##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547820_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1310864
Sequences flagged as poor quality	0
Sequence length	86
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09794685032162	33.0	31.0	34.0	31.0	34.0
2	32.53006948089199	34.0	31.0	34.0	31.0	34.0
3	32.59770121080448	34.0	31.0	34.0	31.0	34.0
4	36.14958836309488	37.0	35.0	37.0	35.0	37.0
5	36.11913211439173	37.0	35.0	37.0	35.0	37.0
6	36.17906739371895	37.0	36.0	37.0	35.0	37.0
7	36.37791258284612	37.0	37.0	37.0	35.0	37.0
8	36.37581472982705	37.0	37.0	37.0	35.0	37.0
9	38.167630661914586	39.0	39.0	39.0	37.0	39.0
10-11	37.78664605939289	39.0	37.5	39.0	35.0	39.0
12-13	37.22565803927791	39.0	37.0	39.0	34.5	39.0
14-15	38.49158799082132	40.0	37.0	41.0	34.0	41.0
16-17	38.60177333422842	40.0	37.0	41.0	35.0	41.0
18-19	38.38196029488948	40.0	37.0	41.0	35.0	41.0
20-21	38.11406065007506	40.0	35.0	41.0	34.0	41.0
22-23	37.90590442639359	40.0	35.0	41.0	34.0	41.0
24-25	37.8426137265193	40.0	35.0	41.0	34.0	41.0
26-27	37.79483417044026	40.0	35.0	41.0	34.0	41.0
28-29	37.689265629386426	40.0	35.0	41.0	34.0	41.0
30-31	37.49123669579758	40.0	35.0	41.0	33.5	41.0
32-33	37.07353089260213	40.0	35.0	41.0	33.0	41.0
34-35	36.67774116918308	40.0	35.0	41.0	31.5	41.0
36-37	36.45945803683677	39.0	35.0	41.0	31.0	41.0
38-39	36.359845109790186	39.0	35.0	41.0	31.0	41.0
40-41	36.222392635696764	39.0	35.0	41.0	30.0	41.0
42-43	36.161294001513504	39.0	35.0	41.0	30.0	41.0
44-45	36.02370879053815	39.0	35.0	41.0	30.0	41.0
46-47	35.8886112518156	38.5	35.0	41.0	29.0	41.0
48-49	35.76986971951324	38.0	35.0	40.5	29.0	41.0
50-51	35.54720398149617	38.0	35.0	40.0	28.5	41.0
52-53	35.34927803341918	38.0	35.0	40.0	27.5	41.0
54-55	35.16873527688608	37.0	35.0	40.0	27.5	41.0
56-57	34.8847759950689	37.0	34.5	40.0	26.5	41.0
58-59	34.63118942926192	36.0	34.0	40.0	26.0	41.0
60-61	34.26418453783154	36.0	34.0	39.0	25.5	41.0
62-63	33.847537196841174	35.0	33.5	39.0	24.5	40.5
64-65	33.44896915316921	35.0	33.0	38.5	23.0	40.0
66-67	33.12466243637784	35.0	33.0	37.5	22.5	40.0
68-69	32.655365850309416	35.0	33.0	37.0	19.5	39.5
70-71	32.3333045991041	35.0	33.0	36.5	19.5	39.0
72-73	32.050104740079824	35.0	33.0	36.0	19.0	39.0
74-75	31.752676097596698	35.0	32.5	36.0	18.0	38.0
76-77	29.837661649110814	33.0	29.5	34.5	14.0	36.0
78-79	30.852672359604046	34.0	31.0	35.0	15.0	36.5
80-81	30.987036031197743	35.0	32.0	35.0	15.0	36.0
82-83	30.798951302347156	34.5	31.5	35.0	12.0	36.0
84-85	30.578271277569606	34.0	31.0	35.0	10.5	36.0
86	30.151610693405267	34.0	31.0	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	14.0
12	21.0
13	43.0
14	200.0
15	509.0
16	1359.0
17	3589.0
18	7972.0
19	13090.0
20	15671.0
21	14787.0
22	12479.0
23	10118.0
24	9413.0
25	10021.0
26	11168.0
27	13676.0
28	16856.0
29	20803.0
30	26371.0
31	32863.0
32	39605.0
33	50398.0
34	121320.0
35	134833.0
36	114089.0
37	200940.0
38	299354.0
39	129002.0
40	296.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.20008177812496	23.52784117955791	29.08829596357822	17.183781078738907
2	32.51977321827436	25.67596638552893	29.43821784716035	12.36604254903636
3	27.35585079764186	25.020978530190774	35.55891381562084	12.064256856546523
4	26.079974734221096	25.76529678135947	34.48229564623027	13.672432838189163
5	23.651500079336987	30.277435340355673	32.62771729180144	13.443347288505903
6	20.938556555065972	39.58343504741911	29.051755178264106	10.426253219250816
7	85.26109497247617	3.5179850846464618	9.645623039460997	1.5752969034163726
8	85.8658106409208	3.3721270856473287	9.31957853751419	1.442483735917685
9	81.72968362850762	5.396288249581955	10.401612981972196	2.472415139938239
10-11	35.789639504937206	28.373576511369603	22.53738755507818	13.29939642861502
12-13	26.708758498211864	26.658562596882668	29.724364998962514	16.90831390594295
14-15	16.30714551623967	32.01037636246018	34.53706868141928	17.145409439880872
16-17	20.44479823993946	27.913917843498638	33.03279363839422	18.608490278167682
18-19	22.577971475301787	30.545006957243466	29.737676829938113	17.13934473751663
20-21	24.857460423049226	29.047330615532964	30.064255330835238	16.03095363058258
22-23	21.030061089479915	26.549817524930123	32.400882166265916	20.01923921932405
24-25	18.780704939642863	29.57721014536977	33.92682230956072	17.71526260542665
26-27	18.223095607172063	34.23429127659315	30.177386822736764	17.36522629349803
28-29	16.837826044501945	29.867820002685253	34.96068242014427	18.33367153266853
30-31	21.404813924251485	29.719749722320543	31.226351475057673	17.649084878370296
32-33	23.247453587862662	29.679165802096936	29.256314919015246	17.817065691025157
34-35	20.01683622404765	29.433449999389715	30.611833111596624	19.937880664966006
36-37	21.6209690707808	30.59482143075102	30.25489295609613	17.529316542372054
38-39	19.980486152644364	31.777667248471236	28.778157001794234	19.463689597090163
40-41	20.53626463157124	28.713810128281803	29.624316481343605	21.125608758803356
42-43	20.75981947784057	29.76349949346385	28.20792240842681	21.26875862026877
44-45	19.524069621257432	32.06061040657154	28.345045710310146	20.07027426186088
46-47	21.137280450145855	30.856023203017248	28.616279034285785	19.39041731255111
48-49	20.765922322986977	29.214281573069368	30.413566929902718	19.606229174040937
50-51	19.35917837395794	30.962746707515045	29.81503039216883	19.86304452635819
52-53	20.938747268976794	28.766790452709053	29.909967776977624	20.384494501336523
54-55	21.025789097877432	29.004076700557803	30.239406986537126	19.730727215027645
56-57	21.470724651832683	28.033381037239565	29.8125511113281	20.683343199599655
58-59	21.706752187869984	29.704683323365355	28.91180931050056	19.67675517826411
60-61	22.421929353464584	31.52298789195523	27.200609674230126	18.854473080350058
62-63	22.044468381159295	29.40045649281695	29.908213208998035	18.646861917025717
64-65	20.295469247763307	31.860437085769384	29.006594124180694	18.837499542286615
66-67	19.016846904026657	31.710459666296426	26.854883496686156	22.41780993299076
68-69	18.372272028219555	34.02793882508025	26.238648708027686	21.36114043867251
70-71	19.046254989075905	32.16538862917893	28.55235173137717	20.236004650368
72-73	21.556927339525686	31.990160687912706	27.05868038179399	19.394231590767617
74-75	20.228032808895506	33.16434809408146	25.854703462754337	20.752915634268696
76-77	22.97000299039412	30.955080008299866	26.972630265229654	19.10228673607636
78-79	19.149736357089676	30.93204176787218	28.79711396453026	21.121107910507877
80-81	17.874470578183548	30.62750979506646	29.39885449596602	22.09916513078397
82-83	19.5686203908262	29.900966080386677	29.78955101368258	20.740862515104542
84-85	18.07052447851188	30.088781139767363	28.79814381964872	23.042550562072037
86	19.133182389630047	30.173076688352108	29.111181632877248	21.582559289140598
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	720.0
1	999.5
2	9215.5
3	10075.0
4	3391.0
5	5090.0
6	7417.5
7	8439.0
8	8787.0
9	8387.0
10	7082.0
11	6039.5
12	5340.5
13	4947.0
14	4767.0
15	4876.0
16	5166.5
17	5082.5
18	4904.5
19	5000.0
20	5041.0
21	5074.0
22	5174.0
23	5682.0
24	6673.5
25	7914.0
26	8845.5
27	10757.5
28	14289.0
29	16183.0
30	18478.5
31	20961.5
32	22701.5
33	24211.0
34	27313.0
35	31541.0
36	32624.0
37	35311.0
38	39380.5
39	41579.5
40	46938.5
41	53455.5
42	58898.5
43	62367.0
44	63197.5
45	65940.0
46	66879.0
47	67062.5
48	69434.0
49	65555.0
50	60461.0
51	53938.0
52	45480.0
53	42152.5
54	36652.0
55	31022.0
56	27041.5
57	24583.0
58	22673.0
59	18351.0
60	13443.0
61	9661.0
62	7283.5
63	5416.5
64	4641.0
65	3821.5
66	2472.0
67	1733.5
68	1286.5
69	946.5
70	745.0
71	645.0
72	511.5
73	286.0
74	199.5
75	132.0
76	59.5
77	66.5
78	49.5
79	26.0
80	25.5
81	17.5
82	5.5
83	3.0
84	5.0
85	3.0
86	1.0
87	2.0
88	2.0
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1310864.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.676771794940898
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.4478607758462	16.01371743268526
2	7.786903821047976	3.220160665939246
3	2.919796136294957	1.811158751937629
4	1.5540124440983798	1.285278426924822
5	0.9831985644509459	1.0164686173132849
6	0.7044242114282575	0.8739131199919968
7	0.5233262804946206	0.7574488653257749
8	0.41555002827675624	0.687378648324778
9	0.34338224075478235	0.639003260746988
>10	4.690200755611254	23.726014468395977
>50	1.9242941171867862	28.62441348910715
>100	0.7018407778021917	18.562487088807483
>500	0.0037213190763636804	0.505681654870764
>1k	0.0011163957229091042	0.2824646005828628
>5k	0.0	0.0
>10k+	3.721319076363681E-4	1.9944109090459832
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25920	1.9773218274359505	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03577792967081253	0.0
2	0.0	0.0	0.0	0.14639199794944402	0.0
3	0.0	0.0	0.0	0.23495953813667933	0.0
4	0.0	0.0	0.0	0.35068473922542687	0.0
5	0.0	0.0	0.0	0.6950377766114563	0.0
6	0.0	0.0	0.0	1.0179545704207302	0.0
7	0.0	0.0	0.0	1.2397167059283039	0.0
8	0.0	0.0	0.0	1.5397478304385503	0.0
9	0.0	0.0	0.0	1.6932343858706929	0.0
10	0.0	0.0	0.0	1.9495538820197977	0.0
11	0.0	0.0	0.0	2.325260286345494	0.0
12	0.0	0.0	0.0	2.5947008995593746	0.0
13	0.0	0.0	0.0	2.689294999328687	0.0
14	0.0	0.0	0.0	2.7323963431751883	0.0
15	0.0	0.0	0.0	2.7940350791539017	0.0
16	0.0	0.0	0.0	2.9187619768335997	0.0
17	0.0	0.0	0.0	3.0697311086428494	0.0
18	7.628556433009069E-5	0.0	0.0	3.229473080350059	0.0
19	7.628556433009069E-5	0.0	0.0	3.3448168536171563	0.0
20	7.628556433009069E-5	0.0	0.0	3.44871779223474	0.0
21	7.628556433009069E-5	0.0	0.0	3.5821412442480685	0.0
22	7.628556433009069E-5	0.0	0.0	3.7252529629313185	0.0
23	7.628556433009069E-5	0.0	0.0	3.8731706721673644	0.0
24	7.628556433009069E-5	0.0	0.0	3.9901164422853936	0.0
25	7.628556433009069E-5	0.0	0.0	4.0879908213209	0.0
26	7.628556433009069E-5	0.0	0.0	4.187467197207338	0.0
27	7.628556433009069E-5	0.0	0.0	4.287325000915427	0.0
28	7.628556433009069E-5	0.0	0.0	4.390157941632389	0.0
29	7.628556433009069E-5	0.0	0.0	4.51717340624199	0.0
30	7.628556433009069E-5	0.0	0.0	4.658759413638639	0.0
31	7.628556433009069E-5	0.0	0.0	4.780358603180803	0.0
32	7.628556433009069E-5	0.0	0.0	4.89600751870522	0.0
33	7.628556433009069E-5	0.0	0.0	5.015775854703462	0.0
34	7.628556433009069E-5	0.0	0.0	5.146453026400908	0.0
35	7.628556433009069E-5	0.0	0.0	5.29139559862808	0.0
36	7.628556433009069E-5	0.0	0.0	5.422377912582846	0.0
37	7.628556433009069E-5	0.0	0.0	5.554199367745243	0.0
38	7.628556433009069E-5	0.0	0.0	5.697158515299833	0.0
39	7.628556433009069E-5	0.0	0.0	5.880396440820711	0.0
40	7.628556433009069E-5	0.0	0.0	6.036247848747086	0.0
41	7.628556433009069E-5	0.0	0.0	6.19110754433717	0.0
42	7.628556433009069E-5	0.0	0.0	6.351841228380671	0.0
43	7.628556433009069E-5	0.0	0.0	6.504336071476522	0.0
44	7.628556433009069E-5	0.0	0.0	6.650499212732976	0.0
45	7.628556433009069E-5	0.0	0.0	6.802536342442846	0.0
46	7.628556433009069E-5	0.0	0.0	6.959684604962834	0.0
47	7.628556433009069E-5	0.0	0.0	7.1142391582955975	0.0
48	7.628556433009069E-5	0.0	0.0	7.271387420815584	0.0
49	7.628556433009069E-5	0.0	0.0	7.431510820344444	0.0
50	7.628556433009069E-5	0.0	0.0	7.604907908066741	0.0
51	7.628556433009069E-5	0.0	0.0	7.777694711274396	0.0
52	7.628556433009069E-5	0.0	0.0	7.967569480891992	0.0
53	7.628556433009069E-5	0.0	0.0	8.146916842632034	0.0
54	7.628556433009069E-5	0.0	0.0	8.320237644790001	0.0
55	7.628556433009069E-5	0.0	0.0	8.511104126743888	0.0
56	7.628556433009069E-5	0.0	0.0	8.69815633048127	0.0
57	7.628556433009069E-5	0.0	0.0	8.881546827130808	0.0
58	7.628556433009069E-5	0.0	0.0	9.063335326929414	0.0
59	7.628556433009069E-5	0.0	0.0	9.247336108093593	0.0
60	7.628556433009069E-5	0.0	0.0	9.448424855667712	0.0
61	7.628556433009069E-5	0.0	0.0	9.661185294584335	0.0
62	7.628556433009069E-5	0.0	0.0	9.896449974978335	0.0
63	7.628556433009069E-5	0.0	0.0	10.124620097889636	0.0
64	2.2885669299027207E-4	0.0	0.0	10.349662512663404	0.0
65	2.2885669299027207E-4	0.0	0.0	10.559066386749503	0.0
66	2.2885669299027207E-4	0.0	0.0	10.766944549549	0.0
67	2.2885669299027207E-4	0.0	0.0	10.984968692404399	0.0
68	2.2885669299027207E-4	0.0	0.0	11.214435669909312	0.0
69	2.2885669299027207E-4	0.0	0.0	11.470068595979445	0.0
70	2.2885669299027207E-4	0.0	0.0	11.687940167706184	0.0
71	2.2885669299027207E-4	0.0	0.0	11.897038899534964	0.0
72	3.0514225732036277E-4	0.0	0.0	12.115063042390362	0.0
73	3.0514225732036277E-4	0.0	0.0	12.34018174272846	0.0
74	3.0514225732036277E-4	0.0	0.0	12.586660401078984	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	8680	0.0	76.26728	1
CTAGCGG	155	0.0	67.09678	2
ACGGGAT	715	0.0	65.454544	5
TTACGGG	555	0.0	64.86487	3
TACGGGA	590	0.0	64.40678	4
TAGCGGG	820	0.0	64.39024	3
TAGACGG	250	0.0	64.0	2
GTACGCG	25	1.235591E-4	64.0	1
CGAACGG	70	0.0	62.857143	2
TATGGGA	1330	0.0	62.857143	4
TTAGGGA	1770	0.0	62.824856	4
CGGGATT	815	0.0	62.331284	6
CGGGACT	435	0.0	61.609196	6
CGGGTAC	65	0.0	61.538464	6
TAGGGAT	2005	0.0	61.44639	5
ATAGGGA	1910	0.0	61.361256	4
TTATGGG	1690	0.0	61.30177	3
GCGGGAT	950	0.0	61.05263	5
TATCGGG	105	0.0	60.952385	3
AGGGTAA	1265	0.0	60.71146	6
>>END_MODULE