##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547814_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1807192
Sequences flagged as poor quality	0
Sequence length	86
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14029333905861	33.0	31.0	34.0	31.0	34.0
2	32.58815720742456	34.0	31.0	34.0	31.0	34.0
3	32.70102678630715	34.0	31.0	34.0	31.0	34.0
4	36.20962133519847	37.0	35.0	37.0	35.0	37.0
5	36.1898248774895	37.0	35.0	37.0	35.0	37.0
6	36.25246459701017	37.0	37.0	37.0	35.0	37.0
7	36.395490905227554	37.0	37.0	37.0	35.0	37.0
8	36.38895092497089	37.0	37.0	37.0	35.0	37.0
9	38.18998313405549	39.0	39.0	39.0	37.0	39.0
10-11	37.860056928096185	39.0	38.0	39.0	35.0	39.0
12-13	37.19055999583885	39.0	36.0	39.0	34.5	39.0
14-15	38.357671459369016	40.0	37.0	41.0	34.0	41.0
16-17	38.53563456456204	40.0	37.0	41.0	35.0	41.0
18-19	38.409223259067105	40.0	36.0	41.0	35.0	41.0
20-21	38.21244947963471	40.0	35.0	41.0	34.0	41.0
22-23	38.03772676063196	40.0	35.0	41.0	34.0	41.0
24-25	38.01598225313083	40.0	35.0	41.0	34.0	41.0
26-27	37.99182737639387	40.0	35.0	41.0	34.0	41.0
28-29	37.927559993625465	40.0	35.0	41.0	34.0	41.0
30-31	37.769458364136185	40.0	35.5	41.0	34.0	41.0
32-33	37.46155140128995	40.0	35.0	41.0	33.0	41.0
34-35	37.13816987901673	40.0	35.0	41.0	33.0	41.0
36-37	36.95601325149735	40.0	35.0	41.0	32.0	41.0
38-39	36.854760313237335	40.0	35.0	41.0	32.0	41.0
40-41	36.72654648758959	40.0	35.0	41.0	31.5	41.0
42-43	36.67579648426952	39.0	35.0	41.0	32.0	41.0
44-45	36.55378426863332	39.0	35.0	41.0	31.0	41.0
46-47	36.429039913855306	39.0	35.0	41.0	31.0	41.0
48-49	36.318993222634894	39.0	35.0	41.0	31.0	41.0
50-51	36.091231866896266	38.0	35.0	40.5	30.5	41.0
52-53	35.89268157450896	38.0	35.0	40.0	30.0	41.0
54-55	35.72892005940708	38.0	35.0	40.0	30.0	41.0
56-57	35.45196857887817	37.0	35.0	40.0	29.5	41.0
58-59	35.225003762743526	37.0	35.0	40.0	29.0	41.0
60-61	34.88988303401078	36.0	34.5	39.5	28.5	41.0
62-63	34.53317743770446	35.5	34.0	39.0	27.5	41.0
64-65	34.22600725324149	35.0	34.0	39.0	27.0	40.0
66-67	33.93972970221205	35.0	34.0	38.0	26.5	40.0
68-69	33.491413474605906	35.0	34.0	37.5	25.5	40.0
70-71	33.25086432432193	35.0	33.5	37.0	26.0	39.0
72-73	32.98052558886936	35.0	33.0	36.5	25.0	39.0
74-75	32.690129493711794	35.0	33.0	36.0	25.0	38.5
76-77	30.78397259394685	33.5	30.0	34.5	21.5	36.0
78-79	31.83755378509865	34.5	32.0	35.0	23.5	37.0
80-81	32.01047536731017	35.0	33.0	35.0	24.0	37.0
82-83	31.84857364353096	35.0	33.0	35.0	23.5	36.0
84-85	31.64965980371759	35.0	33.0	35.0	23.0	36.0
86	31.22207601627276	34.0	32.0	35.0	20.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	8.0
10	9.0
11	19.0
12	47.0
13	67.0
14	225.0
15	595.0
16	1491.0
17	3573.0
18	7719.0
19	12309.0
20	15022.0
21	15314.0
22	13434.0
23	11606.0
24	10915.0
25	11380.0
26	12780.0
27	15426.0
28	18446.0
29	23062.0
30	28963.0
31	37807.0
32	49071.0
33	68709.0
34	185253.0
35	170687.0
36	160569.0
37	294817.0
38	435899.0
39	201538.0
40	430.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.42455699228417	23.619460466845805	29.68727174533752	14.268710795532517
2	31.98315397589188	25.287185866249963	29.52093634765979	13.208723810198364
3	29.484747608444483	25.63988773744018	31.575615651242366	13.299749002872966
4	26.59385389045547	26.888288571441223	32.65120695532074	13.866650582782572
5	24.005750357460634	31.623645965674925	29.67792022098371	14.692683455880726
6	22.13710552060877	40.709011549409254	26.397693216880107	10.756189713101874
7	87.19040367597908	3.821287389497076	7.157955546505297	1.8303533880185394
8	88.3455106042966	3.024249775342078	6.779910490971629	1.8503291293896829
9	83.93706922120062	5.614788024736718	7.631673889658652	2.816468864404004
10-11	38.83668697072585	28.917984364693954	20.49884572308864	11.746482941491552
12-13	28.467478829034214	25.498618851787747	28.75209164272529	17.281810676452753
14-15	17.158470157017074	30.454318080203986	34.58871553216261	17.798496230616337
16-17	23.154844642959908	26.33237088256256	31.132248261391155	19.38053621308638
18-19	24.864679569188	27.846460143692536	29.366331856272048	17.922528430847414
20-21	26.60093670180036	27.448522348483174	29.776166561162288	16.174374388554178
22-23	22.252450210049624	23.94410776497461	32.46752420329439	21.33591782168137
24-25	20.965453587665284	27.382646669529304	32.900876055228224	18.75102368757719
26-27	19.301435597324467	32.28121306424552	29.147511719839397	19.26983961859061
28-29	17.928808892469643	28.543757387150897	34.29848627041288	19.228947449966576
30-31	23.110992080531563	27.374982846316275	30.439267106096086	19.074757967056076
32-33	25.762066233139592	27.195726851380485	28.302360789556396	18.73984612592353
34-35	20.45507616235574	28.278815975280992	30.17761809481228	21.088489767550985
36-37	23.26186149562415	29.523288062364156	28.68616062930779	18.52868981270391
38-39	20.908874098601586	30.200858569537715	27.953919672065837	20.936347659794862
40-41	21.640312706120877	26.911971721875705	28.702871637324645	22.74484393467877
42-43	21.199186362046753	26.421736041328202	29.285875546151157	23.093202050473884
44-45	20.432527368425713	29.69476956515965	28.84101412578188	21.031688940632762
46-47	21.590373352693017	29.544647165326097	28.13685540883315	20.728124073147733
48-49	21.734464295990684	26.885355844868723	30.843955705868552	20.536224153272038
50-51	20.236145356995824	29.437270638648243	29.23446982943705	21.09211417491888
52-53	22.169254843978948	26.74713035471605	29.414140832850077	21.66947396845493
54-55	21.708733770401818	27.08021615854873	30.386561029486632	20.82448904156282
56-57	22.011855962177787	26.125558324738048	29.301673535518084	22.56091217756608
58-59	23.72307424999668	27.832543526089093	27.82070195087185	20.62368027304238
60-61	23.879228106366117	28.73936471608993	27.03210284242073	20.349304335123218
62-63	22.681817980601952	26.554151412799527	29.83941385309364	20.924616753504885
64-65	20.780193803425426	29.148148066171164	29.73532972700189	20.33632840340152
66-67	20.36391263352206	28.583764204356815	27.12996184135388	23.922361320767248
68-69	19.119246875816184	31.324175848498665	26.696582322188235	22.85999495349692
70-71	20.245662884740526	29.41239779724567	28.92404348846166	21.417895829552144
72-73	22.57408177991049	29.628866218973965	27.297514597231505	20.499537403884034
74-75	21.184218389634303	30.641182563889174	26.34601082784784	21.828588218628678
76-77	23.709572640870476	28.203256765191526	27.11037344122816	20.97679715270984
78-79	19.913849773571375	28.811742194520562	29.370592609971713	21.90381542193635
80-81	18.637311364813478	28.579863124670762	29.647569267681572	23.13525624283419
82-83	19.635545088734347	28.12761455340661	30.065399802566635	22.17144055529241
84-85	18.599683929543733	27.63447381351843	28.880744270669634	24.8850979862682
86	19.170071580662153	27.94827555677537	30.046281745381787	22.835371117180685
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	886.0
1	1119.5
2	4757.5
3	5249.5
4	2507.5
5	2951.5
6	3304.0
7	3383.0
8	3550.5
9	3704.5
10	3715.0
11	3830.5
12	4092.5
13	4145.0
14	4027.0
15	4084.0
16	4225.5
17	4347.0
18	4340.5
19	4534.5
20	4827.5
21	4883.0
22	5974.0
23	7005.5
24	8271.5
25	10919.5
26	12353.5
27	14468.5
28	19500.5
29	22529.0
30	24043.5
31	28208.0
32	30876.5
33	33679.0
34	41263.5
35	47353.0
36	48642.0
37	49561.0
38	53034.0
39	60439.0
40	66650.5
41	73083.0
42	80361.0
43	82567.0
44	82296.5
45	84398.5
46	89506.5
47	95171.5
48	95062.5
49	90001.0
50	87978.0
51	77926.0
52	65165.0
53	59380.5
54	55679.5
55	52953.5
56	47428.5
57	44004.0
58	39086.0
59	31744.0
60	25493.0
61	19204.5
62	14773.0
63	11261.5
64	9720.0
65	8344.0
66	6004.0
67	4809.5
68	4237.0
69	3332.0
70	2400.5
71	2032.0
72	1728.5
73	1036.0
74	538.5
75	353.5
76	264.5
77	220.0
78	137.0
79	86.0
80	87.5
81	111.0
82	92.0
83	40.0
84	31.0
85	28.5
86	24.0
87	17.0
88	13.5
89	13.5
90	10.5
91	12.0
92	10.0
93	7.0
94	7.0
95	4.5
96	1.5
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1807192.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.80118934373334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.0196174826429	13.444247445389982
2	7.1396885155062275	2.399105172085971
3	2.2765202831220073	1.1474474496474683
4	1.1653938962723338	0.7832001404521045
5	0.7337764585221288	0.616415860780219
6	0.5299286594712614	0.5342059047868472
7	0.4085947737597411	0.48054147111581097
8	0.3315104953155333	0.445581648098488
9	0.27393974120408454	0.4142262114668822
>10	3.7492658630065976	15.769425622105517
>50	1.738690348837517	21.238705861403858
>100	1.6267630499288321	41.239453554159496
>500	0.005314048346031017	0.5826230206510934
>1k	6.642560432538772E-4	0.13526273365178149
>5k	0.0	0.0
>10k+	3.321280216269386E-4	0.7695579042045043
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13791	0.7631175879485964	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1066892726395425E-4	0.0	0.0	0.02019707922567165	0.0
2	1.1066892726395425E-4	0.0	0.0	0.06950008632176327	0.0
3	1.1066892726395425E-4	0.0	0.0	0.1042501294826449	0.0
4	1.1066892726395425E-4	0.0	0.0	0.18000301019482157	0.0
5	1.1066892726395425E-4	0.0	0.0	0.3541959017082856	0.0
6	1.1066892726395425E-4	0.0	0.0	0.4949114427244034	0.0
7	1.1066892726395425E-4	0.0	0.0	0.5910274060531476	0.0
8	1.1066892726395425E-4	0.0	0.0	0.7689277066299541	0.0
9	1.1066892726395425E-4	0.0	0.0	0.8372657692154458	0.0
10	1.1066892726395425E-4	0.0	0.0	0.9897122165215428	0.0
11	1.1066892726395425E-4	0.0	0.0	1.1746953284432424	0.0
12	1.1066892726395425E-4	0.0	0.0	1.342248084320869	0.0
13	1.1066892726395425E-4	0.0	0.0	1.3945391524530875	0.0
14	1.1066892726395425E-4	0.0	0.0	1.4184436407421015	0.0
15	1.1066892726395425E-4	0.0	0.0	1.4569564274299576	0.0
16	1.1066892726395425E-4	0.0	0.0	1.5294445747878476	0.0
17	1.1066892726395425E-4	0.0	0.0	1.6294892850344622	0.0
18	1.1066892726395425E-4	0.0	0.0	1.7424822597709595	0.0
19	1.1066892726395425E-4	0.0	0.0	1.823436580064542	0.0
20	1.1066892726395425E-4	0.0	0.0	1.9166198168207915	0.0
21	1.1066892726395425E-4	0.0	0.0	2.022695983603292	0.0
22	1.1066892726395425E-4	0.0	5.5334463631977125E-5	2.1426057662937863	0.0
23	1.1066892726395425E-4	0.0	5.5334463631977125E-5	2.2560414167393392	0.0
24	1.1066892726395425E-4	0.0	5.5334463631977125E-5	2.3378810884510335	0.0
25	1.6600339089593136E-4	0.0	5.5334463631977125E-5	2.4176180505447125	0.0
26	1.6600339089593136E-4	0.0	5.5334463631977125E-5	2.498517036374663	0.0
27	1.6600339089593136E-4	0.0	5.5334463631977125E-5	2.5917556075945445	0.0
28	1.6600339089593136E-4	0.0	5.5334463631977125E-5	2.684994178814426	0.0
29	1.6600339089593136E-4	0.0	5.5334463631977125E-5	2.8005325388779942	0.0
30	1.6600339089593136E-4	0.0	5.5334463631977125E-5	2.927082457204326	0.0
31	1.6600339089593136E-4	0.0	5.5334463631977125E-5	3.0363126884138487	0.0
32	1.6600339089593136E-4	0.0	5.5334463631977125E-5	3.1336460099424963	0.0
33	1.6600339089593136E-4	0.0	5.5334463631977125E-5	3.2345760716072225	0.0
34	1.6600339089593136E-4	0.0	5.5334463631977125E-5	3.3537111718068693	0.0
35	1.6600339089593136E-4	0.0	5.5334463631977125E-5	3.475225653942691	0.0
36	1.6600339089593136E-4	0.0	5.5334463631977125E-5	3.581689161970615	0.0
37	1.6600339089593136E-4	0.0	5.5334463631977125E-5	3.7090137627877944	0.0
38	1.6600339089593136E-4	0.0	5.5334463631977125E-5	3.867104325384353	0.0
39	1.6600339089593136E-4	0.0	5.5334463631977125E-5	4.192747643858539	0.0
40	1.6600339089593136E-4	0.0	5.5334463631977125E-5	4.355154294618392	0.0
41	1.6600339089593136E-4	0.0	5.5334463631977125E-5	4.49979858255238	0.0
42	1.6600339089593136E-4	0.0	5.5334463631977125E-5	4.643280846750097	0.0
43	1.6600339089593136E-4	0.0	5.5334463631977125E-5	4.774589528948778	0.0
44	1.6600339089593136E-4	0.0	5.5334463631977125E-5	4.9010841128114775	0.0
45	1.6600339089593136E-4	0.0	5.5334463631977125E-5	5.02010854408386	0.0
46	1.6600339089593136E-4	0.0	5.5334463631977125E-5	5.157448682818428	0.0
47	1.6600339089593136E-4	0.0	5.5334463631977125E-5	5.315096569705931	0.0
48	1.6600339089593136E-4	0.0	5.5334463631977125E-5	5.456808131067424	0.0
49	1.6600339089593136E-4	0.0	5.5334463631977125E-5	5.599681716165189	0.0
50	1.6600339089593136E-4	0.0	5.5334463631977125E-5	5.748752761189735	0.0
51	1.6600339089593136E-4	0.0	5.5334463631977125E-5	5.9015312152776245	0.0
52	1.6600339089593136E-4	0.0	5.5334463631977125E-5	6.095257172453176	0.0
53	1.6600339089593136E-4	0.0	5.5334463631977125E-5	6.253181731658839	0.0
54	1.6600339089593136E-4	0.0	5.5334463631977125E-5	6.410442277300918	0.0
55	1.6600339089593136E-4	0.0	5.5334463631977125E-5	6.563331400316071	0.0
56	1.6600339089593136E-4	0.0	5.5334463631977125E-5	6.71948525668551	0.0
57	1.6600339089593136E-4	0.0	5.5334463631977125E-5	6.889196056644784	0.0
58	2.213378545279085E-4	0.0	5.5334463631977125E-5	7.0527647311409085	0.0
59	2.213378545279085E-4	0.0	5.5334463631977125E-5	7.216720746882456	0.0
60	2.213378545279085E-4	0.0	5.5334463631977125E-5	7.408731335685417	0.0
61	2.213378545279085E-4	0.0	5.5334463631977125E-5	7.59858388040673	0.0
62	2.766723181598856E-4	0.0	5.5334463631977125E-5	7.836411405096968	0.0
63	2.766723181598856E-4	0.0	5.5334463631977125E-5	8.045133001916787	0.0
64	2.766723181598856E-4	0.0	5.5334463631977125E-5	8.244226402064639	0.0
65	2.766723181598856E-4	0.0	5.5334463631977125E-5	8.43286158858605	0.0
66	2.766723181598856E-4	0.0	5.5334463631977125E-5	8.608991186326632	0.0
67	2.766723181598856E-4	0.0	5.5334463631977125E-5	8.81516739781938	0.0
68	2.766723181598856E-4	0.0	5.5334463631977125E-5	9.054710290882209	0.0
69	3.320067817918627E-4	0.0	5.5334463631977125E-5	9.451790401905276	0.0
70	3.320067817918627E-4	0.0	5.5334463631977125E-5	9.67805302369643	0.0
71	3.320067817918627E-4	0.0	5.5334463631977125E-5	9.886055272489033	0.0
72	3.320067817918627E-4	0.0	5.5334463631977125E-5	10.087030044400374	0.0
73	3.320067817918627E-4	0.0	5.5334463631977125E-5	10.297522344056414	0.0
74	3.320067817918627E-4	0.0	5.5334463631977125E-5	10.525666337611057	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	6470	0.0	73.69397	1
ACGGGAT	1030	0.0	67.18446	5
GGGCGAT	1090	0.0	65.68807	7
TAAGGGA	2825	0.0	65.27434	4
CGAACGG	105	0.0	64.7619	2
TACGGGA	750	0.0	64.53333	4
TAGCGGG	1115	0.0	62.78027	3
TAGGGAC	2205	0.0	62.403625	5
GGCGATA	225	0.0	62.22223	8
ATAGCGG	385	0.0	61.298702	2
CGGGATT	1170	0.0	60.51282	6
AGGGATT	3475	0.0	60.20144	6
GCGGGAT	1560	0.0	60.0	5
GTGCGGG	1080	0.0	59.629627	3
GTAGGGA	3015	0.0	59.43615	4
CGGGATC	630	0.0	59.04762	6
TATGGGA	2605	0.0	58.963528	4
AAGGGAT	3250	0.0	58.953846	5
TTGCGGG	1550	0.0	58.322582	3
TAGGGAG	2485	0.0	58.269615	5
>>END_MODULE