0.0	0.0	1.1510485285205836	0.0
12	7.150480065355387E-5	0.0	0.0	1.325055460911007	0.0
13	7.150480065355387E-5	0.0	0.0	1.3839039118488818	0.0
14	7.150480065355387E-5	0.0	0.0	1.4062134096527905	0.0
15	7.150480065355387E-5	0.0	0.0	1.4413580191740123	0.0
16	7.150480065355387E-5	0.0	0.0	1.5199060426919413	0.0
17	7.150480065355387E-5	0.0	0.0	1.6290223684892644	0.0
18	7.150480065355387E-5	0.0	0.0	1.7593756200806931	0.0
19	7.150480065355387E-5	0.0	0.0	1.8507230029156083	0.0
20	7.150480065355387E-5	0.0	0.0	1.954011687459667	0.0
21	7.150480065355387E-5	0.0	0.0	2.0806466894171107	0.0
22	7.150480065355387E-5	0.0	0.0	2.2345250204235585	0.0
23	7.150480065355387E-5	0.0	0.0	2.3788574605427573	0.0
24	7.150480065355387E-5	0.0	0.0	2.4844342987077295	0.0
25	7.150480065355387E-5	0.0	0.0	2.581144541591661	0.0
26	7.150480065355387E-5	0.0	0.0	2.6724561720262496	0.0
27	7.150480065355387E-5	0.0	0.0	2.775923618571942	0.0
28	7.150480065355387E-5	0.0	0.0	2.8862555259803755	0.0
29	7.150480065355387E-5	0.0	0.0	3.0229369524296437	0.0
30	7.150480065355387E-5	0.0	0.0	3.178888922655045	0.0
31	1.0725720098033081E-4	0.0	0.0	3.3130676810814386	0.0
32	1.0725720098033081E-4	0.0	0.0	3.4419550842594693	0.0
33	1.4300960130710774E-4	0.0	0.0	3.5708067350371735	0.0
34	1.4300960130710774E-4	0.0	0.0	3.713208545538726	0.0
35	1.4300960130710774E-4	0.0	0.0	3.865942799734717	0.0
36	1.4300960130710774E-4	0.0	0.0	4.005877694613722	0.0
37	1.7876200163388469E-4	0.0	0.0	4.158325929607099	0.0
38	1.7876200163388469E-4	0.0	0.0	4.3373381980432715	0.0
39	1.7876200163388469E-4	0.0	0.0	4.604730400087236	0.0
40	1.7876200163388469E-4	0.0	0.0	4.777092722062627	0.0
41	1.7876200163388469E-4	0.0	0.0	4.948275214827236	0.0
42	1.7876200163388469E-4	0.0	0.0	5.117741592376158	0.0
43	1.7876200163388469E-4	0.0	0.0	5.280629528264954	0.0
44	2.502668022874386E-4	0.0	0.0	5.449130591005054	0.0
45	2.502668022874386E-4	0.0	0.0	5.611339231287641	0.0
46	2.502668022874386E-4	0.0	0.0	5.79936110460616	0.0
47	2.860192026142155E-4	0.0	0.0	5.993067609576638	0.0
48	2.860192026142155E-4	0.0	0.0	6.176370166052023	0.0
49	2.860192026142155E-4	0.0	0.0	6.362068133349303	0.0
50	2.860192026142155E-4	0.0	0.0	6.5571689819325245	0.0
51	3.2177160294099243E-4	0.0	0.0	6.750732477301695	0.0
52	3.2177160294099243E-4	0.0	0.0	6.977438447773787	0.0
53	3.2177160294099243E-4	0.0	0.0	7.176936841597203	0.0
54	3.2177160294099243E-4	0.0	0.0	7.38151207626702	0.0
55	3.5752400326776937E-4	0.0	0.0	7.587124130546314	0.0
56	3.5752400326776937E-4	0.0	0.0	7.801424018105015	0.0
57	3.5752400326776937E-4	0.0	0.0	8.007679615590192	0.0
58	3.5752400326776937E-4	0.0	0.0	8.213935213075368	0.0
59	3.5752400326776937E-4	0.0	0.0	8.424087822196162	0.0
60	3.5752400326776937E-4	0.0	0.0	8.652938936687862	0.0
61	3.5752400326776937E-4	0.0	0.0	8.891621961269426	0.0
62	3.5752400326776937E-4	0.0	0.0	9.15247147405359	0.0
63	3.5752400326776937E-4	0.0	0.0	9.411783633623703	0.0
64	3.5752400326776937E-4	0.0	0.0	9.66394531312846	0.0
65	3.5752400326776937E-4	0.0	0.0	9.902735594911004	0.0
66	3.5752400326776937E-4	0.0	0.0	10.135090444634727	0.0
67	3.5752400326776937E-4	0.0	0.0	10.37631188963949	0.0
68	3.5752400326776937E-4	0.0	0.0	10.638269726833785	0.0
69	3.5752400326776937E-4	0.0	0.0	10.992254242469203	0.0
70	3.5752400326776937E-4	0.0	0.0	11.244344417173307	0.0
71	3.5752400326776937E-4	0.0	0.0	11.504729148753224	0.0
72	3.5752400326776937E-4	0.0	0.0	11.7537088646289	0.0
73	3.932764035945463E-4	0.0	0.0	12.011304908983327	0.0
74	3.932764035945463E-4	0.0	0.0	12.276873738610625	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	8490	0.0	69.87044	1
CGAAACG	330	0.0	69.09091	80
GTACGGG	1025	0.0	68.29269	3
CGGGATC	900	0.0	66.22222	6
ACGGGAT	1900	0.0	65.68421	5
TACGGGA	1640	0.0	65.365845	4
TAAGGGA	4820	0.0	65.145226	4
TAGGGAC	3760	0.0	64.78724	5
CGGGATA	890	0.0	63.82023	6
TAGGGAT	6180	0.0	63.43042	5
AAGGGAT	5995	0.0	63.25271	5
GCGGGAT	2245	0.0	63.251667	5
AGGGATT	7285	0.0	62.70419	6
ATAGGGA	4670	0.0	62.098507	4
CGAGGGA	2385	0.0	62.05451	4
CGTTAGG	765	0.0	61.69935	2
GCGGGGT	1590	0.0	61.635223	5
CCGATCC	1925	0.0	61.090908	80
CGGGATT	1675	0.0	60.895523	6
GTAGGGA	5505	0.0	60.890095	4
>>END_MODULE