##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547810_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1528190
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.760273918818996	31.0	31.0	33.0	30.0	34.0
2	32.19287981206525	33.0	31.0	34.0	30.0	34.0
3	32.34707660696641	34.0	31.0	34.0	30.0	34.0
4	35.95093672907165	37.0	35.0	37.0	35.0	37.0
5	35.89998233203986	37.0	35.0	37.0	35.0	37.0
6	35.9476485253797	37.0	35.0	37.0	35.0	37.0
7	36.20939411984112	37.0	35.0	37.0	35.0	37.0
8	36.1555807851118	37.0	35.0	37.0	35.0	37.0
9	38.022255086082225	39.0	39.0	39.0	35.0	39.0
10-11	37.49765147003972	39.0	37.0	39.0	35.0	39.0
12-13	36.547241835112125	39.0	35.0	39.0	33.0	39.0
14-15	37.50003664465806	40.0	35.0	41.0	33.0	41.0
16-17	37.723669504446434	40.0	35.0	41.0	33.0	41.0
18-19	37.63088130402633	39.0	35.5	41.0	33.0	41.0
20-21	37.41764080382675	39.0	35.0	41.0	33.0	41.0
22-23	37.21888933967635	39.0	35.0	41.0	33.0	41.0
24-25	37.13114010692388	39.0	35.0	41.0	33.0	41.0
26-27	37.10006510970494	39.0	35.0	41.0	33.0	41.0
28-29	37.106493956903265	39.0	35.0	41.0	33.0	41.0
30-31	36.94699644677691	39.0	35.0	41.0	32.5	41.0
32-33	36.632715500035985	38.0	35.0	41.0	31.5	41.0
34-35	36.35663104718654	38.0	35.0	41.0	31.0	41.0
36-37	36.166685425241624	38.0	35.0	41.0	30.0	41.0
38-39	36.088381025919546	38.0	35.0	41.0	30.0	41.0
40-41	35.93872980454002	38.0	35.0	40.5	30.0	41.0
42-43	35.89764492635078	38.0	35.0	41.0	30.0	41.0
44-45	35.82009959494566	38.0	35.0	40.0	30.0	41.0
46-47	35.73931939091344	38.0	35.0	40.0	30.0	41.0
48-49	35.64050118113585	37.0	35.0	40.0	29.5	41.0
50-51	35.448699114638885	37.0	35.0	40.0	29.0	41.0
52-53	35.28382956307789	36.5	34.5	40.0	29.0	41.0
54-55	35.08153403699802	36.0	34.0	40.0	28.5	41.0
56-57	34.75874138686943	36.0	34.0	40.0	27.5	41.0
58-59	34.46118283721265	35.0	34.0	39.0	26.0	41.0
60-61	34.16459962439225	35.0	34.0	39.0	26.0	41.0
62-63	33.72908342549029	35.0	33.0	39.0	25.5	40.0
64-65	33.46814990282622	35.0	33.0	38.0	25.0	40.0
66-67	33.22151205020318	35.0	33.0	37.0	25.0	40.0
68-69	32.83203593793965	35.0	33.0	37.0	23.5	39.5
70-71	32.59478893331327	35.0	33.0	36.0	23.5	39.0
72-73	32.37235225986298	35.0	33.0	36.0	23.0	39.0
74-75	32.073541902512126	35.0	32.0	35.5	21.5	37.5
76-77	30.04797407390442	33.0	29.5	34.5	18.5	36.0
78-79	31.111826736204268	34.0	30.5	35.0	19.5	36.0
80-81	31.420209201735386	35.0	32.0	35.0	20.0	36.0
82-83	31.281998311728252	35.0	32.0	35.0	19.5	36.0
84-85	31.07986441476518	34.0	32.0	35.0	19.0	36.0
86	30.662470635195884	34.0	31.0	35.0	18.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	9.0
11	10.0
12	31.0
13	78.0
14	241.0
15	716.0
16	1779.0
17	3840.0
18	7119.0
19	10470.0
20	12508.0
21	12684.0
22	11632.0
23	10868.0
24	11626.0
25	13328.0
26	15892.0
27	19301.0
28	23434.0
29	29647.0
30	36493.0
31	44486.0
32	56282.0
33	75653.0
34	190882.0
35	175933.0
36	129218.0
37	219043.0
38	281643.0
39	132616.0
40	718.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.261505441077354	23.437596110431294	29.273061595743982	14.027836852747368
2	30.23230095734169	25.075677762581876	31.195662842971096	13.496358437105332
3	29.15946315575943	25.071031743435046	32.34879170783738	13.420713392968153
4	25.733972869865656	27.63262421557529	31.989412311296373	14.643990603262683
5	24.69948108546712	31.858080474286577	29.230265870081602	14.212172570164705
6	22.526125678089766	40.76325587786859	25.98629751536131	10.724320928680333
7	87.81453876808511	3.712692793435371	6.860141736302423	1.6126267021770853
8	87.95843448785818	3.3368233007675747	6.608275149032515	2.0964670623417248
9	83.57841629640293	5.950896158200224	7.806882652026253	2.663804893370589
10-11	41.76813746981723	27.060771239178372	19.40782886944686	11.763262421557528
12-13	32.33020763125004	26.282497595194314	25.432505120436595	15.95478965311905
14-15	15.54119579371675	33.401671258155076	34.56144196729464	16.495690980833537
16-17	20.534488512554066	25.736524908551946	33.40919650043516	20.31979007845883
18-19	24.102434906654278	28.043404288733733	29.571617403595102	18.28254340101689
20-21	26.812012904154585	28.654486680321163	28.599748722344735	15.933751693179513
22-23	22.132653662175514	25.495422689587027	30.09288766449198	22.279035983745477
24-25	19.528003716815316	26.99458182555834	35.18894901811947	18.28846543950687
26-27	17.290454720944386	35.75566519869911	28.77145512010941	18.18242496024709
28-29	15.992710330521728	29.651973903768507	35.57119860750299	18.78411715820677
30-31	21.974198234512723	30.016457377682094	29.631426720499416	18.37791766730577
32-33	26.398419044752288	28.59062027627455	27.367702968871672	17.643257710101494
34-35	19.545148181836026	28.765762110732307	30.019990969709266	21.669098737722404
36-37	23.07628632565322	30.168434553295075	29.380345375902213	17.37493374514949
38-39	19.583723228132627	31.767581256257404	28.010849436261196	20.637846079348773
40-41	20.56203744298811	27.419136363933806	28.677651339165944	23.341174853912143
42-43	20.095930479848708	26.484599428081584	28.98199831172825	24.43747178034145
44-45	18.863688415707472	32.33537714551201	27.453719759977492	21.347214678803027
46-47	20.456553177288164	31.804749409432077	26.844011543067285	20.894685870212474
48-49	20.909703636327944	27.69760304674157	31.178845562397346	20.21384775453314
50-51	18.885544336764408	31.819014651319534	28.00374953376216	21.291691478153897
52-53	21.520818746360074	28.069480889156452	29.414208966162587	20.99549139832089
54-55	22.968511768824555	27.254824334670424	30.139053389958054	19.63761050654696
56-57	22.699500716533937	26.207179735504095	28.604231149268088	22.48908839869388
58-59	23.934687440697818	27.727834889640686	27.57723188870494	20.760245780956556
60-61	24.61909186684902	29.751012635863344	26.427505742087043	19.202389755200596
62-63	23.24710932541111	26.470203312415343	30.794861895444935	19.487825466728616
64-65	19.321386738559994	31.149955175730764	29.891407482053932	19.637250603655303
66-67	18.375398347064174	29.70932933732062	25.068479704748754	26.846792610866448
68-69	17.648394505918766	33.063722442889954	24.714531570027287	24.57335148116399
70-71	18.818046185356533	30.446443177877097	28.929027149765407	21.806483487000964
72-73	21.988986971515324	31.139223525870474	26.2448713837939	20.626918118820303
74-75	21.59378087803218	32.61184800319332	23.879458706050947	21.91491241272355
76-77	25.804971894856006	29.682205746667627	24.798748846674826	19.714073511801544
78-79	18.94106753741354	30.270123479410284	29.599657110699585	21.18915187247659
80-81	16.418475451350943	29.166693932037248	30.52182647445671	23.8930041421551
82-83	18.39273912275306	27.57746091781781	31.448510983581883	22.581288975847244
84-85	16.466669720388172	28.015004678737593	29.04079335684699	26.477532244027245
86	18.113781663274857	27.62588421596791	30.609610061576113	23.650724059181123
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1450.0
1	1660.0
2	3987.0
3	3963.5
4	2079.5
5	2875.5
6	3725.5
7	4036.0
8	4331.5
9	4459.0
10	4164.0
11	3738.0
12	3540.5
13	3570.0
14	3498.0
15	3490.5
16	3555.5
17	3635.5
18	3983.5
19	4391.5
20	4742.5
21	5026.0
22	5393.0
23	5763.5
24	6298.5
25	7516.0
26	8858.0
27	11944.5
28	15506.0
29	16637.0
30	18397.5
31	20983.5
32	24575.0
33	28351.5
34	33488.5
35	37701.0
36	37787.0
37	39749.5
38	45440.0
39	50279.5
40	57460.5
41	67012.5
42	75163.0
43	79831.0
44	80668.0
45	83499.5
46	88052.0
47	92244.0
48	89814.0
49	79857.5
50	73965.0
51	64818.5
52	50744.5
53	43188.5
54	39465.0
55	37625.5
56	34994.5
57	33108.0
58	29432.0
59	21525.0
60	15495.5
61	13275.0
62	11185.0
63	8160.5
64	6804.0
65	5524.5
66	3605.5
67	2680.0
68	1948.5
69	1224.5
70	792.0
71	638.0
72	541.5
73	424.5
74	381.5
75	226.0
76	109.5
77	82.0
78	35.5
79	33.0
80	25.0
81	12.0
82	6.5
83	5.5
84	6.0
85	7.0
86	7.0
87	4.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1528190.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.01773486969811
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.5827395770365	14.519184367729727
2	8.484146679418918	3.0573021093079933
3	2.456072889118173	1.3275861041035406
4	1.1393449497360328	0.8211366091789343
5	0.6927601039785505	0.6240983940895009
6	0.4906478179709272	0.5304217379157641
7	0.3887346986754235	0.49028831147700364
8	0.2985083821699102	0.43027559090559686
9	0.2558846244871394	0.41494151891173847
>10	2.423671449705829	10.248146789262297
>50	1.1111224987915775	14.559168901014566
>100	1.6679625744287028	51.17717178556242
>500	0.006576851334001457	0.8026397535418262
>1k	0.0014615225186669905	0.4013857100840316
>5k	3.653806296667476E-4	0.5962523169150524
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9057	0.5926619072235783	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2018	0.1320516427931082	No Hit
AGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAAAGGAAAGGAAAGGAAAGG	1579	0.10332484835000884	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.543688939202586E-5	0.0	0.0	0.05261125907118879	0.0
2	6.543688939202586E-5	0.0	0.0	0.1860370765415295	0.0
3	6.543688939202586E-5	0.0	0.0	0.2674405669452097	0.0
4	6.543688939202586E-5	0.0	0.0	0.4008009475261584	0.0
5	6.543688939202586E-5	0.0	0.0	0.6724294753924578	0.0
6	6.543688939202586E-5	0.0	0.0	0.9001498504767077	0.0
7	6.543688939202586E-5	0.0	0.0	1.0468593564936297	0.0
8	6.543688939202586E-5	0.0	0.0	1.343353902328899	0.0
9	6.543688939202586E-5	0.0	0.0	1.4356199163716554	0.0
10	6.543688939202586E-5	0.0	0.0	1.6009789358653046	0.0
11	6.543688939202586E-5	0.0	0.0	1.9159921213985172	0.0
12	6.543688939202586E-5	0.0	0.0	2.1242777403333357	0.0
13	6.543688939202586E-5	0.0	0.0	2.203783560944647	0.0
14	6.543688939202586E-5	0.0	0.0	2.2383996754330284	0.0
15	6.543688939202586E-5	0.0	0.0	2.294871710978347	0.0
16	6.543688939202586E-5	0.0	0.0	2.411741995432505	0.0
17	6.543688939202586E-5	0.0	0.0	2.5524313076253606	0.0
18	6.543688939202586E-5	0.0	0.0	2.712359065299472	0.0
19	6.543688939202586E-5	0.0	0.0	2.8220312919205073	0.0
20	6.543688939202586E-5	0.0	0.0	2.9419771101760905	0.0
21	6.543688939202586E-5	0.0	0.0	3.0977823438185044	0.0
22	6.543688939202586E-5	0.0	0.0	3.2552234996957186	0.0
23	6.543688939202586E-5	0.0	0.0	3.4228073734286966	0.0
24	6.543688939202586E-5	0.0	0.0	3.550605618411323	0.0
25	6.543688939202586E-5	0.0	0.0	3.6625026992716876	0.0
26	6.543688939202586E-5	0.0	0.0	3.7827757019742307	0.0
27	6.543688939202586E-5	0.0	0.0	3.9029178308979904	0.0
28	6.543688939202586E-5	0.0	0.0	4.037717823045564	0.0
29	6.543688939202586E-5	0.0	0.0	4.194046551803114	0.0
30	6.543688939202586E-5	0.0	0.0	4.371773143391856	0.0
31	6.543688939202586E-5	0.0	0.0	4.535234493093136	0.0
32	6.543688939202586E-5	0.0	0.0	4.6799154555389055	0.0
33	6.543688939202586E-5	0.0	0.0	4.832056223375366	0.0
34	6.543688939202586E-5	0.0	0.0	4.998135048652327	0.0
35	6.543688939202586E-5	0.0	0.0	5.176777756692558	0.0
36	6.543688939202586E-5	0.0	0.0	5.33997735883627	0.0
37	6.543688939202586E-5	0.0	0.0	5.510702203260066	0.0
38	6.543688939202586E-5	0.0	0.0	5.705704133648303	0.0
39	6.543688939202586E-5	0.0	0.0	6.006321203515269	0.0
40	6.543688939202586E-5	0.0	0.0	6.213298084662248	0.0
41	6.543688939202586E-5	0.0	0.0	6.423088752053083	0.0
42	6.543688939202586E-5	0.0	0.0	6.627513594513771	0.0
43	6.543688939202586E-5	0.0	0.0	6.8037351376464965	0.0
44	6.543688939202586E-5	0.0	0.0	6.996970272021149	0.0
45	6.543688939202586E-5	0.0	0.0	7.196421910888044	0.0
46	6.543688939202586E-5	0.0	0.0	7.410727723646929	0.0
47	6.543688939202586E-5	0.0	0.0	7.637270234722123	0.0
48	6.543688939202586E-5	0.0	0.0	7.85262303771128	0.0
49	6.543688939202586E-5	0.0	0.0	8.0674523455853	0.0
50	6.543688939202586E-5	0.0	0.0	8.305642622972274	0.0
51	6.543688939202586E-5	0.0	0.0	8.545599696372832	0.0
52	6.543688939202586E-5	0.0	0.0	8.82455715585104	0.0
53	1.3087377878405172E-4	0.0	0.0	9.061765879897132	0.0
54	1.963106681760776E-4	0.0	0.0	9.31906372898658	0.0
55	1.963106681760776E-4	0.0	0.0	9.561834588630996	0.0
56	1.963106681760776E-4	0.0	0.0	9.804081953160274	0.0
57	1.963106681760776E-4	0.0	0.0	10.054116307527206	0.0
58	1.963106681760776E-4	0.0	0.0	10.284388721297745	0.0
59	1.963106681760776E-4	0.0	0.0	10.534161328107107	0.0
60	1.963106681760776E-4	0.0	0.0	10.815474515603427	0.0
61	1.963106681760776E-4	0.0	0.0	11.084878189230396	0.0
62	1.963106681760776E-4	0.0	0.0	11.390206715133589	0.0
63	2.6174755756810345E-4	0.0	0.0	11.726421452829818	0.0
64	3.271844469601293E-4	0.0	0.0	12.025860658687728	0.0
65	3.926213363521552E-4	0.0	0.0	12.323794816089622	0.0
66	3.926213363521552E-4	0.0	0.0	12.58665480077739	0.0
67	3.926213363521552E-4	0.0	0.0	12.858675949980041	0.0
68	3.926213363521552E-4	0.0	0.0	13.197050105026207	0.0
69	3.926213363521552E-4	0.0	0.0	13.655959010332484	0.0
70	3.926213363521552E-4	0.0	0.0	13.958277439323645	0.0
71	3.926213363521552E-4	0.0	0.0	14.250714898016607	0.0
72	3.926213363521552E-4	0.0	0.0	14.53889895889909	0.0
73	3.926213363521552E-4	0.0	0.0	14.833037776716246	0.0
74	3.926213363521552E-4	0.0	0.0	15.166307854389833	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGGG	440	0.0	73.63636	3
CGTTTTT	4380	0.0	72.42009	1
TAGTGCG	50	3.6379788E-12	72.0	1
TGTTGCG	140	0.0	68.57143	1
TGTTCGG	70	0.0	68.57143	2
TTGCGGG	1240	0.0	68.06452	3
TTAGGGA	2090	0.0	67.94258	4
CTAACGG	130	0.0	67.69231	2
GACGGGT	255	0.0	67.45097	4
AGGGATT	2725	0.0	66.642204	6
TACGGGA	535	0.0	66.54206	4
GTATGCG	55	9.094947E-12	65.454544	1
GCGATGA	215	0.0	65.11628	9
AACGGGA	590	0.0	65.08475	4
CTATGCG	80	0.0	65.0	1
CTTGCGG	260	0.0	64.61539	2
GTACGGG	585	0.0	64.2735	3
ATGGGAC	1090	0.0	64.220184	5
GCGCGAC	50	3.0013325E-10	64.0	9
CGTTAGG	175	0.0	64.0	2
>>END_MODULE