##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547817_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3026461
Sequences flagged as poor quality	0
Sequence length	86
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.010702269085904	33.0	31.0	34.0	31.0	34.0
2	32.44996548774294	34.0	31.0	34.0	31.0	34.0
3	32.55604516298079	34.0	31.0	34.0	31.0	34.0
4	36.10113660807127	37.0	35.0	37.0	35.0	37.0
5	36.05998028720674	37.0	35.0	37.0	35.0	37.0
6	36.11268508003242	37.0	36.0	37.0	35.0	37.0
7	36.33851287031288	37.0	37.0	37.0	35.0	37.0
8	36.34624566449064	37.0	37.0	37.0	35.0	37.0
9	38.16022212082032	39.0	39.0	39.0	37.0	39.0
10-11	37.68112128324138	39.0	37.5	39.0	35.0	39.0
12-13	37.13123232052222	39.0	37.0	39.0	33.5	39.0
14-15	38.32904388987666	40.0	37.0	41.0	33.5	41.0
16-17	38.45499545508764	40.0	37.0	41.0	34.0	41.0
18-19	38.27396487184207	40.0	37.0	41.0	34.0	41.0
20-21	38.07418169274278	40.0	35.0	41.0	34.0	41.0
22-23	37.890670819812314	40.0	35.0	41.0	34.0	41.0
24-25	37.85985991559118	40.0	35.0	41.0	34.0	41.0
26-27	37.84535535068848	40.0	35.0	41.0	34.0	41.0
28-29	37.75296063620182	40.0	35.0	41.0	34.0	41.0
30-31	37.574098757591784	40.0	35.0	41.0	33.0	41.0
32-33	37.19820228973709	40.0	35.0	41.0	32.5	41.0
34-35	36.826463813675446	40.0	35.0	41.0	31.0	41.0
36-37	36.61576871468028	40.0	35.0	41.0	31.0	41.0
38-39	36.509336482446	39.5	35.0	41.0	30.5	41.0
40-41	36.38460928457363	39.0	35.0	41.0	30.0	41.0
42-43	36.320570792090166	39.0	35.0	41.0	30.0	41.0
44-45	36.16838892026033	39.0	35.0	41.0	30.0	41.0
46-47	36.01700567098007	39.0	35.0	41.0	29.0	41.0
48-49	35.907099579343665	39.0	35.0	41.0	29.0	41.0
50-51	35.66310188698945	38.0	35.0	40.0	28.5	41.0
52-53	35.45886928660241	38.0	35.0	40.0	28.0	41.0
54-55	35.25320811997908	37.5	35.0	40.0	27.5	41.0
56-57	34.953860796488044	37.0	34.5	40.0	26.5	41.0
58-59	34.68076740456923	36.5	34.0	40.0	26.0	41.0
60-61	34.32443305894244	36.0	34.0	39.0	26.0	41.0
62-63	33.94652384418633	35.5	33.5	39.0	25.5	40.5
64-65	33.58669482276494	35.0	33.0	38.5	24.5	40.0
66-67	33.27718183052747	35.0	33.0	38.0	23.5	40.0
68-69	32.809594936131674	35.0	33.0	37.0	21.5	39.5
70-71	32.50521450631612	35.0	33.0	36.5	20.5	39.0
72-73	32.25303844985942	35.0	33.0	36.0	20.0	39.0
74-75	31.95210924574941	35.0	32.5	36.0	20.0	37.5
76-77	30.03833107381856	33.0	29.5	34.5	17.5	36.0
78-79	31.090391714943625	34.0	31.5	35.0	18.5	36.0
80-81	31.226698609365855	35.0	32.0	35.0	18.5	36.0
82-83	31.042398365615814	34.5	32.0	35.0	18.0	36.0
84-85	30.83805870949601	34.0	31.5	35.0	17.0	36.0
86	30.43590451025141	34.0	31.0	35.0	15.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	6.0
10	11.0
11	21.0
12	38.0
13	130.0
14	486.0
15	1342.0
16	3653.0
17	8796.0
18	19052.0
19	29413.0
20	32901.0
21	30966.0
22	26123.0
23	22310.0
24	21371.0
25	22916.0
26	26508.0
27	31636.0
28	38219.0
29	47491.0
30	58927.0
31	74084.0
32	90846.0
33	117980.0
34	281654.0
35	268210.0
36	267585.0
37	503087.0
38	716261.0
39	283839.0
40	593.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.923960692042623	24.05872733863083	30.443280121567735	15.574031847758818
2	31.6981451272625	25.414502285012098	30.034221488398494	12.853131099326903
3	28.098660448622997	23.89982887603706	34.90314264746844	13.098368027871498
4	26.123713472600507	26.54070216004766	33.41080555804288	13.924778809308958
5	24.34615876431251	30.39527025129351	31.7580500789536	13.50052090544038
6	21.784685148759557	39.787725663737284	27.88150912897936	10.5460800585238
7	87.30874113362108	3.405660935330077	7.768776799040198	1.5168211320086398
8	88.55128151329225	2.5557573680942856	7.440274300577474	1.4526868180359833
9	84.57055947524188	4.818730523869299	8.58553934777286	2.0251706531159663
10-11	36.11234375727954	28.465375896137434	21.78038970269235	13.641890643890669
12-13	27.83779470477234	24.264165307268126	29.61857099761074	18.279468990348793
14-15	18.48399830693341	30.179985798594466	33.469884462413354	17.866131432058765
16-17	23.731463911149028	26.225614670071746	31.63757933771491	18.40534208106432
18-19	24.19897365272508	28.53560643933624	29.681961868994843	17.583458038943835
20-21	26.568226056770598	27.468055924064444	29.797261554006475	16.16645646515848
22-23	22.88058560807491	23.96383102243842	32.20517297265684	20.95041039682983
24-25	20.682655418325233	27.487616724616636	32.77316311031267	19.056564746745455
26-27	20.079326976293434	31.73199654646136	29.3407051999018	18.847971277343404
28-29	18.753521026704128	28.128464235950833	33.6878453084312	19.43016942891384
30-31	22.336765615020315	27.426059678284304	31.10487794159581	19.132296765099564
32-33	24.354848782125394	27.64076589785892	29.018067637415452	18.986317682600237
34-35	21.328806153457787	27.79249426970974	29.940316428990826	20.938383147841655
36-37	22.42635870741437	29.17645395067044	29.140140910456143	19.257046431459056
38-39	21.3106000705114	29.909934408538554	27.846782099620647	20.9326834213294
40-41	21.501251792109663	27.139834281690728	29.135415919782215	22.223498006417397
42-43	21.218974901708627	27.312808590627803	28.30543661391969	23.16277989374388
44-45	20.826040712237827	29.360645982221477	28.305981805151298	21.507331500389398
46-47	21.598461040799798	29.005197820160245	27.88154217087218	21.51479896816777
48-49	21.57249011303962	27.12539497452635	30.38620025171314	20.915914660720887
50-51	20.291753305263143	28.927764144325668	29.43439548700611	21.34608706340508
52-53	22.01493757890817	26.584697440343692	29.502709600421085	21.897655380327055
54-55	21.741036808338187	27.132713753787012	30.028059175386694	21.0981902624881
56-57	22.09251994326046	26.657703502539764	28.99279058940459	22.256985964795184
58-59	22.953542107431748	27.304548117421636	28.566930153734017	21.1749796214126
60-61	23.16172255317349	29.207893311693095	27.31759966508737	20.31278447004604
62-63	23.136015960555913	26.906277662259647	29.249839994633998	20.707866382550442
64-65	21.3356623462189	29.10044107622732	29.30634163136416	20.257554946189625
66-67	20.119951983521347	28.97299849560262	27.118175320944164	23.78887419993187
68-69	20.134622583935492	30.60530104303343	26.87287230861392	22.38720406441715
70-71	20.675171429600447	29.09102413677229	28.66770792684921	21.56609650677805
72-73	22.159776716105046	29.581151053986822	27.20776841333822	21.051303816569913
74-75	21.16518270019009	30.219536944305574	26.414333440939764	22.200946914564568
76-77	23.078572629880245	28.263159512050546	27.301078718675047	21.357189139394166
78-79	19.879208752400906	28.866223618939745	28.968141337357395	22.286426291301954
80-81	19.148669022994184	28.329061567289322	28.934108187748	23.588161221968498
82-83	20.5996541835497	28.371404752944112	28.81553735534672	22.213403708159465
84-85	18.99560245448397	27.996643604526874	28.53869585631535	24.469058084673815
86	20.00359495793932	28.488587825846757	28.745191165523032	22.76262605069089
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1122.0
1	1226.5
2	6674.5
3	7874.0
4	3929.0
5	4513.0
6	5410.5
7	5923.0
8	5983.0
9	6287.0
10	6546.0
11	6723.0
12	7083.0
13	7589.5
14	7898.0
15	8061.5
16	8212.0
17	8333.5
18	8942.5
19	9451.0
20	9480.0
21	9475.0
22	10051.0
23	11238.5
24	13423.5
25	16332.5
26	18660.0
27	21979.5
28	27614.5
29	30922.0
30	35293.5
31	44554.0
32	50330.5
33	55663.5
34	64817.0
35	72912.0
36	76299.0
37	76979.0
38	86970.0
39	99341.5
40	107163.5
41	120113.5
42	131394.5
43	134487.0
44	139167.0
45	148602.0
46	160402.0
47	168595.0
48	169945.0
49	160638.0
50	151129.0
51	136851.5
52	116650.0
53	109744.5
54	101373.0
55	87430.5
56	76941.0
57	73004.0
58	63694.5
59	48438.5
60	36756.5
61	28767.5
62	23455.0
63	17104.0
64	13812.0
65	12104.0
66	9114.0
67	6740.5
68	4942.5
69	3613.0
70	2301.0
71	1612.0
72	1639.5
73	1297.0
74	800.0
75	630.0
76	435.0
77	232.0
78	147.5
79	114.0
80	89.0
81	46.5
82	22.0
83	9.0
84	4.5
85	7.5
86	9.0
87	6.5
88	2.0
89	1.5
90	2.0
91	1.5
92	1.0
93	0.0
94	1.5
95	1.5
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	3026461.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.047876534363063
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.78873065307161	14.400171596601854
2	8.080685335558	2.916784224983779
3	2.6074354695430064	1.4117602027689347
4	1.2636053095780835	0.9122157046172344
5	0.7902403763453331	0.7131080372374585
6	0.5296872850633965	0.5735838433587689
7	0.4122421349250267	0.5208066607372385
8	0.3152140227007747	0.45511550108827975
9	0.28144849419371987	0.457159290659159
>10	3.138825656647859	13.573221848769201
>50	1.2623154858039107	16.492129031054866
>100	1.5208590377280073	45.52268362254034
>500	0.007598729627548154	0.898440363632401
>1k	9.266743448229456E-4	0.2963916627753799
>5k	0.0	0.0
>10k+	1.853348689645891E-4	0.8564284091751301
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25604	0.8460046238824818	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.3041892824655595E-5	0.0	0.0	0.02018859651586457	0.0
2	3.3041892824655595E-5	0.0	0.0	0.08068830227780896	0.0
3	3.3041892824655595E-5	0.0	0.0	0.13986633232676715	0.0
4	3.3041892824655595E-5	0.0	0.0	0.24807853132751423	0.0
5	3.3041892824655595E-5	0.0	0.0	0.4907051503389603	0.0
6	3.3041892824655595E-5	0.0	0.0	0.7322413868871926	0.0
7	3.3041892824655595E-5	0.0	0.0	0.8913381008379093	0.0
8	3.3041892824655595E-5	0.0	0.0	1.1516421325105461	0.0
9	3.3041892824655595E-5	0.0	0.0	1.2575083571207426	0.0
10	3.3041892824655595E-5	0.0	0.0	1.4359345783738828	0.0
11	3.3041892824655595E-5	0.0	0.0	1.7162950389910856	0.0
12	3.3041892824655595E-5	0.0	0.0	1.9192053028272957	0.0
13	3.3041892824655595E-5	0.0	0.0	1.9999596888907538	0.0
14	3.3041892824655595E-5	0.0	0.0	2.0364379385691738	0.0
15	3.3041892824655595E-5	0.0	0.0	2.0877850400186886	0.0
16	3.3041892824655595E-5	0.0	0.0	2.19510510791317	0.0
17	3.3041892824655595E-5	0.0	0.0	2.3293543184597456	0.0
18	3.3041892824655595E-5	0.0	0.0	2.4926143109063688	0.0
19	3.3041892824655595E-5	0.0	0.0	2.6035689870115624	0.0
20	3.3041892824655595E-5	0.0	0.0	2.7230815133583417	0.0
21	3.3041892824655595E-5	0.0	0.0	2.874149047352667	0.0
22	6.608378564931119E-5	0.0	0.0	3.0425635750799365	0.0
23	6.608378564931119E-5	0.0	0.0	3.222774058545608	0.0
24	6.608378564931119E-5	0.0	0.0	3.355569425807899	0.0
25	6.608378564931119E-5	0.0	0.0	3.4814920793626616	0.0
26	6.608378564931119E-5	0.0	0.0	3.5970395785704823	0.0
27	6.608378564931119E-5	0.0	0.0	3.7246473686593022	0.0
28	6.608378564931119E-5	0.0	0.0	3.8572114426718205	0.0
29	6.608378564931119E-5	0.0	0.0	4.0158125282301675	0.0
30	6.608378564931119E-5	0.0	0.0	4.179336855819388	0.0
31	6.608378564931119E-5	0.0	0.0	4.3357241345584825	0.0
32	6.608378564931119E-5	0.0	0.0	4.478894656167715	0.0
33	6.608378564931119E-5	0.0	0.0	4.632341206445416	0.0
34	6.608378564931119E-5	0.0	0.0	4.789058904112758	0.0
35	6.608378564931119E-5	0.0	0.0	4.9694015551497275	0.0
36	6.608378564931119E-5	0.0	0.0	5.131835500275734	0.0
37	9.912567847396678E-5	0.0	0.0	5.307618370102903	0.0
38	1.3216757129862238E-4	0.0	0.0	5.493941603741136	0.0
39	1.3216757129862238E-4	0.0	0.0	5.730851975293916	0.0
40	1.3216757129862238E-4	0.0	0.0	5.932704898559737	0.0
41	1.3216757129862238E-4	0.0	0.0	6.135218659682051	0.0
42	1.3216757129862238E-4	0.0	0.0	6.33819500730391	0.0
43	1.3216757129862238E-4	0.0	0.0	6.533406510111976	0.0
44	1.65209464123278E-4	0.0	0.0	6.7187054450726444	0.0
45	1.9825135694793357E-4	0.0	0.0	6.899741975858932	0.0
46	1.9825135694793357E-4	0.0	0.0	7.103544370801408	0.0
47	2.3129324977258918E-4	0.0	0.0	7.3164663281634885	0.0
48	2.3129324977258918E-4	0.0	0.0	7.523440744817131	0.0
49	2.6433514259724476E-4	0.0	0.0	7.72839960600847	0.0
50	2.6433514259724476E-4	0.0	0.0	7.941189395799252	0.0
51	2.6433514259724476E-4	0.0	0.0	8.151236708485587	0.0
52	2.6433514259724476E-4	0.0	0.0	8.394325913996578	0.0
53	2.9737703542190037E-4	0.0	0.0	8.610816395783722	0.0
54	2.9737703542190037E-4	0.0	0.0	8.828066841105834	0.0
55	3.30418928246556E-4	0.0	0.0	9.044524281000152	0.0
56	3.30418928246556E-4	0.0	0.0	9.274991483452125	0.0
57	3.634608210712116E-4	0.0	0.0	9.49951114519566	0.0
58	3.634608210712116E-4	0.0	0.0	9.718942355444197	0.0
59	4.2954460672052275E-4	0.0	0.0	9.957273528388438	0.0
60	4.2954460672052275E-4	0.0	0.0	10.192928308013881	0.0
61	4.2954460672052275E-4	0.0	0.0	10.44097379744857	0.0
62	4.6258649954517835E-4	0.0	0.0	10.71251207268159	0.0
63	4.956283923698339E-4	0.0	0.0	10.972485685425982	0.0
64	4.956283923698339E-4	0.0	0.0	11.228659480495535	0.0
65	4.956283923698339E-4	0.0	0.0	11.473764241468832	0.0
66	4.956283923698339E-4	0.0	0.0	11.709749440022522	0.0
67	4.956283923698339E-4	0.0	0.0	11.95601066724468	0.0
68	4.956283923698339E-4	0.0	0.0	12.241889123963599	0.0
69	4.956283923698339E-4	0.0	0.0	12.603268305786859	0.0
70	5.947540708438007E-4	0.0	0.0	12.874277910734683	0.0
71	5.947540708438007E-4	0.0	0.0	13.132136842338296	0.0
72	5.947540708438007E-4	0.0	0.0	13.390028815834732	0.0
73	5.947540708438007E-4	0.0	0.0	13.64699561633208	0.0
74	6.277959636684563E-4	0.0	0.0	13.93039593108915	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	14900	0.0	74.657715	1
CGTAAGG	615	0.0	65.691055	2
TACGGGA	1340	0.0	65.37314	4
CCGATCC	2290	0.0	65.15284	80
GCGTAAG	300	0.0	64.0	1
TAGGGCG	775	0.0	63.999996	5
TAAGGGA	4410	0.0	63.764175	4
CGAGGGA	2305	0.0	63.687637	4
GGGCGAT	2180	0.0	63.486237	7
TTGCGGG	2285	0.0	62.14442	3
GTACGGG	1105	0.0	61.53846	3
TATGGGA	3680	0.0	60.76087	4
TGCGGGA	2445	0.0	60.2045	4
AGGGATC	3880	0.0	60.103092	6
TAACGGG	1045	0.0	59.71292	3
TAGGGAT	4980	0.0	59.678715	5
GTAGGGA	5480	0.0	59.635036	4
ACGGGAT	1620	0.0	59.50617	5
TAGGGAC	3500	0.0	59.42857	5
GGCGATA	540	0.0	59.25926	8
>>END_MODULE