FastQCFastQC Report
Thu 26 May 2016
SRR1547817_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547817_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3026461
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256040.8460046238824818No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT149000.074.6577151
CGTAAGG6150.065.6910552
TACGGGA13400.065.373144
CCGATCC22900.065.1528480
GCGTAAG3000.064.01
TAGGGCG7750.063.9999965
TAAGGGA44100.063.7641754
CGAGGGA23050.063.6876374
GGGCGAT21800.063.4862377
TTGCGGG22850.062.144423
GTACGGG11050.061.538463
TATGGGA36800.060.760874
TGCGGGA24450.060.20454
AGGGATC38800.060.1030926
TAACGGG10450.059.712923
TAGGGAT49800.059.6787155
GTAGGGA54800.059.6350364
ACGGGAT16200.059.506175
TAGGGAC35000.059.428575
GGCGATA5400.059.259268
Produced by FastQC (version 0.11.5)