##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547815_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1589241
Sequences flagged as poor quality	0
Sequence length	86
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03919732752931	33.0	31.0	34.0	31.0	34.0
2	32.473562537085314	34.0	31.0	34.0	31.0	34.0
3	32.592951603941756	34.0	31.0	34.0	31.0	34.0
4	36.11025640541617	37.0	35.0	37.0	35.0	37.0
5	36.06355549598834	37.0	35.0	37.0	35.0	37.0
6	36.12922709645674	37.0	36.0	37.0	35.0	37.0
7	36.33994151925353	37.0	37.0	37.0	35.0	37.0
8	36.3464578374205	37.0	37.0	37.0	35.0	37.0
9	38.14808452588374	39.0	39.0	39.0	37.0	39.0
10-11	37.6898909605277	39.0	37.5	39.0	35.0	39.0
12-13	36.84551304679404	39.0	35.0	39.0	33.5	39.0
14-15	37.94021422804974	40.0	35.0	41.0	33.0	41.0
16-17	38.09746004539274	40.0	35.0	41.0	34.0	41.0
18-19	37.96791864795837	40.0	36.0	41.0	34.0	41.0
20-21	37.78721918198687	40.0	35.0	41.0	34.0	41.0
22-23	37.606093411886555	39.5	35.0	41.0	33.0	41.0
24-25	37.57670233778263	39.0	35.0	41.0	34.0	41.0
26-27	37.55977601886687	40.0	35.0	41.0	34.0	41.0
28-29	37.49902154550506	40.0	35.0	41.0	33.0	41.0
30-31	37.33866921379451	39.0	35.0	41.0	33.0	41.0
32-33	37.048917376282134	39.0	35.0	41.0	32.5	41.0
34-35	36.76808898084054	39.0	35.0	41.0	32.0	41.0
36-37	36.57459535715476	39.0	35.0	41.0	31.0	41.0
38-39	36.496410236081246	39.0	35.0	41.0	31.0	41.0
40-41	36.38396536459857	39.0	35.0	41.0	31.0	41.0
42-43	36.32411950107001	39.0	35.0	41.0	31.0	41.0
44-45	36.184631217040085	38.0	35.0	41.0	30.5	41.0
46-47	36.05141416563001	38.0	35.0	41.0	30.0	41.0
48-49	35.964446235655885	38.0	35.0	40.0	30.0	41.0
50-51	35.73255283496965	37.5	35.0	40.0	30.0	41.0
52-53	35.54407009383725	37.0	35.0	40.0	29.0	41.0
54-55	35.35309717028443	37.0	35.0	40.0	29.0	41.0
56-57	35.04444543023997	36.0	34.5	40.0	28.5	41.0
58-59	34.78269029052233	36.0	34.0	39.5	28.0	41.0
60-61	34.429904904290794	35.0	34.0	39.0	27.0	41.0
62-63	34.02015207259314	35.0	33.5	39.0	26.0	40.0
64-65	33.69896541808322	35.0	33.5	38.0	26.0	40.0
66-67	33.40800797361759	35.0	33.0	37.0	25.5	40.0
68-69	32.9940377198927	35.0	33.0	37.0	24.5	39.0
70-71	32.70550690549766	35.0	33.0	36.0	24.0	39.0
72-73	32.47599105484946	35.0	33.0	36.0	24.0	38.5
74-75	32.209331372648954	35.0	33.0	35.0	23.5	37.5
76-77	30.28233162874605	33.0	30.0	34.5	19.5	35.5
78-79	31.277383983926917	34.0	31.5	35.0	20.0	36.0
80-81	31.51424390636788	35.0	32.0	35.0	20.0	36.0
82-83	31.34549700139878	34.5	32.0	35.0	19.5	36.0
84-85	31.15660211383925	34.0	32.0	35.0	19.0	35.5
86	30.755108256079474	34.0	31.0	35.0	18.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	13.0
11	20.0
12	32.0
13	88.0
14	220.0
15	561.0
16	1586.0
17	3538.0
18	6866.0
19	10425.0
20	12824.0
21	13212.0
22	11981.0
23	10966.0
24	10978.0
25	12183.0
26	14439.0
27	17172.0
28	20854.0
29	25632.0
30	32291.0
31	40483.0
32	50897.0
33	68473.0
34	190462.0
35	171055.0
36	134563.0
37	252388.0
38	342655.0
39	132007.0
40	372.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.23669789541045	23.612403656839966	31.76421952365941	14.38667892409018
2	31.30670552798474	25.663193939748595	30.37456244836372	12.655538083902945
3	28.93519610933773	25.220466876955726	32.86078071230229	12.983556301404256
4	27.126282294504104	26.316587603768088	32.04026324515917	14.516866856568639
5	23.9764768213254	31.04047781299375	30.572896118335734	14.410149247345117
6	22.249551830087444	40.98440702196835	26.32457883983612	10.441462308108084
7	87.62151240749515	3.729264472789212	6.776127723863152	1.8730953958524854
8	89.30105629039271	2.5711015509919517	6.426401030428991	1.701441128186348
9	85.05022208714726	4.951671898723982	7.438078932018493	2.560027082110265
10-11	42.04925495881367	25.811000345447923	19.287099942677038	12.852644753061368
12-13	30.802880116986664	25.596558357102538	26.281728196038234	17.31883332987256
14-15	17.44247725801184	30.4558905792136	34.19336651898611	17.90826564378845
16-17	22.747777083526035	24.47234245781477	32.07141018888891	20.708470269770288
18-19	25.30623108767015	27.25989953694877	28.784275009265432	18.64959436611565
20-21	27.774736493709888	27.432120112682725	27.922888976561765	16.870254417045622
22-23	23.140700497910636	24.42515011883031	30.402752005517097	22.031397377741953
24-25	21.04658764781427	26.003828242538418	33.71521374039557	19.23437036925174
26-27	19.247804455082644	32.43683620042523	28.967538592321745	19.347820752170374
28-29	17.946868977077738	27.68208220150374	34.63433173445689	19.73671708696164
30-31	23.88913953264483	27.653420720960508	29.0640311947653	19.393408551629364
32-33	26.812516163376106	26.94446594317665	27.79002681154086	18.452991081906394
34-35	20.965762901913553	26.88528675009014	30.20898026164691	21.939970086349394
36-37	23.514023360837026	28.98732791313589	28.7940910157742	18.70455771025288
38-39	21.734180026817835	29.755430422446942	27.0491700126035	21.461219538131722
40-41	22.27258169151186	25.48660020726875	28.73195443611133	23.508863665108063
42-43	21.849927103567047	26.11089192891449	28.007552032699884	24.03162893481857
44-45	21.256750864091728	29.349607768739922	27.26999869749144	22.123642669676908
46-47	21.94245554953591	29.00154853794988	27.342297360815635	21.713698551698577
48-49	21.797606530412946	25.87348300226334	30.997438399839922	21.331472067483787
50-51	20.195898545280418	29.55071005593236	28.501215360036646	21.752176038750573
52-53	22.97697454319389	25.340303956416932	29.92604645865542	21.756675041733757
54-55	22.983927547804267	25.8834248550094	29.956815863673285	21.175831733513043
56-57	23.450722703479208	24.825718692130394	28.76860715272259	22.95495145166781
58-59	24.255761083435427	26.622991730014515	27.946610992291287	21.17463619425877
60-61	25.11220765132538	27.87154371174668	26.715520175983375	20.300728460944566
62-63	23.624264664704725	25.123533812681654	29.997778813911797	21.254422708701824
64-65	20.64287291858189	29.482753087794737	29.533091582711496	20.341282410911877
66-67	20.384951055252163	28.233980875147317	26.005558628301184	25.375509441299336
68-69	19.69987560099444	30.564558805115144	25.86209391778843	23.873471676101985
70-71	20.39722106338812	28.962032819440225	28.843800279504496	21.796945837667163
72-73	22.680229115659614	29.96458686882606	26.71470217544098	20.640481840073342
74-75	22.254963218290996	31.263917807305504	24.572201447105883	21.90891752729762
76-77	25.490753133099386	27.43422803715736	26.06788397731999	21.007134852423263
78-79	19.81124952099776	28.448171171018117	29.43109320738642	22.309486100597706
80-81	18.582235167605166	27.88607895215389	29.284985725890532	24.24670015435041
82-83	19.882761645338874	27.376244383325123	29.97314441296191	22.767849558374092
84-85	18.24773586888332	26.54839637285975	29.045059874493546	26.15880788376338
86	19.66083180587463	26.974700501686026	29.860795184619576	23.50367250781977
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	631.0
1	895.5
2	2995.5
3	3180.5
4	1634.0
5	1945.0
6	2231.5
7	2311.0
8	2462.5
9	2610.5
10	2661.5
11	2717.5
12	2834.0
13	2968.0
14	2987.0
15	2979.5
16	3114.5
17	3287.5
18	3360.5
19	3439.0
20	3895.0
21	4315.0
22	4663.0
23	5461.5
24	6299.5
25	7569.0
26	9267.5
27	10666.5
28	12935.5
29	14622.0
30	15407.5
31	18763.0
32	21773.0
33	23773.5
34	27510.0
35	31261.0
36	32836.0
37	34014.5
38	38366.5
39	47082.0
40	56204.0
41	64433.5
42	72120.0
43	75157.0
44	78485.5
45	84029.0
46	88694.0
47	95831.0
48	99523.5
49	91126.5
50	83724.0
51	74117.0
52	60018.5
53	55204.5
54	53497.5
55	49597.0
56	44845.5
57	42610.0
58	39347.0
59	30410.5
60	21283.5
61	16946.5
62	14087.0
63	10381.5
64	8652.0
65	7087.5
66	4707.0
67	3514.0
68	2754.5
69	2006.0
70	1518.5
71	1397.0
72	1140.0
73	758.5
74	509.0
75	259.5
76	164.0
77	128.0
78	51.0
79	39.0
80	39.0
81	29.0
82	17.5
83	12.0
84	8.0
85	6.5
86	5.0
87	2.5
88	1.5
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1589241.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.51121860325866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.70858710159625	13.957944932929353
2	8.20273732848082	2.8727985300827514
3	2.4983450497137447	1.312471989357195
4	1.24521525378958	0.8722094606888617
5	0.7029564813084245	0.6154812306384664
6	0.45510590757802544	0.478167542113994
7	0.37028194041334384	0.4538861602391802
8	0.2824478879797428	0.39568053683535914
9	0.23506304510839152	0.3704616331596626
>10	3.1630480686964106	13.682631106820903
>50	1.4754163095321529	18.690274610164174
>100	1.654628407446098	44.98781616461762
>500	0.005078885705820076	0.6373005556051345
>1k	7.255551008314394E-4	0.14661978492191494
>5k	3.627775504157197E-4	0.5262557618254521
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8284	0.5212551148630069	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.292311864594483E-5	0.0	0.0	0.020953398509099625	0.0
2	6.292311864594483E-5	0.0	0.0	0.1118773049524899	0.0
3	6.292311864594483E-5	0.0	0.0	0.18159612041219678	0.0
4	6.292311864594483E-5	0.0	0.0	0.3203415970265051	0.0
5	6.292311864594483E-5	0.0	0.0	0.6191005643574511	0.0
6	6.292311864594483E-5	0.0	0.0	0.8624242641613198	0.0
7	6.292311864594483E-5	0.0	0.0	1.0126217483691902	0.0
8	6.292311864594483E-5	0.0	0.0	1.301250093598139	0.0
9	6.292311864594483E-5	0.0	0.0	1.3921110769228833	0.0
10	6.292311864594483E-5	0.0	0.0	1.5789927393013394	0.0
11	6.292311864594483E-5	0.0	0.0	1.9265800467015386	0.0
12	6.292311864594483E-5	0.0	0.0	2.196771918167226	0.0
13	6.292311864594483E-5	0.0	0.0	2.2943656751870862	0.0
14	6.292311864594483E-5	0.0	0.0	2.3316790845441315	0.0
15	6.292311864594483E-5	0.0	0.0	2.3940358951222627	0.0
16	6.292311864594483E-5	0.0	0.0	2.5244125969566604	0.0
17	6.292311864594483E-5	0.0	0.0	2.6839227027241304	0.0
18	6.292311864594483E-5	0.0	0.0	2.872377443068735	0.0
19	6.292311864594483E-5	0.0	0.0	2.9997338352081275	0.0
20	6.292311864594483E-5	0.0	0.0	3.145212085517552	0.0
21	6.292311864594483E-5	0.0	0.0	3.3340443645740323	0.0
22	6.292311864594483E-5	0.0	0.0	3.5302386485120887	0.0
23	6.292311864594483E-5	0.0	0.0	3.7181900039075257	0.0
24	1.2584623729188966E-4	0.0	0.0	3.8573759423523555	0.0
25	1.2584623729188966E-4	0.0	0.0	3.983788487712059	0.0
26	1.2584623729188966E-4	0.0	0.0	4.113535958359997	0.0
27	1.2584623729188966E-4	0.0	0.0	4.2422766591096	0.0
28	1.2584623729188966E-4	0.0	0.0	4.381399674435784	0.0
29	1.2584623729188966E-4	0.0	0.0	4.544559321084719	0.0
30	1.2584623729188966E-4	0.0	0.0	4.719233898445862	0.0
31	1.2584623729188966E-4	0.0	0.0	4.884344161772821	0.0
32	1.2584623729188966E-4	0.0	0.0	5.035674262116318	0.0
33	1.2584623729188966E-4	0.0	0.0	5.191975288832845	0.0
34	1.2584623729188966E-4	0.0	0.0	5.3625598634820015	0.0
35	1.2584623729188966E-4	0.0	0.0	5.5506370651147305	0.0
36	1.2584623729188966E-4	0.0	0.0	5.725248719357228	0.0
37	1.2584623729188966E-4	0.0	0.0	5.911438227430579	0.0
38	1.2584623729188966E-4	0.0	0.0	6.1156866705553155	0.0
39	1.2584623729188966E-4	0.0	0.0	6.3928630081907025	0.0
40	1.2584623729188966E-4	0.0	0.0	6.603340840061388	0.0
41	1.2584623729188966E-4	0.0	0.0	6.824767294576468	0.0
42	1.2584623729188966E-4	0.0	0.0	7.0384542054980965	0.0
43	1.2584623729188966E-4	0.0	0.0	7.218414324825498	0.0
44	1.2584623729188966E-4	0.0	0.0	7.391767516695077	0.0
45	1.2584623729188966E-4	0.0	0.0	7.567952248903722	0.0
46	1.2584623729188966E-4	0.0	0.0	7.787553932978069	0.0
47	1.8876935593783447E-4	0.0	0.0	8.025466244578388	0.0
48	1.8876935593783447E-4	0.0	0.0	8.231036073194689	0.0
49	1.8876935593783447E-4	0.0	0.0	8.441136366353499	0.0
50	1.8876935593783447E-4	0.0	0.0	8.653061429953041	0.0
51	1.8876935593783447E-4	0.0	0.0	8.860770644603305	0.0
52	1.8876935593783447E-4	0.0	0.0	9.095033415322156	0.0
53	1.8876935593783447E-4	0.0	0.0	9.300099858989292	0.0
54	1.8876935593783447E-4	0.0	0.0	9.519449850589055	0.0
55	1.8876935593783447E-4	0.0	0.0	9.737730149171837	0.0
56	1.8876935593783447E-4	0.0	0.0	9.981997695755394	0.0
57	1.8876935593783447E-4	0.0	0.0	10.203424150270475	0.0
58	3.146155932297241E-4	0.0	0.0	10.415726752581893	0.0
59	3.146155932297241E-4	0.0	0.0	10.636083514079992	0.0
60	3.146155932297241E-4	0.0	0.0	10.876827366019377	0.0
61	3.146155932297241E-4	0.0	0.0	11.120969066365642	0.0
62	3.146155932297241E-4	0.0	0.0	11.407709718035212	0.0
63	3.146155932297241E-4	0.0	0.0	11.69979883478969	0.0
64	3.146155932297241E-4	0.0	0.0	11.950358693237842	0.0
65	3.146155932297241E-4	0.0	0.0	12.194689162940046	0.0
66	3.146155932297241E-4	0.0	0.0	12.420142697048465	0.0
67	3.146155932297241E-4	0.0	0.0	12.661264087699726	0.0
68	3.146155932297241E-4	0.0	0.0	12.936741501131673	0.0
69	3.146155932297241E-4	0.0	0.0	13.318244369482036	0.0
70	3.146155932297241E-4	0.0	0.0	13.589317164608767	0.0
71	3.7753871187566894E-4	0.0	0.0	13.837548867667019	0.0
72	4.404618305216138E-4	0.0	0.0	14.074706101843585	0.0
73	4.404618305216138E-4	0.0	0.0	14.324636729105277	0.0
74	4.404618305216138E-4	0.0	0.0	14.606154761927234	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	4490	0.0	72.69488	1
CGAGGGA	760	0.0	71.57895	4
TACGCGG	45	1.1823431E-10	71.111115	2
TCGCACG	30	3.7962527E-6	66.666664	1
TAGGGTA	1000	0.0	66.4	5
TTGCGGG	1195	0.0	65.6067	3
TTTAGCG	160	0.0	65.0	1
TATTGCG	235	0.0	64.680855	1
AGGGATT	2795	0.0	64.40071	6
TTAGCGG	390	0.0	63.58974	2
CGTAAGG	330	0.0	63.0303	2
TAGGGAT	2400	0.0	62.0	5
TAGCGCG	130	0.0	61.53846	1
AATGCGG	340	0.0	61.17647	2
TACGGGA	715	0.0	60.979023	4
TGGTACG	125	0.0	60.8	1
TAGCGGG	1360	0.0	60.000004	3
CCGATCC	935	0.0	59.89305	80
GCGCGAC	115	0.0	59.130436	9
TCGATGG	230	0.0	59.130436	2
>>END_MODULE