##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547821_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3602151
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19628993898368	33.0	31.0	34.0	31.0	34.0
2	32.647983663094635	34.0	31.0	34.0	31.0	34.0
3	32.75780582213239	34.0	31.0	34.0	31.0	34.0
4	36.27672771074838	37.0	37.0	37.0	35.0	37.0
5	36.25614362085321	37.0	37.0	37.0	35.0	37.0
6	36.31043340492944	37.0	37.0	37.0	35.0	37.0
7	36.4456317905607	37.0	37.0	37.0	35.0	37.0
8	36.44397361465413	37.0	37.0	37.0	35.0	37.0
9	38.246936622035	39.0	39.0	39.0	37.0	39.0
10-11	37.93192845052859	39.0	38.0	39.0	35.0	39.0
12-13	37.51646252475257	39.0	37.0	39.0	35.0	39.0
14-15	38.82110772146976	40.0	38.0	41.0	35.0	41.0
16-17	38.92862500766903	40.5	38.0	41.0	35.0	41.0
18-19	38.73344648794567	40.0	37.5	41.0	35.0	41.0
20-21	38.540205699316886	40.0	37.0	41.0	35.0	41.0
22-23	38.37034011067276	40.0	36.0	41.0	34.5	41.0
24-25	38.3502329580298	40.0	36.0	41.0	35.0	41.0
26-27	38.35916650912191	40.0	36.0	41.0	35.0	41.0
28-29	38.254863829972706	40.0	36.0	41.0	34.5	41.0
30-31	38.06717930480982	40.0	36.0	41.0	34.0	41.0
32-33	37.698108713377096	40.0	36.0	41.0	33.5	41.0
34-35	37.32802122953757	40.0	35.5	41.0	33.0	41.0
36-37	37.142055677288376	40.0	35.0	41.0	32.5	41.0
38-39	37.03857736669006	40.0	35.0	41.0	32.0	41.0
40-41	36.91894023876289	40.0	35.0	41.0	32.0	41.0
42-43	36.86716964391554	40.0	35.0	41.0	32.0	41.0
44-45	36.72731320813591	40.0	35.0	41.0	31.0	41.0
46-47	36.61038834851732	40.0	35.0	41.0	31.0	41.0
48-49	36.49825382111966	39.0	35.0	41.0	31.0	41.0
50-51	36.284956960438365	39.0	35.0	41.0	30.5	41.0
52-53	36.08658881873636	39.0	35.0	40.5	30.0	41.0
54-55	35.89757453255014	38.0	35.0	40.0	30.0	41.0
56-57	35.629851580347406	38.0	35.0	40.0	29.0	41.0
58-59	35.399678691981535	37.5	35.0	40.0	29.0	41.0
60-61	35.063544532142046	37.0	35.0	40.0	28.0	41.0
62-63	34.72185716256759	36.0	34.0	39.5	27.0	41.0
64-65	34.390911569226276	36.0	34.0	39.0	26.5	40.5
66-67	34.07274348021502	35.5	34.0	39.0	26.0	40.0
68-69	33.607663032449224	35.0	34.0	38.0	25.5	40.0
70-71	33.31770850250309	35.0	34.0	37.0	25.0	39.5
72-73	33.02322501194425	35.0	33.5	37.0	25.0	39.0
74-75	32.70533453483766	35.0	33.0	36.5	24.0	39.0
76-77	30.77541252435003	33.5	30.0	34.5	20.5	36.5
78-79	31.85496776786981	34.5	32.5	35.0	22.5	37.0
80-81	31.94351569381739	35.0	33.0	35.0	23.0	37.0
82-83	31.75237601088905	35.0	33.0	35.0	20.5	36.5
84-85	31.522995426899094	35.0	32.5	35.0	20.0	36.0
86	31.09054756449688	34.0	32.0	35.0	19.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	11.0
10	21.0
11	16.0
12	67.0
13	103.0
14	345.0
15	1008.0
16	2857.0
17	7470.0
18	18124.0
19	30938.0
20	37154.0
21	36711.0
22	30521.0
23	24291.0
24	20483.0
25	19746.0
26	21923.0
27	25611.0
28	31629.0
29	39814.0
30	51784.0
31	65675.0
32	85128.0
33	116622.0
34	287968.0
35	288409.0
36	318486.0
37	614239.0
38	965752.0
39	458266.0
40	975.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.69184190224119	22.888046614370136	29.555451728703215	15.864659754685464
2	32.113062445189	24.733249661105265	30.596496371195986	12.557191522509745
3	28.33895636246232	25.27706362115303	33.640871801320934	12.743108215063723
4	26.352143483157704	27.020355337685732	32.69041192332026	13.937089255836305
5	24.134274215600623	30.702405312825586	30.98121094868039	14.182109522893406
6	21.994885833492265	39.743170122518464	27.36961887494444	10.89232516904483
7	87.04140942453552	3.209526752209999	7.954164053644614	1.7948997696098805
8	87.68791202811876	2.871090079233214	7.827156607260495	1.6138412853875364
9	83.18371439731426	5.566951524242043	8.774007530500526	2.4753265479431596
10-11	32.9533659194187	30.7579970967347	23.554329066160747	12.73430791768585
12-13	26.789923576218765	23.67518740885654	31.377987763422468	18.156901251502227
14-15	18.09631245330915	29.612070676659584	34.28366828597691	18.00794858405436
16-17	23.8057205264299	27.748573005407046	30.87737021574054	17.56833625242251
18-19	23.600801299001624	28.33316815425006	30.59109681965026	17.47493372709806
20-21	26.09365903872436	27.43371946373153	30.92925588072238	15.543365616821728
22-23	22.533175316637198	23.576801749843355	33.863655354814384	20.026367578705056
24-25	20.32688246550464	27.952562232954698	33.14311365625705	18.57744164528361
26-27	19.374090647504783	31.193348085629946	30.945065878693036	18.487495388172235
28-29	18.50703926626063	28.160035489905894	34.412952149979276	18.9199730938542
30-31	21.584006333993216	27.574982281420183	32.089257224364	18.7517541602226
32-33	22.95035660637214	27.69861674316263	30.44818221112885	18.902844439336384
34-35	20.758749424996342	27.885852092263764	31.530854758726107	19.82454372401379
36-37	22.091244925601398	28.94298434463186	30.046283456745705	18.919487273021037
38-39	20.9327149250545	29.094088504340874	29.82713661920336	20.14605995140126
40-41	20.84501732437091	27.716231218513602	30.294648947254014	21.14410250986147
42-43	21.008530736218443	27.795517178485856	29.8833669104932	21.3125851748025
44-45	20.822447476521667	29.269816839993663	29.500165317889227	20.407570365595447
46-47	20.871445977694993	28.816254510152405	30.113298970531776	20.199000541620826
48-49	21.190907876987943	27.75547166123797	31.01051288521775	20.04310757655634
50-51	20.101392195940704	29.0958929817212	30.255200295601153	20.54751452673694
52-53	21.341887111339865	26.958822659016796	30.872040067170975	20.827250162472367
54-55	21.109650872492576	27.249593367962643	30.81733664135679	20.823419118187996
56-57	21.152541911763276	27.49300348597269	30.4028065453114	20.95164805695264
58-59	22.31407012088055	27.9522290986691	29.455289353500174	20.278411426950175
60-61	22.368301606456807	28.856119579662266	28.967303147480493	19.808275666400437
62-63	21.595624392203437	27.65854346472427	30.419407737210353	20.326424405861943
64-65	21.19097727996411	29.44975099600211	29.74837812184997	19.610893602183808
66-67	20.526874081625117	29.64227485188711	27.949147606527326	21.881703459960452
68-69	19.809205666281063	30.310597751177003	28.51761350370931	21.36258307883262
70-71	20.428155288326337	29.51490651002693	29.402543091613868	20.654395110032866
72-73	21.830775556049705	29.469364277066674	28.736121833870932	19.963738333012692
74-75	20.635947798967894	30.26055820536119	27.90438268690013	21.19911130877079
76-77	22.31058609147701	28.96945463974164	28.34616039138837	20.37379887739298
78-79	19.720328215002645	29.450000846716307	29.33816489092212	21.491506047358925
80-81	19.214324996370223	29.17209744955167	29.71406529043341	21.899512263644695
82-83	20.282186948853614	28.88177091965328	29.59857873809288	21.237463393400223
84-85	19.376103333813603	28.774487799095596	29.22792520357975	22.621483663511054
86	19.33647423442271	28.663956619253327	30.044687188293885	21.954881958030075
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1393.0
1	1655.5
2	9457.0
3	11018.5
4	5566.0
5	6736.0
6	7874.0
7	8367.0
8	8615.0
9	8939.0
10	9257.5
11	9611.5
12	9848.0
13	10012.5
14	10052.0
15	10212.0
16	10268.0
17	10487.5
18	10965.5
19	11519.0
20	12667.0
21	13416.0
22	14841.0
23	16335.0
24	18657.5
25	22199.5
26	27575.0
27	34391.0
28	43744.0
29	50368.0
30	55221.5
31	63894.5
32	74361.0
33	85882.5
34	92872.5
35	100243.5
36	105499.0
37	110402.5
38	121746.5
39	132141.0
40	141654.0
41	150636.5
42	158707.5
43	163355.0
44	163634.5
45	165932.5
46	172713.5
47	176750.5
48	176068.0
49	166010.5
50	155910.0
51	143807.0
52	126332.0
53	116088.0
54	105070.0
55	93229.0
56	80949.5
57	74365.0
58	67980.0
59	54272.5
60	41070.5
61	32929.0
62	26814.5
63	19175.5
64	15389.0
65	13379.5
66	9167.5
67	5953.5
68	4456.0
69	3264.0
70	2078.5
71	1599.0
72	1437.5
73	1103.0
74	663.0
75	275.5
76	131.5
77	143.0
78	137.0
79	96.0
80	73.0
81	47.5
82	32.5
83	19.0
84	14.5
85	9.5
86	8.0
87	8.0
88	7.5
89	5.5
90	2.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	3602151.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.498672888494838
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.9954535998751	13.198188215111061
2	7.28483132585172	2.403800981861754
3	2.267481297303354	1.1223129661496385
4	1.197179918100149	0.790075194296376
5	0.7113880292174389	0.5868479195424767
6	0.5610137644477496	0.555358955133991
7	0.41289906627156775	0.47686006412656845
8	0.3063484691325432	0.4043474545687192
9	0.2614847858428348	0.38827367522451517
>10	3.6126567871484627	14.766728253178425
>50	1.6612983163207449	20.049397454359884
>100	1.7208081590991926	43.40393091004085
>500	0.005452557249044599	0.5855268796120635
>1k	0.0015335317262937939	0.36009284621427506
>5k	0.0	0.0
>10k+	1.7039241403264373E-4	0.9082582305793426
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32308	0.896908541590844	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7761190466474058E-5	0.0	0.0	0.023069549277639944	0.0
2	2.7761190466474058E-5	0.0	0.0	0.08517133235114241	0.0
3	2.7761190466474058E-5	0.0	0.0	0.13283729638207836	0.0
4	2.7761190466474058E-5	0.0	0.0	0.19274594540872939	0.0
5	2.7761190466474058E-5	0.0	0.0	0.3505127908297015	0.0
6	2.7761190466474058E-5	0.0	0.0	0.4934829217320429	0.0
7	2.7761190466474058E-5	0.0	0.0	0.5846229100334772	0.0
8	2.7761190466474058E-5	0.0	0.0	0.728203787126081	0.0
9	2.7761190466474058E-5	0.0	0.0	0.7974679573399338	0.0
10	2.7761190466474058E-5	0.0	0.0	0.9304440596743446	0.0
11	2.7761190466474058E-5	0.0	0.0	1.126354780796252	0.0
12	2.776119046647406E-4	0.0	0.0	1.2833720740746293	0.0
13	2.776119046647406E-4	0.0	0.0	1.3452517676244	0.0
14	2.776119046647406E-4	0.0	0.0	1.3807860914214867	0.0
15	2.776119046647406E-4	0.0	0.0	1.4284242942619563	0.0
16	2.776119046647406E-4	0.0	0.0	1.5117911492327778	0.0
17	2.776119046647406E-4	0.0	0.0	1.6205039710994902	0.0
18	2.776119046647406E-4	0.0	0.0	1.7426254479615098	0.0
19	2.776119046647406E-4	0.0	0.0	1.8301009591213695	0.0
20	2.776119046647406E-4	0.0	0.0	1.9257382602783726	0.0
21	2.776119046647406E-4	0.0	0.0	2.0379489921438605	0.0
22	2.776119046647406E-4	0.0	0.0	2.1616806180529355	0.0
23	2.776119046647406E-4	0.0	0.0	2.285440005152477	0.0
24	2.776119046647406E-4	0.0	0.0	2.379494918452891	0.0
25	2.776119046647406E-4	0.0	0.0	2.464749534375433	0.0
26	2.776119046647406E-4	0.0	0.0	2.5492823593458462	0.0
27	2.776119046647406E-4	0.0	0.0	2.638978765743024	0.0
28	2.776119046647406E-4	0.0	0.0	2.7324229328531757	0.0
29	2.776119046647406E-4	0.0	0.0	2.845466500432658	0.0
30	2.776119046647406E-4	0.0	0.0	2.9655336492001583	0.0
31	3.0537309513121465E-4	0.0	0.0	3.071081695353693	0.0
32	3.0537309513121465E-4	0.0	0.0	3.1710219810329994	0.0
33	3.0537309513121465E-4	0.0	0.0	3.2717395800453675	0.0
34	3.0537309513121465E-4	0.0	0.0	3.3825900135779983	0.0
35	3.0537309513121465E-4	0.0	0.0	3.5024350728217666	0.0
36	3.0537309513121465E-4	0.0	0.0	3.61342431230673	0.0
37	3.0537309513121465E-4	0.0	0.0	3.7303822077419855	0.0
38	3.0537309513121465E-4	0.0	0.0	3.8641356234094575	0.0
39	3.0537309513121465E-4	0.0	0.0	4.063821866434806	0.0
40	3.0537309513121465E-4	0.0	0.0	4.208513191146068	0.0
41	3.0537309513121465E-4	0.0	0.0	4.348151979192433	0.0
42	3.0537309513121465E-4	0.0	0.0	4.489289871523987	0.0
43	3.0537309513121465E-4	0.0	0.0	4.6168803029079015	0.0
44	3.0537309513121465E-4	0.0	0.0	4.740500884055111	0.0
45	3.0537309513121465E-4	0.0	0.0	4.865870420201707	0.0
46	3.0537309513121465E-4	0.0	0.0	5.008146521342387	0.0
47	3.0537309513121465E-4	0.0	0.0	5.150644712006798	0.0
48	3.0537309513121465E-4	0.0	0.0	5.286230366245057	0.0
49	3.0537309513121465E-4	0.0	0.0	5.428534228576203	0.0
50	3.0537309513121465E-4	0.0	0.0	5.573031224954201	0.0
51	3.0537309513121465E-4	0.0	0.0	5.723358071330158	0.0
52	3.0537309513121465E-4	0.0	0.0	5.900030287458799	0.0
53	3.0537309513121465E-4	0.0	0.0	6.051995044072278	0.0
54	3.0537309513121465E-4	0.0	0.0	6.214120396396487	0.0
55	3.0537309513121465E-4	0.0	0.0	6.370277092770403	0.0
56	3.0537309513121465E-4	0.0	0.0	6.528210505334174	0.0
57	3.3313428559768874E-4	0.0	0.0	6.687643022183135	0.0
58	3.608954760641628E-4	0.0	0.0	6.845743001889704	0.0
59	3.608954760641628E-4	0.0	0.0	7.014725368259132	0.0
60	3.886566665306368E-4	0.0	0.0	7.186789226770338	0.0
61	4.164178569971109E-4	0.0	0.0	7.366876069326355	0.0
62	4.164178569971109E-4	0.0	0.0	7.586411563535232	0.0
63	4.164178569971109E-4	0.0	0.0	7.784293329180259	0.0
64	4.164178569971109E-4	0.0	0.0	7.978149722207648	0.0
65	4.164178569971109E-4	0.0	0.0	8.153017460955967	0.0
66	4.164178569971109E-4	0.0	0.0	8.330161617322538	0.0
67	4.164178569971109E-4	0.0	0.0	8.524101293921326	0.0
68	4.4417904746358493E-4	0.0	0.0	8.730255894325364	0.0
69	4.4417904746358493E-4	0.0	0.0	9.001732575897012	0.0
70	4.4417904746358493E-4	0.0	0.0	9.20158538606516	0.0
71	4.4417904746358493E-4	0.0	0.0	9.390722376713247	0.0
72	4.4417904746358493E-4	0.0	0.0	9.590103246643464	0.0
73	4.4417904746358493E-4	0.0	0.0	9.780767102767207	0.0
74	4.71940237930059E-4	0.0	0.0	10.003606178641595	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	17925	0.0	73.126915	1
TATTGCG	365	0.0	67.945206	1
CGAGGGA	2875	0.0	64.695656	4
CGAACGG	180	0.0	64.44444	2
TACGGGA	2070	0.0	64.347824	4
TAGGGAC	4230	0.0	63.92435	5
GCGGGAT	2820	0.0	62.695034	5
TGCGGGA	3300	0.0	62.545456	4
GCGAGGG	2850	0.0	62.31579	3
TAGGGAT	7325	0.0	62.307167	5
TAACGGG	1265	0.0	62.292492	3
AGGGATC	5040	0.0	62.22222	6
ATAGGGA	6345	0.0	61.71789	4
AGGGATT	8825	0.0	61.371105	6
AAGGGAT	7445	0.0	61.356617	5
GTAGGGA	6255	0.0	61.32694	4
ACGGGAC	1610	0.0	60.869564	5
GCGGGAC	1955	0.0	60.562656	5
TAAGGGA	6085	0.0	60.54232	4
CCGATCC	2090	0.0	60.47847	80
>>END_MODULE