FastQCFastQC Report
Thu 26 May 2016
SRR1547820_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547820_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1310864
Sequences flagged as poor quality0
Sequence length86
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259201.9773218274359505No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86800.076.267281
CTAGCGG1550.067.096782
ACGGGAT7150.065.4545445
TTACGGG5550.064.864873
TACGGGA5900.064.406784
TAGCGGG8200.064.390243
TAGACGG2500.064.02
GTACGCG251.235591E-464.01
CGAACGG700.062.8571432
TATGGGA13300.062.8571434
TTAGGGA17700.062.8248564
CGGGATT8150.062.3312846
CGGGACT4350.061.6091966
CGGGTAC650.061.5384646
TAGGGAT20050.061.446395
ATAGGGA19100.061.3612564
TTATGGG16900.061.301773
GCGGGAT9500.061.052635
TATCGGG1050.060.9523853
AGGGTAA12650.060.711466
Produced by FastQC (version 0.11.5)