FastQCFastQC Report
Thu 26 May 2016
SRR1547815_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547815_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1589241
Sequences flagged as poor quality0
Sequence length86
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82840.5212551148630069No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT44900.072.694881
CGAGGGA7600.071.578954
TACGCGG451.1823431E-1071.1111152
TCGCACG303.7962527E-666.6666641
TAGGGTA10000.066.45
TTGCGGG11950.065.60673
TTTAGCG1600.065.01
TATTGCG2350.064.6808551
AGGGATT27950.064.400716
TTAGCGG3900.063.589742
CGTAAGG3300.063.03032
TAGGGAT24000.062.05
TAGCGCG1300.061.538461
AATGCGG3400.061.176472
TACGGGA7150.060.9790234
TGGTACG1250.060.81
TAGCGGG13600.060.0000043
CCGATCC9350.059.8930580
GCGCGAC1150.059.1304369
TCGATGG2300.059.1304362
Produced by FastQC (version 0.11.5)