FastQCFastQC Report
Thu 26 May 2016
SRR1547821_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547821_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3602151
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323080.896908541590844No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT179250.073.1269151
TATTGCG3650.067.9452061
CGAGGGA28750.064.6956564
CGAACGG1800.064.444442
TACGGGA20700.064.3478244
TAGGGAC42300.063.924355
GCGGGAT28200.062.6950345
TGCGGGA33000.062.5454564
GCGAGGG28500.062.315793
TAGGGAT73250.062.3071675
TAACGGG12650.062.2924923
AGGGATC50400.062.222226
ATAGGGA63450.061.717894
AGGGATT88250.061.3711056
AAGGGAT74450.061.3566175
GTAGGGA62550.061.326944
ACGGGAC16100.060.8695645
GCGGGAC19550.060.5626565
TAAGGGA60850.060.542324
CCGATCC20900.060.4784780
Produced by FastQC (version 0.11.5)