FastQCFastQC Report
Thu 26 May 2016
SRR1547816_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547816_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2315432
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201630.8708094213088529No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT25890.11181498744078859No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT113000.073.94691
TACGGGA6650.066.165414
AATCGCG559.094947E-1265.4545441
CCGATCC22150.064.830780
ATAGCGG4950.064.646472
GGGCGAT19650.063.7150087
TTACGGG8100.062.7160453
ACGGGTA3050.061.6393475
CGGGATC6900.061.449286
AGGGATT39850.060.928486
GCGCGAC1250.060.89
TGCGGGA18600.060.645164
TAGGGTA16900.060.355035
TAGTAGG14450.060.346022
ACGAGGG11500.060.1739123
GTAGGGT16450.059.8176274
CGTAAGG5150.059.8058242
TAGGGAT36400.059.6703265
CGGGATT13050.059.4636046
TATGCGG5450.059.449542
Produced by FastQC (version 0.11.5)