##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547816_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2315432
Sequences flagged as poor quality	0
Sequence length	86
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03090827111312	33.0	31.0	34.0	31.0	34.0
2	32.47934078824168	34.0	31.0	34.0	31.0	34.0
3	32.584112597562786	34.0	31.0	34.0	31.0	34.0
4	36.119600575616126	37.0	35.0	37.0	35.0	37.0
5	36.08295644182166	37.0	35.0	37.0	35.0	37.0
6	36.1247257531208	37.0	36.0	37.0	35.0	37.0
7	36.34886492023951	37.0	37.0	37.0	35.0	37.0
8	36.362824734218066	37.0	37.0	37.0	35.0	37.0
9	38.18406370819787	39.0	39.0	39.0	37.0	39.0
10-11	37.69177285275491	39.0	37.5	39.0	35.0	39.0
12-13	37.032412094157806	39.0	35.0	39.0	33.5	39.0
14-15	38.15577525057959	40.0	36.0	41.0	33.0	41.0
16-17	38.3070388160827	40.0	36.0	41.0	34.0	41.0
18-19	38.14753035286719	40.0	36.0	41.0	34.0	41.0
20-21	37.946204207249444	40.0	35.0	41.0	34.0	41.0
22-23	37.78167918556883	40.0	35.0	41.0	34.0	41.0
24-25	37.731868178378804	40.0	35.0	41.0	34.0	41.0
26-27	37.73363566712389	40.0	35.0	41.0	34.0	41.0
28-29	37.65795216616165	40.0	35.0	41.0	34.0	41.0
30-31	37.4565232319498	40.0	35.0	41.0	33.0	41.0
32-33	37.09473113440602	40.0	35.0	41.0	32.5	41.0
34-35	36.702032061403656	40.0	35.0	41.0	31.0	41.0
36-37	36.49715884551998	39.0	35.0	41.0	31.0	41.0
38-39	36.391785636546444	39.0	35.0	41.0	30.0	41.0
40-41	36.27878102228871	39.0	35.0	41.0	30.0	41.0
42-43	36.232474976591845	39.0	35.0	41.0	30.0	41.0
44-45	36.079111802894666	39.0	35.0	41.0	30.0	41.0
46-47	35.95449013402251	38.5	35.0	41.0	30.0	41.0
48-49	35.84562470415888	38.0	35.0	40.5	29.0	41.0
50-51	35.62417013326239	38.0	35.0	40.0	29.0	41.0
52-53	35.41519530696648	37.5	35.0	40.0	28.0	41.0
54-55	35.21683685808955	37.0	35.0	40.0	28.0	41.0
56-57	34.90394405881926	36.5	34.5	40.0	27.5	41.0
58-59	34.64336957423064	36.0	34.0	39.5	26.5	41.0
60-61	34.28351663965947	35.5	34.0	39.0	26.0	41.0
62-63	33.89882622335702	35.0	33.5	39.0	26.0	40.0
64-65	33.56721294341617	35.0	33.0	38.0	25.0	40.0
66-67	33.26677095246157	35.0	33.0	37.5	24.5	40.0
68-69	32.817380514737636	35.0	33.0	37.0	22.5	39.5
70-71	32.55073999150051	35.0	33.0	36.5	22.5	39.0
72-73	32.3116666781836	35.0	33.0	36.0	21.5	38.5
74-75	32.027838865490324	35.0	33.0	35.5	20.0	37.5
76-77	30.130116755750116	33.0	30.0	34.5	18.5	35.5
78-79	31.190725963880606	34.0	31.5	35.0	19.0	36.0
80-81	31.3341689585356	35.0	32.0	35.0	19.0	36.0
82-83	31.158135933164957	34.5	32.0	35.0	18.5	36.0
84-85	30.962460137028426	34.0	32.0	35.0	18.0	35.5
86	30.570153647353926	34.0	31.0	35.0	17.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	15.0
11	15.0
12	29.0
13	88.0
14	315.0
15	1071.0
16	2799.0
17	6334.0
18	14275.0
19	21548.0
20	23898.0
21	23026.0
22	19714.0
23	16768.0
24	16071.0
25	17443.0
26	20093.0
27	23822.0
28	29080.0
29	35929.0
30	45419.0
31	56445.0
32	71284.0
33	93847.0
34	238362.0
35	217129.0
36	205523.0
37	388048.0
38	523379.0
39	203287.0
40	370.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.365951580525795	24.756460133573345	31.607103987506434	16.270484298394425
2	30.993568370826697	25.532643584436943	31.254685950613105	12.219102094123256
3	27.968430945067702	24.458589153125637	34.778736754091675	12.794243147714985
4	25.834487905496683	26.375898752371047	33.63691958995125	14.152693752181019
5	24.087600067719546	30.81390427358696	31.21572993722122	13.882765721472278
6	21.6789350756144	39.421023808947965	28.057874297323348	10.842166818114288
7	87.5983401801478	3.479134779168639	7.572453002290717	1.3500720383928355
8	89.36207152704118	2.18201182327963	7.132319152538273	1.3235974971409223
9	85.31954296217725	4.576208672938787	8.228270145700673	1.8759782191832886
10-11	38.87555324449174	25.5972967463523	22.28519343258623	13.24195657656973
12-13	29.270369417024554	23.761570195108302	28.60146616268584	18.366594225181306
14-15	18.833224210428117	29.095628807064944	34.35570554436494	17.715441438141998
16-17	24.580704594218272	24.64095253067246	31.568990149570357	19.209352725538906
18-19	25.407807268794762	27.868514385220557	28.807540018450123	17.916138327534558
20-21	26.805365046349884	27.19635040027088	29.04691219608263	16.951372357296606
22-23	23.118774379899733	23.568323319363298	32.1525529577202	21.16034934301677
24-25	21.840503197675424	26.355880889613687	32.30742686461965	19.496189048091242
26-27	19.528407657836638	30.643029033027098	29.854256138811248	19.97430717032502
28-29	19.301970431435688	27.509099813771254	33.68388706729457	19.505042687498488
30-31	23.76018816359107	25.956797694771428	31.197180482950916	19.085833658686585
32-33	26.077401538892094	26.373221066306417	28.715116660735447	18.834260734066042
34-35	21.251239509517013	27.7036207498212	30.092008748259502	20.953130992402283
36-37	23.626066323692513	29.15166586624008	28.568556537181834	18.65371127288558
38-39	22.14703346934827	29.223704259075628	27.341658057762007	21.287604213814095
40-41	22.00729712641097	26.230353558212894	28.80786393208697	22.954485383289168
42-43	21.778009460005734	26.53098860169506	28.327435225910325	23.363566712388874
44-45	21.74369620874204	28.3776202453797	28.237452017593263	21.641231528285
46-47	22.35232993238411	28.339506407443622	28.157682885958213	21.150480774214056
48-49	22.338358457514623	26.24449778702203	30.259731229420687	21.157412526042656
50-51	20.189450607921113	28.781454173562427	29.157841819582696	21.871253398933764
52-53	22.51059413534926	25.589976298159478	29.61812309754724	22.281306468944024
54-55	22.380208099395706	25.754740368104095	30.373705641107147	21.491345891393053
56-57	22.624611735520627	26.110958991669804	28.924494435595605	22.339934837213963
58-59	23.046779175549098	27.68064447584727	27.98767141509662	21.284904933507008
60-61	25.156925359932835	27.8684927909781	26.73250607230098	20.24207577678809
62-63	23.835076996430903	25.4722228940431	29.25492953366802	21.437770575857982
64-65	22.24522248979888	28.261443220962658	29.092454453423812	20.400879835814656
66-67	21.478389345919034	28.84204761789593	26.370025118422824	23.309537917762217
68-69	20.24116881860491	30.257161514568338	27.273528222811123	22.22814144401563
70-71	21.035037954040543	28.363886307177232	28.853125464276214	21.74795027450601
72-73	23.02708522642859	29.330638947721205	26.97455593599812	20.667719889852087
74-75	21.512529843243076	30.40650729539887	26.00292299665894	22.078039864699115
76-77	23.671586986791233	27.77477377871602	27.19835866481935	21.35528056967339
78-79	20.626777206154188	28.55326781352249	28.227453883335805	22.592501096987515
80-81	19.322873658133773	28.11382497952866	28.724510156204115	23.838791206133454
82-83	20.85507153740641	28.030600769100538	29.203556830863526	21.910770862629523
84-85	19.337644119974158	27.210991296656523	28.538842859561413	24.912521723807913
86	20.40889129976609	26.919771342885472	29.756606974422052	22.914730382926383
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	965.0
1	1082.0
2	5656.0
3	6560.5
4	3229.0
5	3748.0
6	4355.0
7	4664.0
8	4809.0
9	5107.5
10	5375.5
11	5500.0
12	5629.5
13	5797.0
14	5845.0
15	6057.0
16	6201.0
17	6224.5
18	6423.0
19	6772.0
20	7207.0
21	7400.0
22	7695.5
23	8307.5
24	9362.0
25	11099.5
26	13460.0
27	15691.5
28	18329.5
29	20097.0
30	22853.5
31	27308.0
32	31655.5
33	35848.5
34	39930.0
35	44608.0
36	46748.0
37	51842.5
38	58913.0
39	67734.0
40	76428.0
41	86990.0
42	97921.5
43	100140.0
44	105854.5
45	114840.0
46	121763.5
47	126238.5
48	130431.5
49	127226.0
50	120650.0
51	110573.5
52	95941.0
53	87326.0
54	82996.0
55	80076.5
56	72254.5
57	67081.0
58	60836.0
59	47323.0
60	35193.5
61	27033.0
62	20341.0
63	14645.5
64	12343.0
65	10326.0
66	7361.5
67	5219.0
68	3219.5
69	2118.5
70	1409.5
71	997.0
72	1000.0
73	695.0
74	365.5
75	247.5
76	103.0
77	75.5
78	77.0
79	58.0
80	44.5
81	22.5
82	11.5
83	10.5
84	10.5
85	11.0
86	13.0
87	10.0
88	4.5
89	1.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2315432.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.265044249744385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.68900461897277	15.352122002025952
2	7.207409220395054	2.777021151138528
3	2.5027823049966305	1.4464863555971197
4	1.2848817703245934	0.9901321664397276
5	0.816327427511839	0.786329200664779
6	0.6149037713420468	0.710768901854354
7	0.44061204736580906	0.5941887412630946
8	0.378727936113915	0.5836968358279149
9	0.3135745118854893	0.5436924162359352
>10	4.019147759608501	18.79263157121609
>50	1.505434078626292	20.91847790802391
>100	1.2224502441470093	34.82305350048105
>500	0.003388791935768561	0.44498410447668835
>1k	0.0011295973119228538	0.3588511288546846
>5k	0.0	0.0
>10k+	2.2591946238457078E-4	0.8775640159001825
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20163	0.8708094213088529	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2589	0.11181498744078859	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2956545474019534E-4	0.0	0.0	0.0111426291076568	0.0
2	1.2956545474019534E-4	0.0	0.0	0.06227779524512057	0.0
3	1.2956545474019534E-4	0.0	0.0	0.11332658441275753	0.0
4	1.2956545474019534E-4	0.0	0.0	0.20009225060377503	0.0
5	1.2956545474019534E-4	0.0	0.0	0.4005731975717706	0.0
6	1.2956545474019534E-4	0.0	0.0	0.6092167681883985	0.0
7	1.2956545474019534E-4	0.0	0.0	0.7439648411182017	0.0
8	1.2956545474019534E-4	0.0	0.0	0.9661264075127233	0.0
9	1.2956545474019534E-4	0.0	0.0	1.0632573100829565	0.0
10	1.2956545474019534E-4	0.0	0.0	1.2405892291373706	0.0
11	1.2956545474019534E-4	0.0	0.0	1.5214439465292007	0.0
12	1.2956545474019534E-4	0.0	0.0	1.7401504341306504	0.0
13	1.2956545474019534E-4	0.0	0.0	1.820869712433792	0.0
14	1.2956545474019534E-4	0.0	0.0	1.8606031185541185	0.0
15	1.2956545474019534E-4	0.0	0.0	1.905303200439486	0.0
16	1.2956545474019534E-4	0.0	0.0	2.0079190405937206	0.0
17	1.2956545474019534E-4	0.0	0.0	2.1337702856313636	0.0
18	1.2956545474019534E-4	0.0	0.0	2.282597804642935	0.0
19	1.2956545474019534E-4	0.0	0.0	2.3900939435923836	0.0
20	1.2956545474019534E-4	0.0	0.0	2.5111512668046396	0.0
21	1.2956545474019534E-4	0.0	0.0	2.6598924088463836	0.0
22	1.2956545474019534E-4	0.0	0.0	2.825217929094873	0.0
23	1.2956545474019534E-4	0.0	0.0	2.9857063390330616	0.0
24	1.2956545474019534E-4	0.0	0.0	3.1037404683013796	0.0
25	1.2956545474019534E-4	0.0	0.0	3.2127913927077105	0.0
26	1.2956545474019534E-4	0.0	0.0	3.3246495686334128	0.0
27	1.2956545474019534E-4	0.0	0.0	3.4547332851925687	0.0
28	1.2956545474019534E-4	0.0	0.0	3.5861990332689535	0.0
29	1.2956545474019534E-4	0.0	0.0	3.7339036516727764	0.0
30	1.2956545474019534E-4	0.0	0.0	3.8837245058373555	0.0
31	1.2956545474019534E-4	0.0	0.0	4.027758103023539	0.0
32	1.2956545474019534E-4	0.0	0.0	4.162678929892996	0.0
33	1.2956545474019534E-4	0.0	0.0	4.292158007663365	0.0
34	1.2956545474019534E-4	0.0	0.0	4.443404081830086	0.0
35	1.2956545474019534E-4	0.0	0.0	4.602337706311393	0.0
36	1.2956545474019534E-4	0.0	0.0	4.751337979262617	0.0
37	1.2956545474019534E-4	0.0	0.0	4.920032201334352	0.0
38	1.2956545474019534E-4	0.0	0.0	5.126257216795829	0.0
39	1.2956545474019534E-4	0.0	0.0	5.469432917917693	0.0
40	1.2956545474019534E-4	0.0	0.0	5.682093017631267	0.0
41	1.2956545474019534E-4	0.0	0.0	5.892032242795297	0.0
42	1.2956545474019534E-4	0.0	0.0	6.095838703101625	0.0
43	1.727539396535938E-4	0.0	0.0	6.267426553662556	0.0
44	1.727539396535938E-4	0.0	0.0	6.437330053311865	0.0
45	1.727539396535938E-4	0.0	0.0	6.600496149314685	0.0
46	1.727539396535938E-4	0.0	0.0	6.797565205974522	0.0
47	1.727539396535938E-4	0.0	0.0	7.005474572347622	0.0
48	1.727539396535938E-4	0.0	0.0	7.195935790815709	0.0
49	1.727539396535938E-4	0.0	0.0	7.395812098994917	0.0
50	1.727539396535938E-4	0.0	0.0	7.587525783525494	0.0
51	2.1594242456699224E-4	0.0	0.0	7.802258930514911	0.0
52	2.1594242456699224E-4	0.0	0.0	8.059489546659112	0.0
53	2.591309094803907E-4	0.0	0.0	8.2560835299849	0.0
54	2.591309094803907E-4	0.0	0.0	8.455312010890408	0.0
55	2.591309094803907E-4	0.0	0.0	8.645989171783063	0.0
56	2.591309094803907E-4	0.0	0.0	8.871303497576262	0.0
57	2.591309094803907E-4	0.0	0.0	9.087245922143255	0.0
58	2.591309094803907E-4	0.0	0.0	9.297487466701678	0.0
59	2.591309094803907E-4	0.0	0.0	9.509283796716984	0.0
60	2.591309094803907E-4	0.0	0.0	9.7317476825059	0.0
61	2.591309094803907E-4	0.0	0.0	9.967470433163228	0.0
62	2.591309094803907E-4	0.0	0.0	10.2803710063608	0.0
63	2.591309094803907E-4	0.0	0.0	10.555395278289321	0.0
64	2.591309094803907E-4	0.0	0.0	10.80420413987541	0.0
65	2.591309094803907E-4	0.0	0.0	11.040013267502566	0.0
66	2.591309094803907E-4	0.0	0.0	11.266104986024207	0.0
67	2.591309094803907E-4	0.0	0.0	11.500532082134134	0.0
68	2.591309094803907E-4	0.0	0.0	11.78821058014228	0.0
69	2.591309094803907E-4	0.0	0.0	12.244928808101468	0.0
70	3.0231939439378917E-4	0.0	0.0	12.520514530333864	0.0
71	3.0231939439378917E-4	0.0	0.0	12.780897905876744	0.0
72	3.0231939439378917E-4	0.0	0.0	13.028842997764563	0.0
73	3.0231939439378917E-4	0.0	0.0	13.279120267837708	0.0
74	3.455078793071876E-4	0.0	0.0	13.569174132516093	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	11300	0.0	73.9469	1
TACGGGA	665	0.0	66.16541	4
AATCGCG	55	9.094947E-12	65.454544	1
CCGATCC	2215	0.0	64.8307	80
ATAGCGG	495	0.0	64.64647	2
GGGCGAT	1965	0.0	63.715008	7
TTACGGG	810	0.0	62.716045	3
ACGGGTA	305	0.0	61.639347	5
CGGGATC	690	0.0	61.44928	6
AGGGATT	3985	0.0	60.92848	6
GCGCGAC	125	0.0	60.8	9
TGCGGGA	1860	0.0	60.64516	4
TAGGGTA	1690	0.0	60.35503	5
TAGTAGG	1445	0.0	60.34602	2
ACGAGGG	1150	0.0	60.173912	3
GTAGGGT	1645	0.0	59.817627	4
CGTAAGG	515	0.0	59.805824	2
TAGGGAT	3640	0.0	59.670326	5
CGGGATT	1305	0.0	59.463604	6
TATGCGG	545	0.0	59.44954	2
>>END_MODULE