FastQCFastQC Report
Thu 26 May 2016
SRR1547811_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547811_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3774306
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342070.9063123127801508No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT162450.071.825181
GGGCGAT25350.068.481267
TACGGGA20750.066.506034
ATACGCG1050.064.761911
AGGGATT83550.064.296836
GCGCGAC2250.064.09
TAGGGAC49750.063.7587935
CGGGATA13700.063.3576626
GCGGGAT35050.062.995725
TAGGGCG7500.062.9333345
GGCGATA6700.062.0895548
ATAGGGA59300.061.8549774
CGAGGGA31550.061.7432634
CGTTAGG5250.061.7142872
AAGGGAT74400.061.397855
TATAGCG3550.060.845071
AGGGCGA15400.060.7792176
GCGTAAG2250.060.4444431
GAGGGAT78300.060.229885
ACGGGAC13150.060.2281385
Produced by FastQC (version 0.11.5)