##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547818_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1815645
Sequences flagged as poor quality	0
Sequence length	86
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06627892567104	33.0	31.0	34.0	31.0	34.0
2	32.49011618460657	34.0	31.0	34.0	31.0	34.0
3	32.61344480886957	34.0	31.0	34.0	31.0	34.0
4	36.12323444285639	37.0	35.0	37.0	35.0	37.0
5	36.09144573966827	37.0	35.0	37.0	35.0	37.0
6	36.13892418396768	37.0	36.0	37.0	35.0	37.0
7	36.346224069132454	37.0	37.0	37.0	35.0	37.0
8	36.3523001467798	37.0	37.0	37.0	35.0	37.0
9	38.16824819829868	39.0	39.0	39.0	37.0	39.0
10-11	37.72963795235302	39.0	37.5	39.0	35.0	39.0
12-13	36.768475941056764	39.0	35.0	39.0	33.5	39.0
14-15	37.828175937476765	40.0	35.0	41.0	33.0	41.0
16-17	38.05689245419671	40.0	35.0	41.0	34.0	41.0
18-19	37.94822611248344	40.0	36.0	41.0	34.0	41.0
20-21	37.758820419189874	40.0	35.0	41.0	34.0	41.0
22-23	37.5658190340072	39.5	35.0	41.0	33.5	41.0
24-25	37.53189307381123	39.0	35.0	41.0	34.0	41.0
26-27	37.52312621685407	39.0	35.0	41.0	34.0	41.0
28-29	37.49266541642227	39.0	35.0	41.0	34.0	41.0
30-31	37.35665562375905	39.0	35.0	41.0	33.5	41.0
32-33	37.0442592026525	39.0	35.0	41.0	33.0	41.0
34-35	36.77137766468665	39.0	35.0	41.0	32.0	41.0
36-37	36.58079250073665	39.0	35.0	41.0	31.5	41.0
38-39	36.50667834295801	39.0	35.0	41.0	31.0	41.0
40-41	36.3861831470359	39.0	35.0	41.0	31.0	41.0
42-43	36.3349107342019	38.5	35.0	41.0	31.0	41.0
44-45	36.196877693602	38.0	35.0	41.0	31.0	41.0
46-47	36.079644974650876	38.0	35.0	41.0	31.0	41.0
48-49	36.00801175339893	38.0	35.0	40.0	31.0	41.0
50-51	35.77247947699027	37.5	35.0	40.0	30.0	41.0
52-53	35.57784836793536	37.0	35.0	40.0	30.0	41.0
54-55	35.40073389897255	37.0	35.0	40.0	29.5	41.0
56-57	35.10689451957845	36.0	34.5	40.0	28.5	41.0
58-59	34.87283444726255	36.0	34.0	40.0	28.0	41.0
60-61	34.55740549501692	35.0	34.0	39.0	27.5	41.0
62-63	34.14113717163873	35.0	33.5	39.0	26.5	40.0
64-65	33.862339003494625	35.0	33.5	38.5	26.0	40.0
66-67	33.591859366781506	35.0	34.0	37.5	26.0	40.0
68-69	33.231316694618165	35.0	33.5	37.0	25.5	39.5
70-71	32.986969644396346	35.0	33.0	36.5	25.0	39.0
72-73	32.750275246537726	35.0	33.0	36.0	25.0	39.0
74-75	32.4741830038361	35.0	33.0	35.5	24.5	38.0
76-77	30.5231708290993	33.0	30.0	34.5	20.5	36.0
78-79	31.531803849320767	34.0	31.5	35.0	22.5	36.5
80-81	31.8190059180071	35.0	32.5	35.0	23.0	36.0
82-83	31.679499296393292	35.0	32.5	35.0	22.0	36.0
84-85	31.504986382249832	35.0	32.0	35.0	20.0	36.0
86	31.088454516163676	34.0	31.0	35.0	20.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	8.0
10	3.0
11	9.0
12	16.0
13	68.0
14	196.0
15	589.0
16	1573.0
17	3585.0
18	7191.0
19	11562.0
20	14453.0
21	15363.0
22	13380.0
23	11758.0
24	11817.0
25	13343.0
26	15657.0
27	18524.0
28	22433.0
29	27863.0
30	34847.0
31	43778.0
32	55845.0
33	77848.0
34	227857.0
35	207829.0
36	145900.0
37	272771.0
38	384200.0
39	175043.0
40	334.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.706583610782946	23.6854671480383	30.676536437464375	13.931412803714382
2	30.21758108000187	25.662946225721438	30.749623412065684	13.369849282211005
3	29.008368926745042	24.79229144463813	33.036303903020695	13.163035725596137
4	26.5121210368767	26.860977779246493	32.08264831506159	14.544252868815214
5	24.263058031718757	31.64456708222147	29.46908674327856	14.623288142781215
6	21.850912485645598	40.82648315061589	27.210825904843734	10.111778458894772
7	88.43584511289376	3.621247545638052	6.2872422747838925	1.6556650666842914
8	89.61685792101429	2.6272757064293955	6.034825089706413	1.7210412828498962
9	85.50267260395066	4.932462017630098	7.37440413737267	2.1904612410465703
10-11	43.50151048250071	26.655155605859076	18.62718207579125	11.216151835848969
12-13	30.940712529156304	27.563455411162423	25.966364570166522	15.529467489514746
14-15	15.64066764152684	32.99615288230904	35.627366583225246	15.735812892938872
16-17	20.548620462700583	24.096147650008675	33.79498745624833	21.56024443104241
18-19	24.660217168003655	26.88661605104522	29.629442980318288	18.823723800632834
20-21	27.22159342823074	26.806864778081618	28.155751812716694	17.81578998097095
22-23	22.286184799341278	24.60874785544531	29.739514056988014	23.365553288225396
24-25	19.24858659044031	26.01430896458283	35.32455408408582	19.412550360891036
26-27	17.129257095963144	34.342974535220264	29.52958865857588	18.998179710240713
28-29	16.099843306373216	28.082389453885533	36.85332209765676	18.964445142084493
30-31	22.506079106873866	28.114086178740887	30.10448077680384	19.27535393758141
32-33	26.28671904474718	28.654307422431145	27.301812854385084	17.75716067843659
34-35	19.93305409372427	28.650644812174185	29.655852327960584	21.760448766140957
36-37	22.940965882647767	29.083741590454082	29.255003042995742	18.720289483902413
38-39	19.867099570675986	31.044972998576263	28.038520746070954	21.049406684676793
40-41	20.46303104406423	25.49837660996505	29.25481027403485	24.78378207193587
42-43	21.528079552996317	25.811350787185823	27.89518325443575	24.76538640538211
44-45	19.337783542487657	32.36227346204792	27.27474258459115	21.02520041087327
46-47	21.509766501711514	29.823864246590055	27.479766143712013	21.186603107986418
48-49	21.071464961487514	26.533408237843854	31.699754081882748	20.695372718785887
50-51	18.664909715280245	31.402724651570104	28.60567456744022	21.32669106570943
52-53	22.46394531970732	25.91497787287713	29.82039440529399	21.80068240212156
54-55	22.706063134588533	26.74594978643953	29.899594909797894	20.64839216917404
56-57	23.370014512748913	24.2708789438464	28.7027199700382	23.65638657336649
58-59	23.640662133842245	26.893610810483327	27.986968818243657	21.478758237430775
60-61	25.365283411680146	28.886896942959666	25.973799944372384	19.774019700987804
62-63	23.73275062030298	25.49341969382781	30.254868104723116	20.518961581146094
64-65	20.002808919144435	30.195054650000415	29.96177666889728	19.84035976195787
66-67	18.83790608846994	28.476216440989287	25.448036372749076	27.237841097791694
68-69	17.941282574512087	32.452489335745696	24.33438254724905	25.271845542493164
70-71	19.48277333950194	29.98570755847096	28.214160807867177	22.317358294159927
72-73	23.308163214725344	29.404068526611756	26.194245020364665	21.093523238298236
74-75	22.188065398246902	31.788372727047413	23.96005276361844	22.063509111087246
76-77	26.926519225950006	27.74790776831374	25.160012006752417	20.165560998983832
78-79	18.680276155305688	29.60240024894734	29.885688006190637	21.831635589556328
80-81	16.70488999776939	28.668324479730344	30.48415301449347	24.1426325080068
82-83	18.78494970107042	27.336621421037705	31.258533468822375	22.619895409069503
84-85	16.18438626493615	27.013017412544855	29.381431942918358	27.421164379600636
86	19.068375150428636	26.477477700761987	30.866992170826347	23.58715497798303
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	753.0
1	924.0
2	3168.5
3	3503.5
4	1839.5
5	2118.5
6	2477.5
7	2632.0
8	2752.0
9	2812.0
10	2859.5
11	3021.0
12	3071.0
13	3277.5
14	3488.0
15	3529.5
16	3706.0
17	3844.5
18	3884.0
19	4002.0
20	4269.0
21	4454.0
22	5017.0
23	6063.5
24	8098.0
25	9810.0
26	11298.5
27	13583.5
28	18996.0
29	23451.0
30	24226.0
31	27037.5
32	30026.5
33	32801.0
34	36662.5
35	38858.5
36	39015.0
37	41891.5
38	47002.0
39	54103.5
40	65843.0
41	76470.0
42	88631.0
43	97037.0
44	97486.0
45	102168.5
46	109649.0
47	117174.5
48	114115.5
49	94116.5
50	81455.0
51	73313.5
52	62794.0
53	56750.5
54	52048.0
55	48326.5
56	44519.0
57	43396.0
58	38665.0
59	29806.0
60	21632.0
61	15697.0
62	12220.0
63	9123.5
64	7615.0
65	6709.5
66	4952.5
67	3851.0
68	3040.0
69	2137.0
70	1288.5
71	782.0
72	680.0
73	547.0
74	395.5
75	205.5
76	98.0
77	67.5
78	78.0
79	81.0
80	69.5
81	36.0
82	10.0
83	6.5
84	4.0
85	1.0
86	1.0
87	2.5
88	2.5
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1815645.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.705246143538862
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.47978520395299	12.639548362072187
2	8.66658583091052	2.7222172739711197
3	2.597104617751377	1.2236450184692038
4	1.1796763132511048	0.7410842747724422
5	0.6947907666466054	0.5455930004221504
6	0.4640465318190376	0.4372779002564115
7	0.31251677149198187	0.3435706974185965
8	0.2355301613629878	0.29592473267465197
9	0.19913239559379345	0.2814680959157772
>10	2.1941443399059524	8.035506238555506
>50	1.0210234945671786	11.721206415844192
>100	1.9415023593305936	59.07069213454357
>500	0.01274509207438026	1.203602111674322
>1k	0.001062091006198355	0.2004428148281947
>5k	3.5403033539945167E-4	0.5382209285816713
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9680	0.5331438689831989	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.507684596933872E-5	0.0	0.0	0.0319996475081858	0.0
2	5.507684596933872E-5	0.0	0.0	0.11852537252601693	0.0
3	5.507684596933872E-5	0.0	0.0	0.17442837118489574	0.0
4	5.507684596933872E-5	0.0	0.0	0.2890432876470896	0.0
5	5.507684596933872E-5	0.0	0.0	0.5105623621357699	0.0
6	5.507684596933872E-5	0.0	0.0	0.702615324030854	0.0
7	5.507684596933872E-5	0.0	0.0	0.8173954710309559	0.0
8	5.507684596933872E-5	0.0	0.0	1.050590836865136	0.0
9	5.507684596933872E-5	0.0	0.0	1.1147002855734462	0.0
10	5.507684596933872E-5	0.0	0.0	1.2581203924776043	0.0
11	5.507684596933872E-5	0.0	0.0	1.4944000616860675	0.0
12	5.507684596933872E-5	0.0	0.0	1.693668090403135	0.0
13	5.507684596933872E-5	0.0	0.0	1.7697292146867918	0.0
14	5.507684596933872E-5	0.0	0.0	1.8031608601901803	0.0
15	5.507684596933872E-5	0.0	0.0	1.850251563493965	0.0
16	5.507684596933872E-5	0.0	0.0	1.9490594251629587	0.0
17	5.507684596933872E-5	0.0	0.0	2.075130325586775	0.0
18	5.507684596933872E-5	0.0	0.0	2.241021785646423	0.0
19	5.507684596933872E-5	0.0	0.0	2.3365250365572563	0.0
20	5.507684596933872E-5	0.0	0.0	2.4467888821878727	0.0
21	5.507684596933872E-5	0.0	0.0	2.58778560786938	0.0
22	5.507684596933872E-5	0.0	0.0	2.7293881788565497	0.0
23	5.507684596933872E-5	0.0	0.0	2.8744055142938185	0.0
24	5.507684596933872E-5	0.0	0.0	2.9886899696801965	0.0
25	5.507684596933872E-5	0.0	0.0	3.0850193732805695	0.0
26	5.507684596933872E-5	0.0	0.0	3.188508766856957	0.0
27	5.507684596933872E-5	0.0	0.0	3.3004799947126227	0.0
28	5.507684596933872E-5	0.0	0.0	3.4131121447199204	0.0
29	5.507684596933872E-5	0.0	0.0	3.541991964288173	0.0
30	5.507684596933872E-5	0.0	0.0	3.6947751350071187	0.0
31	5.507684596933872E-5	0.0	0.0	3.8423810822049465	0.0
32	5.507684596933872E-5	0.0	0.0	3.9739045903797274	0.0
33	5.507684596933872E-5	0.0	0.0	4.086371509849117	0.0
34	5.507684596933872E-5	0.0	0.0	4.228304541912103	0.0
35	5.507684596933872E-5	0.0	0.0	4.395793230504862	0.0
36	5.507684596933872E-5	0.0	0.0	4.534256421271779	0.0
37	5.507684596933872E-5	0.0	0.0	4.677951912405784	0.0
38	5.507684596933872E-5	0.0	0.0	4.8440636798493095	0.0
39	5.507684596933872E-5	0.0	0.0	5.091523948789549	0.0
40	5.507684596933872E-5	0.0	0.0	5.259177867920216	0.0
41	5.507684596933872E-5	0.0	0.0	5.429695783041288	0.0
42	5.507684596933872E-5	0.0	0.0	5.599497699164759	0.0
43	5.507684596933872E-5	0.0	0.0	5.748811028587637	0.0
44	5.507684596933872E-5	0.0	0.0	5.893332672411182	0.0
45	5.507684596933872E-5	0.0	0.0	6.049475530734257	0.0
46	5.507684596933872E-5	0.0	0.0	6.2290811254402705	0.0
47	5.507684596933872E-5	0.0	0.0	6.422786392714435	0.0
48	5.507684596933872E-5	0.0	0.0	6.598481531356625	0.0
49	5.507684596933872E-5	0.0	0.0	6.787945881491151	0.0
50	5.507684596933872E-5	0.0	0.0	6.97542746517078	0.0
51	5.507684596933872E-5	0.0	0.0	7.159604438092249	0.0
52	5.507684596933872E-5	0.0	0.0	7.383106279035824	0.0
53	5.507684596933872E-5	0.0	0.0	7.576370931542234	0.0
54	5.507684596933872E-5	0.0	0.0	7.785497715687813	0.0
55	5.507684596933872E-5	0.0	0.0	7.985591897094421	0.0
56	5.507684596933872E-5	0.0	0.0	8.192625761093165	0.0
57	5.507684596933872E-5	0.0	0.0	8.397291320715228	0.0
58	5.507684596933872E-5	0.0	0.0	8.582569830556084	0.0
59	5.507684596933872E-5	0.0	0.0	8.776275097830247	0.0
60	5.507684596933872E-5	0.0	0.0	8.98766003266057	0.0
61	5.507684596933872E-5	0.0	0.0	9.215788328665571	0.0
62	5.507684596933872E-5	0.0	0.0	9.477183039636053	0.0
63	5.507684596933872E-5	0.0	0.0	9.762260794373349	0.0
64	5.507684596933872E-5	0.0	0.0	10.004984454560224	0.0
65	5.507684596933872E-5	0.0	0.0	10.238950896237975	0.0
66	5.507684596933872E-5	0.0	0.0	10.466583500629254	0.0
67	5.507684596933872E-5	0.0	0.0	10.689699803651044	0.0
68	1.1015369193867744E-4	0.0	0.0	10.962054806969425	0.0
69	1.1015369193867744E-4	0.0	0.0	11.331675520269656	0.0
70	1.6523053790801616E-4	5.507684596933872E-5	0.0	11.584918858036676	0.0
71	1.6523053790801616E-4	5.507684596933872E-5	0.0	11.82444806115733	0.0
72	2.203073838773549E-4	5.507684596933872E-5	0.0	12.063922187432015	0.0
73	2.203073838773549E-4	5.507684596933872E-5	0.0	12.303836928474453	0.0
74	2.753842298466936E-4	5.507684596933872E-5	0.0	12.570078401890237	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTACG	55	0.0	72.72727	1
TATAGCG	100	0.0	72.0	1
CGTTTTT	5830	0.0	71.28645	1
TCGATTG	80	0.0	70.0	1
AGGGTCG	150	0.0	66.666664	6
CGATAGG	355	0.0	65.35211	2
CGTTTGG	385	0.0	64.41559	2
CGGGTAC	175	0.0	64.00001	6
GGCCGTA	25	1.2357187E-4	64.0	9
GGGCGAT	1785	0.0	63.64146	7
CGTTAGG	380	0.0	63.157894	2
AGGGATT	2965	0.0	63.13659	6
ACGAGGG	670	0.0	62.686565	3
TACGTAG	115	0.0	62.608696	1
GGTACGA	45	9.427822E-9	62.222225	8
TAGGGTA	1150	0.0	61.913044	5
CGAGGGA	1205	0.0	61.74274	4
GTAGCGG	340	0.0	61.176468	2
TAAGGGA	2355	0.0	60.976646	4
TAGGGAC	1820	0.0	60.87912	5
>>END_MODULE