##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547819_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	852556
Sequences flagged as poor quality	0
Sequence length	86
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27713252853771	33.0	31.0	34.0	31.0	34.0
2	32.71961959097115	34.0	31.0	34.0	31.0	34.0
3	32.81260468520543	34.0	31.0	34.0	31.0	34.0
4	36.307393297331785	37.0	37.0	37.0	35.0	37.0
5	36.29545038683676	37.0	37.0	37.0	35.0	37.0
6	36.34791849450359	37.0	37.0	37.0	35.0	37.0
7	36.48665073027461	37.0	37.0	37.0	35.0	37.0
8	36.50140635923036	37.0	37.0	37.0	35.0	37.0
9	38.37034986558068	39.0	39.0	39.0	37.0	39.0
10-11	37.96032870568033	39.0	38.5	39.0	35.0	39.0
12-13	36.598855676342666	39.0	35.0	39.0	34.0	39.0
14-15	37.55833693036	39.0	35.0	41.0	33.0	41.0
16-17	37.834407358578204	39.5	35.0	41.0	35.0	41.0
18-19	37.75658490468661	38.5	35.0	41.0	35.0	41.0
20-21	37.47127696010585	38.0	35.0	41.0	34.0	41.0
22-23	37.30480167871671	38.0	35.0	41.0	34.0	41.0
24-25	37.28846081665017	38.0	35.0	41.0	34.0	41.0
26-27	37.27262373380752	38.0	35.0	41.0	34.0	41.0
28-29	37.309960870605565	38.0	35.0	41.0	34.0	41.0
30-31	37.164995613191394	38.0	35.0	41.0	34.0	41.0
32-33	36.70476367534802	38.0	35.0	41.0	33.5	41.0
34-35	36.371820150230604	38.0	35.0	41.0	33.0	41.0
36-37	36.14930749417047	37.0	35.0	41.0	32.5	41.0
38-39	36.11656770933522	37.0	35.0	41.0	32.0	41.0
40-41	36.04229458240866	37.0	35.0	41.0	31.5	41.0
42-43	35.976927028840336	37.0	35.0	41.0	32.0	41.0
44-45	35.81265043000108	36.0	35.0	40.0	32.0	41.0
46-47	35.692637785670385	36.0	35.0	40.0	31.0	41.0
48-49	35.66169788260244	36.0	35.0	40.0	31.0	41.0
50-51	35.43027730729712	35.5	35.0	40.0	31.0	41.0
52-53	35.26180860846678	35.5	35.0	40.0	30.5	41.0
54-55	35.111287117796365	35.5	35.0	40.0	31.0	41.0
56-57	34.85364890986634	35.0	34.5	39.5	30.0	41.0
58-59	34.64200064277303	35.0	35.0	39.0	29.0	41.0
60-61	34.284537320715586	35.0	34.0	38.5	28.5	40.0
62-63	33.7480095149175	35.0	33.5	37.5	26.5	40.0
64-65	33.47072509019935	35.0	33.5	37.0	26.5	40.0
66-67	33.27019163550547	35.0	34.0	36.5	26.0	39.5
68-69	33.00054014047171	35.0	34.0	36.0	25.5	39.0
70-71	32.76868381666424	35.0	33.5	35.5	25.0	39.0
72-73	32.54634475623889	35.0	33.5	35.0	25.0	37.5
74-75	32.310271114155555	35.0	33.0	35.0	24.5	37.0
76-77	30.377831485556374	33.0	30.0	34.5	20.0	35.5
78-79	31.22459697662089	34.0	31.5	35.0	20.0	36.0
80-81	31.680895448510128	35.0	33.0	35.0	20.0	36.0
82-83	31.57295239257011	35.0	33.0	35.0	20.0	36.0
84-85	31.438553596479295	35.0	33.0	35.0	19.5	35.0
86	31.053265709232004	34.0	32.0	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	2.0
12	12.0
13	15.0
14	49.0
15	206.0
16	576.0
17	1398.0
18	3572.0
19	6907.0
20	8620.0
21	8597.0
22	6591.0
23	4739.0
24	4197.0
25	4421.0
26	4951.0
27	6158.0
28	8003.0
29	10624.0
30	14506.0
31	19033.0
32	25633.0
33	38114.0
34	151872.0
35	131863.0
36	59918.0
37	115339.0
38	155473.0
39	61100.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.638404984540607	24.67919995871239	29.639812516714443	16.04258254003256
2	28.433088266342622	26.631681672523566	32.35670149526835	12.578528565865469
3	27.82116365376585	25.704704441702365	34.108609874307376	12.365522030224408
4	25.02287239782489	27.567455979431266	33.6628913525915	13.746780270152342
5	23.634576497027762	30.659921459704698	31.596047649655855	14.109454393611681
6	21.2069353801979	39.9172605670478	28.2226622063536	10.653141846400706
7	88.09403722453423	3.2680551189599276	7.183105860494794	1.4548017960110537
8	90.35875649224215	2.1695935516259346	6.16065103054814	1.310998925583774
9	87.57970150934366	3.338666316347548	7.08927038223882	1.9923617920699637
10-11	52.889370317023165	20.91862587325642	16.755321644560592	9.436682165159825
12-13	33.029208638494126	32.38045360070189	21.577233636265536	13.013104124538447
14-15	12.475778717175178	35.48195074575746	38.76408118645578	13.278189350611571
16-17	15.588594766795378	20.472496821323176	38.51236751603413	25.42654089584731
18-19	25.32320457541792	24.467718249592988	31.23935553793534	18.969721637053755
20-21	27.30594823096665	26.513331675573216	28.416021938734815	17.76469815472532
22-23	19.76667808331652	25.64922421518352	28.2678791774382	26.316218524061764
24-25	16.466425665880013	24.541144511328287	40.296649135071476	18.69578068772022
26-27	14.378293038815047	38.769594020803325	29.29361824912381	17.558494691257817
28-29	12.471966650871028	26.881225397510544	43.139688184705754	17.50711976691267
30-31	23.52578598942474	30.063714289735806	30.100310126255636	16.310189594583814
32-33	29.594478251282023	28.581876146552247	26.207779899502203	15.61586570266352
34-35	18.989661676183147	28.428337845255914	29.406279470204886	23.17572100835605
36-37	24.850039176312173	28.885023388492954	29.944543232350718	16.320394202844156
38-39	18.643408761418605	32.6836594898165	28.26307010917758	20.409861639587312
40-41	20.76631916261219	25.669164254312914	27.946727253107127	25.617789329967767
42-43	22.260004034925565	25.011788081955906	25.942049554516068	26.78615832860246
44-45	16.992197579983014	36.493555848530775	26.19464293254637	20.319603638939846
46-47	21.866246909293935	31.859666696381232	25.95981964821079	20.314266746114036
48-49	21.961959097115027	24.32491238112218	32.79127705394133	20.92185146782147
50-51	18.185667569051184	34.690624428190056	26.957584017941343	20.166123984817418
52-53	24.85496553892061	24.742069729143893	30.079549026691506	20.323415705243995
54-55	24.705239303928423	27.503002735304193	29.535479194328584	18.2562787664388
56-57	24.868219800224267	21.650777192348656	28.97328738522748	24.507715622199598
58-59	26.890550298162232	25.92773964408203	26.5831218125261	20.59858824522964
60-61	28.498773101121806	29.75904222127344	24.03865552526755	17.703529152337207
62-63	25.93055470842971	23.74981819376088	31.0640591351184	19.255567962691014
64-65	17.76452221320359	31.962181956375886	31.746184414865414	18.527111415555105
66-67	16.711981383041113	28.78831419871539	23.251082626830378	31.248621791413118
68-69	16.47498815327087	34.485652555374664	21.724203454083955	27.315155837270517
70-71	19.3030721735581	30.27191175711625	28.290868869610915	22.13414719971474
72-73	25.24960237216089	31.111446051637664	24.266147912864376	19.37280366333707
74-75	25.277166543898584	33.40126630977907	21.080785309117527	20.24078183720483
76-77	33.178289754573306	26.612621341002818	23.159006563791703	17.05008234063217
78-79	18.823807468365715	29.87463580105002	31.55581568835361	19.74574104223066
80-81	14.759558316403847	28.597534941986215	33.02879810827676	23.614108633333178
82-83	18.857822829233506	25.155825541078826	33.73004236671843	22.256309262969236
84-85	15.234893660944266	25.792675202567338	29.584273643021692	29.388157493466704
86	18.16232599383501	24.75673152262139	33.74499739606548	23.335945087478123
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	743.0
1	672.0
2	1874.0
3	2051.5
4	975.0
5	1172.5
6	1443.5
7	1536.0
8	1608.5
9	1674.5
10	1631.0
11	1641.5
12	1765.5
13	1943.5
14	2045.0
15	2082.0
16	2106.5
17	2172.5
18	2362.0
19	2522.0
20	2673.0
21	2775.0
22	2900.0
23	2780.5
24	2781.5
25	3494.0
26	4085.0
27	5264.0
28	7550.5
29	8782.0
30	10079.5
31	10880.5
32	10221.5
33	11500.5
34	12667.5
35	13493.0
36	14593.0
37	15823.0
38	17825.5
39	22587.0
40	30138.5
41	36851.0
42	45330.5
43	50660.0
44	54230.5
45	58054.5
46	63686.0
47	69767.5
48	65855.5
49	51808.0
50	42376.0
51	36077.0
52	26096.0
53	20133.0
54	16960.0
55	17906.5
56	19313.5
57	18882.0
58	16246.5
59	11331.0
60	7409.5
61	5053.0
62	4171.5
63	3131.5
64	2258.0
65	2102.0
66	1500.0
67	898.0
68	706.0
69	481.5
70	198.5
71	104.0
72	131.5
73	114.5
74	55.0
75	29.0
76	19.5
77	16.5
78	16.0
79	20.0
80	12.0
81	7.0
82	10.5
83	11.5
84	10.5
85	6.0
86	3.0
87	2.5
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	852556.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.970780449529084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.79329240502517	13.711252272002875
2	8.253817407842465	2.8014744619799146
3	2.466193834445527	1.2555970233107192
4	1.1188065004432504	0.7594807793811346
5	0.7228644257634577	0.6133786732203277
6	0.46117800461918423	0.46959303987264506
7	0.34311802273180647	0.4076086442440612
8	0.28670468902499757	0.38924818650349957
9	0.20613747750027797	0.3148482485769262
>10	2.3422220669772007	9.585514334383184
>50	1.1332835870395748	14.13939619901601
>100	1.8668296687914612	54.31349417704217
>500	0.004163932346746754	0.44330623963463334
>1k	6.939887244577924E-4	0.11812862868053592
>5k	6.939887244577924E-4	0.6776790921513496
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5754	0.6749116773560915	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1003	0.11764623086342714	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	968	0.1135409286897283	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05278245651898526	0.0
2	0.0	0.0	0.0	0.19259731912038622	0.0
3	0.0	0.0	0.0	0.2683694678120851	0.0
4	0.0	0.0	0.0	0.4396192156292373	0.0
5	0.0	0.0	0.0	0.7423559273525727	0.0
6	0.0	0.0	0.0	0.9853898160355449	0.0
7	0.0	0.0	0.0	1.1218031425501669	0.0
8	0.0	0.0	0.0	1.3703498655806774	0.0
9	0.0	0.0	0.0	1.4514002599242748	0.0
10	0.0	0.0	0.0	1.6751978755647723	0.0
11	0.0	0.0	0.0	1.9617479672889522	0.0
12	0.0	0.0	0.0	2.203374323798085	0.0
13	0.0	0.0	0.0	2.289820258141401	0.0
14	0.0	0.0	0.0	2.3238356190091913	0.0
15	0.0	0.0	0.0	2.3842422081364743	0.0
16	0.0	0.0	0.0	2.520890123346736	0.0
17	0.0	0.0	0.0	2.696245173337587	0.0
18	0.0	0.0	0.0	2.939396356368379	0.0
19	0.0	0.0	0.0	3.065839663318304	0.0
20	0.0	0.0	0.0	3.183720482877371	0.0
21	0.0	0.0	0.0	3.3567296459118228	0.0
22	0.0	0.0	0.0	3.5271583332942353	0.0
23	0.0	0.0	0.0	3.7310159098053384	0.0
24	0.0	0.0	0.0	3.863910405885361	0.0
25	0.0	0.0	0.0	3.978389689357649	0.0
26	0.0	0.0	0.0	4.093690033264677	0.0
27	0.0	0.0	0.0	4.199020357607008	0.0
28	0.0	0.0	0.0	4.318191414992095	0.0
29	0.0	0.0	0.0	4.44181965759434	0.0
30	0.0	0.0	0.0	4.593950426716837	0.0
31	0.0	0.0	0.0	4.749365437578294	0.0
32	0.0	0.0	0.0	4.880735107136657	0.0
33	0.0	0.0	0.0	5.000961813652124	0.0
34	0.0	0.0	0.0	5.132214188862667	0.0
35	0.0	0.0	0.0	5.312378307114137	0.0
36	0.0	0.0	0.0	5.456063883193597	0.0
37	0.0	0.0	0.0	5.616991728402592	0.0
38	0.0	0.0	0.0	5.764430723612291	0.0
39	0.0	0.0	0.0	5.913511839691469	0.0
40	0.0	0.0	0.0	6.081125462726202	0.0
41	0.0	0.0	0.0	6.23055846184884	0.0
42	0.0	0.0	0.0	6.380343344014938	0.0
43	0.0	0.0	0.0	6.5199236179207	0.0
44	0.0	0.0	0.0	6.676042394869076	0.0
45	0.0	0.0	0.0	6.839550715730111	0.0
46	0.0	0.0	0.0	7.0233509587639995	0.0
47	0.0	0.0	0.0	7.195656355711531	0.0
48	1.1729434781996725E-4	0.0	0.0	7.371128700050202	0.0
49	1.1729434781996725E-4	0.0	0.0	7.563139547431488	0.0
50	1.1729434781996725E-4	0.0	0.0	7.768521950464251	0.0
51	1.1729434781996725E-4	0.0	0.0	7.947278536541881	0.0
52	1.1729434781996725E-4	0.0	0.0	8.140579621749188	0.0
53	1.1729434781996725E-4	0.0	0.0	8.332355880434834	0.0
54	1.1729434781996725E-4	0.0	0.0	8.533515686946078	0.0
55	1.1729434781996725E-4	0.0	0.0	8.7386635012832	0.0
56	1.1729434781996725E-4	0.0	0.0	8.936304477359846	0.0
57	1.1729434781996725E-4	0.0	0.0	9.138285344305828	0.0
58	1.1729434781996725E-4	0.0	0.0	9.31047344690554	0.0
59	1.1729434781996725E-4	0.0	0.0	9.506003124721426	0.0
60	1.1729434781996725E-4	0.0	0.0	9.70739751992831	0.0
61	1.1729434781996725E-4	0.0	0.0	9.92157699904757	0.0
62	1.1729434781996725E-4	0.0	0.0	10.154758162513664	0.0
63	1.1729434781996725E-4	0.0	0.0	10.483065042061753	0.0
64	1.1729434781996725E-4	0.0	0.0	10.728444817701124	0.0
65	1.1729434781996725E-4	0.0	0.0	10.942155119429104	0.0
66	1.1729434781996725E-4	0.0	0.0	11.150352586809547	0.0
67	1.1729434781996725E-4	0.0	0.0	11.360192175059469	0.0
68	1.1729434781996725E-4	0.0	0.0	11.588564270264945	0.0
69	1.1729434781996725E-4	0.0	0.0	11.831715453295736	0.0
70	1.1729434781996725E-4	0.0	0.0	12.049179174153956	0.0
71	1.1729434781996725E-4	0.0	0.0	12.266173717620894	0.0
72	1.1729434781996725E-4	0.0	0.0	12.485514148044235	0.0
73	1.1729434781996725E-4	0.0	0.0	12.71529377542355	0.0
74	1.1729434781996725E-4	0.0	0.0	12.96947062714942	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	55	0.0	80.0	1
AATCCGG	20	4.0902938E-5	80.0	2
TGTTACG	15	0.0013096746	80.0	1
ACGTAAG	20	4.0902938E-5	80.0	1
TATCGTG	50	0.0	80.0	1
TACGTTG	40	4.1836756E-11	80.0	2
CGGGATC	220	0.0	78.181816	6
TACGCGG	130	0.0	76.92307	2
CGCTAGG	70	0.0	74.28571	2
ACCTCCG	65	0.0	73.84615	80
TGATGCG	60	0.0	73.33333	1
GCATAGG	310	0.0	72.258064	2
CGCATGG	45	1.1823431E-10	71.111115	2
GCGATAT	45	1.1823431E-10	71.111115	9
TTACGTT	45	1.1823431E-10	71.111115	1
GTACGGG	385	0.0	70.64935	3
CGTTTTT	3895	0.0	70.03851	1
TCGATAG	40	3.7125574E-9	70.0	1
ACGGGGC	160	0.0	70.0	5
TATAGCG	40	3.7125574E-9	70.0	1
>>END_MODULE