FastQCFastQC Report
Thu 26 May 2016
SRR1547812_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547812_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3838084
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308580.8039949099602822No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT156800.072.066321
TACGGGA19750.065.4177254
CGATAGG6850.065.401462
CCGATCC19850.063.2745680
TATAGCG3500.062.857141
CGAGGGA24300.062.386834
AGGGATT79550.062.3507276
TAGGGTA35700.061.960785
TAGGGAT69150.061.8944325
TAGGGAC39650.061.6393475
GTACGGG18450.061.355013
CGAGGGT10700.061.308414
ATAGGGA59250.061.299584
CGTAAGG7050.060.709222
TAGCGGG29200.060.5479473
AGGGATC45350.060.5071686
ACGGGAT24300.060.411525
CGTTAGG5700.060.350882
TAAGGGA51400.060.1556434
CTATGCG1800.060.01
Produced by FastQC (version 0.11.5)