##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547809_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1297654
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.655915213146184	31.0	31.0	33.0	30.0	34.0
2	32.08237172620745	33.0	31.0	34.0	30.0	34.0
3	32.230154571249344	33.0	31.0	34.0	30.0	34.0
4	35.8556217605001	37.0	35.0	37.0	35.0	37.0
5	35.78021876401568	37.0	35.0	37.0	35.0	37.0
6	35.85824880900456	37.0	35.0	37.0	35.0	37.0
7	36.186565910481534	37.0	35.0	37.0	35.0	37.0
8	36.132616244391805	37.0	35.0	37.0	35.0	37.0
9	37.975155935249305	39.0	39.0	39.0	35.0	39.0
10-11	37.37026780636441	39.0	37.0	39.0	34.5	39.0
12-13	36.630721671570385	39.0	35.0	39.0	33.0	39.0
14-15	37.58435646173787	40.0	35.0	41.0	33.0	41.0
16-17	37.78149529843857	40.0	35.0	41.0	33.0	41.0
18-19	37.712973566143205	39.0	36.0	41.0	33.0	41.0
20-21	37.49819751644121	39.0	35.0	41.0	33.0	41.0
22-23	37.316727725572456	39.0	35.0	41.0	32.5	41.0
24-25	37.21365017177152	39.0	35.0	41.0	32.5	41.0
26-27	37.18318635013648	39.0	35.0	41.0	33.0	41.0
28-29	37.16726068736351	39.0	35.0	41.0	33.0	41.0
30-31	36.96992071846579	39.0	35.0	41.0	32.0	41.0
32-33	36.68399781451758	39.0	35.0	41.0	31.0	41.0
34-35	36.412903208405325	38.0	35.0	41.0	31.0	41.0
36-37	36.218742052966356	38.0	35.0	41.0	30.0	41.0
38-39	36.131505778890215	38.0	35.0	41.0	30.0	41.0
40-41	35.96290074241669	38.0	35.0	41.0	30.0	41.0
42-43	35.9134565146025	38.0	35.0	41.0	30.0	41.0
44-45	35.76958804118817	38.0	35.0	40.0	29.5	41.0
46-47	35.68274748122381	38.0	35.0	40.0	29.0	41.0
48-49	35.559958586803575	37.5	35.0	40.0	29.0	41.0
50-51	35.34920595166354	37.0	34.5	40.0	28.0	41.0
52-53	35.17235064200472	37.0	34.0	40.0	28.0	41.0
54-55	34.952952790189066	36.5	34.0	40.0	27.0	41.0
56-57	34.583602023343666	36.0	34.0	40.0	26.0	41.0
58-59	34.217713273337885	35.5	34.0	39.0	25.5	41.0
60-61	33.83712376334523	35.0	33.0	39.0	24.5	40.5
62-63	33.32910275003969	35.0	32.5	38.5	22.5	40.0
64-65	32.81450872112289	35.0	32.0	37.5	21.5	40.0
66-67	32.398044856333044	35.0	32.0	37.0	19.5	40.0
68-69	31.856020942408378	35.0	31.5	36.5	17.5	39.0
70-71	31.489395478301613	35.0	31.0	36.0	16.0	39.0
72-73	31.15185904717282	35.0	31.0	35.5	13.0	38.5
74-75	30.81894095036119	35.0	31.0	35.0	11.5	37.0
76-77	28.828860389595377	32.5	27.5	34.5	10.0	35.5
78-79	29.84751212572843	34.0	29.0	35.0	10.0	36.0
80-81	29.986696762002815	34.0	30.0	35.0	10.0	36.0
82-83	29.73915273254658	34.0	29.0	35.0	10.0	36.0
84-85	29.50232612083036	34.0	29.0	35.0	10.0	35.0
86	29.109225571685517	34.0	29.0	35.0	8.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	7.0
11	13.0
12	14.0
13	53.0
14	209.0
15	548.0
16	1401.0
17	3347.0
18	6085.0
19	8559.0
20	9895.0
21	10530.0
22	10250.0
23	10653.0
24	12179.0
25	14801.0
26	17818.0
27	21939.0
28	26791.0
29	33202.0
30	40830.0
31	48662.0
32	55813.0
33	67894.0
34	143499.0
35	134900.0
36	113913.0
37	180228.0
38	225616.0
39	97695.0
40	307.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.329804400864944	24.583825888873307	28.939147107010033	14.147222603251713
2	29.8935617660794	25.643045064400834	31.315435393409953	13.147957776109811
3	30.000446960437838	24.04169370263568	32.687295688989515	13.270563647936969
4	28.493342601340572	26.157974313646008	31.830749953377403	13.517933131636015
5	22.935235432557523	32.4141103868982	30.05462164798937	14.596032532554904
6	22.017502354248514	41.26192344030073	25.960232850975686	10.760341354475075
7	87.10696379774578	3.6565987543674967	7.153987118291933	2.0824503295947916
8	86.6650894614435	3.3487354872716457	7.393033890389889	2.593141160894969
9	81.35735719999322	5.599412478210679	9.616045571469744	3.4271847503263584
10-11	38.60451245093068	24.57927922235049	21.925181905191984	14.891026421526849
12-13	29.285772632766516	26.42842390960919	27.177583546923913	17.108219910700388
14-15	16.13742954593443	32.19629423559747	34.702817546125544	16.96345867234255
16-17	21.298820795065556	27.1981976705655	31.507397195246188	19.995584339122754
18-19	23.49570840917533	29.166711619584262	29.033663827183513	18.30391614405689
20-21	24.362888720722164	30.09022435872736	28.380061248992412	17.16682567155806
22-23	20.17224930528477	26.94212016454309	30.95370568734038	21.93192484283176
24-25	19.318131027222975	29.56431375389742	32.93139003154924	18.186165187330367
26-27	17.22897629106064	34.22927066845246	28.98769625801639	19.554056782470518
28-29	16.500469308459728	29.978869559990567	34.4294395886731	19.091221542876607
30-31	22.264717713658648	29.773229227513653	29.61710132284877	18.344951735978928
32-33	24.43521154329274	28.816926545905147	28.350700571955233	18.397161338846875
34-35	18.55583229427875	28.295485545453563	30.87132625491849	22.277355905349193
36-37	21.723394679937797	29.20031071456644	29.819697700619734	19.25659690487603
38-39	18.559454215068115	31.357280137848765	28.67066259573045	21.412603051352672
40-41	19.87660038808496	26.92401056059628	29.33120076692246	23.868188284396304
42-43	20.387445343674045	26.978840276375678	28.76279038942584	23.87092399052444
44-45	19.81992118083865	30.627231912358766	28.02330205124016	21.52954485556242
46-47	20.49741302381066	29.99197012454784	28.320338087040152	21.19027876460135
48-49	20.85968216489141	27.861201830380054	30.573981970540682	20.70513403418785
50-51	18.4022859714531	30.29104830717587	29.450685621899215	21.855980099471818
52-53	21.24957808475911	26.447805038939503	30.41477158009762	21.887845296203764
54-55	20.68286307444049	27.275259815020032	30.501967396547926	21.539909713991555
56-57	21.026714362996607	26.38338108617551	30.3655674008634	22.224337149964473
58-59	21.68328383374921	28.491994013812622	29.40911059496599	20.415611557472175
60-61	22.602365499586178	30.429798698266257	27.250638459866806	19.717197342280762
62-63	22.232736923710018	27.55091110573389	29.955250012715258	20.26110195784084
64-65	19.856833948032374	31.50481561340696	29.31644336625942	19.321907072301244
66-67	17.27898962281163	31.499999229378556	27.11828422676615	24.10272692104367
68-69	17.113151887945477	33.90075474664279	26.14206098081615	22.844032384595586
70-71	18.314011284980435	31.279062061227414	28.447644749679036	21.959281904113116
72-73	21.14311673219518	31.25995064940269	27.56747946679161	20.02945315161052
74-75	19.50966898726471	33.46284911077991	24.886988365157432	22.140493536797944
76-77	22.89119441700176	30.3888786995609	26.618459157834064	20.101467725603282
78-79	17.651392435888148	31.508899907063054	28.80058937128079	22.03911828576801
80-81	15.943155879764559	30.313666046573278	29.907047641358947	23.836130432303218
82-83	18.26053015672899	29.18416619530322	30.693736543023025	21.861567104944772
84-85	16.087840055977942	28.524128928050157	29.64630787559704	25.741723140374862
86	17.203199003740597	28.93668111838749	30.147096221334806	23.713023656537104
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	822.0
1	1306.5
2	5317.0
3	5362.5
4	2206.0
5	3241.5
6	4789.0
7	5625.0
8	5977.0
9	6016.0
10	5349.5
11	4713.0
12	4237.0
13	3931.5
14	3819.0
15	3820.5
16	3927.5
17	3930.0
18	4158.5
19	4214.0
20	4079.5
21	4221.0
22	4414.5
23	4929.0
24	5578.5
25	6521.5
26	6892.5
27	7585.0
28	10220.5
29	11920.0
30	14312.0
31	17019.5
32	19054.5
33	21999.5
34	25691.5
35	28477.5
36	28797.0
37	31437.0
38	38176.0
39	43227.5
40	48088.0
41	58432.5
42	67424.0
43	69979.0
44	70986.0
45	74337.0
46	77086.5
47	77838.5
48	75104.5
49	70098.5
50	68173.0
51	58123.0
52	43663.0
53	37457.0
54	34321.0
55	31087.5
56	28110.5
57	27027.0
58	23983.5
59	18063.0
60	13027.5
61	9380.0
62	6162.0
63	3573.5
64	2714.0
65	2354.5
66	1662.5
67	1203.0
68	1040.5
69	883.0
70	596.0
71	431.0
72	348.5
73	217.0
74	173.5
75	145.5
76	70.0
77	26.0
78	15.5
79	7.0
80	12.5
81	14.0
82	6.0
83	2.0
84	2.0
85	3.0
86	4.0
87	3.5
88	1.5
89	0.5
90	1.5
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1297654.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.78123779564754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.53242506546655	15.90438524363037
2	10.204438896932476	4.241217425766181
3	3.6110136086679803	2.2512399745514595
4	1.7853844443002223	1.4840999477461183
5	1.0201832899845438	1.060033577215743
6	0.7516109818046057	0.9371643925620976
7	0.5385526777993791	0.7834253883982154
8	0.4626817437312184	0.7692079472146643
9	0.35402563412733684	0.6621381799699589
>10	2.9151173657010316	12.613430608506546
>50	0.7246081473721167	10.95945007916406
>100	1.0850485115254538	44.21115810176692
>500	0.00820029792289934	1.142847127748036
>1k	0.006336593849513127	1.9723679282803472
>5k	0.0	0.0
>10k+	3.7274081467724275E-4	1.007834077479307
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13011	1.0026555614979031	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAG	2799	0.2156969423282323	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2361	0.18194372305714773	No Hit
CTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC	2203	0.16976790423333185	Illumina PCR Primer Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG	2107	0.16236993836569688	Illumina PCR Primer Index 10 (95% over 22bp)
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1562	0.12037106963797746	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1428	0.11004474228107029	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGC	1399	0.10780994009188889	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAA	1299	0.1001037256464358	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18101897732369338	0.0
2	0.0	0.0	0.0	0.6005452917341603	0.0
3	0.0	0.0	0.0	0.8473753404220231	0.0
4	0.0	0.0	0.0	1.1950797362008672	0.0
5	0.0	0.0	0.0	1.9804971124814472	0.0
6	0.0	0.0	0.0	2.618263419987146	0.0
7	0.0	0.0	0.0	3.033474254308159	0.0
8	0.0	0.0	0.0	3.8171192012662853	0.0
9	0.0	0.0	0.0	4.06942066221042	0.0
10	0.0	0.0	0.0	4.570632849742689	0.0
11	0.0	0.0	0.0	5.409146043552441	0.0
12	0.0	0.0	0.0	5.971083200914882	0.0
13	0.0	0.0	0.0	6.188090199698841	0.0
14	0.0	0.0	0.0	6.275555733654734	0.0
15	0.0	0.0	0.0	6.430065333286069	0.0
16	0.0	0.0	0.0	6.7645150402187335	0.0
17	0.0	0.0	0.0	7.141965423757026	0.0
18	7.706214445453102E-5	0.0	0.0	7.604492414773121	0.0
19	7.706214445453102E-5	0.0	0.0	7.887002236343432	0.0
20	7.706214445453102E-5	0.0	0.0	8.191860079805556	0.0
21	7.706214445453102E-5	0.0	0.0	8.573548881288849	0.0
22	7.706214445453102E-5	0.0	0.0	8.948841524782415	0.0
23	7.706214445453102E-5	0.0	0.0	9.308875863673984	0.0
24	7.706214445453102E-5	0.0	0.0	9.581752917187478	0.0
25	7.706214445453102E-5	0.0	0.0	9.852626354945155	0.0
26	7.706214445453102E-5	0.0	0.0	10.080807364675021	0.0
27	7.706214445453102E-5	0.0	0.0	10.346980011620971	0.0
28	7.706214445453102E-5	0.0	0.0	10.608991302766377	0.0
29	7.706214445453102E-5	0.0	0.0	10.921015925662774	0.0
30	7.706214445453102E-5	0.0	0.0	11.287523484688522	0.0
31	7.706214445453102E-5	0.0	0.0	11.58698697803883	0.0
32	1.5412428890906204E-4	0.0	0.0	11.850308325639963	0.0
33	1.5412428890906204E-4	0.0	0.0	12.116326848297003	0.0
34	1.5412428890906204E-4	0.0	0.0	12.407313505757314	0.0
35	1.5412428890906204E-4	0.0	0.0	12.722882987298618	0.0
36	1.5412428890906204E-4	0.0	0.0	13.00169382593511	0.0
37	1.5412428890906204E-4	0.0	0.0	13.281275286016148	0.0
38	1.5412428890906204E-4	0.0	0.0	13.622506461660812	0.0
39	1.5412428890906204E-4	0.0	0.0	14.111696954658175	0.0
40	1.5412428890906204E-4	0.0	0.0	14.428884741233025	0.0
41	1.5412428890906204E-4	0.0	0.0	14.761407894554326	0.0
42	1.5412428890906204E-4	0.0	0.0	15.098477714398445	0.0
43	1.5412428890906204E-4	0.0	0.0	15.464677024846377	0.0
44	1.5412428890906204E-4	0.0	0.0	15.76999724117523	0.0
45	1.5412428890906204E-4	0.0	0.0	16.082484236938352	0.0
46	2.3118643336359306E-4	0.0	0.0	16.393198803379022	0.0
47	2.3118643336359306E-4	0.0	0.0	16.727956758889505	0.0
48	2.3118643336359306E-4	0.0	0.0	17.04830409338699	0.0
49	2.3118643336359306E-4	0.0	0.0	17.386683969686835	0.0
50	2.3118643336359306E-4	0.0	0.0	17.717665880119046	0.0
51	3.082485778181241E-4	0.0	0.0	18.065832648764616	0.0
52	3.082485778181241E-4	0.0	0.0	18.48666901963081	0.0
53	3.082485778181241E-4	0.0	0.0	18.83707059046556	0.0
54	3.082485778181241E-4	0.0	0.0	19.17136617310932	0.0
55	3.082485778181241E-4	0.0	0.0	19.51390740520971	0.0
56	3.082485778181241E-4	0.0	0.0	19.838647281941103	0.0
57	3.082485778181241E-4	0.0	0.0	20.17517766677404	0.0
58	3.082485778181241E-4	0.0	0.0	20.493136074793433	0.0
59	3.082485778181241E-4	0.0	0.0	20.833750753282462	0.0
60	3.082485778181241E-4	0.0	0.0	21.29242463707583	0.0
61	3.082485778181241E-4	0.0	0.0	21.67642530289276	0.0
62	3.082485778181241E-4	0.0	0.0	22.114446531972312	0.0
63	3.082485778181241E-4	0.0	0.0	22.526189569792873	0.0
64	3.8531072227265513E-4	0.0	0.0	22.929995206734613	0.0
65	3.8531072227265513E-4	0.0	0.0	23.299045816527364	0.0
66	3.8531072227265513E-4	0.0	0.0	23.652144562418027	0.0
67	3.8531072227265513E-4	0.0	0.0	24.03876534114641	0.0
68	3.8531072227265513E-4	0.0	0.0	24.4723169658476	0.0
69	3.8531072227265513E-4	0.0	0.0	25.118328922809933	0.0
70	3.8531072227265513E-4	0.0	0.0	25.513118288850496	0.0
71	3.8531072227265513E-4	0.0	0.0	25.91152957568042	0.0
72	3.8531072227265513E-4	0.0	0.0	26.32974583363516	0.0
73	4.623728667271861E-4	0.0	0.0	26.697332262683272	0.0
74	4.623728667271861E-4	0.0	0.0	27.13774241824092	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCG	15	0.0013098293	80.0	1
TAATACG	60	0.0	80.0	1
TTGCGCA	50	0.0	80.0	1
CGAACGG	40	4.1836756E-11	79.99999	2
TACTACG	40	4.1836756E-11	79.99999	1
GGTACGT	145	0.0	77.24138	8
GCGTAAG	190	0.0	75.78948	1
TACGGGA	280	0.0	75.71429	4
GCGCGAC	90	0.0	75.55555	9
CGTAAGG	265	0.0	72.452835	2
CGTTTTT	4605	0.0	71.92182	1
ACGGGAT	260	0.0	70.76923	5
GTAGGGT	1490	0.0	70.067116	4
TATTACG	40	3.7143764E-9	69.99999	1
TAGGGAC	1315	0.0	69.961975	5
TACGGGT	75	0.0	69.333336	4
AGGGTAA	1510	0.0	68.874176	6
TAGGGTA	1710	0.0	68.77193	5
CTAGTCG	35	1.1815973E-7	68.57143	1
GCGATAT	35	1.1815973E-7	68.57143	9
>>END_MODULE