FastQCFastQC Report
Thu 26 May 2016
SRR1547813_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547813_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2044866
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209691.0254461661546528No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACG550.080.01
TACGCGG451.8189894E-1280.02
ACGCTAG404.1836756E-1180.01
TCGCGAG150.001309937780.01
CGTTTTT92350.074.759061
CGCGGAT700.074.285714
CTAACGG1550.072.2580642
CGGGATA9000.071.555566
GTCGAAG3400.070.588241
TATTGCG2050.070.2439041
ACGGGAT13450.069.888475
GGGCGAT16850.069.3175057
CGAGGGT2600.069.2307744
TACGGGA8150.067.7300644
AGTACGG2900.067.586212
CGTAAGG3850.067.532462
CGTTAGG3500.067.428572
TAGGTCG950.067.368421
TAACGGG7600.067.368423
AGGGATT48600.067.24286
Produced by FastQC (version 0.11.5)