FastQCFastQC Report
Thu 26 May 2016
SRR1547818_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547818_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1815645
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96800.5331438689831989No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG550.072.727271
TATAGCG1000.072.01
CGTTTTT58300.071.286451
TCGATTG800.070.01
AGGGTCG1500.066.6666646
CGATAGG3550.065.352112
CGTTTGG3850.064.415592
CGGGTAC1750.064.000016
GGCCGTA251.2357187E-464.09
GGGCGAT17850.063.641467
CGTTAGG3800.063.1578942
AGGGATT29650.063.136596
ACGAGGG6700.062.6865653
TACGTAG1150.062.6086961
GGTACGA459.427822E-962.2222258
TAGGGTA11500.061.9130445
CGAGGGA12050.061.742744
GTAGCGG3400.061.1764682
TAAGGGA23550.060.9766464
TAGGGAC18200.060.879125
Produced by FastQC (version 0.11.5)