FastQCFastQC Report
Thu 26 May 2016
SRR1547819_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547819_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences852556
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57540.6749116773560915No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10030.11764623086342714No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA9680.1135409286897283No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG550.080.01
AATCCGG204.0902938E-580.02
TGTTACG150.001309674680.01
ACGTAAG204.0902938E-580.01
TATCGTG500.080.01
TACGTTG404.1836756E-1180.02
CGGGATC2200.078.1818166
TACGCGG1300.076.923072
CGCTAGG700.074.285712
ACCTCCG650.073.8461580
TGATGCG600.073.333331
GCATAGG3100.072.2580642
CGCATGG451.1823431E-1071.1111152
GCGATAT451.1823431E-1071.1111159
TTACGTT451.1823431E-1071.1111151
GTACGGG3850.070.649353
CGTTTTT38950.070.038511
TCGATAG403.7125574E-970.01
ACGGGGC1600.070.05
TATAGCG403.7125574E-970.01
Produced by FastQC (version 0.11.5)