FastQCFastQC Report
Thu 26 May 2016
SRR1547809_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547809_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1297654
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130111.0026555614979031No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAG27990.2156969423282323No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT23610.18194372305714773No Hit
CTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC22030.16976790423333185Illumina PCR Primer Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG21070.16236993836569688Illumina PCR Primer Index 10 (95% over 22bp)
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT15620.12037106963797746No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA14280.11004474228107029No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGC13990.10780994009188889No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAA12990.1001037256464358No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCG150.001309829380.01
TAATACG600.080.01
TTGCGCA500.080.01
CGAACGG404.1836756E-1179.999992
TACTACG404.1836756E-1179.999991
GGTACGT1450.077.241388
GCGTAAG1900.075.789481
TACGGGA2800.075.714294
GCGCGAC900.075.555559
CGTAAGG2650.072.4528352
CGTTTTT46050.071.921821
ACGGGAT2600.070.769235
GTAGGGT14900.070.0671164
TATTACG403.7143764E-969.999991
TAGGGAC13150.069.9619755
TACGGGT750.069.3333364
AGGGTAA15100.068.8741766
TAGGGTA17100.068.771935
CTAGTCG351.1815973E-768.571431
GCGATAT351.1815973E-768.571439
Produced by FastQC (version 0.11.5)