FastQCFastQC Report
Thu 26 May 2016
SRR1547810_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547810_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1528190
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90570.5926619072235783No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20180.1320516427931082No Hit
AGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAAAGGAAAGGAAAGGAAAGG15790.10332484835000884No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGG4400.073.636363
CGTTTTT43800.072.420091
TAGTGCG503.6379788E-1272.01
TGTTGCG1400.068.571431
TGTTCGG700.068.571432
TTGCGGG12400.068.064523
TTAGGGA20900.067.942584
CTAACGG1300.067.692312
GACGGGT2550.067.450974
AGGGATT27250.066.6422046
TACGGGA5350.066.542064
GTATGCG559.094947E-1265.4545441
GCGATGA2150.065.116289
AACGGGA5900.065.084754
CTATGCG800.065.01
CTTGCGG2600.064.615392
GTACGGG5850.064.27353
ATGGGAC10900.064.2201845
GCGCGAC503.0013325E-1064.09
CGTTAGG1750.064.02
Produced by FastQC (version 0.11.5)