FastQCFastQC Report
Thu 26 May 2016
SRR1547814_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547814_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1807192
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137910.7631175879485964No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64700.073.693971
ACGGGAT10300.067.184465
GGGCGAT10900.065.688077
TAAGGGA28250.065.274344
CGAACGG1050.064.76192
TACGGGA7500.064.533334
TAGCGGG11150.062.780273
TAGGGAC22050.062.4036255
GGCGATA2250.062.222238
ATAGCGG3850.061.2987022
CGGGATT11700.060.512826
AGGGATT34750.060.201446
GCGGGAT15600.060.05
GTGCGGG10800.059.6296273
GTAGGGA30150.059.436154
CGGGATC6300.059.047626
TATGGGA26050.058.9635284
AAGGGAT32500.058.9538465
TTGCGGG15500.058.3225823
TAGGGAG24850.058.2696155
Produced by FastQC (version 0.11.5)